BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0007.p (354 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_562186.1| probable ABC transporter [Clostridium perfringe... 360 4e-98 ref|NP_814947.1| spermidine/putrescine ABC transporter, ATP-bind... 354 1e-96 ref|ZP_00069732.1| COG3842: ABC-type spermidine/putrescine trans... 309 7e-83 gb|EAK29514.1| unknown [environmental sequence] 301 2e-80 ref|ZP_00060690.1| COG3842: ABC-type spermidine/putrescine trans... 276 4e-73 >ref|NP_562186.1| probable ABC transporter [Clostridium perfringens] dbj|BAB80976.1| probable ABC transporter [Clostridium perfringens str. 13] Length = 343 Score = 360 bits (923), Expect = 4e-98, Method: Compositional matrix adjust. Identities = 177/360 (49%), Positives = 252/360 (70%), Gaps = 24/360 (6%) Query: 1 MALLSLEHVSVAYDNRL-ILEDFNXXXXXXXXXXXXXPSGCGKTTTLRLIAGFLSAEAGN 59 M L++L++++V+YD + ILE+ N PSGCGKTTTLR++AGF+ AG Sbjct: 1 MDLINLQNINVSYDKKNNILENLNLKVKKGELVSLLGPSGCGKTTTLRVVAGFIEPTAGK 60 Query: 60 FMFSGKDYTKVPVEKRNFGFVFQNYALFPHLSVYDNVAFGLRLRKLKEAEVQKRVTRMLE 119 F+ +DYT +PV RNFG VFQ+YALFPHL+V +NVAFGL+++K+ + E++K+V +L+ Sbjct: 61 FLLGDEDYTNIPVHNRNFGLVFQSYALFPHLTVEENVAFGLKMKKMSKEEIRKKVDDILK 120 Query: 120 IVSLGGFEKRLPAALSGGQRQRVAIARALVIEPDLLLFDEPLSNLDANLRVNMRVEIRRI 179 +V + KR P LSGGQRQRVAIARALVIEP LLL DEPLSNLDA LR+ MRVEIR++ Sbjct: 121 VVDMSHLAKRYPKELSGGQRQRVAIARALVIEPSLLLLDEPLSNLDAKLRLKMRVEIRKL 180 Query: 180 QQELGITTVYVSHDQEECFSISDQVAVMNNGKIEQLDQPQAIFKYPASEFVARFIGFGNF 239 QQ+LGITT++V+HDQEECFSISD+VAV+N G IEQ D P+ I+ PA+EFVARF+GF NF Sbjct: 181 QQKLGITTLFVTHDQEECFSISDKVAVLNKGVIEQFDTPENIYSNPATEFVARFVGFENF 240 Query: 240 ISF----DG--RNDEGTDIELSRGTLKFLAVKRPNGEGNTGLLGAIRPDDLQLLSSPPES 293 I DG +N+ G ++++++ VK N + IRPDD++++ E Sbjct: 241 IDLIKVSDGIYKNESGINLKVNK-------VKDKN-----EVKATIRPDDIKIVKEDLE- 287 Query: 294 GATGNTLPGTVQVSTYLGRSYQYQVETELGVFTVNQELEHPFGQGEKVTVYFPKEKLVLV 353 NT+ G ++V T+LG++YQY ++T+LG VN E + + +G+ + + P +K+V++ Sbjct: 288 ----NTVNGVIEVRTFLGKAYQYDIKTKLGNLIVNSENDSIYEKGQNIKLQLPVDKIVIL 343 >ref|NP_814947.1| spermidine/putrescine ABC transporter, ATP-binding protein [Enterococcus faecalis V583] gb|AAO81017.1| spermidine/putrescine ABC transporter, ATP-binding protein [Enterococcus faecalis V583] Length = 346 Score = 354 bits (909), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 182/356 (51%), Positives = 237/356 (66%), Gaps = 13/356 (3%) Query: 1 MALLSLEHVSVAYDNRL-ILEDFNXXXXXXXXXXXXXPSGCGKTTTLRLIAGFLSAEAGN 59 M + L+ + V+YD + IL+D N PSGCGKTTTLR+IAG + G Sbjct: 1 MTFVDLKDIRVSYDGKQDILKDLNISMEKGELVSLLGPSGCGKTTTLRVIAGLIKPNDGQ 60 Query: 60 FMFSGKDYTKVPVEKRNFGFVFQNYALFPHLSVYDNVAFGLRLRKLKEAEVQKRVTRMLE 119 F ++ TKVPV KRNFG VFQ+YALFPHL++ +NVAFGL+LRK + + ++VT ML+ Sbjct: 61 FFVDQEELTKVPVHKRNFGMVFQSYALFPHLTIAENVAFGLKLRKENKQTMDQKVTEMLK 120 Query: 120 