BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0054.0005.p (521 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAB61393.1| germination protein 526 e-148 ref|NP_388252.1| gerKA [Bacillus subtilis] >gi|7531270|sp|P49939... 486 e-136 pir||I39858 germination response gerKA protein - Bacillus subtil... 477 e-133 ref|NP_242800.1| spore germination protein KA [Bacillus halodura... 430 e-119 ref|NP_623914.1| germination protein [Thermoanaerobacter tengcon... 427 e-118 >gb|AAB61393.1| germination protein Length = 540 Score = 526 bits (1354), Expect = e-148, Method: Compositional matrix adjust. Identities = 260/510 (50%), Positives = 345/510 (67%), Gaps = 10/510 (1%) Query: 6 QTKEKKQ---SQTADSNTSSPLSGNLKDDLQHIRQGLGNSMDLIIRHFKL-INAALQVAV 61 ++K K Q QT + T S L L ++L I++ GNS D++IRHFK+ +N +L A+ Sbjct: 9 KSKNKLQPQADQTNNEQTVSSLKNLLSENLTLIKEKTGNSSDIVIRHFKIGVNNSLAAAI 68 Query: 62 IYMEGLTDKELVDDFISHAMSFQTVSEEGQKLS-TSQEVFNYIKEKALTPGKSQSLTNRG 120 +Y+EG+ D + + D++ Q++ ++ QK Q I E +T G N Sbjct: 69 VYIEGIVDNQAIQDYL-----LQSLMKDNQKNDLNDQNALELISEDIVTMGNVSFADNWN 123 Query: 121 GLFEALLSGNTLVLVEGMPEALSGDTSGGEVRPVSEAGTQVSIRGSKASFTETISVNISL 180 L +L+SG++L++V+G+ LS T GGE R ++E+ TQ+ +RG K +FTE+I N+++ Sbjct: 124 DLLSSLMSGDSLLIVDGINRVLSVSTQGGEKRAIAESTTQMVVRGPKGAFTESIGTNLAM 183 Query: 181 VRRRIKNPNLWVEPMKLGAVTQTNIAIMYIHGIAXXXXXXXXXXXXXXXXXDSILESGYI 240 VRR IK P+LW+E MK+G VT+T++ +MYIHGIA DSILESGY+ Sbjct: 184 VRRIIKTPDLWLESMKIGRVTKTDVTLMYIHGIANDKVVKEIRKRLKNIDIDSILESGYV 243 Query: 241 EQLIEGSGYSPFPTLYNTERPDTVAGNLLDGRIAVFVDGTPFVLIAPATFFMFFHTVEDY 300 EQLIE +PFPT+YNTERPD VAGNLL+GRIA+FVDGTPF LIAPA F FF + EDY Sbjct: 244 EQLIEDQTVTPFPTIYNTERPDVVAGNLLEGRIAIFVDGTPFGLIAPALFIQFFQSAEDY 303 Query: 301 YQRFDISTXXXXXXXXXXXXSMFGPAIFVAALNFHQEMIPTPLLINLASQREGVPFPAFV 360 Y RFDI+T S+ PA +VA FHQEM+PT L++ LA+QRE VPFPAFV Sbjct: 304 YARFDIATSIRLLRVFMFMISLIAPATYVAVTTFHQEMVPTTLIVALAAQRESVPFPAFV 363 Query: 361 EALMMEVTFEIIREAGIRLPSPIGQTVSIIGGLVLGQAAVQAGIVSPAMVIVVSITGISS 420 EA MEVTFEI+REAGIRLP IG VSI+G LV+GQAAVQAGIVSPAMVI+VSIT I+S Sbjct: 364 EAFFMEVTFEILREAGIRLPKAIGSAVSIVGALVIGQAAVQAGIVSPAMVIIVSITAIAS 423 Query: 421 FTTPAFNMALSVRLLRFVIVFVSAFMGLYGIAILALMLVAHLCGLRSLGVPYMSPLGPFD 480 F TP+F+MA+S RL+RF+ + +A G YGI ++ LM V HLC LRS GVPYM+PL PF