BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0057.0016.p (538 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_623393.1| Glucan phosphorylase [Thermoanaerobacter tengco... 779 0.0 ref|NP_347493.1| Glucan phosphorylase [Clostridium acetobutylicu... 736 0.0 gb|AAN87532.1| Glycogen phosphorylase [Heliobacillus mobilis] 605 e-172 ref|NP_486846.1| probable alpha-glucan phosphorylase [Nostoc sp.... 380 e-104 ref|ZP_00017654.1| hypothetical protein [Chloroflexus aurantiacus] 379 e-104 >ref|NP_623393.1| Glucan phosphorylase [Thermoanaerobacter tengcongensis] gb|AAM24997.1| Glucan phosphorylase [Thermoanaerobacter tengcongensis] Length = 540 Score = 779 bits (2011), Expect = 0.0, Method: Compositional matrix adjust. Identities = 367/537 (68%), Positives = 439/537 (81%), Gaps = 3/537 (0%) Query: 4 KQLPSVAYFSMEYGLHSDFKMYAGGLGILAGDYIKGARDIQAPIIPIGLKWKQGYTDQKI 63 K+LP VAYF MEYGL S+FK+YAGGLGILAGDY+K A+++ P++ IG+ WKQGYT+Q I Sbjct: 4 KKLPRVAYFCMEYGLESNFKLYAGGLGILAGDYLKAAKEMGLPVVGIGILWKQGYTEQHI 63 Query: 64 DASGKPYDSYHNNV--YDFLEDTGVKVTVKVRKIDVVCKVWKTDHFGNSPLYLLDTDIPE 121 G PYD+Y N YDFL+DTGVKV VK+R DV CKVW D F N+PLYLLDTDIPE Sbjct: 64 GEDGYPYDAYRNYTRNYDFLKDTGVKVKVKIRNKDVYCKVWLVDSFDNAPLYLLDTDIPE 123 Query: 122 NGDAWITGQLYGWFGEERIAQEIVLGIGGVKAMRALQIPIDVYHFNEGHAALAAIELIRE 181 NGD WITGQLYGWFGEER+AQEIVLGIGGV+A+RAL I +D+YHFNEGHA LA IELIRE Sbjct: 124 NGDRWITGQLYGWFGEERVAQEIVLGIGGVRALRALGIDVDIYHFNEGHAVLAGIELIRE 183 Query: 182 KMSG-GSSFEEAWKATREEVVFTTHTPIKEGNETHPLDRLEYMGAFNGLTRDQMERIGGD 240 KM G SFEEAW+ATR+E+VFTTHTP+KEGNE+H L+ L YMGA NGLT +Q+ +IGG Sbjct: 184 KMENQGMSFEEAWEATRKEIVFTTHTPVKEGNESHDLELLMYMGANNGLTIEQLAQIGGV 243 Query: 241 PFNMTVAGLRLSRISNAVAQLHADTANKMWKEVAGRSEIIGITNAIHTPTWVDERMTRAF 300 PFNMTVAGLRLS+I+N V++LH TANKMW+ + ++ II ITN IH TWVD+R+T A+ Sbjct: 244 PFNMTVAGLRLSKIANGVSKLHGQTANKMWQHIENKAPIISITNGIHRGTWVDKRITEAY 303 Query: 301 EEGGDLWATHKEIKGELISFIEERSGISLNADNLLIGFSRRAAPYKRSDLIFSQPEIIEP 360 ++G L TH +K ELI F+ +++G+ L+AD LLIGFSRRAAPYKRSDLIF+ + I Sbjct: 304 KKGTGLLETHNLLKKELIDFVYKKTGVKLDADKLLIGFSRRAAPYKRSDLIFTNEKAIGE 