BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0054.0015.p (329 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00099211.1| COG0697: Permeases of the drug/metabolite tra... 213 7e-54 gb|EAJ17027.1| unknown [environmental sequence] 115 1e-24 gb|EAC60761.1| unknown [environmental sequence] 115 1e-24 gb|EAJ09984.1| unknown [environmental sequence] 112 1e-23 gb|EAK15517.1| unknown [environmental sequence] 109 9e-23 >ref|ZP_00099211.1| COG0697: Permeases of the drug/metabolite transporter (DMT) superfamily [Desulfitobacterium hafniense] Length = 324 Score = 213 bits (541), Expect = 7e-54, Method: Compositional matrix adjust. Identities = 129/300 (43%), Positives = 182/300 (60%), Gaps = 2/300 (0%) Query: 21 WLVVLGAALWGVDPLFRIILLKSLTSSQIVLLEHVVLFLAAAPVLWRHRAELKGVRLRQA 80 WL+ LGAA+WG+D +F + LL+ ++SSQIV LEH++LFL AAPVL R ELK + Sbjct: 17 WLIALGAAMWGLDGVFIVSLLQHVSSSQIVWLEHLLLFLFAAPVLIWKRQELKQLNGGDW 76 Query: 81 AALLIVSWGGSAVATILFTKALASGDFNAVLLLQKLQPLFAIGLAAVILKERLPKNFGPL 140 A+L + WGGSA+A+ILFT G+ N VLLLQK+QP+F I LA+ +LKER+ K+F L Sbjct: 77 LAVLFIGWGGSALASILFTAGFTYGNPNVVLLLQKVQPIFVILLASWMLKERMRKDFYAL 136 Query: 141 IIVALAGTYLLTFGWTIPFGHVNSFISIXXXXXXXXXXXXXXXTVMGRYLLGSMKYETVT 200 +VAL G YLLTFG +P V+ + TVMG+ L+G + + T+T Sbjct: 137 FVVALIGAYLLTFGLHLPTSGVSLAQLLGSLCAIGAAALWGGSTVMGKRLIGKLSFTTLT 196 Query: 201 SLRFILALPLLFVITSMEGAPWQIHGGLGTSAAIGINXXXXXXXXXXXXXXXYYKGLNTT 260 +LRF +ALPLL +I ++ W G A + N YY+GLN Sbjct: 197 ALRFAVALPLLTIIVLVQQPNWADLGQSLHLAPVWANLLYQTLVPSLLSLLLYYRGLNGV 256 Query: 261 KASYATLAELSFPMTGILINWVVYHQLVTLPQIIGFALIWTALFFISS--QQSRQAVPAS 318 +AS+AT+AEL+FP TG+L+NW++ HQ + L Q +GFA++W + +S + AV S Sbjct: 257 RASHATIAELAFPATGLLVNWLILHQTIDLGQWLGFAIVWLVVLQLSRMPNDPKSAVKTS 316 >gb|EAJ17027.1| unknown [environmental sequence] Length = 297 Score = 115 bits (289), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 94/277 (33%), Positives = 139/277 (50%), Gaps = 10/277 (3%) Query: 23 VVLGAALWGVDPLFRIILLKSLTSSQIVLLEHVVLFLAAAPVLWRHRAELKGVRLRQAAA 82 +V A LW D L R L ++ S IV +EH++ + P + + R E+K + R + Sbjct: 10 IVFAAILWSFDGLLRQAL-YNVPSLIIVTIEHIIGAVLFIPFILQARKEIKQINQRTWVS 68 Query: 83 LLIVSWGGSAVATILFTKALASGDF---NAVLLLQKLQPLFAIGLAAVILKERLPKNFGP 139 + +S G + T +T AL+ ++ + V+LLQKLQPLFAI LA+VILKE+L K F Sbjct: 69 VFWISLCGGILGTFFYTSALSYVNYINLSVVVLLQKLQPLFAISLASVILKEKLSKKFLF 128 Query: 140 