BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0045.0003.p (180 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAO65794.1| putative monensin cluster negative regulator; Mon... 140 1e-32 ref|NP_629129.1| putative tetR-family transcriptional regulator ... 109 3e-23 ref|NP_824466.1| putative TetR-family transcriptional regulator ... 103 2e-21 ref|NP_962645.1| hypothetical protein MAP3711c [Mycobacterium av... 98 7e-20 ref|NP_826973.1| putative TetR-family transcriptional regulator ... 69 5e-11 >gb|AAO65794.1| putative monensin cluster negative regulator; MonRII [Streptomyces cinnamonensis] Length = 191 Score = 140 bits (353), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 72/162 (44%), Positives = 105/162 (64%) Query: 2 ASIQLHALQLFRQYGYKATTVEQIAEAAEISYSTFFRYFSTKEDVLLIDNYDPLLVASFE 61 A+IQ A++LFR+ GY ATT+EQIAEAAE++ ST FRYF+TK+D++ +YD + Sbjct: 14 AAIQREAVRLFREQGYTATTIEQIAEAAEVAPSTVFRYFATKQDLVFSHDYDLPFAMMVQ 73 Query: 62 SQPAHLTPLMALRNAMLSGAKEMTPQELSTMRERNELVMSVPELRAAMINNMVNMMDLII 121 +Q LTP+ A R A+ S ++++ QEL+ RER L++S PEL A + N+ M ++ Sbjct: 74 AQSPDLTPIQAERQAIRSMLQDISEQELALQRERFVLILSEPELWGASLGNIGQTMQIMS 133 Query: 122 ELTCRRTGREREDLAVRAFAGAVIGVNISVMQYYAEHPEADF 163 E +R GR+ D AVRA+ GAV GV + V +A P+ DF Sbjct: 134 EQVAKRAGRDPRDPAVRAYTGAVFGVMLQVSMDWANDPDMDF 175 >ref|NP_629129.1| putative tetR-family transcriptional regulator [Streptomyces coelicolor A3(2)] emb|CAD30962.1| putative tetR-family transcriptional regulator [Streptomyces coelicolor A3(2)] Length = 235 Score = 109 bits (272), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 59/156 (37%), Positives = 90/156 (57%) Query: 3 SIQLHALQLFRQYGYKATTVEQIAEAAEISYSTFFRYFSTKEDVLLIDNYDPLLVASFES 62 +I+ L RQ GY+ATTVEQIAE AE+S ST RYF T+ED++L D YDP++ A + Sbjct: 29 AIRAATYGLIRQQGYEATTVEQIAERAEVSPSTVLRYFPTREDIVLTDEYDPVMAAELAA 88 Query: 63 QPAHLTPLMALRNAMLSGAKEMTPQELSTMRERNELVMSVPELRAAMINNMVNMMDLIIE 122 +PA +LR+ + +E +R R L+ VP +RA M+ NM + ++ Sbjct: 89 RPAGEPWSDSLRHVLRKALGLGAGEEAELIRLRTRLLAEVPAVRARMLENMSDTGRMLAR 148 Query: 123 LTCRRTGREREDLAVRAFAGAVIGVNISVMQYYAEH 158 RTG + + L VR + +++G + V +Y+AEH Sbjct: 149 AIADRTGLDPDGLEVRIVSMSLVGGLMEVSRYWAEH 184 >ref|NP_824466.1| putative TetR-family transcriptional regulator [Streptomyces avermitilis MA-4680] dbj|BAC71001.1| putative TetR-family transcriptional regulator [Streptomyces avermitilis MA-4680] Length = 220 Score = 103 bits (256), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 57/147 (38%), Positives = 86/147 (58%) Query: 11 LFRQYGYKATTVEQIAEAAEISYSTFFRYFSTKEDVLLIDNYDPLLVASFESQPAHLTPL 70 L ++ GY ATT+EQIAE AE+S ST FRYF TKED++L D YDP++ ++PA Sbjct: 37 LIQEQGYDATTIEQIAERAEVSPSTVFRYFPTKEDIVLTDEYDPVMEKELTARPADEPWT 