BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0042.0007.p (410 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00181180.1| hypothetical protein Mther075801 [Moorella th... 117 6e-25 ref|NP_149183.1| Spore germination protein, GRKC [Clostridium ac... 115 2e-24 ref|ZP_00097484.1| hypothetical protein [Desulfitobacterium hafn... 113 8e-24 gb|AAB61394.1| germination protein 110 8e-23 ref|NP_623913.1| germination protein [Thermoanaerobacter tengcon... 108 2e-22 >ref|ZP_00181180.1| hypothetical protein Mther075801 [Moorella thermoacetica ATCC 39073] Length = 416 Score = 117 bits (292), Expect = 6e-25, Method: Compositional matrix adjust. Identities = 93/399 (23%), Positives = 176/399 (44%), Gaps = 27/399 (6%) Query: 23 GCWDDRELSELGITSGSAYDWEDNQWKATYQVINPXXXXXXXXXXXXXXXXXPPFVTFTV 82 GCWD RE++EL S +A+D E T + I P + + Sbjct: 25 GCWDRREINELAFLSCAAFDLEGGNRVLTSEFIRPSAAGGGERGGGETLPQRQALIV-SN 83 Query: 83 KGRTIMEAVEKTNLTSTREMFFSHSRITVMGESVARHGVNQLIDLFLRKQDARETVFVFI 142 + +T + + L R + +H+ ++GE +AR+G+ +++D R + R T + + Sbjct: 84 RNKTFLAIGREKALGLPRRAYLAHTAAVLVGEEMARYGIKEVLDFVDRNPEIRRTTLILL 143 Query: 143 AKGEAGDILDQLMQ-LTKNQG---AGIQLMIEQESRLLSYYPGTRMFELAMALSSESGSA 198 +G A ++L + L K G G+ ++ Y P + ++ LS ++G+ Sbjct: 144 TRGPAREVLVRAQSGLEKTLGREITGLHKWVQVSG--YGYIP--NINDIFFDLSGDAGTT 199 Query: 199 VLPEILLTG---PEIMDETQET------GRTDLQTRLALGRL---GVLKGEKLVGWLSQK 246 VLP + L+ P I+ T GR L RL G+ +KLV WL Q+ Sbjct: 200 VLPVLELSPQPFPPILGPATATGGGIPAGRAGEPETLMTARLNGAGLFYHDKLVAWLDQE 259 Query: 247 QAFGLSFMTDKIKTATIAFPSKPESGGKPDASFVLQKSRTTVQPKWEKDHYVIDMNIKGS 306 Q G +++ +K+K+A +A P + S S + SR + + IK Sbjct: 260 QTRGWAWVRNKVKSAMLALPRQENS----LVSVNIISSRAEAAIDMQGGRPQGKIKIKVE 315 Query: 307 GVLTELGSVMDLNERASITEMEQSLEQQVLELVDNSWKEVRKLGADVTGFAIKIHRSDRK 366 G L E D + ++ +E + Q+ + ++ + + G DV GF +HR + Sbjct: 316 GDLLEEQCYQDFTKEEAVKSLESRMAAQITSEISSALNQAKMAGTDVFGFGGALHRRYPE 375 Query: 367 RFKQIRKDKSWDRVFQEIEIRPHVSIEIERTGLSNKSFQ 405 ++Q+ + W+ F+++ + V ++ RTG++ + +Q Sbjct: 376 VWRQL--EGRWNEEFKKLPVTISVEAKLRRTGMTGRPWQ 412 >ref|NP_149183.1| Spore germination protein, GRKC [Clostridium acetobutylicum] gb|AAK76765.1| Spore germination protein, GRKC [Clostridium acetobutylicum] Length = 394 Score = 115 bits (288), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 91/379 (24%), Positives = 174/379 (45%), Gaps = 19/379 (5%) Query: 23 GCWDDRELSELGITSGSAYDWEDN--QWKATYQVINPXXXXXXXXXXXXXXXXXPPF-VT 79 C D REL++L I D +N + T Q+ + Sbjct: 23 SCIDSRELNKLAIVLAVGIDKLENCDDTEMTIQLAKLSSNKSGSISSGGSAPSANNVTLN 82 Query: 80 FTVKGRTIMEAVEKTNLTSTREMFFSHSRITVMGESVARHGVNQLIDLFLRKQDARETVF 139 T G + + V+ N R++FFSH+++ V+GES A+ G+ + +D F+R ++ R V+ Sbjct: 83 LTETGNGLGKIVKTFNRKLDRQLFFSHNQVIVLGESAAKSGIAKYLDFFMRYRETRLLVW 142 Query: 140 VFIAKGEAGDILDQLMQLTKNQGAGIQLMIEQESRLLSYYPGTRMFELAMALSSESGSAV 199 + I K + DIL + QG + +I E + +S P + + + S++ + + Sbjct: 143 LLITKSKVKDILSVPPNMEDTQGRNLAELI-SEQKEVSEIPAINLKDFTERIMSKTSAPI 