BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0002.p (206 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_829953.1| Peptidyl-tRNA hydrolase [Bacillus cereus ATCC 1... 216 2e-55 ref|NP_976377.1| peptidyl-tRNA hydrolase [Bacillus cereus ATCC 1... 216 3e-55 ref|NP_653999.1| Pept_tRNA_hydro, Peptidyl-tRNA hydrolase [Bacil... 215 6e-55 sp|Q81J96|PTH_BACCR Peptidyl-tRNA hydrolase (PTH) 213 2e-54 ref|NP_842619.1| peptidyl-tRNA hydrolase [Bacillus anthracis str... 213 3e-54 >ref|NP_829953.1| Peptidyl-tRNA hydrolase [Bacillus cereus ATCC 14579] gb|AAP07154.1| Peptidyl-tRNA hydrolase [Bacillus cereus ATCC 14579] Length = 210 Score = 216 bits (550), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 104/199 (52%), Positives = 135/199 (67%), Gaps = 4/199 (2%) Query: 7 VSAEETSVERIAPLMKWIIGLGNPGPQYAKTKHNVGFMALDELAARHGIVFNQNKCKSVI 66 VS +E+S R MK I+GLGNPG +Y T+HN+GFMA+DELA R I N+ K K V Sbjct: 15 VSKKESSGTR----MKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGVF 70 Query: 67 GEGVIDGVKTVLIKPMTFMNLSGEAVRAYMDYYKVQLEDMIVVYDDLDTELGKIRLRYQG 126 G G ++G K +L+KP+T+MNLSGE++R MDYYK+ +ED +V+YDDLD +GK+RLR +G Sbjct: 71 GAGFVNGEKVILLKPLTYMNLSGESIRPLMDYYKIDVEDFVVMYDDLDIPVGKLRLRMKG 130 Query: 127 SAGGHNGIKSIIQHTGTQSFNRVRMGISRPEPGFAIVDYVLSTFPKKDGDKLEKMIQDTG 186 SAGGHNG+KS I H GTQ F R+RMGI RP+ G +VDYVL F ++ + I+ Sbjct: 131 SAGGHNGVKSTISHLGTQEFQRIRMGIDRPKNGMKVVDYVLGRFTSEEIPDVNHSIEKAA 190 Query: 187 SALEFSLKHTFEQTMAKFN 205 A E L F Q M FN Sbjct: 191 DACEEWLNKPFLQIMNTFN 209 >ref|NP_976377.1| peptidyl-tRNA hydrolase [Bacillus cereus ATCC 10987] gb|AAS38985.1| peptidyl-tRNA hydrolase [Bacillus cereus ATCC 10987] Length = 210 Score = 216 bits (549), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 104/199 (52%), Positives = 135/199 (67%), Gaps = 4/199 (2%) Query: 7 VSAEETSVERIAPLMKWIIGLGNPGPQYAKTKHNVGFMALDELAARHGIVFNQNKCKSVI 66 VS +E+S R MK I+GLGNPG +Y T+HN+GFMA+DELA R I N+ K K V Sbjct: 15 VSKKESSGTR----MKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGVF 70 Query: 67 GEGVIDGVKTVLIKPMTFMNLSGEAVRAYMDYYKVQLEDMIVVYDDLDTELGKIRLRYQG 126 G G ++G K +L+KP+T+MNLSGE++R MDYYK+ +ED +V+YDDLD +GK+RLR +G Sbjct: 71 GAGFVNGEKVILLKPLTYMNLSGESIRPLMDYYKIDVEDFVVLYDDLDIPVGKLRLRMKG 130 Query: 127 SAGGHNGIKSIIQHTGTQSFNRVRMGISRPEPGFAIVDYVLSTFPKKDGDKLEKMIQDTG 186 SAGGHNG+KS I H GTQ F R+RMGI RP+ G +VDYVL F ++ + I+ Sbjct: 131 SAGGHNGVKSTISHLGTQEFQRIRMGIDRPKNGMKVVDYVLGRFTSEEIPGVNHSIEKAA 190 Query: 187 SALEFSLKHTFEQTMAKFN 205 A E L F Q M FN Sbjct: 191 DACEEWLNKPFLQIMNTFN 209 >ref|NP_653999.1| Pept_tRNA_hydro, Peptidyl-tRNA hydrolase [Bacillus anthracis A2012] Length = 207 Score = 215 bits (547), Expect = 6e-55, Method: Compositional matrix adjust. Identities = 103/199 (51%), Positives = 135/199 (67%), Gaps = 4/199 (2%) Query: 7 VSAEETSVERIAPLMKWIIGLGNPGPQYAKTKHNVGFMALDELAARHGIVFNQNKCKSVI 66 VS +E+S R MK I+GLGNPG +Y T+HN+GFMA+DELA R I N+ K K + Sbjct: 12 VSKKESSGTR----MKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGLF 67 