96 Query: 71 MALRNAMLSGAKEMTPQELSTMRERNELVMSVPELRAAMINNMVNMMDLIIELTCRRTGR 130 +LR A+ +E R R +L++ VP +R+ M+ +M ++ + RTGR Sbjct: 97 ESLRYAVHQAIGFGFTEEPHVSRLRTQLMVDVPAVRSRMMESMSVTGRMLCRVIAARTGR 156 Query: 131 EREDLAVRAFAGAVIGVNISVMQYYAE 157 + + L VR FA ++IG + Y+AE Sbjct: 157 DPDSLEVRVFAMSLIGGLMETSLYWAE 183 >ref|NP_962645.1| hypothetical protein MAP3711c [Mycobacterium avium subsp. paratuberculosis str. k10] gb|AAS06261.1| hypothetical protein MAP3711c [Mycobacterium avium subsp. paratuberculosis str. k10] Length = 200 Score = 98.2 bits (243), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 63/158 (39%), Positives = 91/158 (57%) Query: 3 SIQLHALQLFRQYGYKATTVEQIAEAAEISYSTFFRYFSTKEDVLLIDNYDPLLVASFES 62 +I+ A +L Q GY ATTVEQIAEAA++S STFFRYF +KE +LL D+ DPL++ + ++ Sbjct: 27 AIRREAFRLIEQNGYAATTVEQIAEAADVSPSTFFRYFGSKESLLLADDLDPLILDALKA 86 Query: 63 QPAHLTPLMALRNAMLSGAKEMTPQELSTMRERNELVMSVPELRAAMINNMVNMMDLIIE 122 QP L+ A R A + ++ +L R L+ S+PEL+AAM + + + E Sbjct: 87 QPRELSLPQAFRRAYATVLSQLPDDQLEFESTRQRLMFSIPELKAAMYDEYYRTVTVAAE 146 Query: 123 LTCRRTGREREDLAVRAFAGAVIGVNISVMQYYAEHPE 160 R GR +D VR FAGA+ G ++ PE Sbjct: 147 AIAHRIGRAADDFEVRVFAGALTGAMMAAFDSAPTTPE 184 >ref|NP_826973.1| putative TetR-family transcriptional regulator [Streptomyces avermitilis MA-4680] dbj|BAC73508.1| putative TetR-family transcriptional regulator [Streptomyces avermitilis MA-4680] Length = 226 Score = 68.9 bits (167), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 54/166 (32%), Positives = 82/166 (49%), Gaps = 24/166 (14%) Query: 8 ALQLFRQYGYKATTVEQIAEAAEISYSTFFRYFSTKEDVLLIDNY--DPLLVASFESQPA 65 AL+LF GY+ TTV++IAEA ++S TFFRYF+ KE+ + + VA+ +P Sbjct: 25 ALELFLAQGYERTTVDEIAEAVDVSQRTFFRYFAGKEEAAFLTQRIAESHFVAAVRERPP 84 Query: 66 HLTPLMALRNAMLSGA-------KEMTPQELSTMRERNELVMSVPELRAAMINNMVNMMD 118 H PL ALR A+L +++ P EL MR +L+ S P L A + + + + Sbjct: 85 HEAPLEALRRAVLESWETIGEAIEQVVPVELH-MRT-YQLIESTPALLAVHLRHSAELEE 142 Query: 119 LIIELTCRRTGRE-----REDLAVRAFAGAV--------IGVNISV 151 + + R G + R +AV F G + G N+SV Sbjct: 143 ELARIIAGREGLDVDADPRPRVAVAVFGGVIRVTGRLWGAGGNLSV 188 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.133 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 202,007,245 Number of extensions: 6652488 Number of successful extensions: 19751 Number of sequences better than 1.0e-05: 30 Number of HSP's gapped: 19747 Number of HSP's successfully gapped: 30 Length of query: 180 Length of database: 757,237,578 Length adjustment: 118 Effective length of query: 62 Effective length of database: 436,855,896 Effective search space: 27085065552 Effective search space used: 27085065552 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 122 (51.6 bits)