201 Query: 200 LPEILLTGPEIMDETQETGRTDLQTRLALGRLGVLKGEKLVGWLSQKQAFGLSFMTDKIK 259 +P + ++ T + L L V K +K++G L +Q GL + +K+K Sbjct: 202 IPIVEIS----------TNNSAGTKALRLTSTAVFKKDKMIGTLDSQQTRGLLWALNKVK 251 Query: 260 TATIAFPSKPESGGKPDASFVLQKSRTTVQPKWEKDHYVIDMNIKGSGVLTELGSVMDLN 319 T + + P G D + K + + K + + ++ +NIK G L E S +L Sbjct: 252 TGVVPV-NLPGYKGTIDVETIHSKGK--IAAKLKDNKLIMKINIKQEGNLLEQNSSENLA 308 Query: 320 ERASITEMEQSLEQQVLELVDNSWKEVRKLGADVTGFAIKIHRSDRKRFKQIRKDKSWDR 379 + +E++ E+ + + ++ + + R L AD+ GF I+R K FK+I + W++ Sbjct: 309 TPDNFKVIEKAEEETIKKQIEAAILKSRDLNADIFGFGDAIYRRHPKYFKKI--EPQWNK 366 Query: 380 VFQEIEIRPHVSIEIERTG 398 F+ + I +V+ ++ R G Sbjct: 367 TFKNLNIEVNVNCKLRRNG 385 >ref|ZP_00097484.1| hypothetical protein [Desulfitobacterium hafniense] Length = 389 Score = 113 bits (282), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 92/379 (24%), Positives = 169/379 (44%), Gaps = 26/379 (6%) Query: 23 GCWDDRELSELGITSGSAYD--WEDNQWKATYQVINPXXXXXXXXXXXXXXXXXPPFVTF 80 GCW+ REL+ L I G D E NQ + T Q+ NP F Sbjct: 23 GCWNQRELNSLAIVMGLGIDKAQEPNQIQVTAQIANPKALKSSSLGGPDSQA----FWNI 78 Query: 81 TVKGRTIMEAVEKTNLTSTREMFFSHSRITVMGESVARHGVNQLIDLFLRKQDARETVFV 140 G T+ + S R+++F H ++ ++G A G+ + +D F R + R +++ Sbjct: 79 QNTGDTLFSILRDFTHESNRKLYFPHIQVLILGRDYAEEGIQKELDFFARDHETRLNIWL 138 Query: 141 FIAKGEAGDILDQLMQLTKNQGAGI-QLMIEQESRLLSYYPGTRMFELAMALSSESGSAV 199 +++G A D+L+ + + I +L+ +Q+ + F + + + +A Sbjct: 139 VVSEGNARDVLNIKPVIDRIPAINISKLLDDQDPNSQTSTSQLNDFLSDLISPTTAPTAP 198 Query: 200 LPEILLTGPEIMDETQETGRTDLQTRLALGRLGVLKGEKLVGWLSQKQAFGLSFMTDKIK 259 L EI+ G G LQ + V KG+KLVG L++ + GL + D++K Sbjct: 199 LVEIIRKG----------GNESLQVK----GTAVFKGDKLVGELNKYETRGLLWARDEVK 244 Query: 260 TATIAFPSKPESGGKPDASFVLQKSRTTVQPKWEKDHYVIDMNIKGSGVLTELGSVMDLN 319 + I PE G K S + S+T V + + ++ + I+ G L +L Sbjct: 245 SGIIDVEC-PECGSK--VSLEIIDSKTKVSAEIVDNQVIMKVYIQEKGALAAQYCAENLR 301 Query: 320 ERASITEMEQSLEQQVLELVDNSWKEVRKLGADVTGFAIKIHRSDRKRFKQIRKDKSWDR 379 + + +E++ + + V ++K+ ++L AD+ GF + + K +K ++ + WD Sbjct: 302 TKEKVAAIERNKAAAIQDEVLAAFKKAQELNADIFGFGEVVRKKHLKEWKNMQ--EHWDE 359 Query: 380 VFQEIEIRPHVSIEIERTG 398 +F EI + V IER+G Sbjct: 360 IFPEIIVEVTVDASIERSG 378 >gb|AAB61394.1| germination protein Length = 408 Score = 110 bits (274), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 98/392 (25%), Positives = 181/392 (46%), Gaps = 11/392 (2%) Query: 24 CWDDRELSELGITSGSAYD-WEDNQWKATYQVINPXXXXXXXXXXXXXXXXXPPFVTFTV 82 CW +EL++L S D +D +++ T+Q+INP PP ++ Sbjct: 21 CWSKKELTDLSFVSAMGIDKTKDGRYELTFQIINPGNVAGLQGGGGTQS---PPVTIYSA 77 Query: 83 KGRTIMEAVEKTNLTSTREMFFSHSRITVMGESVARH-GVNQLIDLFLRKQDARETVFVF 141 G ++EA + + +R ++++H+ + V+GE +AR G+N LID R + R T + Sbjct: 78 SGDNLVEASRRASARISRRLYYAHTNLVVIGEKLAREEGINILIDSIDRDPEFRITTSLV 137 Query: 142 IA-KGEAGDILDQLMQLTKNQGAGIQLMIEQESRLLSYYPGTRMFELAMALSSESGSAVL 200 IA + A D++ L + K I +E R E+ +L S G A++ Sbjct: 138 