BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0054.0007.p (367 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_242802.1| spore germination protein KB [Bacillus halodura... 237 3e-61 gb|AAB61395.1| germination protein 237 4e-61 ref|NP_388254.1| gerKB [Bacillus subtilis] >gi|1708044|sp|P49940... 230 5e-59 ref|NP_149186.1| Spore germination protein, GRKB [Clostridium ac... 157 4e-37 ref|NP_348154.1| Spore germination protein GerKB [Clostridium ac... 126 8e-28 >ref|NP_242802.1| spore germination protein KB [Bacillus halodurans] pir||H83891 spore germination protein KB gerKB [imported] - Bacillus halodurans (strain C-125) dbj|BAB05655.1| spore germination protein KB [Bacillus halodurans] Length = 373 Score = 237 bits (605), Expect = 3e-61, Method: Compositional matrix adjust. Identities = 130/354 (36%), Positives = 214/354 (60%), Gaps = 1/354 (0%) Query: 6 IGPSQVFAMIVLFELGTAIVVPIGLESGHAVWLSILMALPGGVLLYLIYNDLYRQFPNLI 65 I Q F+MIVLFELG+AIVV +GL++G W+++L+A+ G++LY + L+ FP+ Sbjct: 7 ISTYQFFSMIVLFELGSAIVVGVGLDAGQDAWMTVLIAMLAGLVLYCVLYYLHSHFPSDT 66 Query: 66 ISGYTRKILGNYIGWPLSLLYIPVLMFNGSRNLREAGDLLISASYDRTPILIINSIMVIA 125 Y +K+L IGW + LY ++ +R LR+ GDLL ++ TP+++IN +MV Sbjct: 67 WIAYLQKVLTVPIGWFIGFLYCLYFIYIATRVLRDFGDLLTTSMLTDTPLMVINLLMVFV 126 Query: 126 VMYILNKGIEVFARTAEIYFWIMMVMGLVCNGVVIIAGLVDFKNLFPLHYKDWVDALTSA 185 ++Y+L GI V ART E + I+ +G ++ L + KNL P+ W+ L A Sbjct: 127 IVYVLFHGINVLARTGEFFLIILFFIGFFSVTLIFSTDLYNIKNLMPVLENGWMPVLREA 186 Query: 186 YPNIWIFPFGELVCFTTVLPFLKNSGISRKMGVTAIILSGFLLCFTHAIEISVLGLDIYS 245 +P + FPFGE++ FT + FL + ++G+ AI +SG LL T + I VLG+DI + Sbjct: 187 FPVTFTFPFGEIIVFTMLFHFLNKKEMGLRIGLLAIGMSGLLLALTTILYIIVLGVDITN 246 Query: 246 RAAFPIYTTITLVNVANFIQRLDALVILTLIIGVFFKMSIYCFAAAAITADLFKVKDYRR 305 RA FP+ T + + A+FI+RLDAL + L++ +FFK++I+ +A A ++LFKV +++ Sbjct: 247 RATFPLLTMVGKIQAADFIERLDALAVTVLVVAMFFKVAIFFYAGVAGFSELFKVDKFQK 306 Query: 306 LVVPVGVIVLISSFISVENYTSHLNEGKLFLKYFLPVICAV-VPILLFLVHRIR 358 L+ G I++ +S N++ HL EG + Y+L + V +P+++ + IR Sbjct: 307 LLPLFGAILVFNSVHIAANFSEHLKEGLEVVPYYLHLPFQVGIPLIVLAIVLIR 360 >gb|AAB61395.1| germination protein Length = 329 Score = 237 bits (604), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 123/329 (37%), Positives = 203/329 (61%) Query: 1 MKKEVIGPSQVFAMIVLFELGTAIVVPIGLESGHAVWLSILMALPGGVLLYLIYNDLYRQ 60 M+K I +Q+F ++VLFELG+A++VP+ + + WL+IL+ + +LIY+ LY Sbjct: 1 MEKAKISATQLFILMVLFELGSALLVPLAMGAKQDAWLAILIGMLCSFFFFLIYHKLYSY 60 Query: 61 FPNLIISGYTRKILGNYIGWPLSLLYIPVLMFNGSRNLREAGDLLISASYDRTPILIINS 120 +P+L+ + Y +KILG +G L+ +YI +++ SR LR+ G++L++ +Y TP+ I N+ Sbjct: 61 YPDLLPTEYMQKILGKVLGTLLAFIYIVYFLYDASRVLRDFGEMLLTFAYPDTPLFIANA 120 Query: 121 IMVIAVMYILNKGIEVFARTAEIYFWIMMVMGLVCNGVVIIAGLVDFKNLFPLHYKDWVD 180 ++++ ++YI KGIE AR+ E+ F M + + ++I +GL+DFKNL P+ + + Sbjct: 121 LLMLVIIYITRKGIEAIARSGELLFIFMYFLAIAGFILIISSGLIDFKNLQPVLKEGILP 