BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0003.p (198 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_562185.1| probable spermidine/putrescine-binding protein ... 141 1e-32 ref|NP_814948.1| spermidine/putrescine ABC transporter, spermidi... 130 2e-29 ref|ZP_00069731.1| COG0687: Spermidine/putrescine-binding peripl... 118 8e-26 ref|ZP_00030335.1| COG0687: Spermidine/putrescine-binding peripl... 97 3e-19 ref|NP_437317.1| putative spermidineputrescine ABC transporter p... 91 1e-17 >ref|NP_562185.1| probable spermidine/putrescine-binding protein 1 [Clostridium perfringens] dbj|BAB80975.1| probable spermidine/putrescine-binding protein 1 [Clostridium perfringens str. 13] Length = 348 Score = 141 bits (355), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 83/197 (42%), Positives = 113/197 (57%), Gaps = 4/197 (2%) Query: 1 VKSWSDMWTQFDKN-LTLPAITATAGPMIVDAASVAAGG--SEFNEDQAFTKLKELDSNV 57 + SW D+W KN + +P IT T GP +V+ A+ AG N + AF +L+ L NV Sbjct: 150 INSWEDLWKPELKNKIAIPDITTTNGPAMVEIAAEKAGVDVKTDNGEAAFKELEALKPNV 209 Query: 58 VKYYSQTSEYVNMFGQEEIAGGPIMEMYFKDLQAAVPDAKFVAPSEGAYAVMNTVNVVKG 117 VK YS++S+ NMF EI + F + A P+ V P G Y NT+N+ K Sbjct: 210 VKTYSKSSDLANMFSNGEIVAAVASDFAFGTISKAKPEVINVIPESGTYLNFNTININKN 269 Query: 118 SDNKELAEAFINWQLSKEVQEKSAKAKVDSPVNTEVVLTDEEAVGVTYGADVISKLRKLD 177 S NK+LA FIN+ LSKEVQEK+AKA +SPVN EV L EE +TYG V+ + +D Sbjct: 270 SKNKDLAYEFINYALSKEVQEKTAKALNESPVNKEVKLNGEETKNLTYGP-VVDNAKVID 328 Query: 178 MSFVNQHITEWTDRFNR 194 FVN + +W + +NR Sbjct: 329 FKFVNSVMDQWVNNWNR 345 >ref|NP_814948.1| spermidine/putrescine ABC transporter, spermidine/putrescine-binding protein [Enterococcus faecalis V583] gb|AAO81018.1| spermidine/putrescine ABC transporter, spermidine/putrescine-binding protein [Enterococcus faecalis V583] Length = 354 Score = 130 bits (326), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 69/197 (35%), Positives = 115/197 (58%), Gaps = 4/197 (2%) Query: 1 VKSWSDMWT-QFDKNLTLPAITATAGPMIVDAASVAAGG--SEFNEDQAFTKLKELDSNV 57 +K+W D+W+ +++P + TAGP+++ AS AG ++ N AF +KEL NV Sbjct: 155 IKNWDDLWSADLKGKISVPDVATTAGPLMLYVASEHAGQDITKDNGKAAFEAMKELKPNV 214 Query: 58 VKYYSQTSEYVNMFGQEEIAGGPIMEMYFKDLQAAVPDAKFVAPSEGAYAVMNTVNVVKG 117 VK YS++S+ NMF EI + + +Q A + ++ P G YA NTVN+ K Sbjct: 215 VKTYSKSSDLANMFQSGEIEAAVVADFAVDIIQGAAENVTYIVPESGTYANYNTVNIPKK 274 Query: 118 SDNKELAEAFINWQLSKEVQEKSAKAKVDSPVNTEVVLTDEEAVGVTYGADVISKLRKLD 177 + NKE A F+N ++S+E Q+ A + + P N +V L+++EA TYGA + + + +D Sbjct: 275 AQNKETAFKFVNARISEESQKAKAISLNEGPTNQQVTLSEKEAKNKTYGA-IAERAKTVD 333 Query: 178 MSFVNQHITEWTDRFNR 194 +F+N + +W D++NR Sbjct: 334 FNFINSQLADWIDQWNR 350 >ref|ZP_00069731.1| COG0687: Spermidine/putrescine-binding periplasmic protein [Oenococcus oeni MCW] Length = 354 Score = 118 bits (295), Expect = 8e-26, Method: Compositional matrix adjust. Identities = 67/201 (33%), Positives = 117/201 (58%), Gaps = 6/201 (2%) Query: 1 VKSWSDMWTQFDKN-LTLPAITATAGPMIVDAASVAAGGSEFNED--QAFTKLKELDSNV 57 + SW+ +W+ KN +++P +T T GP ++ A+ +G S ++ AF+ LK L N+ Sbjct: 153 ITSWNQLWSSKLKNKISIPDVTTTFGPAMLYIAADDSGKSITSKSGASAFSALKSLKPNL 212 Query: 58 VKYYSQTSEYVNMFGQEEIAGGPIMEMYFKDLQAAVPDAKFVAPSEGAYAVMNTVNVVKG 117 VK Y+Q+S+ NMF EI + + L+ + + ++V P+ G YA +TV++VKG Sbjct: 213 VKTYTQSSDLANMFNSGEIQAAVVGDYAVGMLETSNSNLRYVVPNSGTYANYDTVSIVKG 272 Query: 118 SDNKELAEAFINWQLSKEVQEKSAKAKV--DSPVNTEVVLTDEEAVGVTYGADVISKLRK 175 + N + A ++++++SK VQ+K AK ++PVNT+V L+ A TYG + + + Sbjct: 273 TKNLKAAYKYLDYRISKSVQKKVAKTSSLNNAPVNTKVKLSAANAKNKTYG-KIADRAKT 331 Query: 176 LDMSFVNQHITEWTDRFNREI 196 +D +VN H+ W R+N I Sbjct: 332 IDFKYVNAHLKNWITRWNSLI 352 >ref|ZP_00030335.1| COG0687: Spermidine/putrescine-binding periplasmic protein [Burkholderia fungorum] Length = 383 Score = 96.7 bits (239), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 69/200 (34%), Positives = 101/200 (50%), Gaps = 10/200 (5%) Query: 3 SWSDMW-TQFDKNLTLPAITATAGPMIVDAASVAAGGSEFNEDQAFTKL-KELDSNVVKY 60 SW + + L +P IT T G + + +GG E N D FT + K++ NV+ + Sbjct: 186 SWKALTDPRIKGKLGVPPITNTYGLHTLVMLARLSGGGEKNIDPGFTAMTKQVAPNVLSW 245 Query: 61 YSQTSEYVNMFGQEEIAGGPIMEMYFKDLQAAVPDA----KFVAPSEGAYAVMNTVNVVK 116 E M GQ + +G I+ Y A+ + KF+ P EGA A+ VV Sbjct: 246 APTPGE---MDGQMQ-SGDVILAPYGSGRAVALQNTGFPLKFIYPKEGAVALQVAACVVA 301 Query: 117 GSDNKELAEAFINWQLSKEVQEKSAKAKVDSPVNTEVVLTDEEAVGVTYGADVISKLRKL 176 + +L++ F+ + LS EVQ A+ PVN V LT E A V YG + ISKL + Sbjct: 302 ENAQPQLSQQFVQYLLSPEVQALQAQGIGLGPVNKTVKLTPEVAARVPYGPEQISKLTAM 361 Query: 177 DMSFVNQHITEWTDRFNREI 196 D + +NQH TEWT+R+NR + Sbjct: 362 DWTTINQHRTEWTERWNRSV 381 >ref|NP_437317.1| putative spermidineputrescine ABC transporter periplasmic solute-binding protein precursor [Sinorhizobium meliloti 1021] pir||A95939 probable spermidineputrescine ABC transporter periplasmic solute-binding protein precursor potD [imported] - Sinorhizobium meliloti (strain 1021) magaplasmid pSymB emb|CAC49177.1| putative spermidineputrescine ABC transporter periplasmic solute-binding protein precursor [Sinorhizobium meliloti] Length = 340 Score = 90.9 bits (224), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 57/198 (28%), Positives = 98/198 (49%), Gaps = 1/198 (0%) Query: 1 VKSWSDMW-TQFDKNLTLPAITATAGPMIVDAASVAAGGSEFNEDQAFTKLKELDSNVVK 59 V SW+D+ + ++ LP +T GP + A G + + A + E ++V Sbjct: 142 VTSWADLLGDKLAGHVALPNVTTNQGPPTLYMIGEALGQNTPDLKAAIAAVGEKKDDIVT 201 Query: 60 YYSQTSEYVNMFGQEEIAGGPIMEMYFKDLQAAVPDAKFVAPSEGAYAVMNTVNVVKGSD 119 +Y ++S+ V + QEEI PI ++ K+ P EG MN + + KGS Sbjct: 202 FYVKSSQLVQLMQQEEIWAAPIGRFSWEGFTKMDLPIKWATPKEGQTGGMNVMVLAKGSK 261 Query: 120 NKELAEAFINWQLSKEVQEKSAKAKVDSPVNTEVVLTDEEAVGVTYGADVISKLRKLDMS 179 N++LA F+++ LS E+Q A+ VDSP N EV ++ E A +TYG + + L+ + + Sbjct: 262 NQDLALQFMDFWLSTEIQTALAEKLVDSPANKEVKVSPEIAENLTYGEETVKNLKLIPSA 321 Query: 180 FVNQHITEWTDRFNREIG 197 + W +N ++G Sbjct: 322 VALDNREAWLSEWNAKVG 339 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.312 0.127 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 291,553,996 Number of extensions: 10955598 Number of successful extensions: 24262 Number of sequences better than 1.0e-05: 32 Number of HSP's gapped: 24269 Number of HSP's successfully gapped: 32 Length of query: 198 Length of database: 757,237,578 Length adjustment: 119 Effective length of query: 79 Effective length of database: 434,140,797 Effective search space: 34297122963 Effective search space used: 34297122963 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 123 (52.0 bits)