BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0011.p (462 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_952746.1| threonine synthase [Geobacter sulfurreducens PC... 480 e-134 gb|EAI36596.1| unknown [environmental sequence] 453 e-126 ref|ZP_00044164.1| COG0498: Threonine synthase [Magnetococcus sp... 443 e-123 ref|ZP_00079483.1| COG0498: Threonine synthase [Geobacter metall... 422 e-117 gb|EAK39578.1| unknown [environmental sequence] 404 e-111 >ref|NP_952746.1| threonine synthase [Geobacter sulfurreducens PCA] gb|AAR35073.1| threonine synthase [Geobacter sulfurreducens PCA] Length = 461 Score = 480 bits (1235), Expect = e-134, Method: Compositional matrix adjust. Identities = 234/463 (50%), Positives = 311/463 (67%), Gaps = 7/463 (1%) Query: 1 MEYISTRGNVEAKGFIDTVLMGLADDGGLMVPFRIPVISPEKLAEWRSLSFQELFLEIFS 60 M YISTRG ++ F D V+MGLA DGGL++P IP I + LA W++L FQEL I S Sbjct: 1 MRYISTRGTIQPIRFKDAVMMGLATDGGLLLPETIPAIDRDTLAAWKTLPFQELAFRIIS 60 Query: 61 YYTNDEIPYEDLKEMVYTSYANFRAPEVTPLHQVNDSLYVLELFHGPTFAFKDVALQFMG 120 Y D+IP +DL+ ++ SYA F P+VTP+ + L++LELFHGPT AFKDVALQF+G Sbjct: 61 RYA-DDIPADDLRSLIERSYATFDHPDVTPVVE-RGGLHILELFHGPTLAFKDVALQFLG 118 Query: 121 ELYSYISRKRGEVIHILGATSGDTGAAAIQGVRGKEGIKICILHPHGKVSKVQELQMTTV 180 L+ Y+ ++R E ++I+GATSGDTG+AAI GVRGKE I I ILHPHGK S VQ LQMTTV Sbjct: 119 NLFEYLLQERNERMNIVGATSGDTGSAAIYGVRGKENINIFILHPHGKTSPVQALQMTTV 178 Query: 181 DDSNVLNLSVEGNFDDCQKVIKELFADLDFKGKYHLRAINSINFVRILAQTVYYFYAYLQ 240 D NV N++V G FDDCQ ++K LF DL FK +Y L A+NSIN+ R+LAQ VYYF +Y + Sbjct: 179 LDPNVHNIAVRGTFDDCQNIVKSLFNDLPFKERYALGAVNSINWARVLAQVVYYFLSYFR 238 Query: 241 IEDSDKKKINISVPSGNFGNIFSGYLAKKMGLPIHKLIIATNENNILERFVNTGEYKPGS 300 + + ++ SVP+GNFG+IF+GY+AK+MGLPI +L++ATNENNIL RF+N G+Y + Sbjct: 239 VAKTIGDEVVFSVPTGNFGDIFAGYVAKRMGLPIARLLLATNENNILARFINDGDYSLSA 298 Query: 301 FTSTYSPSMDIQVASNFERYLYYLVGEDAEKLSAHMSKLQTEGKITIAGEALQQVQADFA 360 T SPSMDIQ+ASNFERY+YYL GED ++ + L G+I + ++ V+ +F Sbjct: 299 VVPTVSPSMDIQLASNFERYVYYLFGEDPARVREAFATLPARGRIVFSDAEMEHVRTEFL 358 Query: 361 ALGVKNQQCLDTIGKYNKEYDYLLDPHTACGIAAYEKFNGAGETAITFATAHPAKF-DEA 419 A V Q+ +DTI +N+E YLLDPHTA G+ A + G I ATAHPAKF D Sbjct: 359 ACSVNQQETVDTIASFNRETGYLLDPHTAVGVRAARQLVTDGTPVICLATAHPAKFADAV 418 Query: 420 IALVDIRQEFPEQIAALFSLPQHQTVVKNDKDVIVRELQAFYE 462 + V P + + LP V+ D + +++ F E Sbjct: 419 VRAVGFEPPRPPSLMGIEDLPSRCEVL----DARIEQIRTFIE 457 >gb|EAI36596.1| unknown [environmental sequence] Length = 461 Score = 453 bits (1165), Expect = e-126, Method: Compositional matrix adjust. Identities = 223/454 (49%), Positives = 309/454 (68%), Gaps = 4/454 (0%) Query: 1 MEYISTRGNVEAKGFIDTVLMGLADDGGLMVPFRIPVISPEKLAEWRSLSFQELFLEIFS 60 M Y STRG V+ F D V+MGLADDGGL+VP +P + L +WR LSF EL LEI Sbjct: 1 MRYSSTRGQVKNLMFEDAVMMGLADDGGLLVPNELPFVE-SNLDKWRLLSFTELSLEIML 59 Query: 61 YYTNDEIPYEDLKEMVYTSYANFRAPEVTPLHQVNDSLYVLELFHGPTFAFKDVALQFMG 120 +T+ IP E+ MV SYA+FR PE+TP+ V L++LELFHGPTFAFKDVALQF+G Sbjct: 60 LFTSGRIPREEFMRMVKQSYASFRHPEITPVKSVG-KLHILELFHGPTFAFKDVALQFLG 118 Query: 121 ELYSYISRKRGEVIHILGATSGDTGAAAIQGVRGKEGIKICILHPHGKVSKVQELQMTTV 180 L+++ ++R + ILGATSGDTG+AAI G+RGK+GI + +L+P G+VS +QE QMTTV Sbjct: 119 NLFAFFLKQRNHPLRILGATSGDTGSAAIHGLRGKQGIDVFMLYPKGRVSAIQERQMTTV 178 Query: 181 DDSNVLNLSVEGNFDDCQKVIKELFADLDFKGKYHLRAINSINFVRILAQTVYYFYAYLQ 240 DSN+ N++VEG+FDD Q ++K +F D FK ++L ++NSIN+ RILAQ VYYFYA+ + Sbjct: 179 LDSNIHNIAVEGSFDDAQGIVKAIFNDQKFKQTFNLGSVNSINWARILAQIVYYFYAWFR 238 Query: 241 IEDSDKKKINISVPSGNFGNIFSGYLAKKMGLPIHKLIIATNENNILERFVNTGEYKPGS 300 + + +++++ VP+GNFGN+ +GY AK MGLPI KLI+ TNEN+IL RF + GEY Sbjct: 239 VTNDIRERVSFVVPTGNFGNVMAGYYAKLMGLPIDKLIVGTNENDILHRFFSRGEYHYTE 298 Query: 301 FTSTYSPSMDIQVASNFERYLYYLVGEDAEKLSAHMSKLQTEGKITIAGEALQQVQADFA 360 T SPSMDIQ++SNFERYLY L G +++L M + + G++T+ ++ Q DFA Sbjct: 299 VKETLSPSMDIQISSNFERYLYDLTGNSSKQLQLWMDEFEKSGELTVESNLQKKAQQDFA 358 Query: 361 ALGVKNQQCLDTIGKYNKEYDYLLDPHTACGIAAYEKFNGAGETAITFATAHPAKFDEAI 420 + V +++ L I K+++E+ YLLDPH+A G+ A EK I A AHPAKF AI Sbjct: 359 SARVDDEEILSIIRKFHQEHKYLLDPHSAVGVCAAEKIE-ILTPVICLACAHPAKFSMAI 417 Query: 421 -ALVDIRQEFPEQIAALFSLPQHQTVVKNDKDVI 453 + + PE++A+L +L V D + + Sbjct: 418 RKALGTEPKLPEELASLHNLKTRGKTVPADPEAV 451 >ref|ZP_00044164.1| COG0498: Threonine synthase [Magnetococcus sp. MC-1] Length = 459 Score = 443 bits (1140), Expect = e-123, Method: Compositional matrix adjust. Identities = 229/453 (50%), Positives = 304/453 (67%), Gaps = 17/453 (3%) Query: 1 MEYISTRGNVEAKGFIDTVLMGLADDGGLMVPFRIPVISPEKLAEWRSLSFQELFLEIFS 60 M YISTRG V F + V+MGLA DGGL+VP R+P + L W LS+QEL LE+ Sbjct: 1 MRYISTRGGVAPLSFSEAVMMGLATDGGLLVPERLPQVDGATLRRWAKLSYQELALEVMR 60 Query: 61 YYTNDEIPYEDLKEMVYTSYANFRAPEVTPLHQVNDSLYVLELFHGPTFAFKDVALQFMG 120 + ++IP DL+ +V +YA F E+TPL +V D + +LELFHGPT AFKDVALQF+G Sbjct: 61 PFVEEDIPEADLRPLVINAYAKFEHDEITPLVKVGD-VQILELFHGPTLAFKDVALQFLG 119 Query: 121 ELYSYISRKRGEVIHILGATSGDTGAAAIQGVRGKEGIKICILHPHGKVSKVQELQMTTV 180 L+ Y+ K+G ++I+GATSGDTG+AAI GVRGK GI I ILHPH +VS VQE QMTTV Sbjct: 120 