BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0005.p (455 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_442746.1| unknown protein [Synechocystis sp. PCC 6803] >g... 358 2e-97 gb|EAK35714.1| unknown [environmental sequence] 331 2e-89 ref|NP_384801.1| ALPHA-GLUCOSIDES-BINDING PERIPLASMIC ABC TRANSP... 320 3e-86 gb|AAD12050.1| AglE [Sinorhizobium meliloti] 320 4e-86 ref|NP_105832.1| alpha-glucosides ABC transporter, substrate-bin... 320 5e-86 >ref|NP_442746.1| unknown protein [Synechocystis sp. PCC 6803] pir||S75970 hypothetical protein - Synechocystis sp. (strain PCC 6803) Length = 421 Score = 358 bits (918), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 173/403 (42%), Positives = 251/403 (62%), Gaps = 8/403 (1%) Query: 52 MKGEYKGTKVTMFGPFVDADQVKFESSIKEFEAKTGIDIQYEGSKEFEATINIRVDGGNA 111 + G+ +VT+ G + Q K E ++ F TGI++ YEG F T+ IRVD G A Sbjct: 24 VNGDEDRKQVTILGVMIGEQQEKIEQALAPFTEATGIEVVYEGVDTFATTLPIRVDSGRA 83 Query: 112 PDIADFPQPGLLASIAKTGKVVDLTGVLDQEKLKANYNKSWLDMSTMDGKDGKIMAGIWN 171 PD+A FPQPGL+A A+ GK+V L +L E++ Y+++WLD++ +DG + G+W Sbjct: 84 PDLAMFPQPGLMADFAREGKLVPLGEILTPEEMTEAYDQAWLDLAAVDGT----VYGVWY 139 Query: 172 RSNVKSLVWYPKKQFDAAGYTVPETWDDMMALTEQIAKDGDPAWTIGIESGAATGWAATD 231 R++VKSLVW+ ++F A GY VP TW++MMAL++++ G W +GIESG ATGW TD Sbjct: 140 RASVKSLVWFNPQEFAANGYEVPGTWEEMMALSQRLIDKGKTPWCLGIESGNATGWVGTD 199 Query: 232 WIENIMLRTTTPENYDKWVKGELPFTSPEVKNAVEVMSKIWLNKDYVYGGTKSIVTTAFG 291 W+E+IMLRT +P YD+WV ++PF V+NA+++ +I N+ +YGG ++T FG Sbjct: 200 WVEDIMLRTASPATYDQWVAHDIPFNDRRVENALDIFGEITQNEKMIYGGKVGALSTPFG 259 Query: 292 DAPKPMFENPPKAWFNLMGNFITSFFPETAKVDQD-YDWFYLPPIDPQYGKPVLVAGDIY 350 D+ +F +PP + + GNFI +F P A VD D D F LPPI+ +YG P+LVAGDI+ Sbjct: 260 DSILGLFTDPPHCYLHRQGNFIAAFLP--ADVDDDQVDIFPLPPIEEEYGLPILVAGDIF 317 Query: 351 AMFNDRPEVRAVMEFFTTGESIKTWVQSGGVIAPMNDASLDWYTSESDRRMAKLVQDAST 410 AMFND PE R +M + + + G I+P + L+ Y R+ A+++ A Sbjct: 318 AMFNDTPEARQLMAYLASSRPHEVAATLGAYISPHKNIDLNLYPDRLTRKQAEILNKAEV 377 Query: 411 LRFDGSDLMPGKVGAGTFWKGMTDYVSGTATLDKALEQIQSGW 453 +RFD SD+MPG VG GTFW GM DY+ G A + L I+ W Sbjct: 378 IRFDASDMMPGAVGTGTFWSGMVDYIGG-ADGTQVLNTIERSW 419 >gb|EAK35714.1| unknown [environmental sequence] Length = 436 Score = 331 bits (848), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 175/404 (43%), Positives = 244/404 (60%), Gaps = 6/404 (1%) Query: 55 