363 Query: 361 YLESGRIQIVFSGKAHPLDDTGKKIVSNLVAMMKKYPKSVVFLENYDMTIGAMLTRGSDI 420 YL +IQIVFSGK HPLDD GK+IV+ +V M KKYP+SVVFLE+YDMTIG MLTRG+D+ Sbjct: 364 YLRDRKIQIVFSGKGHPLDDVGKEIVARIVKMTKKYPESVVFLEDYDMTIGKMLTRGADV 423 Query: 421 WLNNPRRPLEASGTSGMKAAMNGVLNCSILDGWWPEACIDGENGWQIGDGFETTDFAVLD 480 WLNNPRRPLEASGTSGMKAAMNGVLN SILDGWWPEACIDG NGWQ GDGFE+ +F LD Sbjct: 424 WLNNPRRPLEASGTSGMKAAMNGVLNLSILDGWWPEACIDGVNGWQFGDGFESDNFEELD 483 Query: 481 KHDSDALYDTLLKRVLPTFYKDKDKWVQMMHKSIETTRTEFASKRMLEEYYNRMYIK 537 KHD++ALYD LL +V+PT+Y DK KW +MM +SI TT F++ RML+EYY+ MY K Sbjct: 484 KHDAEALYDVLLNKVVPTYYNDKKKWEEMMKESIRTTFEAFSANRMLQEYYDLMYTK 540 >ref|NP_347493.1| Glucan phosphorylase [Clostridium acetobutylicum] pir||F97005 glucan phosphorylase [imported] - Clostridium acetobutylicum gb|AAK78833.1| Glucan phosphorylase [Clostridium acetobutylicum] Length = 540 Score = 736 bits (1900), Expect = 0.0, Method: Compositional matrix adjust. Identities = 345/534 (64%), Positives = 423/534 (79%), Gaps = 2/534 (0%) Query: 6 LPSVAYFSMEYGLHSDFKMYAGGLGILAGDYIKGARDIQAPIIPIGLKWKQGYTDQKIDA 65 LP +AYFSMEY + + K YAGGLGILAGDY+KG++D PII IG+KWKQGYTDQ ID Sbjct: 7 LPRIAYFSMEYAIDASIKTYAGGLGILAGDYLKGSKDYNYPIIGIGIKWKQGYTDQLIDE 66 Query: 66 SGKPYDSYHNNVYDFLEDTGVKVTVKVRKIDVVCKVWKTDHFGNSPLYLLDTDIPENGDA 125 + YD+YHN YDFL+DT +KV VK+ + DV CK+W D F +PLYLLDTD+PEN + Sbjct: 67 NSNVYDTYHNYKYDFLKDTKLKVKVKIYEKDVTCKIWLLDKFKLAPLYLLDTDLPENDNR 126 Query: 126 WITGQLYGWFGEERIAQEIVLGIGGVKAMRALQIPIDVYHFNEGHAALAAIELIREKMSG 185 WIT QLYG F + R+AQEIVLGIGGV+A+RAL I +DVYHFNEGHA A EL+REKM+ Sbjct: 127 WITNQLYGGFEDSRLAQEIVLGIGGVRALRALDINVDVYHFNEGHALFAGFELVREKMNT 186 Query: 186 GSSFEEAWKATREEVVFTTHTPIKEGNETHPLDRLEYMGAFNGLTRDQMERIGGDPFNMT 245 G F+EA K ++EE+VFTTHTPI EGNE+H + R+ +GA NG T +Q+ +GG PFNMT Sbjct: 187 GIPFDEAVKKSKEEIVFTTHTPIIEGNESHSISRMISIGANNGFTIEQLISLGGCPFNMT 246 Query: 246 VAGLRLSRISNAVAQLHADTANKMWKEVAGRSEIIGITNAIHTPTWVDERMTRAFEE--G 303 V LRLSRISNAV++LH +TANKMWK + +S+II ITNAIH PTWVDE M +A E+ Sbjct: 247 VGALRLSRISNAVSKLHGETANKMWKNIKNKSKIISITNAIHIPTWVDEDMLKAAEDSNS 