Sbjct: 424 FATPSFDMAISARLIRFLFILGAATFGFYGIILILLMGVGHLCSLRSFGVPYMTPLAPFI 483 Query: 481 PQAQKDTLIRSPIHFMKIRPRLEHKSNVRK 510 P + +DTL+R P M+ RP+L + VR+ Sbjct: 484 PVSNEDTLLRLPWWTMRRRPKLISDNKVRQ 513 >ref|NP_388252.1| gerKA [Bacillus subtilis] sp|P49939|GRKA_BACSU Spore germination protein KA dbj|BAA09002.1| spore germination protein GerKA [Bacillus subtilis] emb|CAB12178.1| gerKA [Bacillus subtilis subsp. subtilis str. 168] Length = 544 Score = 486 bits (1252), Expect = e-136, Method: Compositional matrix adjust. Identities = 256/514 (49%), Positives = 324/514 (63%), Gaps = 14/514 (2%) Query: 7 TKEKKQSQTADSNTSSPLSGNLKDDLQHIRQGLGNSMDLIIRHFKL-INAALQVAVIYME 65 T E+ Q Q + +S +L ++ ++ +G+S D+IIR K+ + +AVIY+ Sbjct: 19 TDERNQEQQQEKERPVLISPSLAKNIAETKKEVGSSSDVIIREIKIGEQDHVHLAVIYIS 78 Query: 66 GLTDKELVDDFISHAMSFQTVSEEGQKLSTSQEVFNYIKEKALTPGKSQSLTNRGGLFEA 125 GL D + + + + E Q Q++ EK L G ++ + LF Sbjct: 79 GLVDNNTIHESLIDPL---VQDESIQNTHAIQQIL----EKTLPLGGVKAEKSWDKLFSE 131 Query: 126 LLSGNTLVLVEGMPEALSGDTSGGEVRPVSEAGTQVSIRGSKASFTETISVNISLVRRRI 185 L+ GN L+ +G EAL T GGE R + E TQVS RG + FTE++ NIS++RR I Sbjct: 132 LMLGNALIFADGHDEALICSTQGGEQRSIQEPSTQVSFRGPRQGFTESLQTNISMIRRYI 191 Query: 186 KNPNLWVEPMKLGAVTQTNIAIMYIHGIAXXXXXXXXXXXXXXXXXDSILESGYIEQLIE 245 KNPNLWVE MK G+VT T+IA+MYI GI DSILESGYIEQLIE Sbjct: 192 KNPNLWVEKMKKGSVTNTDIALMYIQGICDEKVLKEVKQRLEKIDIDSILESGYIEQLIE 251 Query: 246 GSGYSPFPTLYNTERPDTVAGNLLDGRIAVFVDGTPFVLIAPATFFMFFHTVEDYYQRFD 305 ++ FPT+Y+TERPD VAGNLL+GR A+ VDGTPFVLIAPA F FF +VEDYY RFD Sbjct: 252 DETFTTFPTMYHTERPDVVAGNLLEGRFAIIVDGTPFVLIAPALFVQFFQSVEDYYSRFD 311 Query: 306 ISTXXXXXXXXXXXXSMFGPAIFVAALNFHQEMIPTPLLINLASQREGVPFPAFVEALMM 365 I+T S+ PA++VAA FHQEMIPT LL+ +A+QRE VPFPA VEAL M Sbjct: 312 IATSIRILRVLVFFISLVAPAVYVAATTFHQEMIPTQLLVVIAAQREIVPFPAVVEALTM 371 Query: 366 EVTFEIIREAGIRLPSPIGQTVSIIGGLVLGQAAVQAGIVSPAMVIVVSITGISSFTTPA 425 EV FEI+REAG+RLP +G VSI+G LV+GQAAVQAGIVSPAMVI+V++T I+SF TPA Sbjct: 372 EVAFEILREAGVRLPRVVGSAVSIVGALVIGQAAVQAGIVSPAMVIIVALTAIASFATPA 431 Query: 426 FNMALSVRLLRFVIVFVSAFMGLYGIAILALMLVAHLCGLRSLGVPYMSPLGPFDPQAQK 485 F MA+S RL+RF+ + SA MG YG+ + +M+ HLC LRS GVPYMSPL PF Q K Sbjct: 432 FAMAISARLIRFIFIIASAVMGFYGLILGIIMMFVHLCSLRSFGVPYMSPLAPFSSQGVK 491 Query: 486 