LIIVALAGTYLLTFGWTIPFGHVNSFISIXXXXXXXXXXXXXXXTVMGRYLLGSMKYETV 199 L VA+ G++L+TFG P I TV+G+Y L ++ ++ V Sbjct: 129 LASVAIIGSFLVTFGLQ-PLSEWGDKTIIASLFALLAAFSWGSSTVIGKYALKNLSFQLV 187 Query: 200 TSLRFIL-ALPLLFVITSMEGAPWQIHGGLGTSAAIGINXXXXXXXXXXXXXXXYYKGLN 258 TSLR I+ A+ L +I E QI L + I YY GL Sbjct: 188 TSLRLIITAVICLIIIIGTEDL--QIIVTLTKPQWLFI--FLISISTGSIALFIYYYGLK 243 Query: 259 TTKASYATLAELSFPMTGILINWVVYHQLVTLPQIIG 295 AS+ATL EL +P++ + I+W + + L QIIG Sbjct: 244 NLPASHATLYELFWPLSAMFIDWFFRSRTLELAQIIG 280 >gb|EAC60761.1| unknown [environmental sequence] Length = 295 Score = 115 bits (289), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 93/302 (30%), Positives = 154/302 (50%), Gaps = 30/302 (9%) Query: 23 VVLGAALWGVDPLFRIILLKSLTSSQIVLLEHVVLFLAAAPVLWRHRAELKGVRLRQAAA 82 V+ A LW +D R L ++ S IV LEH + + P L + +K + R+ + Sbjct: 10 VIFAALLWSIDGFLRQ-ELNTVPSILIVSLEHSIGAIIFLPFLVKSWKSIKTLGQREWVS 68 Query: 83 LLIVSWGGSAVATILFTKALASGDF---NAVLLLQKLQPLFAIGLAAVILKERLPKNFGP 139 +L ++ G + T +TKAL ++ + V+LLQKLQP+FAI L+++ILKE+L F Sbjct: 69 ILWIAIFGGILGTFFYTKALGYVNYINLSVVVLLQKLQPVFAIALSSIILKEKLSNKFIS 128 Query: 140 LIIVALAGTYLLTFGWTIPFGHVNSFISIXXXXXXXXXXXXXXXTVMGRYLLGSMKYETV 199 L + A+ G YL+TFG T+ F + I I TV+G++ L S+ +ETV Sbjct: 129 LSLSAIIGAYLVTFGSTLEFEWNDKTI-IASLLALLSAFCWGSSTVLGKHALKSLPFETV 187 Query: 200 TSLRF----ILALPLLF------VITSMEGAPWQ-IHGGLGTSAAIGINXXXXXXXXXXX 248 TSLR AL LF ++ ++ WQ I + +S +I + Sbjct: 188 TSLRLTVTAFFALIALFATAEFSMVLELKSVQWQKILMIVFSSGSIAL------------ 235 Query: 249 XXXXYYKGLNTTKASYATLAELSFPMTGILINWVVYHQLVTLPQIIGFALIWTALFFISS 308 YY GL AS+ T+ EL++P++ +L++W V QL+ Q++G ++ ++ +S Sbjct: 236 --AIYYYGLQNLPASHTTIYELAWPLSALLLDWAVRDQLLNATQLLGALILLVSMIVLSK 293 Query: 309 QQ 310 + Sbjct: 294 EN 295 >gb|EAJ09984.1| unknown [environmental sequence] Length = 299 Score = 112 bits (279), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 86/280 (30%), Positives = 139/280 (49%), Gaps = 16/280 (5%) Query: 23 VVLGAALWGVDPLFRIILLKSLTSSQIVLLEHV---VLFLAAAPVLWRHRAELKGVRLRQ 79 ++ A LW D R L ++ S IV +EH+ VLF+ P +++ R E+ + R Sbjct: 10 IITAAILWSFDGFLRQSLY-NVPSLIIVAIEHIFGAVLFI---PFIFKARKEINNINQRT 65 Query: 80 AAALLIVSWGGSAVATILFTKALASGDF---NAVLLLQKLQPLFAIGLAAVILKERLPKN 136 ++ +S G + T +T AL+ ++ + V+LLQKLQPLFAI LA+VILKE+L K Sbjct: 66 WVSVFWISICGGILGTFFYTSALSYVNYINLSVVVLLQKLQPLFAISLASVILKEKLSKK 125 Query: 137 