306 Query: 304 GDLWATHKEIKGELISFIEERSGISLNADNLLIGFSRRAAPYKRSDLIFSQPEIIEPYLE 363 +LW H + K +LISF++ER+ I LN ++LLIGFSRRAAPYKRS+LIFS +II P L+ Sbjct: 307 NNLWTNHIKNKRKLISFVKERNEIELNENSLLIGFSRRAAPYKRSNLIFSDMKIISPLLK 366 Query: 364 SGRIQIVFSGKAHPLDDTGKKIVSNLVAMMKKYPKSVVFLENYDMTIGAMLTRGSDIWLN 423 G+IQIVFSGKAHPLDDTGK I+ NLV K+YP SVVFLENYDMTIG +LT+GSD+WLN Sbjct: 367 DGKIQIVFSGKAHPLDDTGKDIIKNLVNFSKQYPSSVVFLENYDMTIGKLLTQGSDVWLN 426 Query: 424 NPRRPLEASGTSGMKAAMNGVLNCSILDGWWPEACIDGENGWQIGDGFETTDFAVLDKHD 483 NPRRPLEASGTSGMKAAMNGVLN S LDGWWPEAC DG NGWQ GDG ++ D +DKHD Sbjct: 427 NPRRPLEASGTSGMKAAMNGVLNLSTLDGWWPEACRDGINGWQFGDGLQSDDIYFIDKHD 486 Query: 484 SDALYDTLLKRVLPTFYKDKDKWVQMMHKSIETTRTEFASKRMLEEYYNRMYIK 537 ++ALY+ L+ +V+PTFY ++ KW+ MM SI +T+ F+ KRMLEEYY+++YIK Sbjct: 487 ANALYEVLMNKVIPTFYLERKKWISMMKNSILSTKDYFSVKRMLEEYYSKIYIK 540 >gb|AAN87532.1| Glycogen phosphorylase [Heliobacillus mobilis] Length = 610 Score = 605 bits (1561), Expect = e-172, Method: Compositional matrix adjust. Identities = 289/533 (54%), Positives = 371/533 (69%), Gaps = 2/533 (0%) Query: 5 QLPSVAYFSMEYGLHSDFKMYAGGLGILAGDYIKGARDIQAPIIPIGLKWKQGYTDQKID 64 +LP VAYF MEYGLH + +YAGGLG+LAGDY+K A D P++ IG+ W Q YT Q I Sbjct: 44 KLPRVAYFCMEYGLHEELPIYAGGLGVLAGDYLKAAHDSGLPMVAIGILWNQDYTHQFIG 103 Query: 65 ASGKPYDSYHNNVYDFLEDTGVKVTVKVRKIDVVCKVWKTDHFGNSPLYLLDTDIPENGD 124 G+PYD+Y N +D ++DTGV V V+VR DVVCKV K D +GN PLYLLDT+ P + Sbjct: 104 EQGRPYDTYPNIRFDSVKDTGVTVQVRVRGADVVCKVHKVDSYGNVPLYLLDTNFPGSEH 163 Query: 125 AWITGQLYGWFGEERIAQEIVLGIGGVKAMRALQIPIDVYHFNEGHAALAAIELIREKMS 184 W+T +LYG G++R+A EI+LGIGG++A+RAL I +D+YH NEGHA ELIREKM+ Sbjct: 164 GWMTNRLYGGSGQDRVAAEIILGIGGIRALRALGIEVDLYHLNEGHATFCGFELIREKMT 223 Query: 185 G-GSSFEEAWKATREEVVFTTHTPIKEGNETHPLDRLEYMGAFNGLTRDQMERIGGDPFN 243 G +F +AW+ R+E++FTTHTP++ GNE H D L M A+NGLT +Q++ IGGDPFN Sbjct: 224 HYGMTFHQAWEDVRKELIFTTHTPVEAGNEVHGHDLLRLMEAYNGLTYEQVKDIGGDPFN 283 Query: 244 MTVAGLRLSRISNAVAQLHADTANKMWKEVAGRSEIIGITNAIHTPTWVDERMTRAFEEG 303 MTVA LRLS I+N V++LH TA KMW +V + II +TN +H