DTLIRSPIHFMKIRPRLEHKSNVRKKADKDRQGN 519 D L R P + RP E S K DK RQG Sbjct: 492 DALFRVPWWADEKRP--ESVS----KEDKVRQGK 519 >pir||I39858 germination response gerKA protein - Bacillus subtilis dbj|BAA11254.1| GerKA [Bacillus subtilis] Length = 546 Score = 477 bits (1227), Expect = e-133, Method: Compositional matrix adjust. Identities = 252/514 (49%), Positives = 321/514 (62%), Gaps = 14/514 (2%) Query: 7 TKEKKQSQTADSNTSSPLSGNLKDDLQHIRQGLGNSMDLIIRHFKL-INAALQVAVIYME 65 T E+ Q Q + +S +L ++ ++ +G+S D+IIR K+ + +AVIY+ Sbjct: 21 TDERNQEQQQEKERPVLISPSLAKNIAETKKEVGSSSDVIIREIKIGEQDHVHLAVIYIS 80 Query: 66 GLTDKELVDDFISHAMSFQTVSEEGQKLSTSQEVFNYIKEKALTPGKSQSLTNRGGLFEA 125 GL D + + + + E Q QE+ K G ++ + LF Sbjct: 81 GLVDNNTIHESLIDPL---VQDESIQNTHAIQEILR----KTAPLGGVKAEKSWDKLFSE 133 Query: 126 LLSGNTLVLVEGMPEALSGDTSGGEVRPVSEAGTQVSIRGSKASFTETISVNISLVRRRI 185 L+ GN L+ +G EAL T GGE R + E TQVS RG + FTE++ NIS++RR I Sbjct: 134 LMLGNALIFADGHDEALICSTQGGEQRSIQEPSTQVSFRGPRQGFTESLQTNISMIRRYI 193 Query: 186 KNPNLWVEPMKLGAVTQTNIAIMYIHGIAXXXXXXXXXXXXXXXXXDSILESGYIEQLIE 245 KNPNLWV M+ G+VT T+IA+MYI GI DSILESGYIEQLIE Sbjct: 194 KNPNLWVGKMEKGSVTNTDIALMYIQGICDEKVLKEVKQRLEKIDIDSILESGYIEQLIE 253 Query: 246 GSGYSPFPTLYNTERPDTVAGNLLDGRIAVFVDGTPFVLIAPATFFMFFHTVEDYYQRFD 305 ++ FPT+Y+TERPD VAGNLL+GR A+ VDGTPFVLIAPA F FF +VEDYY RFD Sbjct: 254 DETFTTFPTMYHTERPDVVAGNLLEGRFAIIVDGTPFVLIAPALFVQFFQSVEDYYSRFD 313 Query: 306 ISTXXXXXXXXXXXXSMFGPAIFVAALNFHQEMIPTPLLINLASQREGVPFPAFVEALMM 365 I+T S+ PA++VAA FHQEMIPT LL+ +A+QRE VPFPA VEAL M Sbjct: 314 IATSIRILRVLVFFISLVAPAVYVAATTFHQEMIPTQLLVVIAAQREIVPFPAVVEALTM 373 Query: 366 EVTFEIIREAGIRLPSPIGQTVSIIGGLVLGQAAVQAGIVSPAMVIVVSITGISSFTTPA 425 EV F+I+REAG+RLP +G VSI+G LV+GQAAVQAGIVSPAMVI+V++T I+SF TPA Sbjct: 374 EVAFKILREAGVRLPRVVGSAVSIVGALVIGQAAVQAGIVSPAMVIIVALTAIASFATPA 433 Query: 426 FNMALSVRLLRFVIVFVSAFMGLYGIAILALMLVAHLCGLRSLGVPYMSPLGPFDPQAQK 485 F MA+S RL+RF+ + SA MG YG+ + +M+ HLC LRS GVPYMSPL PF Q K Sbjct: 434 FAMAISARLIRFIFIIASAVMGFYGLILGIIMMFVHLCSLRSFGVPYMSPLAPFSSQGVK 493 Query: 486 DTLIRSPIHFMKIRPRLEHKSNVRKKADKDRQGN 519 D L R P + RP E S K DK RQG Sbjct: 494 DALFRVPWWADEKRP--ESVS----KEDKVRQGK 521 >ref|NP_242800.1| spore germination protein KA [Bacillus halodurans] pir||F83891 spore germination protein KA