IVSLGGFEKRLPAALSGGQRQRVAIARALVIEPDLLLFDEPLSNLDANLRVNMRVEIRRI 179 + L R P LSGGQRQRVA+ARAL+IEP LLL DEPLSNLDA LRV MR+EI+RI Sbjct: 121 VCGLESLGDRYPKQLSGGQRQRVALARALIIEPKLLLLDEPLSNLDAKLRVAMRIEIKRI 180 Query: 180 QQELGITTVYVSHDQEECFSISDQVAVMNNGKIEQLDQPQAIFKYPASEFVARFIGFGNF 239 QQ+LGITTV+V+HDQEECFSISD+VA+MNNG IEQ D P+ I++ P ++FVA FIGF NF Sbjct: 181 QQQLGITTVFVTHDQEECFSISDKVAIMNNGVIEQYDSPETIYRLPRTKFVAEFIGFENF 240 Query: 240 ISFDGRNDEGTDIELSRGTLKFLAVKRPNGEGN-TGLLGAIRPDDLQLLSSPPESGATGN 298 S + + ++ VK N E N T + IRP+D++++S A N Sbjct: 241 FSVEKMEAGHYRTQTNQ------IVKTTNPEPNVTTTVATIRPEDIEIVSE-----AATN 289 Query: 299 TLPGTVQVSTYLGRSYQYQVETELGVFTVNQELEHPFGQGEKVTVYFPKEKLVLVQ 354 T+ GTV V T+LG+SYQY+VET LG VN E + E + + FP EKLV+++ Sbjct: 290 TVAGTVAVRTFLGKSYQYEVETALGTLLVNGTSEQLYETNETIHLAFPAEKLVILE 345 >ref|ZP_00069732.1| COG3842: ABC-type spermidine/putrescine transport systems, ATPase components [Oenococcus oeni MCW] Length = 350 Score = 309 bits (791), Expect = 7e-83, Method: Compositional matrix adjust. Identities = 161/353 (45%), Positives = 222/353 (62%), Gaps = 9/353 (2%) Query: 1 MALLSLEHVSVAYDNR-LILEDFNXXXXXXXXXXXXXPSGCGKTTTLRLIAGFLSAEAGN 59 MA L ++H++V+Y+ + IL D N PSGCGKTTTLR IAG + + G Sbjct: 1 MARLDVKHLAVSYNGKNKILNDINLTIEDGELVSLLGPSGCGKTTTLRSIAGLIRSNQGQ 60 Query: 60 FMFSGKDYTKVPVEKRNFGFVFQNYALFPHLSVYDNVAFGLRLRKLKEAEVQKRVTRMLE 119 + GKD +++PV KRNFG VFQ+YALFP L+V +NVAFGL+ + ++E ++ RV ML+ Sbjct: 61 ILVDGKDISRIPVYKRNFGMVFQSYALFPQLTVRENVAFGLKNQHIREPDLSSRVNEMLD 120 Query: 120 IVSLGGFEKRLPAALSGGQRQRVAIARALVIEPDLLLFDEPLSNLDANLRVNMRVEIRRI 179 IV L + R P+ LSGGQ+QRV++AR+LV P LLL DEPLSNLDA LRV MR IR I Sbjct: 121 IVGLLDYAARFPSQLSGGQQQRVSLARSLVTHPSLLLLDEPLSNLDAKLRVEMREMIREI 180 Query: 180 QQELGITTVYVSHDQEECFSISDQVAVMNNGKIEQLDQPQAIFKYPASEFVARFIGFGNF 239 QQ L +T ++V+HDQ ECF+ISD VAVMN G IEQ D ++I+ +P +EFVA FIG+ NF Sbjct: 181 QQRLKLTMLFVTHDQSECFAISDHVAVMNKGVIEQFDSSESIYHHPLTEFVAHFIGYENF 240 Query: 240 ISFDGRNDEGTDIELSRGTLKFLAV-KRPNGEGNTGLLGAIRPDDLQLLSSPPESGATGN 298 F + L L+F A PN T IRP+D++++ E+ N Sbjct: 241 --FQVKKFLKNQKYLLDHNLEFTAADSSPNVISLT-----IRPEDIEIIEKETENSDNHN 293 Query: 299 TLPGTVQVSTYLGRSYQYQVETELGVFTVNQELEHPFGQGEKVTVYFPKEKLV 351 L G + STYLG SY+YQ++T LG+ + + G+ + + FP +K++ Sbjct: 294 QLEGKILFSTYLGDSYRYQIDTPLGIVIAVSGTDKKYLSGQPIVLNFPSQKVL 346 >gb|EAK29514.1| unknown [environmental sequence] Length = 349 Score = 301 bits (770), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 159/364 (43%), Positives = 232/364 (63%), Gaps = 26/364 (7%) Query: 1 MALLSLEHVSVAYDNRLILEDFNXXXXXXXXXXXXXPSGCGKTTTLRLIAGFLSAEAGNF 60 M+ L L++++ +YD ++L + + SGCGKTTTLR+++GFL +G+ Sbjct: 1 MSYLQLKNLTASYDKEIVLNNLSLDIKKGELLSLLGSSGCGKTTTLRIVSGFLKPISGSV 60 Query: 61 MFSGKDYTKVPVEKRNFGFVFQNYALFPHLSVYDNVAFGLRLRKLKEAEVQKRVTRMLEI 120 + D + P +R FG+VFQ+YALFPHL+V DNV FGL+ RK++ +++ +V ML++ Sbjct: 