EYKGTKVTMFGPFVDADQVKFESSIKEFEAKTGIDIQYEGSKEFEATINIRVDGGNAPDI 114 + G KV +FGP++ F + FE TG ++Y GS EFE INI GN D+ Sbjct: 32 DLSGQKVVVFGPWMAPQDDDFRDVVSIFEKATGATVEYGGSDEFEQIINIDCKAGNPADV 91 Query: 115 ADFPQPGLLASIAKTGKVVDLTGVLDQEKLKANY--NKSWLDMSTMDGKDGK-IMAGIWN 171 A FPQPGL A+IA TG + + Q L NY +SW+D+ST ++GK GI+ Sbjct: 92 AVFPQPGLAANIAATGCLSPQGDDVKQMVLD-NYAAGQSWVDLSTYADENGKDQFYGIFY 150 Query: 172 RSNVKSLVWYPKKQFDAAGYTVPETWDDMMALTEQIAKDGDPAWTIGIESGAATGWAATD 231 R +VKSLVWY F+ GY +P+T +++++LTE++A DG+ W IG+ SG ATGW ATD Sbjct: 151 RVSVKSLVWYSPDNFEDNGYEIPQTMEELVSLTEKMASDGNTPWCIGLGSGGATGWPATD 210 Query: 232 WIENIMLRTTTPENYDKWVKGELPFTSPEVKNAVEVMSKIWLNKDYVYGGTKSIVTTAFG 291 W+E+IMLRT +P+ YD WV E+PF P V A++V LN YV GGTK++ TT F Sbjct: 211 WMEDIMLRTHSPDVYDMWVSNEMPFNDPRVIEAMDVFGSFALNDKYVSGGTKAVATTDFR 270 Query: 292 DAPKPMFENPPKAWFNLMGNFITSFFPETAKVDQDYDWFYLPPIDPQ-YGKPVLVAGDIY 350 D+PK +F +PPK + +FI +FFPE K +D D+FY P + G+PVL AG + Sbjct: 271 DSPKGLFTSPPKCMMHRQASFIPAFFPEGTKAGEDVDFFYFPAFSSKDLGRPVLGAGTVL 330 Query: 351 AMFNDRPEVRAVMEFFTTGESIKTWVQSGGVIAPMNDASLDWYTSESDRRMAKLVQDAST 410 N++P M+F E+ + ++ GG + P Y S+S R + ++ A+T Sbjct: 331 TATNNKPATTEFMKFLMHPEAHERFMGKGGFLTPHTGVDKGKYASDSFRGLHDILTGATT 390 Query: 411 LRFDGSDLMPGKVGAGTFWKGMTDYVSGTATLDKALEQIQSGWN 454 RFDGSDLMPG VGAG+FW GM DYV+G + D A + IQ+ W+ Sbjct: 391 FRFDGSDLMPGAVGAGSFWTGMVDYVNGKSAKDVA-DAIQASWD 433 >ref|NP_384801.1| ALPHA-GLUCOSIDES-BINDING PERIPLASMIC ABC TRANSPORTER PROTEIN [Sinorhizobium meliloti] sp|Q9Z3R5|AGLE_RHIME Alpha-glucosides-binding periplasmic protein aglE precursor emb|CAC45267.1| ALPHA-GLUCOSIDES-BINDING PERIPLASMIC ABC TRANSPORTER PROTEIN [Sinorhizobium meliloti] Length = 458 Score = 320 bits (821), Expect = 3e-86, Method: Compositional matrix adjust. Identities = 168/406 (41%), Positives = 248/406 (61%), Gaps = 7/406 (1%) Query: 55 EYKGTKVTMFGPFVDADQVKFESSIKEFEAKTGIDIQYEGSKEFEATINIRVDGGNAPDI 114 + KG +T+FGP+ D+ F+S F TG++++Y S+ +E I I G+ PD+ Sbjct: 53 DLKGQTLTIFGPWRGEDEALFKSVYAYFVEATGVELKYSSSENYEQQIVIDTQAGSPPDV 112 Query: 115 ADFPQPGLLASIAKTGKVVDLTGVLDQEKLKANY--NKSWLDMSTMDGKDG-KIMAGIWN 171 A PQPGL+A +A G + L G ++ L NY +SW+D+ST +GKDG + Sbjct: 113 AILPQPGLIADLAAKGLLTPL-GDETKQWLLDNYAAGQSWVDLSTYNGKDGTSALYAFPY 171 Query: 172 RSNVKSLVWYPKKQFDAAGYTVPETWDDMMALTEQIAKDGDPAWTIGIESGAATGWAATD 231 + +VKSLVWY + F+ AGY VP+T +++ ALTE+IA+DG+ W IG+ SG ATGW ATD Sbjct: 172 