FGPLIIVALAGTYLLTFGWTIPFGHVNSFISIXXXXXXXXXXXXXXXTVMGRYLLGSMKY 196 F L +A+ G++L+TFG P I TV+G++ L + + Sbjct: 126 FLLLATIAIIGSFLVTFGLQ-PIAEWEDKTIIASLFALLAAFSWGSSTVLGKHALKKLSF 184 Query: 197 ETVTSLRFIL-ALPLLFVITSMEGAPWQIHGGLGTSAAIGINXXXXXXXXXXXXXXXYYK 255 + VTSLR I+ ++ LF+I + +H + + ++ YY Sbjct: 185 QIVTSLRLIITSIVCLFII----AGSYDLHIITELTKSQWLSIFIISISTGSVALFIYYY 240 Query: 256 GLNTTKASYATLAELSFPMTGILINWVVYHQLVTLPQIIG 295 GL AS+ TL EL +P++ + I+W + + QIIG Sbjct: 241 GLKKIPASHTTLYELFWPLSAMFIDWFYRSKTLETAQIIG 280 >gb|EAK15517.1| unknown [environmental sequence] Length = 291 Score = 109 bits (272), Expect = 9e-23, Method: Compositional matrix adjust. Identities = 95/308 (30%), Positives = 152/308 (49%), Gaps = 42/308 (13%) Query: 23 VVLGAALWGVDPLFRIILLKSLTSSQIVLLEHVVLFLAAAPVLWRHRAELKGVRLRQAAA 82 VV A LW +D R L S+ + IV LEH + L P+L + +K + R+ A Sbjct: 3 VVFAALLWSIDGFLRQ-ELYSIPAIVIVALEHSIGALIFIPILLKSWKTIKVLGQREWMA 61 Query: 83 LLIVSWGGSAVATILFTKALASGDF---NAVLLLQKLQPLFAIGLAAVILKERLPKNFGP 139 +L ++ G + T +TKAL ++ + V+LLQKLQP+FAI L+++ILKE+L K F Sbjct: 62 ILWIAIFGGILGTFFYTKALGYINYINLSVVVLLQKLQPVFAIALSSIILKEKLSKRFIG 121 Query: 140 LIIVALAGTYLLTFG------WTIPFGHVNSFISIXXXXXXXXXXXXXXXTVMGRYLLGS 193 L IVA+ G YL+TFG W N I TV+G++ L Sbjct: 122 LAIVAILGGYLVTFGSASTLVW-------NDKAIIASLLAILSAFCWGSSTVLGKHALKI 174 Query: 194 MKYETVTSLRF----ILALPLLFVITS------MEGAPWQ-IHGGLGTSAAIGINXXXXX 242 + YE VTSLR + AL +LF + + WQ + + +S A+ + Sbjct: 175 LPYEVVTSLRLSLTALFALLVLFATSEATTVLYLTTIQWQRVIMIVFSSGAVAL------ 228 Query: 243 XXXXXXXXXXYYKGLNTTKASYATLAELSFPMTGILINWVVYHQLVTLPQIIGFALIWTA 302 YY GL AS+ T+ EL++P++ + ++W++ QL++ QI G ++ + Sbjct: 229 --------AIYYYGLEKLPASHTTIYELAWPLSAVALDWIIRGQLLSTSQIFGALMLLVS 280 Query: 303 LFFISSQQ 310 + +S + Sbjct: 281 MVILSRES 288 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.326 0.138 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 390,208,997 Number of extensions: 13546960 Number of successful extensions: 46022 Number of sequences better than 1.0e-05: 19 Number of HSP's gapped: 46008 Number of HSP's successfully gapped: 19 Length of query: 329 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 203 Effective length of database: 415,135,104 Effective search space: 84272426112 Effective search space used: 84272426112 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 126 (53.1 bits)