NLFEYLLEKQGGRLNIIGATSGDTGSAAIHGVRGKSGIDIFILHPHQRVSPVQERQMTTV 179 Query: 181 DDSNVLNLSVEGNFDDCQKVIKELFADLDFKGKYHLRAINSINFVRILAQTVYYFYAYLQ 240 D NV N+++EGNFDD Q ++K LF DL FK KYHL A+NSIN+ RILAQ VYYF + + Sbjct: 180 LDDNVHNIAIEGNFDDGQAIVKALFNDLAFKEKYHLGAVNSINWARILAQIVYYFRGWAR 239 Query: 241 IEDSD-KKKINISVPSGNFGNIFSGYLAKKMGLPIHKLIIATNENNILERFVNTGEYKPG 299 + ++K++ SVP+GNFG+I++GYLA++MGLP+ +LI+ATN N+IL RFV G Y G Sbjct: 240 ATGGNPEQKVSFSVPTGNFGDIYAGYLARQMGLPVDRLILATNRNDILSRFVLHGSYSKG 299 Query: 300 SFTSTYSPSMDIQVASNFERYLYYLVGEDAEKLSAHMSKLQTEGKITIAGEALQQVQADF 359 T SPSMDIQ++SNFERYL+ L+ D + + M++L +G +++ E L QV+ F Sbjct: 300 VVEPTISPSMDIQISSNFERYLFDLLERDGDGVKGCMAELAEQGGFSVSAEKLAQVRTIF 359 Query: 360 AALGVKNQQCLDTIGKYNKEYDYLLDPHTACGIAAYEKFNGAGETAITFATAHPAKFDEA 419 A+ V Q L+TI + ++E Y+LDPHTA G+AA G I ATAHPAKF +A Sbjct: 360 DAVAVSEAQTLETITRVHRESGYVLDPHTAVGVAAAAGRQG----VICLATAHPAKFADA 415 Query: 420 IALVDIRQ------EFPEQIAALFSLPQHQTVV 446 +RQ P + L +LP V+ Sbjct: 416 -----VRQATGEAVALPPSMQDLMTLPTRCEVL 443 >ref|ZP_00079483.1| COG0498: Threonine synthase [Geobacter metallireducens] Length = 404 Score = 422 bits (1085), Expect = e-117, Method: Compositional matrix adjust. Identities = 205/399 (51%), Positives = 278/399 (69%), Gaps = 6/399 (1%) Query: 65 DEIPYEDLKEMVYTSYANFRAPEVTPLHQVNDSLYVLELFHGPTFAFKDVALQFMGELYS 124 D+IP +DLK+++ SYA F PE+TP+ + D +Y+LELFHGPT AFKDVALQ +G L+ Sbjct: 7 DDIPAQDLKDLIDRSYATFSHPEITPVVE-KDGVYILELFHGPTLAFKDVALQLLGNLFE 65 Query: 125 YISRKRGEVIHILGATSGDTGAAAIQGVRGKEGIKICILHPHGKVSKVQELQMTTVDDSN 184 Y+ ++RGE ++I+GATSGDTG+AAI GVRGK+ I I ILHPHGK S VQ LQMTTV D N Sbjct: 66 YLLKERGEKMNIVGATSGDTGSAAIYGVRGKDKINIFILHPHGKTSPVQALQMTTVLDPN 125 Query: 185 VLNLSVEGNFDDCQKVIKELFADLDFKGKYHLRAINSINFVRILAQTVYYFYAYLQIEDS 244 V N++ G FDDCQ ++K LF+DL FK KY L A+NSIN+ R+LAQ VYYFYAY ++ Sbjct: 126 VHNIAARGTFDDCQNIVKSLFSDLPFKEKYSLGAVNSINWARVLAQVVYYFYAYFRVAAL 185 Query: 245 DKKKINISVPSGNFGNIFSGYLAKKMGLPIHKLIIATNENNILERFVNTGEYKPGSFTST 304 +++ SVP+GNFG+IF+GY+AK+MGLPI +LI+ATNENNIL RF+N G+Y G +T Sbjct: 186 FGQEVVFSVPTGNFGDIFAGYVAKRMGLPIRRLILATNENNILSRFINGGDYSLGDVVAT 245 Query: 305 YSPSMDIQVASNFERYLYYLVGEDAEKLSAHMSKLQTEGKITIAGEALQQVQADFAALGV 364 SPSMDIQ+ASNFERY+YYL GE+ ++ + L T+G+I +++V+ +F + V Sbjct: 246 VSPSMDIQLASNFERYVYYLFGENPARVREAFAALPTKGRIDFTEAEMEKVRDEFLSRSV 305 Query: 365 KNQQCLDTIGKYNKEYDYLLDPHTACGIAAYEKFNGAGETAITFATAHPAKFDEAIA-LV 423 + + TI +++E Y+LDPHTA G+ A + G A+ ATAHPAKF EA+ V Sbjct: 306 NEDETIATIAAFHRETGYILDPHTAVGVKAALELVQDGTPAVCLATAHPAKFAEAVVRAV 