KIDVKSLVWYVPENFEDAGYEVPKTMEELKALTEKIAEDGEKPWCIGLGSGGATGWPATD 231 Query: 232 WIENIMLRTTTPENYDKWVKGELPFTSPEVKNAVEVMSKIWLNKDYVYGGTKSIVTTAFG 291 W+E++MLRT E YDKWVK E+PFT V A+E N +V GG ++ +T F Sbjct: 232 WVEDLMLRTQPAETYDKWVKNEIPFTDAAVTGALEEFGWFARNDAFVDGGAAAVASTDFR 291 Query: 292 DAPKPMFENPPKAWFNLMGNFITSFFPETAKVDQDYDWFYLPPIDP--QYGKPVLVAGDI 349 D+PK +F +PPK + + +FI SFFPE V +D D+FY+PP + + G PVL AG + Sbjct: 292 DSPKGLFSSPPKCYLHHQASFIPSFFPEGKVVGEDADFFYMPPYESKKELGNPVLGAGTL 351 Query: 350 YAMFNDRPEVRAVMEFFTTGESIKTWVQSGGVIAPMNDASLDWYTSESDRRMAKLVQDAS 409 + D P RA +EF T + + W+ + P ++D Y + ++ +++ +A+ Sbjct: 352 AMITKDTPAARAFIEFLKTPIAHEVWMAQTSFLTPYKSVNVDVYGNPPLKKQGEILLNAT 411 Query: 410 TLRFDGSDLMPGKVGAGTFWKGMTDYVSGTATLDKALEQIQSGWNN 455 T RFDGSDLMPGK+GAG FW GM D+V G ++ D A +Q W++ Sbjct: 412 TFRFDGSDLMPGKIGAGAFWTGMVDFVGGKSSADVA-AGVQKAWDS 456 >gb|AAD12050.1| AglE [Sinorhizobium meliloti] Length = 429 Score = 320 bits (821), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 168/406 (41%), Positives = 248/406 (61%), Gaps = 7/406 (1%) Query: 55 EYKGTKVTMFGPFVDADQVKFESSIKEFEAKTGIDIQYEGSKEFEATINIRVDGGNAPDI 114 + KG +T+FGP+ D+ F+S F TG++++Y S+ +E I I G+ PD+ Sbjct: 24 DLKGQTLTIFGPWRGEDEALFKSVYAYFVEATGVELKYSSSENYEQQIVIDTQAGSPPDV 83 Query: 115 ADFPQPGLLASIAKTGKVVDLTGVLDQEKLKANY--NKSWLDMSTMDGKDG-KIMAGIWN 171 A PQPGL+A +A G + L G ++ L NY +SW+D+ST +GKDG + Sbjct: 84 AILPQPGLIADLAAKGLLTPL-GDETKQWLLDNYAAGQSWVDLSTYNGKDGTSALYAFPY 142 Query: 172 RSNVKSLVWYPKKQFDAAGYTVPETWDDMMALTEQIAKDGDPAWTIGIESGAATGWAATD 231 + +VKSLVWY + F+ AGY VP+T +++ ALTE+IA+DG+ W IG+ SG ATGW ATD Sbjct: 143 KIDVKSLVWYVPENFEDAGYEVPKTMEELKALTEKIAEDGEKPWCIGLGSGGATGWPATD 202 Query: 232 WIENIMLRTTTPENYDKWVKGELPFTSPEVKNAVEVMSKIWLNKDYVYGGTKSIVTTAFG 291 W+E++MLRT E YDKWVK E+PFT V A+E N +V GG ++ +T F Sbjct: 203 WVEDLMLRTQPAETYDKWVKNEIPFTDAAVTGALEEFGWFARNDAFVDGGAAAVASTDFR 262 Query: 292 DAPKPMFENPPKAWFNLMGNFITSFFPETAKVDQDYDWFYLPPIDP--QYGKPVLVAGDI 349 D+PK +F +PPK + + +FI SFFPE V +D D+FY+PP + + G PVL AG + Sbjct: 263 DSPKGLFSSPPKCYLHHQASFIPSFFPEGKVVGEDADFFYMPPYESKKELGNPVLGAGTL 322 Query: 350 YAMFNDRPEVRAVMEFFTTGESIKTWVQSGGVIAPMNDASLDWYTSESDRRMAKLVQDAS 409 + D P RA +EF T + + W+ + P ++D Y + ++ +++ +A+ Sbjct: 323 AMITKDTPAARAFIEFLKTPIAHEVWMAQTSFLTPYKSVNVDVYGNPPLKKQGEILLNAT 382 Query: 410 TLRFDGSDLMPGKVGAGTFWKGMTDYVSGTATLDKALEQIQSGWNN 455 T RFDGSDLMPGK+GAG FW GM D+V G ++ D A +Q W++ Sbjct: 383 