BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0042.0008.p (367 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_623915.1| germination protein [Thermoanaerobacter tengcon... 85 2e-15 ref|ZP_00180438.1| hypothetical protein Mther161501 [Moorella th... 83 1e-14 ref|ZP_00097703.1| COG1115: Na+/alanine symporter [Desulfitobact... 82 3e-14 gb|AAB61395.1| germination protein 81 4e-14 ref|NP_388254.1| gerKB [Bacillus subtilis] >gi|1708044|sp|P49940... 73 1e-11 >ref|NP_623915.1| germination protein [Thermoanaerobacter tengcongensis] gb|AAM25519.1| germination protein [Thermoanaerobacter tengcongensis] Length = 369 Score = 85.1 bits (209), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 80/361 (22%), Positives = 156/361 (43%), Gaps = 7/361 (1%) Query: 2 NRKIGTTQAAMLIVNTILPTATVVLPAIISSYAEQDAPLAILLSTFFGLLIAYIVGSAVR 61 +KI T Q +V T++ TA V LP+ ++ A+QD+ +++++S I Sbjct: 13 EKKISTKQLVFFLVTTVISTADVFLPSFVALAAKQDSWISVIISFLVSSGIFVFYYKLAM 72 Query: 62 CTNGAPFLTWVGEKSSPVIAAILGLFMLQFYLDTSATILREFVNFLKDNVLINTPXXXXX 121 ++V + + ++ L F L + ++RE + + + + +TP Sbjct: 73 MFKEEILFSYVDKIVGSFLGKVISFLYLLFILHGISLVMRELIEIMVNAFMPHTPPIVFY 132 Query: 122 XXXXXXTIYMVRQGLEAIARVNSXXXXXXXXXXXXXXXGLANEMNVHQLLPMFDHSLADL 181 +Y V +G AI ++N ++++ LP+ ++ + Sbjct: 133 VVLMVSVMYTVSKGFLAIVKLNELLFPFGMLLLAFVIVLDLPKVDMRNFLPILENGIMAP 192 Query: 182 TLASITPTTWMSEVAVLLFMAPYLQAPRQARIIGWTGLIFVALLMMFSLVIALMVFGPDL 241 + S+ T +M E ++LF+ P++ +A G L +AL MM V+A+ +FG Sbjct: 193 IIGSVLITPFMLETVIILFIFPHISQKEKALKGGIISLGILALSMMLG-VLAIGIFG--- 248 Query: 242 VRLSAYPGFSAAGIV---HLGRFIEKLDILFISYWVLSIYVKFSIFLFATVECFKQTFKV 298 V+ +A F+A +V + +I++ D L ++ W++ IY+K IF + + +T K Sbjct: 249 VKTAANFQFTALEMVRNIRISDYIQRFDSLVMAMWLMGIYIKIVIFTYLFAKGLAETIKS 308 Query: 299 GSSRPFIGTLGLLIVLECLYTWESTEKLNLYNKEGRFLVFFIFNVLVPLGAVGLDRFTKA 358 R + L L++ ES + L Y + F F V PL + + K Sbjct: 309 TDYRFILLPLATLLIPLAENVSESLDGLYTYFQRCFPFEGFWFEVFFPLLLYTIAKIRKI 368 Query: 359 K 359 K Sbjct: 369 K 369 >ref|ZP_00180438.1| hypothetical protein Mther161501 [Moorella thermoacetica ATCC 39073] Length = 281 Score = 82.8 bits (203), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 54/229 (23%), Positives = 104/229 (45%), Gaps = 7/229 (3%) Query: 86 LFMLQFYLDTSATILREFVNFLKDNVLINTPXXXXXXXXXXXTIYMVRQGLEAIARVNSX 145 +F+L F+L +A ++REF FL V+ TP IY R GLE IAR Sbjct: 5 VFVLGFFL-LNAVVIREFSEFLTTAVMPETPCLFFAFSIVAVAIYAARNGLEVIARCTDF 63 Query: 146 XXXXXXXXXXXXXXGLANEMNVHQLLPMFDHSLADLTLASITPTTWMSEVAVLLFMAPYL 205 EM++ + P ++ + + ++ ++ +V +L ++ Sbjct: 64 