BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0045.0008.p (182 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_694302.1| transcriptional regulator [Oceanobacillus iheye... 171 6e-42 ref|NP_826080.1| putative TetR-family transcriptional regulator ... 84 2e-15 ref|ZP_00187178.1| hypothetical protein Rxyl185301 [Rubrobacter ... 70 2e-11 ref|NP_628801.1| putative tetR-family transcriptional regulator ... 70 3e-11 ref|NP_228836.1| transcriptional regulator, TetR family [Thermot... 61 1e-08 >ref|NP_694302.1| transcriptional regulator [Oceanobacillus iheyensis HTE831] dbj|BAC15336.1| transcriptional regulator (TetR-family) [Oceanobacillus iheyensis HTE831] Length = 171 Score = 171 bits (433), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 83/173 (47%), Positives = 120/173 (69%), Gaps = 4/173 (2%) Query: 8 VRTKEEAASVNRREQILEAAVVVFAEHGYFKATTAQVAEKVGISQPYIFKLFKNKEELFV 67 VR+ +E NR+E+ILEA + VFA+ GY TTA VAEK GISQPY+F+ FK KE+LF+ Sbjct: 2 VRSSKE----NRKEEILEAGLEVFAKRGYHNTTTAHVAEKAGISQPYVFRFFKTKEKLFI 57 Query: 68 AALDRAFERTIRTFSAVEASGDTLLEESIRVYEQMMETHPSEIILQVQALGIRDEAIRRS 127 AALDRAFER + F V +S + L+++ I YE++ E HP+EI LQV + + +EAIR + Sbjct: 58 AALDRAFERILLAFKNVHSSPEQLVDKMIEAYEELSELHPNEIALQVIGISVTEEAIRNA 117 Query: 128 MQTGMNKVTQLMENKFTAAGVAHPEVEVSTFMANGMLCHIAMALEMPELKPKH 180 + G++ + +F AG+ + E EV+TF+A GMLC+I+ L++PEL K+ Sbjct: 118 TKKGLSNIQNYTLERFKDAGIENVESEVTTFLARGMLCNISYFLDLPELIAKN 170 >ref|NP_826080.1| putative TetR-family transcriptional regulator [Streptomyces avermitilis MA-4680] dbj|BAC72615.1| putative TetR-family transcriptional regulator [Streptomyces avermitilis MA-4680] Length = 189 Score = 83.6 bits (205), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 51/171 (29%), Positives = 91/171 (53%), Gaps = 17/171 (9%) Query: 19 RREQILEAAVVVFAEHGYFKATTAQVAEKVGISQPYIFKLFKNKEELFVAALDRAFERTI 78 RRE ++ AA+ F GY+ +T +A++VG+SQPY+F+LF +K+ +F+AA +R E T Sbjct: 9 RRESVIRAAMSEFGRGGYYGTSTEAIAKRVGVSQPYLFRLFPSKKAIFLAAAERCMEDTR 68 Query: 79 RTF--SAVEASGDTLLEESIRVYEQMMETHPSEIILQVQ-------ALGIRDEAIRRSMQ 129 RTF +A G+ L Y +++ P ++++Q+Q A D + +++ Sbjct: 69 RTFEEAAEGLRGEEALHSMANAYTRVIAERPEQLLMQMQTYVASAAAEEAGDHELAEAVR 128 Query: 130 TGMNKVTQLMENKFTAAGVAHPEVEVSTFMANGMLCHIAMALEMPELKPKH 180 G ++ + + + E +TFMA GML + +A+ P P+H Sbjct: 129 AGWMRLWDTVHLP-----LGGDKDETTTFMAYGMLVNCLVAMGFP---PEH 171 >ref|ZP_00187178.1| hypothetical protein Rxyl185301 [Rubrobacter xylanophilus DSM 9941] Length = 180 Score = 70.5 bits (171), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 39/98 (39%), Positives = 59/98 (60%), Gaps = 2/98 (2%) Query: 19 RREQILEAAVVVFAEHGYFKATTAQVAEKVGISQPYIFKLFKNKEELFVAALDRAFERTI 