TFRFDGSDLMPGKIGAGAFWTGMVDFVGGKSSADVA-AGVQKAWDS 427 >ref|NP_105832.1| alpha-glucosides ABC transporter, substrate-binding protein [Mesorhizobium loti MAFF303099] dbj|BAB51618.1| alpha-glucosides ABC transporter, substrate-binding protein [Mesorhizobium loti] Length = 448 Score = 320 bits (819), Expect = 5e-86, Method: Compositional matrix adjust. Identities = 170/407 (41%), Positives = 242/407 (59%), Gaps = 7/407 (1%) Query: 53 KGEYKGTKVTMFGPFVDADQVKFESSIKEFEAKTGIDIQYEGSKEFEATINIRVDGGNAP 112 K + KG +T+FGP+ D+ + ++ F+ TG +I+Y S+ +E I I G+ P Sbjct: 41 KVDLKGETLTIFGPWRGEDEGLIRTVLEYFQEATGAEIKYSSSENYEQQIVIDTQAGSPP 100 Query: 113 DIADFPQPGLLASIAKTGKVVDLTGVLDQEKLKANYN--KSWLDMSTMDGKDGKI-MAGI 169 +IA PQPGL+ +A G + L G + +K NY +SW+D+ T KDGK Sbjct: 101 NIAVLPQPGLIQDLASKGLLTPL-GDDTAKWIKDNYGAGQSWVDLGTFKDKDGKPGFFAF 159 Query: 170 WNRSNVKSLVWYPKKQFDAAGYTVPETWDDMMALTEQIAKDGDPAWTIGIESGAATGWAA 229 +++VKSLVWY F AGYTVP+T +++ L ++I DG W IG+ SG ATGW A Sbjct: 160 PYKADVKSLVWYSPDNFKEAGYTVPKTQEELADLEKKIIADGGTPWCIGLGSGGATGWPA 219 Query: 230 TDWIENIMLRTTTPENYDKWVKGELPFTSPEVKNAVEVMSKIWLNKDYVYGGTKSIVTTA 289 TDW+E++MLRT PE YDKWVK E+PF P V NA+++ KI + V GG K++ T Sbjct: 220 TDWVEDLMLRTQPPEVYDKWVKNEIPFNDPAVVNAIDIFGKIATDDKMVDGGAKAVAATD 279 Query: 290 FGDAPKPMFENPPKAWFNLMGNFITSFFPETAKVDQDYDWFYLPPI--DPQYGKPVLVAG 347 F D+PK +F PPK + + +FI +FFP+ KV QD D+FY PP P+ G PVL AG Sbjct: 280 FRDSPKGLFSVPPKCYLHKQASFIPTFFPQGTKVGQDADFFYFPPYASKPELGTPVLGAG 339 Query: 348 DIYAMFNDRPEVRAVMEFFTTGESIKTWVQSGGVIAPMNDASLDWYTSESDRRMAKLVQD 407 + + D RA +EF + + W+ GG + P + + Y S++ R+ +++ + Sbjct: 340 TLAMITKDSKAARAFIEFLKMPLAHEIWMAQGGFVTPFKSVNKEAYASDALRKQGEILAN 399 Query: 408 ASTLRFDGSDLMPGKVGAGTFWKGMTDYVSGTATLDKALEQIQSGWN 454 AST RFDGSDLMPGK+GAG FW GM D V G + D A + IQ W+ Sbjct: 400 ASTFRFDGSDLMPGKIGAGAFWTGMIDLVGGKSAQDVATD-IQKSWD 445 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.134 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 676,134,109 Number of extensions: 28991099 Number of successful extensions: 72863 Number of sequences better than 1.0e-05: 144 Number of HSP's gapped: 72696 Number of HSP's successfully gapped: 144 Length of query: 455 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 326 Effective length of database: 406,989,807 Effective search space: 132678677082 Effective search space used: 132678677082 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)