IMPLLVAFIFVILLFSTPEMSLRNIFPFLENGIRPVLGGTLITWPFLGQVIILATYGAFV 123 Query: 206 QAPRQARIIGWT---GLIFVALLMMFSLVIALMVFGPDLVRLSAYPGFSAAGIVHLGRFI 262 PR +GW+ GL+ +A + + ++VFG + G++ A +V LG+F+ Sbjct: 124 NPPRS---LGWSLISGLVGIAFFLGLVTMGVILVFGACEASSLTFAGYNLARVVSLGQFL 180 Query: 263 EKLDILFISYWVLSIYVKFSIFLFATVECFKQTFKVGSSRPFIGTLGLL 311 E++++LF++ WV +++K ++ + + RP + LG L Sbjct: 181 ERIEVLFLAIWVAGVFIKITLNFYVVALGLATVTGLKEYRPLVAPLGAL 229 >ref|ZP_00097703.1| COG1115: Na+/alanine symporter [Desulfitobacterium hafniense] Length = 387 Score = 81.6 bits (200), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 64/324 (19%), Positives = 142/324 (43%) Query: 4 KIGTTQAAMLIVNTILPTATVVLPAIISSYAEQDAPLAILLSTFFGLLIAYIVGSAVRCT 63 +I +Q A+L+ TI+ TA +V+P+I + A Q A + L+ G L ++ R Sbjct: 28 RISASQFAVLLFLTIMATAVLVVPSITAKTAGQSAWMVPALAAGLGCLTLWLTTKLNRRF 87 Query: 64 NGAPFLTWVGEKSSPVIAAILGLFMLQFYLDTSATILREFVNFLKDNVLINTPXXXXXXX 123 + + + + ++G+ + F+L S I+++ L +L NTP Sbjct: 88 PEKNLIQYSRTIAGKYLGKMIGIGYMIFFLVFSFLIVQQVAEVLYFFLLKNTPPWFLALC 147 Query: 124 XXXXTIYMVRQGLEAIARVNSXXXXXXXXXXXXXXXGLANEMNVHQLLPMFDHSLADLTL 183 Y V QG+E IAR +M + +L+P+ + + + Sbjct: 148 FILVCSYGVFQGIEVIARTAQFVLPLFVLSFLSIVFFAFQDMQLKELMPLLEGGMYPIIR 207 Query: 184 ASITPTTWMSEVAVLLFMAPYLQAPRQARIIGWTGLIFVALLMMFSLVIALMVFGPDLVR 243 AS+ P +W E +L F+ P++ ++ G+ ++ A+ ++++ +++ GP+L Sbjct: 208 ASVVPGSWFGECIILAFLFPHIHKQKEIFRQGFWAILAAAVFFTGNILLTVLILGPELTA 267 Query: 244 LSAYPGFSAAGIVHLGRFIEKLDILFISYWVLSIYVKFSIFLFATVECFKQTFKVGSSRP 303 P + + +G +++ + + W+ ++ VKF++ + + F S + Sbjct: 268 NYDLPFWLVIRFIEVGSTFLRIETVIVFLWISAVVVKFTLIYYLGCVTTAEVFGWKSYQR 327 Query: 304 FIGTLGLLIVLECLYTWESTEKLN 327 + GL++ + ++ +LN Sbjct: 328 IVIPFGLVLAAASNFLLNNSMELN 351 >gb|AAB61395.1| germination protein Length = 329 Score = 80.9 bits (198), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 83/319 (26%), Positives = 148/319 (46%), Gaps = 17/319 (5%) Query: 4 KIGTTQAAMLIVNTILPTATVVLPAIISSYAEQDAPLAIL---LSTFFGLLIAYIVGSAV 60 KI TQ +L+V L +A +V P ++ A+QDA LAIL L +FF LI + + S Sbjct: 5 KISATQLFILMVLFELGSALLV-P--LAMGAKQDAWLAILIGMLCSFFFFLIYHKLYSYY 61 Query: 61 RCTNGAPFLTWVGEKSSPVIAAILGLFMLQFYLDTSATILREFVNFLKDNVLINTPXXXX 120 ++ + K V+ +L + ++L ++ +LR+F L +TP Sbjct: 62 PDLLPTEYMQKILGK---VLGTLLAFIYIVYFLYDASRVLRDFGEMLLTFAYPDTPLFIA 118 Query: 121 XXXXXXXTIYMVRQGLEAIARVNSXXXXXXXXXXXXXXXGLANE--MNVHQLLPMFDHSL 178 IY+ R+G+EAIAR + + ++ L P+ + Sbjct: 119 NALLMLVIIYITRKGIEAIARSGELLFIFMYFLAIAGFILIISSGLIDFKNLQPVLKEGI 178 Query: 179 ADLTLASITPTTWM--SEVAVLLFMAPYLQAPRQARI--IGWTGLIFVALLMMFSLVIAL 234 + ++T T + +E V + PYL