180 Query: 181 ALTSAYPNIWIFPFGELVCFTTVLPFLKNSGISRKMGVTAIILSGFLLCFTHAIEISVLG 240 A FPF E + FT +LP+L N + + A LSG L T I ISVLG Sbjct: 181 VAKVALTETLYFPFTEAIVFTMLLPYLSNPKKANVTMLCATGLSGINLIITMIINISVLG 240 Query: 241 LDIYSRAAFPIYTTITLVNVANFIQRLDALVILTLIIGVFFKMSIYCFAAAAITADLFKV 300 +D+ +R+ FP+ +T+ + VA+F++RLD + ++IG++FK+SI +A+ TA LFK+ Sbjct: 241 VDLTTRSQFPLLSTVQTIQVADFLERLDVFFMFAMVIGIYFKVSILFYASIISTATLFKI 300 Query: 301 KDYRRLVVPVGVIVLISSFISVENYTSHL 329 K RL P+G+IVL S N+ HL Sbjct: 301 KSPSRLAYPLGIIVLFLSITIASNFQEHL 329 >ref|NP_388254.1| gerKB [Bacillus subtilis] sp|P49940|GRKB_BACSU Spore germination protein KB pir||I39860 germination response protein gerKB - Bacillus subtilis dbj|BAA11256.1| GerKB [Bacillus subtilis] dbj|BAA09004.1| spore germination protein GerKB [Bacillus subtilis] emb|CAB12180.1| gerKB [Bacillus subtilis subsp. subtilis str. 168] Length = 373 Score = 230 bits (586), Expect = 5e-59, Method: Compositional matrix adjust. Identities = 121/332 (36%), Positives = 199/332 (59%) Query: 1 MKKEVIGPSQVFAMIVLFELGTAIVVPIGLESGHAVWLSILMALPGGVLLYLIYNDLYRQ 60 M+K I Q+F MI++FELG+++++ G +G W+++L+ G+ L+ +Y +Y+ Sbjct: 1 MEKARISIRQLFVMIIIFELGSSLLITPGSMAGRDAWIAVLLGCAIGLFLFYLYQGIYQC 60 Query: 61 FPNLIISGYTRKILGNYIGWPLSLLYIPVLMFNGSRNLREAGDLLISASYDRTPILIINS 120 +PN Y +LG + W S LYI + +R LR+ G++L++ +Y TPI+I+N+ Sbjct: 61 YPNSSPKEYMDDMLGTKLSWLFSFLYILYFAYIAARVLRDFGEMLLTFAYHDTPIIIVNA 120 Query: 121 IMVIAVMYILNKGIEVFARTAEIYFWIMMVMGLVCNGVVIIAGLVDFKNLFPLHYKDWVD 180 ++++ +Y + KGIEV AR AE+ F M ++G + ++I++G +D NL P+ Sbjct: 121 LLMVVSIYAVRKGIEVLARAAELLFGAMYLLGAIGLVLIIVSGTIDPHNLKPVLANGISP 180 Query: 181 ALTSAYPNIWIFPFGELVCFTTVLPFLKNSGISRKMGVTAIILSGFLLCFTHAIEISVLG 240 L S + PFGE+V F + P L + +KMG+ A+ +SG ++ T AI ISVL Sbjct: 181 VLHSVFTQTMYVPFGEVVLFVMIFPNLNDRKDVKKMGMIAMAISGLIVALTVAINISVLD 240 Query: 241 LDIYSRAAFPIYTTITLVNVANFIQRLDALVILTLIIGVFFKMSIYCFAAAAITADLFKV 300 +D+ R+ FP+ +TI + V F+ RLD +L LIIG FFK+S+Y +A T+ LFK Sbjct: 241 VDLTLRSQFPLLSTIQTIKVEEFLDRLDVFFMLALIIGGFFKVSLYLYATVVGTSTLFKE 300 Query: 301 KDYRRLVVPVGVIVLISSFISVENYTSHLNEG 332 K+ +L P+G+ +LI S N++ HLNEG Sbjct: 301 KNPSQLAYPMGLGILILSITIATNFSEHLNEG 332 >ref|NP_149186.1| Spore germination protein, GRKB [Clostridium acetobutylicum] gb|AAK76768.1| Spore germination protein, GRKB [Clostridium acetobutylicum] Length = 361 Score = 157 bits (397), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 99/319 (31%), Positives = 167/319 (52%), Gaps = 1/319 (0%) Query: 10 QVFAMIVLFELGTAIVVPIGLESGHAVWLSILMALPGGVLLYLIYNDLYRQFPNLIISGY 69 Q+F +I +FE+G+ + +G+E+ W+ +L+AL G+ + Y +L + FP + Sbjct: 8 QLFTLIFVFEVGSTPIFALGIEAEQNAWIVVLLALLIGLFILWSYLELQKAFPTKGLVEI 67 Query: 70 TRKILGNYIGWPLSLLYIPVLMFNGSRNLREAGDLLISASYDRTPILIINSIMVIAVMYI 129 ILG +G PL +LY ++ RNLRE +L+ TP+ II + +Y+ Sbjct: 68 FTNILGKPLGLPLCILYAMGFIWGCGRNLREFIELIAMTVIPHTPLWIILFFFIGLSLYV 