365 Query: 424 DIRQEFPEQIAALFSLPQHQTVVKNDKDVIVRELQAFYE 462 P + + +LP V+ D+D I +AF E Sbjct: 366 GFEPSRPTSLEGIEALPSRCDVLDADRDAI----KAFIE 400 >gb|EAK39578.1| unknown [environmental sequence] Length = 462 Score = 404 bits (1038), Expect = e-111, Method: Compositional matrix adjust. Identities = 206/452 (45%), Positives = 290/452 (64%), Gaps = 5/452 (1%) Query: 1 MEYISTRGNVEAKGFIDTVLMGLADDGGLMVPFRIPVISPEKLAEWRSLSFQELFLEIFS 60 M+YISTRG + F +L GLA DGGL VP ++P +S E++ SLS+ +L L++ + Sbjct: 1 MKYISTRGRCSPQNFEQVLLTGLAPDGGLFVPKKLPTVSVEQIRAMGSLSYADLALDLVT 60 Query: 61 YYTNDEIPYEDLKEMVYTSYANFRAPEVTPLHQVNDSLYVLELFHGPTFAFKDVALQFMG 120 + + EIP DLK ++ SY F PEV P+ Q+++ Y+LEL+HGPT AFKD ALQ +G Sbjct: 61 PFVDGEIPTADLKTIIDRSYEAFDHPEVAPMTQLSEREYLLELYHGPTLAFKDFALQVLG 120 Query: 121 ELYSYISRKRGEVIHILGATSGDTGAAAIQGVRGKEGIKICILHPHGKVSKVQELQMTTV 180 L Y+ + + + ILGATSGDTG+AA++G R E ++I ILHP+ +VS VQ QMTTV Sbjct: 121 RLLDYVLERNDQRVVILGATSGDTGSAALEGCRHSERVEIYILHPYQRVSDVQRKQMTTV 180 Query: 181 DDSNVLNLSVEGNFDDCQKVIKELFADLDF-KGKYHLRAINSINFVRILAQTVYYFYAYL 239 NV NL++EGNFDDCQ+++K FAD F L A+NSIN+ RIL Q VYYFY+ L Sbjct: 181 SGKNVFNLAIEGNFDDCQRIVKAAFADQSFLPNDKSLVAVNSINWARILMQIVYYFYSAL 240 Query: 240 QIEDSDKKKINISVPSGNFGNIFSGYLAKKMGLPIHKLIIATNENNILERFVNTGEYKPG 299 ++ D K+++ SVP+GNFG+I++GYLAK MGLPI +LI+ATN+N+IL RF++ EY Sbjct: 241 KLGAPD-KRVSFSVPTGNFGDIYAGYLAKCMGLPIDRLIVATNKNDILHRFISANEYAKS 299 Query: 300 SFTSTYSPSMDIQVASNFERYLYYLVGEDAEKLSAHMSKLQTEGKITIAGEALQQVQADF 359 T T+SPSMDI V+SNFERYLY L +D +S M E +++ + Q+ F Sbjct: 300 QLTKTFSPSMDILVSSNFERYLYDLFAKDPSAVSEFMQH-SGEKPLSVTSQQWAQMNECF 358 Query: 360 AALGVKNQQCLDTIGKYNKEYDYLLDPHTACGI-AAYEKFNGAGETAITFATAHPAKFDE 418 + V + I + + L+DPHTA G+ + + F + +T ATAHPAKF E Sbjct: 359 DSSSVDDNTTCQVINELFNKDSVLVDPHTAVGVQSGRDCFENSRSPIVTLATAHPAKFSE 418 Query: 419 AIALVDI-RQEFPEQIAALFSLPQHQTVVKND 449 AI + + + PE + LFS + T++ ND Sbjct: 419 AIQAAGLNKPKLPEHLKDLFSREERYTILHND 450 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.137 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 694,250,388 Number of extensions: 29948994 Number of successful extensions: 75504 Number of sequences better than 1.0e-05: 642 Number of HSP's gapped: 74169 Number of HSP's successfully gapped: 642 Length of query: 462 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 333 Effective length of database: 406,989,807 Effective search space: 135527605731 Effective search space used: 135527605731 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)