P++A + + TGL + L++ +++I + Sbjct: 179 LPVAKVALTETLYFPFTEAIVFTMLLPYLSNPKKANVTMLCATGLSGINLII--TMIINI 236 Query: 235 MVFGPDLVRLSAYPGFSAAGIVHLGRFIEKLDILFISYWVLSIYVKFSIFLFATVECFKQ 294 V G DL S +P S + + F+E+LD+ F+ V+ IY K SI +A++ Sbjct: 237 SVLGVDLTTRSQFPLLSTVQTIQVADFLERLDVFFMFAMVIGIYFKVSILFYASIISTAT 296 Query: 295 TFKVGSSRPFIGTLGLLIV 313 FK+ S LG++++ Sbjct: 297 LFKIKSPSRLAYPLGIIVL 315 >ref|NP_388254.1| gerKB [Bacillus subtilis] sp|P49940|GRKB_BACSU Spore germination protein KB pir||I39860 germination response protein gerKB - Bacillus subtilis dbj|BAA11256.1| GerKB [Bacillus subtilis] dbj|BAA09004.1| spore germination protein GerKB [Bacillus subtilis] emb|CAB12180.1| gerKB [Bacillus subtilis subsp. subtilis str. 168] Length = 373 Score = 72.8 bits (177), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 67/329 (20%), Positives = 143/329 (43%), Gaps = 8/329 (2%) Query: 4 KIGTTQAAMLIVNTILPTATVVLPAIISSYAEQDAPLAILLSTFFGLLIAYIVGSAVRCT 63 +I Q ++I+ L ++ ++ P S A +DA +A+LL GL + Y+ +C Sbjct: 5 RISIRQLFVMIIIFELGSSLLITPG---SMAGRDAWIAVLLGCAIGLFLFYLYQGIYQCY 61 Query: 64 NGAPFLTWVGEKSSPVIAAILGLFMLQFYLDTSATILREFVNFLKDNVLINTPXXXXXXX 123 + ++ + ++ + + ++ +A +LR+F L +TP Sbjct: 62 PNSSPKEYMDDMLGTKLSWLFSFLYILYFAYIAARVLRDFGEMLLTFAYHDTPIIIVNAL 121 Query: 124 XXXXTIYMVRQGLEAIARVNSXX--XXXXXXXXXXXXXGLANEMNVHQLLPMFDHSLADL 181 +IY VR+G+E +AR ++ ++ H L P+ + ++ + Sbjct: 122 LMVVSIYAVRKGIEVLARAAELLFGAMYLLGAIGLVLIIVSGTIDPHNLKPVLANGISPV 181 Query: 182 TLASITPTTWM--SEVAVLLFMAPYLQAPRQARIIGWTGLIFVALLMMFSLVIALMVFGP 239 + T T ++ EV + + + P L + + +G + L++ ++ I + V Sbjct: 182 LHSVFTQTMYVPFGEVVLFVMIFPNLNDRKDVKKMGMIAMAISGLIVALTVAINISVLDV 241 Query: 240 DLVRLSAYPGFSAAGIVHLGRFIEKLDILFISYWVLSIYVKFSIFLFATVECFKQTFKVG 299 DL S +P S + + F+++LD+ F+ ++ + K S++L+ATV FK Sbjct: 242 DLTLRSQFPLLSTIQTIKVEEFLDRLDVFFMLALIIGGFFKVSLYLYATVVGTSTLFKEK 301 Query: 300 SSRPFIGTLGL-LIVLECLYTWESTEKLN 327 + +GL +++L +E LN Sbjct: 302 NPSQLAYPMGLGILILSITIATNFSEHLN 330 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.329 0.142 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 454,113,988 Number of extensions: 16933179 Number of successful extensions: 61940 Number of sequences better than 1.0e-05: 33 Number of HSP's gapped: 61914 Number of HSP's successfully gapped: 33 Length of query: 367 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 240 Effective length of database: 412,420,005 Effective search space: 98980801200 Effective search space used: 98980801200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 126 (53.1 bits)