TW D ++ AFE Sbjct: 284 MTVAALRLSHIANGVSKLHGQTACKMWSDVHNAACIISVTNGVHNRTWQDHSISDAFEHK 343 Query: 304 GDLWATHKEIKGELISFIEERSGISLNADNLLIGFSRRAAPYKRSDLIFSQPEIIEPYLE 363 GDLW H K L+ +IE + LN DNL++GF+RRAAPYKRSDLIF E+IEP L+ Sbjct: 344 GDLWEAHLNNKYRLVDYIERHRNVRLNPDNLIVGFARRAAPYKRSDLIFRTAEVIEPLLQ 403 Query: 364 SGRIQIVFSGKAHPLDDTGKKIVSNLVAMMKKYPKSVVFLENYDMTIGAMLTRGSDIWLN 423 ++Q+VFSGKAHP D GK I+ LV M +KY SVVFLENY+M + + RG D+WLN Sbjct: 404 ERKLQLVFSGKAHPNDGIGKDIIQQLVRMSEKYGNSVVFLENYNMEVARYMVRGCDVWLN 463 Query: 424 NPRRPLEASGTSGMKAAMNGVLNCSILDGWWPEACIDGENGWQIGDGFET-TDFAVLDKH 482 NPRRPLEASGTSGMKAAMNGVLN S +DGW E +GW I E D+ Sbjct: 464 NPRRPLEASGTSGMKAAMNGVLNLSTVDGWVGEGLQHQVSGWLINPDIEAHLQEWEQDER 523 Query: 483 DSDALYDTLLKRVLPTFYKDKDKWVQMMHKSIETTRTEFASKRMLEEYYNRMY 535 D LY LL V+PT+Y +++ W+QMM SI+ R +F+S RM+ EY++ MY Sbjct: 524 DLRELYRVLLNEVIPTYYNNRNYWIQMMRASIDMARWQFSSHRMIREYFDLMY 576 >ref|NP_486846.1| probable alpha-glucan phosphorylase [Nostoc sp. PCC 7120] pir||AG2156 hypothetical protein all2806 [imported] - Nostoc sp. (strain PCC 7120) dbj|BAB74505.1| ORF_ID:all2806~probable alpha-glucan phosphorylase [Nostoc sp. PCC 7120] Length = 737 Score = 380 bits (977), Expect = e-104, Method: Compositional matrix adjust. Identities = 233/610 (38%), Positives = 332/610 (54%), Gaps = 84/610 (13%) Query: 9 VAYFSMEYGLHSDFKMYAGGLGILAGDYIKGARDIQAPIIPIGLKWKQGYTDQKIDASGK 68 VAYF E+G+H +Y+GGLGILAGD++K + D+ P++ IGL ++QGY Q++ G Sbjct: 114 VAYFCAEFGIHESLPIYSGGLGILAGDHLKSSSDLGVPLVGIGLLYRQGYFRQRLSRQGW 173 Query: 69 PYDSYHNNVYDFL------EDTGVKVTVK--VRKIDVVCKVWKTDHFGNSPLYLLDTDIP 120 D Y +N + + + G +T+K +R+ V ++W+ G LYLLD+D Sbjct: 174 QEDYYVDNSFHRMPIELIKNEHGEPLTIKLEIRQRQVTVQIWRV-QVGRVSLYLLDSDRH 232 Query: 121 ENG--DAWITGQLYGWFGEERIAQEIVLGIGGVKAMRALQIPIDVYHFNEGHAALAAIEL 178 +N D W+TG LYG E RIAQE+VLGIGGV+A++AL I VYH NEGHAA +E+ Sbjct: 233 DNDPIDRWLTGHLYGGNTETRIAQEVVLGIGGVRALQALGIKPSVYHLNEGHAAFCTLEI 292 Query: 179 IR-EKMSGGSSFEEAWKATREEVVFTTHTPIKEGNETHPLDRLEYMGAFN----GLTRDQ 233 R E G SF + + R VFTTHTP+ G++ D ++ A L+R+Q Sbjct: 293 