Query: 67 GEGVIDGVKTVLIKPMTFMNLSGEAVRAYMDYYKVQLEDMIVVYDDLDTELGKIRLRYQG 126 G G ++G K +L+KP+T+MNLSGE++R MDYYK+ +ED +V+YDDLD +GK+RLR +G Sbjct: 68 GAGFVNGEKVILLKPLTYMNLSGESIRPLMDYYKIDVEDFVVLYDDLDIPVGKLRLRMKG 127 Query: 127 SAGGHNGIKSIIQHTGTQSFNRVRMGISRPEPGFAIVDYVLSTFPKKDGDKLEKMIQDTG 186 SAGGHNG+KS I H GTQ F R+RMGI RP+ G +VDYVL F ++ + I+ Sbjct: 128 SAGGHNGVKSTISHLGTQEFQRIRMGIDRPKNGMKVVDYVLGRFTSEEIPDVNHSIEKAA 187 Query: 187 SALEFSLKHTFEQTMAKFN 205 A E L F Q M FN Sbjct: 188 DACEEWLNKPFLQIMNTFN 206 >sp|Q81J96|PTH_BACCR Peptidyl-tRNA hydrolase (PTH) Length = 186 Score = 213 bits (543), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 99/185 (53%), Positives = 128/185 (69%) Query: 21 MKWIIGLGNPGPQYAKTKHNVGFMALDELAARHGIVFNQNKCKSVIGEGVIDGVKTVLIK 80 MK I+GLGNPG +Y T+HN+GFMA+DELA R I N+ K K V G G ++G K +L+K Sbjct: 1 MKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGVFGAGFVNGEKVILLK 60 Query: 81 PMTFMNLSGEAVRAYMDYYKVQLEDMIVVYDDLDTELGKIRLRYQGSAGGHNGIKSIIQH 140 P+T+MNLSGE++R MDYYK+ +ED +V+YDDLD +GK+RLR +GSAGGHNG+KS I H Sbjct: 61 PLTYMNLSGESIRPLMDYYKIDVEDFVVMYDDLDIPVGKLRLRMKGSAGGHNGVKSTISH 120 Query: 141 TGTQSFNRVRMGISRPEPGFAIVDYVLSTFPKKDGDKLEKMIQDTGSALEFSLKHTFEQT 200 GTQ F R+RMGI RP+ G +VDYVL F ++ + I+ A E L F Q Sbjct: 121 LGTQEFQRIRMGIDRPKNGMKVVDYVLGRFTSEEIPDVNHSIEKAADACEEWLNKPFLQI 180 Query: 201 MAKFN 205 M FN Sbjct: 181 MNTFN 185 >ref|NP_842619.1| peptidyl-tRNA hydrolase [Bacillus anthracis str. Ames] sp|Q81VY9|PTH_BACAA Peptidyl-tRNA hydrolase (PTH) gb|AAP24105.1| peptidyl-tRNA hydrolase [Bacillus anthracis str. Ames] Length = 186 Score = 213 bits (541), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 98/185 (52%), Positives = 128/185 (69%) Query: 21 MKWIIGLGNPGPQYAKTKHNVGFMALDELAARHGIVFNQNKCKSVIGEGVIDGVKTVLIK 80 MK I+GLGNPG +Y T+HN+GFMA+DELA R I N+ K K + G G ++G K +L+K Sbjct: 1 MKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGLFGAGFVNGEKVILLK 60 Query: 81 PMTFMNLSGEAVRAYMDYYKVQLEDMIVVYDDLDTELGKIRLRYQGSAGGHNGIKSIIQH 140 P+T+MNLSGE++R MDYYK+ +ED +V+YDDLD +GK+RLR +GSAGGHNG+KS I H Sbjct: 61 PLTYMNLSGESIRPLMDYYKIDVEDFVVLYDDLDIPVGKLRLRMKGSAGGHNGVKSTISH 120 Query: 141 TGTQSFNRVRMGISRPEPGFAIVDYVLSTFPKKDGDKLEKMIQDTGSALEFSLKHTFEQT 200 GTQ F R+RMGI RP+ G +VDYVL F ++ + I+ A E L F Q Sbjct: 121 LGTQEFQRIRMGIDRPKNGMKVVDYVLGRFTSEEIPDVNHSIEKAADACEEWLNKPFLQI 180 Query: 201 MAKFN 205 M FN Sbjct: 181 MNTFN 185 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.137 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 312,653,913 Number of extensions: 12830540 Number of successful extensions: 29176 Number of sequences better than 1.0e-05: 617 Number of HSP's gapped: 28579 Number of HSP's successfully gapped: 617 Length of query: 206 Length of database: 757,237,578 Length adjustment: 120 Effective length of query: 86 Effective length of database: 431,425,698 Effective search space: 37102610028 Effective search space used: 37102610028 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 123 (52.0 bits)