127 Query: 130 LNKGIEVFARTAEIYFWIMMVMGLVCNGVVIIAGLVDFKNLFPLHYKDWVDALTSAYPNI 189 KG EVFART E F ++ ++ ++ I+G V FKNL P+ D + + + P + Sbjct: 128 SVKGFEVFARTTEFIFPTIIFFLIILFALISISGEVHFKNLTPI-LGDGLKPILKSLPGV 186 Query: 190 WIFPFGELVCFTTVLPFLKNSGISRKMGVTAIILSGFLLCFTHAIEISVLGLDIYSRAAF 249 FPFGE+ F ++ + +K A+ILSG LLCFT I I LG S A Sbjct: 187 VWFPFGEIYVFLMYWHYVNDINTIKKASFKAVILSGILLCFTTMINICTLGAKYTSIATI 246 Query: 250 PIYTTITLVNVANFIQRLDALVILTLIIGVFFKMSIYCFAAAAITADLFKVKDYRRLVVP 309 P+ T+ L+N+ + I LD + ++ + +G FFKM++Y A I +F +K + +++ Sbjct: 247 PLVETLRLINIGDIITHLDVIGVIIIFLGGFFKMTLYLNGIAIIINSVFSIKKSQLVLIL 306 Query: 310 VGVIVLISSFISVENYTSH 328 G+ ++ S ++ H Sbjct: 307 TGIFMMYFSIYFEPSFAYH 325 >ref|NP_348154.1| Spore germination protein GerKB [Clostridium acetobutylicum] pir||C97088 spore germination protein GerKB [imported] - Clostridium acetobutylicum gb|AAK79494.1| Spore germination protein GerKB [Clostridium acetobutylicum] Length = 365 Score = 126 bits (317), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 99/355 (27%), Positives = 173/355 (48%), Gaps = 1/355 (0%) Query: 6 IGPSQVFAMIVLFELGTAIVVPIGLESGHAVWLSILMALPGGVLLYLIYNDLYRQFPNLI 65 + Q+F + +F++G+ + +G+E+ W+ IL+AL G+L+ I +L+ FP+ Sbjct: 4 LSKHQLFTLTFIFQIGSTPLFALGIEAEQDAWIVILLALLIGLLMAWISTELFYIFPDKN 63 Query: 66 ISGYTRKILGNYIGWPLSLLYIPVLMFNGSRNLREAGDLLISASYDRTPILIINSIMVIA 125 + ILG +G P ++Y V ++ RNLRE +L++ S + II ++ Sbjct: 64 LIEIINIILGKKLGLPFCIVYAMVFIWACGRNLREFSELILLISLPHIHLWIIILSYILL 123 Query: 126 VMYILNKGIEVFARTAEIYFWIMMVMGLVCNGVVIIAGLVDFKNLFPLHYKDWVDALTSA 185 +Y L KG E R +E+ ++ + ++I +NL P+ + + + + Sbjct: 124 ALYTLFKGFETLCRLSELIVPPTIIFLISMFIIIIFFSSAHIRNLMPI-LGNGIKPVLKS 182 Query: 186 YPNIWIFPFGELVCFTTVLPFLKNSGISRKMGVTAIILSGFLLCFTHAIEISVLGLDIYS 245 P + FPFGE+ +L + RK A+I SG LLC + A+ IS LG S Sbjct: 183 LPGVIWFPFGEVFVLLLYWHYLNDKKSVRKTTFKALISSGLLLCISTAVTISELGAKYTS 242 Query: 246 RAAFPIYTTITLVNVANFIQRLDALVILTLIIGVFFKMSIYCFAAAAITADLFKVKDYRR 305 + P+ T+ L+N+ + RLD L I LI+G FFK+ IY + A +FK+ ++ Sbjct: 243 VSTIPLVETLRLINIVEVLNRLDILGIPFLILGGFFKICIYLNSIALTINSVFKINHFKL 302 Query: 306 LVVPVGVIVLISSFISVENYTSHLNEGKLFLKYFLPVICAVVPILLFLVHRIRKR 360 ++ GV +L S NY H +YF C P +L +++ I+ + Sbjct: 303 TLMLCGVFMLFFSIYFEPNYAYHQWLFPFDARYFCLFFCTFYPTMLLVIYIIKLK 357 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.332 0.147 0.452 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 528,886,606 Number of extensions: 21889353 Number of successful extensions: 69804 Number of sequences better than 1.0e-05: 39 Number of HSP's gapped: 69783 Number of HSP's successfully gapped: 39 Length of query: 367 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 240 Effective length of database: 412,420,005 Effective search space: 98980801200 Effective search space used: 98980801200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 126 (53.1 bits)