ARLEIERTGKSFYDIEASVRNSCVFTTHTPVPAGHDVFSPDLIDSFFAHYWTQLRLSREQ 352 Query: 234 MERIGG-------DPFNMTVAGLRLSRISNAVAQLHADTANKMWKEV-AGRSE----IIG 281 +G +PF MTV LR+SR N V++LH + KMW + RSE I Sbjct: 353 FLALGARRLGDPWEPFGMTVLALRMSRACNGVSELHGQVSRKMWTVLYPQRSEEKVPIGY 412 Query: 282 ITNAIHTPTW---------------------VDERMTRAFEEGGD--LWATHKEIKGELI 318 ITN +H PTW +D +M ++ D LW+ H +K L+ Sbjct: 413 ITNGVHAPTWTAPLLADLYNQYLGADWRTQAIDPKMWAKVDDIPDEELWSRHLILKERLV 472 Query: 319 SFIE-----------------ERSGISLNADNLLIGFSRRAAPYKRSDLIFSQPE-IIEP 360 ++ + S L+ L IGF+RR +PYKR DLI E + Sbjct: 473 AYTRYKVRKSREHRGEDQRLIQASDSLLDPKILTIGFARRFSPYKRGDLILRDAERALRI 532 Query: 361 YLESGR-IQIVFSGKAHPLDDTGKKIVSNLVAMMKK--YPKSVVFLENYDMTIGAMLTRG 417 + + R +QIVF+GKAHP D+ GK+I+ L+ V F+E+YD+ G L +G Sbjct: 533 FSNAERPVQIVFAGKAHPADEEGKRIIQRLMEWCHNSGIINRVAFIEDYDIYTGQKLVQG 592 Query: 418 SDIWLNNPRRPLEASGTSGMKAAMNGVLNCSILDGWWPE-----ACIDGENGWQIGDGFE 472 D+WLNNPRRPLEASGTSG K NG +NCS+LDGWW E A G NGW IG+ Sbjct: 593 VDVWLNNPRRPLEASGTSGQKVCFNGGINCSVLDGWWCEGYQADANGKGLNGWAIGEDAH 652 Query: 473 TTDFAVLDKHDSDALYDTLLKRVLPTFYKDKD------KWVQMMHKSIETTRTEFASKRM 526 T+D + D+ DS +LY L + ++P +Y D+D +WVQ+M SI+T F + RM Sbjct: 653 TSDQELQDRIDSQSLYKLLEEEIVPLYY-DQDANGIPHRWVQVMKASIKTNAPLFNTDRM 711 Query: 527 LEEYYNRMYI 536 + +Y ++Y+ Sbjct: 712 IADYVTQVYV 721 >ref|ZP_00017654.1| hypothetical protein [Chloroflexus aurantiacus] Length = 716 Score = 379 bits (974), Expect = e-104, Method: Compositional matrix adjust. Identities = 237/599 (39%), Positives = 335/599 (55%), Gaps = 73/599 (12%) Query: 9 VAYFSMEYGLHSDFKMYAGGLGILAGDYIKGARDIQAPIIPIGLKWKQGYTDQKIDASGK 68 +AYFS E+GLH +Y+GGLGIL+GD+IK A D+ P++ +G + QGY Q++D SG Sbjct: 109 IAYFSAEFGLHESLPIYSGGLGILSGDHIKEASDMGIPLVGVGFIYPQGYFRQRLDQSGW 168 Query: 69 PYDSYHN-NVYDFL-------EDTGVKVTVKVRKIDVVCKVWKTDHFGNSPLYLLDTDIP 120 + Y+ N D E V V V++ + KV+K G + L L+DTDI Sbjct: 169 QFAEYNKLNFADVPALPALDPEGREVVVEVELPGRTIYAKVYKF-QVGRNELLLMDTDIH 227 Query: 121 ENG--DAWITGQLYGWFGEERIAQEIVLGIGGVKAMRALQIPIDVYHFNEGHAALAAIEL 178 N D ++ +LYG E RI+QE+VLG+GGV+A+R L V+H