BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0053.0005.p (488 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_359301.1| Dextransucrase (sucrose 6-glucosyltransferase) ... 382 e-104 gb|AAO21861.1| sucrose phosphorylase [Lactobacillus acidophilus] 379 e-104 ref|NP_964279.1| sucrose phosphorylase [Lactobacillus johnsonii ... 379 e-103 gb|AAO21868.1| sucrose phosphorylase [Lactobacillus acidophilus] 377 e-103 ref|ZP_00070311.1| COG0366: Glycosidases [Oenococcus oeni MCW] 376 e-103 >ref|NP_359301.1| Dextransucrase (sucrose 6-glucosyltransferase) [Streptococcus pneumoniae R6] pir||C98085 dextransucrase (EC 2.4.1.5) [imported] - Streptococcus pneumoniae (strain R6) gb|AAL00512.1| Dextransucrase (sucrose 6-glucosyltransferase) [Streptococcus pneumoniae R6] Length = 480 Score = 382 bits (980), Expect = e-104, Method: Compositional matrix adjust. Identities = 198/469 (42%), Positives = 286/469 (60%), Gaps = 10/469 (2%) Query: 2 VKNQVQLITYPDSLGGDLKSLHHALDVYFPDVFKGGVHILPPFPSSGDRGFAPLTYLEIE 61 ++N+ LITY DSLG +LK L+ L+ +F D GGVH+LP FPS+GDRGFAP+ Y E++ Sbjct: 3 IQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAI-GGVHLLPFFPSTGDRGFAPVDYDEVD 61 Query: 62 PAFGDWADIRAIGERHDVLVDLMVNHISRQSPYFQDVLQHGRQSRYADLFLTLDKIWADG 121 AFGDW D++ +GER+ ++ D M+NHISRQS Y++D + S + LFL DK W + Sbjct: 62 SAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKHEASEFKALFLNWDKFWPEN 121 Query: 122 QPVQADIDKMFLRRSKPYSTFTIQDTGEELQVWTTFGKNDPSEQIDLDIRSEKVKQLFIQ 181 +P Q+D+D ++ R+ + + + G +W TFG+ EQIDLD+ E + + Sbjct: 122 RPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGE----EQIDLDVTKEVTMEFIRK 177 Query: 182 FFTCFKENNIKIVRLDAVGYVIKKLGTSCFFVEPEIYGFLDWIKELADSLDIELLPEVHS 241 N ++RLDA Y +KKL T+ FFVEP+I+ LD ++++A ELLPE+H Sbjct: 178 TIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLDKVRDIAAEYGTELLPEIHE 237 Query: 242 HYSIQYKLAEHGCWIYDFILPYRILDTLLNKDSRGLREYLRTRPAKQFTMLDCHDGVPVK 301 HYSIQ+K+A+H ++YDF LP L TL + + L ++L+ P KQFT LD HDG+ V Sbjct: 238 HYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKWLKMSPMKQFTTLDTHDGIGV- 296 Query: 302 PDLDDLIDTKDAQKIVDICVERGANLSLILSDEHKAPDGFDVHQIRCSYYSVLNCDDDAY 361 D+ D++ ++ + + GAN+ S + D++QI +YYS L DD Y Sbjct: 297 VDVKDILTDEEIDYASNELYKVGANVKRKYSSAEY--NNLDIYQINSTYYSALGDDDVKY 354 Query: 362 LAARAIQFFAPGIPQVYYVGLLAGENDLERVRETGEGREINRRNYTLPEIGQSLEKEVVQ 421 AR IQ FAPGIPQVYYVGLLAG+NDL+ + ET EGR INR Y+ EI + +++ VV+ Sbjct: 355 FLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGRNINRHYYSNEEIAKEVQRPVVK 414 Query: 422 RLLKLIRFRNEYEAFN--GDFTVEEAAADEIRLSWKKGEFRCTLYINLD 468 LL L FRN EAF+ G +E A I + + + T + +D Sbjct: 415 ALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQNKDKSVTAVVEID 463 >gb|AAO21861.1| sucrose phosphorylase [Lactobacillus acidophilus] Length = 480 Score = 379 bits (973), Expect = e-104, Method: Compositional matrix adjust. Identities = 196/482 (40%), Positives = 291/482 (60%), Gaps = 14/482 (2%) Query: 2 VKNQVQLITYPDSLGGDLKSLHHALDVYFPDVFKGGVHILPPFPSSGDRGFAPLTYLEIE 61 ++N+ LITYPDSLG +LK L H +D YF GG+H+LP FPS+GDRGF+P Y +E Sbjct: 3 LQNKAILITYPDSLGHNLKDLDHVMDRYFNKTI-GGIHLLPFFPSNGDRGFSPTRYDVVE 61 Query: 62 PAFGDWADIRAIGERHDVLVDLMVNHISRQSPYFQDVLQHGRQSRYADLFLTLDKIWADG 121 P FG W D+ + +++ ++ D M+NH+S++S YF+D +S+Y+DLFL+ DK W G Sbjct: 62 PKFGSWEDVEKLSQKYYLMFDFMINHLSKKSSYFEDFEAKHDKSKYSDLFLSWDKFWPKG 121 Query: 122 QPVQADIDKMFLRRSK-PYSTFTIQDTGEELQVWTTFGKNDPSEQIDLDIRSEKVKQLFI 180 +P + DID ++ R+ K PY +D G ++W TFG +Q+DLD+R++ + Sbjct: 122 RPTKEDIDLIYKRKDKAPYQNIKFED-GTHEKMWNTFG----PDQMDLDVRTKTTQDFIK 176 Query: 181 QFFTCFKENNIKIVRLDAVGYVIKKLGTSCFFVEPEIYGFLDWIKELADSLDIELLPEVH 240 ++ ++RLDA Y IKKL T+ FFVEPEI+ L+ + + +LPE+H Sbjct: 177 HNLQNLSKHGASLIRLDAFAYAIKKLDTNDFFVEPEIWNLLEKVNDYLKDTPTTILPEIH 236 Query: 241 SHYSIQYKLAEHGCWIYDFILPYRILDTLLNKDSRGLREYLRTRPAKQFTMLDCHDGVPV 300 HY++ +K+AEHG +IYDF LP +L +L + +S L +L+ P KQFT LD HDG+ V Sbjct: 237 EHYTMPFKVAEHGYFIYDFALPMVLLYSLYSGNSTQLAAWLKKCPMKQFTTLDTHDGLGV 296 Query: 301 KPDLDDLIDTKDAQKIVDICVERGANLSLILSDEHKAPDGFDVHQIRCSYYSVLNCDDDA 360 D D++ + + GAN+ S D++QI +YYS L DD Sbjct: 297 -VDAKDILTDDQISYTTNELYKIGANVKKKYSSAEYH--NLDIYQINTTYYSALGNDDKK 353 Query: 361 YLAARAIQFFAPGIPQVYYVGLLAGENDLERVRETGEGREINRRNYTLPEIGQSLEKEVV 420 Y AR +Q FAPGIPQ+YYVGLLAGEND++ + +T EGR+INR Y L EI + +++ VV Sbjct: 354 YFIARLLQIFAPGIPQIYYVGLLAGENDIQLLEKTKEGRDINRHYYDLDEIAEQVQRPVV 413 Query: 421 QRLLKLIRFRNEYEAFN--GDFTVEEAAADEIRL--SWKKGEFRCTLYINLDTMRSEIEY 476 + L+KL+ FRN AF+ G VE + EI + S K G + Y++ + +++Y Sbjct: 414 KSLIKLLEFRNSVPAFDLEGSIKVETPSEHEIIVTRSNKAGTEVASTYVDFKNLDYQVKY 473 Query: 477 TD 478 D Sbjct: 474 ND 475 >ref|NP_964279.1| sucrose phosphorylase [Lactobacillus johnsonii NCC 533] gb|AAS08245.1| sucrose phosphorylase [Lactobacillus johnsonii NCC 533] Length = 480 Score = 379 bits (973), Expect = e-103, Method: Compositional matrix adjust. Identities = 209/482 (43%), Positives = 292/482 (60%), Gaps = 22/482 (4%) Query: 2 VKNQVQLITYPDSLGGDLKSLHHALDVYFPDVFKGGVHILPPFPSSGDRGFAPLTYLEIE 61 + N+V LITYPDSLG ++K L L GGVH+LP FPS+GDRGFAP Y ++ Sbjct: 3 ISNKVMLITYPDSLGKNIKDLDDVLHTDLKGAV-GGVHLLPFFPSTGDRGFAPTDYSMVD 61 Query: 62 PAFGDWADIRAIGERHDVLVDLMVNHISRQSPYFQDVLQHGRQSRYADLFLTLDKIWADG 121 P FGDW+D+ +GE + ++ D M+NHISRQS Y++D ++ QS YADLFL+ DK W Sbjct: 62 PQFGDWSDVEKLGEDYYLMFDFMINHISRQSKYYKDFQKNKDQSPYADLFLSWDKFWPKN 121 Query: 122 QPVQADIDKMFLRRSK-PYSTFTIQDTGEELQVWTTFGKNDPSEQIDLDIRSEKVKQLFI 