gerKA [imported] - Bacillus halodurans (strain C-125) dbj|BAB05653.1| spore germination protein KA [Bacillus halodurans] Length = 515 Score = 430 bits (1106), Expect = e-119, Method: Compositional matrix adjust. Identities = 213/477 (44%), Positives = 311/477 (65%), Gaps = 1/477 (0%) Query: 24 LSGNLKDDLQHIRQGLGNSMDLIIRHFKLINA-ALQVAVIYMEGLTDKELVDDFISHAMS 82 LS +L+++ + I++ LGNS DL+IR +I+ + +++ GL D++LV + I A Sbjct: 32 LSESLEENKKKIKELLGNSSDLVIRELMIIHEPEISGVAVFINGLADEQLVSECIVSATL 91 Query: 83 FQTVSEEGQKLSTSQEVFNYIKEKALTPGKSQSLTNRGGLFEALLSGNTLVLVEGMPEAL 142 + K T + + I+++ L+ + Q+ + +LLSGN++VL++G +AL Sbjct: 92 HPSKEAHYFKEDTKDKRLSIIEKQILSVARVQAYDLWNPIIHSLLSGNSIVLIDGFDKAL 151 Query: 143 SGDTSGGEVRPVSEAGTQVSIRGSKASFTETISVNISLVRRRIKNPNLWVEPMKLGAVTQ 202 S T G + R + E T+ ++RG K +F E++ N+SL+RRR+K+ L VE +LG T Sbjct: 152 SCSTPGSKWRSIEEPSTETTVRGPKDAFNESLQTNLSLIRRRVKSNRLRVENTELGETTH 211 Query: 203 TNIAIMYIHGIAXXXXXXXXXXXXXXXXXDSILESGYIEQLIEGSGYSPFPTLYNTERPD 262 T++AI+Y+ + ++ IE+ ++ +PFPT YNTERPD Sbjct: 212 TDVAIVYVDDLVDKKLLQEIKQRLNRVITHKVISEANIEEAMQDEMLTPFPTSYNTERPD 271 Query: 263 TVAGNLLDGRIAVFVDGTPFVLIAPATFFMFFHTVEDYYQRFDISTXXXXXXXXXXXXSM 322 VA L++G++A+ +DGTPF LI P F FF EDYYQR+D+ST S+ Sbjct: 272 MVAYALVEGKVALIIDGTPFALILPTDFAQFFKAAEDYYQRYDLSTFIRLLRYFAFTVSL 331 Query: 323 FGPAIFVAALNFHQEMIPTPLLINLASQREGVPFPAFVEALMMEVTFEIIREAGIRLPSP 382 GP+++VA + FHQ++IPT LLI LASQREG+PFPA VEAL+ME TFE++REAG+R+P Sbjct: 332 LGPSLYVALITFHQDLIPTTLLIRLASQREGIPFPALVEALIMEFTFELLREAGLRMPRA 391 Query: 383 IGQTVSIIGGLVLGQAAVQAGIVSPAMVIVVSITGISSFTTPAFNMALSVRLLRFVIVFV 442 IGQ VSI+G LV+GQAAVQAGIVS AMVIVV+ TGI+SFTTPA+N+A++ RLLRF I+ + Sbjct: 392 IGQAVSIVGALVIGQAAVQAGIVSAAMVIVVAATGIASFTTPAYNLAVAARLLRFFIMLL 451 Query: 443 SAFMGLYGIAILALMLVAHLCGLRSLGVPYMSPLGPFDPQAQKDTLIRSPIHFMKIR 499 +A GLYGI + +MLVAHL LRS+G+PY +P PF +AQKD L R P+ ++ R Sbjct: 452 AATFGLYGIILGLIMLVAHLSSLRSVGMPYFAPFAPFSMKAQKDGLFRLPLKWLDKR 508 >ref|NP_623914.1| germination protein [Thermoanaerobacter tengcongensis] gb|AAM25518.1| germination protein [Thermoanaerobacter tengcongensis] Length = 511 Score = 427 bits (1097), Expect = e-118, Method: Compositional matrix adjust. Identities = 214/495 (43%), Positives = 319/495 (64%), Gaps = 9/495 (1%) Query: 24 