IANQSSAADLVKALTPVDKIPANKIMKTLEFTQRKWGENLKVSFQEVMRSLESPGGEALV 197 Query: 201 PEILLTG-PEIMDETQETGRTDLQTRLALGRLGVLKGEKLVGWLSQKQAFGLSFMTDKIK 259 L G P+ ++ ++ + L + V K KL+ WL A G ++ DKI+ Sbjct: 198 SGFRLVGNPQQAEKLDNLQQSAPEATLRASGIAVFKNGKLIDWLYNSPARGSVWILDKIQ 257 Query: 260 TATIAFPSKPESGGKPDASFVLQKSRTTVQPKWEKDHYVIDMNIKGSGVLTELGSVMDLN 319 I K + K ++ + +T V K K ++ + G L E+ +D Sbjct: 258 GTDINVDWKEK---KEALAYQTVRQKTDVSVKMIKGKPYASVHTRVEGDLGEVEVPVDTR 314 Query: 320 ERASITEMEQSLEQQVLELVDNSWKEVRKLGADVTGFAIKIHRSDRKRFKQIRKDKSWDR 379 + A I +ME++ +++ + + + K+ + D+ GF + R+ ++++++ + D Sbjct: 315 QPAVIKKMEKAAGKEIKKELQTAIKQAQTDKVDIFGFGEALERTRPNQWEKLKSEWDDDV 374 Query: 380 VFQEIEIRPHVSIEIERTGLSNKSF-QSVHEK 410 F ++++ V I RTGL NKSF SV EK Sbjct: 375 YFPKLDVDVTVEAYIRRTGLRNKSFLSSVKEK 406 >ref|NP_623913.1| germination protein [Thermoanaerobacter tengcongensis] gb|AAM25517.1| germination protein [Thermoanaerobacter tengcongensis] Length = 399 Score = 108 bits (270), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 93/396 (23%), Positives = 170/396 (42%), Gaps = 33/396 (8%) Query: 23 GCWDDRELSELGITSGSAYD-WEDNQWKATYQVINPXXXXXXXXXXXXXXXXX---PPFV 78 GCWD RE+++L G + ED +++ P P+ Sbjct: 22 GCWDKREINQLAFVQGLGIEKGEDGMIHLVVEILKPGLLASGGGPGGGTGGGSAVGKPYA 81 Query: 79 TFTVKGRTIMEAVEKTNLTSTREMFFSHSRITVMGESVARHGVNQLIDLFLRKQDARETV 138 F G +A N R +F ++ I + E AR G+ Q +D R + R T Sbjct: 82 VFQANGVDFAKAFANLNDELPRSLFMQYNEIIFLDEKFARSGIYQTLDFMTRNPEFRRTA 141 Query: 139 FVFIAKGEAGDILDQLMQLTKNQGAGIQLMIEQESRLLSYYPGTRMFELAMALSSESGSA 198 ++ + G G+ +L S L YP + + ++ S S Sbjct: 142 YILVVTG----------------GSIHELFNIPSSSTLERYPYKEVLGIISNQANTSSSY 185 Query: 199 V--LPEILLTGPEIMDETQETGRTDLQTR--LALG-RL---GVLKGEKLVGWLSQKQAFG 250 V L E + T EI + TG+ ++ + A+G RL V +KL+G+L ++ Sbjct: 186 VCDLNEFIQT-LEIPKKAPITGKLEIVKKDGKAVGVRLVGSAVFDNDKLIGFLEEQDTKA 244 Query: 251 LSFMTDKIKTATIAFPSKPESGGKPDASFVLQKSRTTVQPKWEKDHYVIDMNIKGSGVLT 310 ++ + +K+K +T+ K G K S V+ K++T ++P E D ++ + L Sbjct: 245 VTVLMNKLKRSTLTL-DKGLKGEKAHISLVITKAKTEIKPVVEGDKISFNIKVNVEAYLN 303 Query: 311 ELGSVMDLNERASITEMEQSLEQQVLELVDNSWKEV-RKLGADVTGFAIKIHRSDRKRFK 369 E + D+ E + ++E + ++ ++ + + +K ADV GF +HRS K +K Sbjct: 304 EQETKYDITEPEKLQQLEVIVSSKIKSAIERALNILQKKYNADVVGFVEILHRSKPKVWK 363 Query: 370 QIRKDKSWDRVFQEIEIRPHVSIEIERTGLSNKSFQ 405 ++ +D W+ ++ +I+ V I R+GLS+K Sbjct: 364 KVVED--WENIYPDIKFNVTVKTHIRRSGLSSKPIH 397 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.132 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 509,198,597 Number of extensions: 18611343 Number of successful extensions: 50976 Number of sequences better than 1.0e-05: 34 Number of HSP's gapped: 50936 Number of HSP's successfully gapped: 35 Length of query: 410 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 282 Effective length of database: 409,704,906 Effective search space: 115536783492 Effective search space used: 115536783492 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)