NEGH+A +EL Sbjct: 228 PNSPQDRELSARLYGGDQEMRISQELVLGVGGVRALRRLGYKPTVWHMNEGHSAFLVLEL 287 Query: 179 IREKMSGGSSFEEAWKATREEVVFTTHTPIKEGNETHPLDRLE-YMGAF---NGLTRDQM 234 RE ++ G SFE+A + + VFTTHTP+ GN+ PL +E + +F LTRD+ Sbjct: 288 CRELVAQGYSFEQAMEQVKAHCVFTTHTPVPAGNDAFPLPMIEKFFWSFWPQLNLTRDEF 347 Query: 235 ------ERIGGDPFNMTVAGLRLSRISNAVAQLHADTANKMWKEV-AGRSE----IIGIT 283 E+ G F MT LR S N V++LH A MW+ + GRS+ I IT Sbjct: 348 MSLALQEQQWGPTFAMTALALRFSAFHNGVSKLHGHVARGMWQWLYPGRSQDEVPITSIT 407 Query: 284 NAIHTPTWVDERMT--------RAFEEGGD---------------LWATHKEIKGELISF 320 N +HT TW+ M + +E+ D W T +++K +LI+F Sbjct: 408 NGVHTATWLAPEMRTLYNSYLGKNWEDNLDDPQIWRKVYQIPDETFWRTRQKLKSDLIAF 467 Query: 321 IEERS-------GIS------LNADNLLIGFSRRAAPYKRSDLIFSQPEIIEPYL-ESGR 366 +R GI L + L IGF+RR A YKR+ L+F E ++ L G+ Sbjct: 468 SRQRLQQHYMRLGIPAPVWPVLEENILTIGFARRFATYKRATLLFKDVERLKYILNRPGK 527 Query: 367 -IQIVFSGKAHPLDDTGKKIVSNL--VAMMKKYPKSVVFLENYDMTIGAMLTRGSDIWLN 423 IQI+F+GKAHP DD GK + ++ +AM ++FLE YD+ +G L +G D+WLN Sbjct: 528 PIQIIFAGKAHPKDDPGKHFIQDVYRMAMQPGLAGRIIFLEEYDIAVGRALVQGVDVWLN 587 Query: 424 NPRRPLEASGTSGMKAAMNGVLNCSILDGWWPEACIDGENGWQIGDGFETTDFAVLDKHD 483 NPRRP EASGTSGMKA++NG NCS+LDGWWPEA +G NGW IGD E D +D Sbjct: 588 NPRRPYEASGTSGMKASLNGAPNCSVLDGWWPEA-YNGRNGWAIGDEREYASQEEQDWND 646 Query: 484 SDALYDTLLKRVLPTFYKDKD------KWVQMMHKSIETTRTEFASKRMLEEYYNRMYI 536 + +LY L + P FY ++D +WVQ+ ++I T F+++RML +Y MY+ Sbjct: 647 AQSLYHLLEHEIAPRFYDNRDANGIPVEWVQICKEAIATVAPLFSTRRMLADYLREMYM 705 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.136 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 867,084,977 Number of extensions: 38651535 Number of successful extensions: 84814 Number of sequences better than 1.0e-05: 129 Number of HSP's gapped: 84425 Number of HSP's successfully gapped: 131 Length of query: 538 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 407 Effective length of database: 401,559,609 Effective search space: 163434760863 Effective search space used: 163434760863 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)