LSGNLKDDLQHIRQGLGNSMDLIIRHFKLINAALQVAVIYMEGLTDKELVDDFISHAMSF 83 L+ +LK++ L + D++ R+ K+ + +IY++GL DK LV +H + F Sbjct: 20 LTKDLKENTYIFNTLLAGNNDVVFRNIKIGRRKIPACLIYIDGLADKSLV----AHNVLF 75 Query: 84 QTVSEEGQKLS----TSQEVFNYIKEKALTPGKSQSLTNRGGLFEALLSGNTLVLVEGMP 139 + E QK+ T E F ++E +T G+ G + + LLSG T++L++G+ Sbjct: 76 PLMIET-QKIDENPGTGDEFFERVRELFVTIGEVNVKDKIGDVMDDLLSGETVLLIDGIK 134 Query: 140 EALSGDTSGGEVRPVSEAGTQVSIRGSKASFTETISVNISLVRRRIKNPNLWVEPMKLGA 199 +AL T G E R +SE+ T+ +RG K +F ET+ +N S++RRRIK+P+ +E MK+G Sbjct: 135 KALILSTKGWEGRNISESTTESYVRGPKEAFVETLRINTSMLRRRIKHPDFVIEDMKIGK 194 Query: 200 VTQTNIAIMYIHGIAXXXXXXXXXXXXXXXXXDSILESGYIEQLIEGSGYSPFPTLYNTE 259 ++T++AI YI IA D I++SGYIE+ IE + +SPFP + ++E Sbjct: 195 YSKTDVAIAYIKSIADDKVLQEVKKRLKKIQIDGIIDSGYIEEFIEDNPFSPFPQIAHSE 254 Query: 260 RPDTVAGNLLDGRIAVFVDGTPFVLIAPATFFMFFHTVEDYYQRFDISTXXXXXXXXXXX 319 +PD +A +L+G++A+ VDG+PFVL PA F FF EDYY+R+ +S+ Sbjct: 255 KPDRIAAEILEGKVAIIVDGSPFVLTVPAVFAQFFQAGEDYYERYFLSSALRVLRIIAMI 314 Query: 320 XSMFGPAIFVAALNFHQEMIPTPLLINLASQREGVPFPAFVEALMMEVTFEIIREAGIRL 379 ++ P+I++A FHQEMIPT L I++A+QREGVPFPA +EAL MEVTFEI+REAGIRL Sbjct: 315 TALLFPSIYIAETTFHQEMIPTQLAISIAAQREGVPFPALLEALFMEVTFEILREAGIRL 374 Query: 380 PSPIGQTVSIIGGLVLGQAAVQAGIVSPAMVIVVSITGISSFTTPAFNMALSVRLLRFVI 439 P +GQ VSI+GGLV+GQAAVQAG+VSPAMVIVV++TGI+SF PAFN+A++ RLLRF+I Sbjct: 375 PMQVGQAVSIVGGLVIGQAAVQAGLVSPAMVIVVAMTGIASFAIPAFNVAITFRLLRFII 434 Query: 440 VFVSAFMGLYGIAILALMLVAHLCGLRSLGVPYMSPLGPFDPQAQKDTLIRSPIHFMKIR 499 + + +G +GI ++ +M++AH+ L S GVPY+SP+ P Q D +R P +R Sbjct: 435 MIAAGTLGFFGIIMVIMMVLAHMASLESFGVPYLSPITPCRSQELTDVFMRVPWWAKILR 494 Query: 500 PRLEHKSNVRKKADK 514 PR + N+ ++ DK Sbjct: 495 PRTIQRKNIIRQEDK 509 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.136 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 674,605,152 Number of extensions: 25776159 Number of successful extensions: 72679 Number of sequences better than 1.0e-05: 89 Number of HSP's gapped: 72473 Number of HSP's successfully gapped: 89 Length of query: 521 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 391 Effective length of database: 404,274,708 Effective search space: 158071410828 Effective search space used: 158071410828 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)