180 +P Q DID ++ R+ + PY T D +E ++W TFG+ EQIDLD+R + KQ Sbjct: 122 RPTQKDIDLIYKRKDRAPYQEITFADGSKE-KLWNTFGE----EQIDLDVRKDVTKQFIK 176 Query: 181 QFFTCFKENNIKIVRLDAVGYVIKKLGTSCFFVEPEIYGFLDWIKELADSLDIE---LLP 237 Q ++ I+RLDA Y IKKL T+ FFVEPEI+ D +KE+ D + + +LP Sbjct: 177 QTLNNLIDHGSDIIRLDAFAYAIKKLDTNDFFVEPEIW---DLLKEVQDDISAKGALILP 233 Query: 238 EVHSHYSIQYKLAEHGCWIYDFILPYRILDTLLNKDSRGLREYLRTRPAKQFTMLDCHDG 297 E+H HYS+ +K+A HG +IYDF LP +L +L + S+ L +L+ P KQFT LD HDG Sbjct: 234 EIHEHYSMPFKIARHGYFIYDFALPMVVLYSLYSGKSQRLASWLKKSPMKQFTTLDTHDG 293 Query: 298 VPVKPDLDDLIDTKDAQKIVDICVERGANLSLILSDEHKAPDGFDVHQIRCSYYSVLNCD 357 + V D D++ + + + GAN+ S D++QI ++YS L D Sbjct: 294 IGV-VDARDILTPNEIGYTSNELYKVGANVKKKYSSAEYH--NLDIYQINSTFYSALGDD 350 Query: 358 DDAYLAARAIQFFAPGIPQVYYVGLLAGENDLERVRETGEGREINRRNYTLPEIGQSLEK 417 D Y AR +Q FAPGIPQVYYVG+LAG+ND+E + +T EGR INR Y+ EI + +++ Sbjct: 351 DKKYFMARLLQVFAPGIPQVYYVGMLAGKNDIELLEKTKEGRNINRHYYSREEIHEEVQR 410 Query: 418 EVVQRLLKLIRFRNEYEAF--NGDFTVEEAAADE---IRLSWKKGEFRCTLYINLDTMRS 472 VV L+ L+RFRN AF NG V +E IRL+ K + L NL+T+ Sbjct: 411 PVVSSLINLLRFRNNEAAFDLNGSIEVTTPTENEIHIIRLNQDKTR-KAELRANLETLAY 469 Query: 473 EI 474 ++ Sbjct: 470 KV 471 >gb|AAO21868.1| sucrose phosphorylase [Lactobacillus acidophilus] Length = 480 Score = 377 bits (968), Expect = e-103, Method: Compositional matrix adjust. Identities = 194/449 (43%), Positives = 278/449 (61%), Gaps = 10/449 (2%) Query: 2 VKNQVQLITYPDSLGGDLKSLHHALDVYFPDVFKGGVHILPPFPSSGDRGFAPLTYLEIE 61 ++N+V LITYPDSLG +LK L L GG+H+LP FPS+GDRGFAP Y E++ Sbjct: 3 IENKVMLITYPDSLGKNLKELDEILSEDLKGAV-GGIHLLPFFPSTGDRGFAPTGYTEVD 61 Query: 62 PAFGDWADIRAIGERHDVLVDLMVNHISRQSPYFQDVLQHGRQSRYADLFLTLDKIWADG 121 P FGDW+DI IG+++ ++ D M+NHISRQS +++D Q +S+YADLFL+ DK W +G Sbjct: 62 PKFGDWSDIEKIGKKYYLMFDFMINHISRQSKFYKDFKQKKDKSKYADLFLSWDKFWPEG 121 Query: 122 QPVQADIDKMFLRRSK-PYSTFTIQDTGEELQVWTTFGKNDPSEQIDLDIRSEKVKQLFI 180 +P + DID ++ R+ + PY T D +E ++W TFG+ EQID+D+R E ++ Sbjct: 122 RPTRKDIDLIYKRKDRAPYQEITFTDGSKE-KLWNTFGE----EQIDMDVRKEVTQKFIK 176 Query: 181 QFFTCFKENNIKIVRLDAVGYVIKKLGTSCFFVEPEIYGFLDWIKELADSLDIELLPEVH 240 ++ I+RLDA Y +KKL T+ FFVEPEI+ L +++ +LPE+H Sbjct: 177 DTLRALIDHGADIIRLDAFAYAVKKLDTNDFFVEPEIWDLLKQVQDDISDKGAMILPEIH 236 Query: 241 SHYSIQYKLAEHGCWIYDFILPYRILDTLLNKDSRGLREYLRTRPAKQFTMLDCHDGVPV 300 HYS+ +K+++HG +IYDF LP L +L + S L ++L+ P KQFT LD HDG+ V Sbjct: 237 EHYSMPFKISKHGYYIYDFALPMVTLYSLYSGKSNRLADWLKKCPMKQFTTLDTHDGIGV 296 Query: 301 KPDLDDLIDTKDAQKIVDICVERGANLSLILSDEHKAPDGFDVHQIRCSYYSVLNCDDDA 360 D D++ + + + + GAN+ S D++QI +YYS L DD Sbjct: 297 -VDARDILSPDEIKYTSNELYKVGANVKKKYSSAEYH--NLDIYQINTTYYSALGNDDKK 353 Query: 361 