61 ILDDTDISLKPPNERGFGYVFQSYALFPHLTVEDNVGFGLKQRKVRSDQIKVKVDEMLDL 120 Query: 121 VSLGGFEKRLPAALSGGQRQRVAIARALVIEPDLLLFDEPLSNLDANLRVNMRVEIRRIQ 180 V L F R P LSGGQ+QRVA+ARA+ I+P LLL DEPLSNLDA LR+ MR+EI+RIQ Sbjct: 121 VDLSDFRSRRPKELSGGQKQRVALARAMAIDPGLLLLDEPLSNLDAALRIKMRIEIKRIQ 180 Query: 181 QELGITTVYVSHDQEECFSISDQVAVMNNGKIEQLDQPQAIFKYPASEFVARFIGFGNFI 240 QE +T +YV+HDQEECFSISD+VA+MN+G IEQL +P+ I+ P ++FVA+F+GF NF Sbjct: 181 QEHAMTAIYVTHDQEECFSISDRVAIMNDGLIEQLAKPEEIYSSPKTQFVAKFVGFENF- 239 Query: 241 SFDGRNDEGTDIELSRGTLKFLAVKRPNGEGNTGLL-----------GAIRPDDLQLLSS 289 F G +I+ S G F++ K G T +L A RP+ ++LSS Sbjct: 240 -FKG------EIKDSSGKYHFISEK---GGYETPILDQTIIQRKTAIAAFRPNGFEILSS 289 Query: 290 PPESGATGNTLPGTVQVSTYLGRSYQYQVETELGVFTVNQELEHPFGQGEKVTVYFPKEK 349 + + +PG ++V TYLG+ Y+Y ++TELG V+ E+ F +V + E Sbjct: 290 DQKDQS---LIPGLIRVQTYLGKGYRYLIDTELGELVVDHT-ENKFSINTQVFLKPLPEM 345 Query: 350 LVLV 353 +VL+ Sbjct: 346 MVLL 349 >ref|ZP_00060690.1| COG3842: ABC-type spermidine/putrescine transport systems, ATPase components [Clostridium thermocellum ATCC 27405] Length = 367 Score = 276 bits (707), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 141/298 (47%), Positives = 199/298 (66%), Gaps = 14/298 (4%) Query: 37 PSGCGKTTTLRLIAGFLSAEAGNFMFSGKDYTKVPVEKRNFGFVFQNYALFPHLSVYDNV 96 PSGCGKTT LR+I GF S E G GK +P KRN G VFQNYA+FPH+SV DN+ Sbjct: 38 PSGCGKTTLLRMIIGFNSIEGGQIKIDGKVINNIPTNKRNMGMVFQNYAIFPHMSVKDNI 97 Query: 97 AFGLRLRKLKEAEVQKRVTRMLEIVSLGGFEKRLPAALSGGQRQRVAIARALVIEPDLLL 156 AFGL+ RK+ +AE++KRV +L+IV + + R+P +SGGQ+QR+A+ARA+VI P++LL Sbjct: 98 AFGLKNRKVPKAEIEKRVDEILKIVKIDHLKDRMPHKMSGGQQQRIALARAIVIHPEVLL 157 Query: 157 FDEPLSNLDANLRVNMRVEIRRIQQELGITTVYVSHDQEECFSISDQVAVMNNGKIEQLD 216 DEPLSNLDA LR MR I++IQQ++GITTVYV+HDQEE ++SD++AVMN G I+Q+D Sbjct: 158 MDEPLSNLDAKLRTEMRNAIKQIQQQVGITTVYVTHDQEEALAVSDRIAVMNGGVIQQID 217 Query: 217 QPQAIFKYPASEFVARFIGFGNFISFDGRNDEGTDIELSRGTLKFLA--VKRPNGEGNTG 274 P+ I++ PA+ FV+ FIG N + ++E + G KF +K+ G N Sbjct: 218 TPKNIYQRPANTFVSTFIGLSNIMDGKLESNENGKALIRIGDYKFQMNNIKKDCG-SNCP 276 Query: 275 LLGAIRPDDLQLLSSPPESGATGNTLPGTVQVSTYLGRSYQYQVETELGVFTVNQELE 332 + ++RP++ +++ E G +P TV+ S +LG + Y VET+ G QE+E Sbjct: 277 VKVSVRPEEF-IINKETEEG-----IPVTVKSSVFLGVTTHYFVETKEG-----QEIE 323 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.138 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 500,405,131 Number of extensions: 20947086 Number of successful extensions: 105322 Number of sequences better than 1.0e-05: 20187 Number of HSP's gapped: 85151 Number of HSP's successfully gapped: 21326 Length of query: 354 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 227 Effective length of database: 412,420,005 Effective search space: 93619341135 Effective search space used: 93619341135 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)