78 RRE +L+AA+V FA +G TT +AE+VG+SQPY+ +LF +K+ LF+ A R F+R + Sbjct: 6 RREMVLDAAIVEFASYGLHGVTTDTIAERVGVSQPYVIRLFGSKKGLFLEAGQRVFDRIM 65 Query: 79 RTF-SAVEASGDTLLEESIRVYEQMMETHPSEIILQVQ 115 R AVE + R Y +M T E+++ +Q Sbjct: 66 RALREAVERQPQEPMRALQRAYVGLM-TQREELLMLIQ 102 >ref|NP_628801.1| putative tetR-family transcriptional regulator [Streptomyces coelicolor A3(2)] emb|CAB77414.1| putative tetR-family transcriptional regulator [Streptomyces coelicolor A3(2)] Length = 197 Score = 69.7 bits (169), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 47/171 (27%), Positives = 77/171 (45%), Gaps = 17/171 (9%) Query: 19 RREQILEAAVVVFAEHGYFKATTAQVAEKVGISQPYIFKLFKNKEELFVAALDRAFERTI 78 RRE ++ AA FA GY +T +A++VG+SQPY+F+LF K+ +F+A +R T Sbjct: 9 RRESVIRAATSEFARGGYHGTSTETIAKRVGVSQPYLFRLFPGKKAIFLAVAERCLRDTR 68 Query: 79 RTFSAVEAS--GDTLLEESIRVYEQMMETHPSEIILQVQ-------ALGIRDEAIRRSMQ 129 F G+ L Y Q++ P ++ +Q+Q A D ++ Sbjct: 69 EVFEKASEGLHGEEALHAMAEAYSQLIADQPEKLQMQLQVYVTVAAAEAAGDHEFGEMVR 128 Query: 130 TGMNKVTQLMENKFTAAGVAHPEVEVSTFMANGMLCHIAMALEMPELKPKH 180 G ++ + A E +TF+A GML + + + P P H Sbjct: 129 KGWLEIWDTVHLPLGA-----DIGETTTFLAYGMLINTLVGMGFP---PGH 171 >ref|NP_228836.1| transcriptional regulator, TetR family [Thermotoga maritima] pir||E72303 transcription regulator, TetR family - Thermotoga maritima (strain MSB8) gb|AAD36107.1| transcriptional regulator, TetR family [Thermotoga maritima] Length = 200 Score = 60.8 bits (146), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 39/123 (31%), Positives = 68/123 (55%), Gaps = 6/123 (4%) Query: 17 VNRREQILEAAVVVFAEHGYFKATTAQVAEKVGISQPYIFKLFKNKEELFVAALDRAFER 76 +++R+ IL+AAV VF + GY +ATT ++AEK G+++ IF FKNKEEL+ A E+ Sbjct: 2 LSKRDAILKAAVEVFGKKGYDRATTDEIAEKAGVAKGLIFHYFKNKEELYYQAYMSVTEK 61 Query: 77 TIRTFS--AVEASGDTLLEESIRVYEQMME---THPSEIILQVQALGIRDEAIRRSMQTG 131 + F ++ + + R E+ +E +HP E + + + DE +R+ + Sbjct: 62 LQKEFENFLMKNRNRDIFDFMERWIEKKLEYSASHPEEADFLITLVSV-DEGLRKRILLD 120 Query: 132 MNK 134 + K Sbjct: 121 LEK 123 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.130 0.349 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 218,412,280 Number of extensions: 7161275 Number of successful extensions: 26911 Number of sequences better than 1.0e-05: 54 Number of HSP's gapped: 26978 Number of HSP's successfully gapped: 54 Length of query: 182 Length of database: 757,237,578 Length adjustment: 118 Effective length of query: 64 Effective length of database: 436,855,896 Effective search space: 27958777344 Effective search space used: 27958777344 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 122 (51.6 bits)