YLAARAIQFFAPGIPQVYYVGLLAGENDLERVRETGEGREINRRNYTLPEIGQSLEKEVV 420 Y AR IQ FAPGIPQVYYVG+LAG+ND+E + +T EGR INR Y E+ + ++ +V Sbjct: 354 YFIARLIQMFAPGIPQVYYVGMLAGKNDIELLEKTKEGRNINRHYYGREEVAEETKRPLV 413 Query: 421 QRLLKLIRFRNEYEAFNGDFTVEEAAADE 449 LLKL FRN AF+ D ++E +E Sbjct: 414 AALLKLFNFRNNEAAFDLDGSIEITTPNE 442 >ref|ZP_00070311.1| COG0366: Glycosidases [Oenococcus oeni MCW] Length = 489 Score = 376 bits (966), Expect = e-103, Method: Compositional matrix adjust. Identities = 193/454 (42%), Positives = 282/454 (62%), Gaps = 12/454 (2%) Query: 2 VKNQVQLITYPDSLGGDLKSLHHALDVYFPDVFKGGVHILPPFPSSGDRGFAPLTYLEIE 61 VKN+ LITY DS+G ++K L + LD Y D GGVH+LP FPS+GDRGFAP Y + Sbjct: 3 VKNKAMLITYSDSMGKNIKELQYILDKYIGDAI-GGVHLLPFFPSTGDRGFAPSDYTRVN 61 Query: 62 PAFGDWADIRAIGERHDVLVDLMVNHISRQSPYFQDVLQHGRQSRYADLFLTLDKIWADG 121 P FGDW D+ +G+++ ++ D M+NHISR+S +QD + S Y D F+ +K W G Sbjct: 62 PDFGDWEDVEELGKKYYLMFDFMINHISRESIMYQDFKEKKDASSYKDFFIRWEKFWPKG 121 Query: 122 QPVQADIDKMFLRRSK-PYSTFTIQDTGEELQVWTTFGKNDPSEQIDLDIRSEKVKQLFI 180 +P +ADID ++ R+ K P T D +E +W TFG EQID++++S+ ++ F Sbjct: 122 RPTKADIDLIYKRKDKAPIQGITFADGSQE-HLWNTFG----DEQIDINVKSKVAQEFFK 176 Query: 181 QFFTCFKENNIKIVRLDAVGYVIKKLGTSCFFVEPEIYGFLDWIKELADSLDIELLPEVH 240 ++ ++RLDA Y IKK+ T+ FF+EPEI+ L+ ++++ D L E+LPE++ Sbjct: 177 DTLQSMVKHGADLIRLDAFAYAIKKIDTNDFFIEPEIWDLLESVRKILDPLHAEILPEIY 236 Query: 241 SHYSIQYKLAEHGCWIYDFILPYRILDTLLNKDSRGLREYLRTRPAKQFTMLDCHDGVPV 300 HY+I K+ E+G + YDF+LP IL TL + + + L ++L+ P KQFT LD HDG+ V Sbjct: 237 EHYTIPAKINEYGYFTYDFVLPLVILYTLYSGNPKQLAKWLKMSPKKQFTTLDTHDGIGV 296 Query: 301 KPDLDDLIDTKDAQKIVDICVERGANLSLILSDEHKAPDGFDVHQIRCSYYSVLNCDDDA 360 D D++ ++ + GAN+ S A + D++QI +YYS L DD A Sbjct: 297 -VDARDILTDEEIDYTSSELYKVGANVKRTYS--SAAYNNLDIYQINSTYYSALGNDDKA 353 Query: 361 YLAARAIQFFAPGIPQVYYVGLLAGENDLERVRETGEGREINRRNYTLPEIGQSLEKEVV 420 YL ARAIQ FAPGIPQ+YY GLLAGENDL+ + +T EGR INR Y+ E+ +++ +V Sbjct: 354 YLLARAIQIFAPGIPQIYYAGLLAGENDLDLLEKTKEGRNINRHYYSEEEVANEVQRPIV 413 Query: 421 QRLLKLIRFRNEYEAFN--GDFTVEEAAADEIRL 452 LLKL+ +RN AF+ GD V +EI++ Sbjct: 414 ACLLKLLAWRNRSAAFDLQGDIQVSATDKNEIKI 447 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.141 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 782,840,894 Number of extensions: 34692881 Number of successful extensions: 69315 Number of sequences better than 1.0e-05: 202 Number of HSP's gapped: 69132 Number of HSP's successfully gapped: 202 Length of query: 488 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 358 Effective length of database: 404,274,708 Effective search space: 144730345464 Effective search space used: 144730345464 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)