BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0016.p (326 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|P55785|HEP2_BACST Heptaprenyl diphosphate synthase component ... 218 1e-55 ref|NP_390155.1| heptaprenyl diphosphate synthase component II [... 206 5e-52 ref|NP_977962.1| heptaprenyl diphosphate synthase component II [... 192 7e-48 gb|AAN87493.1| Farnesyl pyrophosphate synthetase [Heliobacillus ... 191 2e-47 ref|NP_646176.1| heptaprenyl diphosphate syntase component II [S... 189 8e-47 >sp|P55785|HEP2_BACST Heptaprenyl diphosphate synthase component II (HEPPP synthase subunit 2) dbj|BAA08725.1| component II of heptaprenyl diphosphate synthase [Geobacillus stearothermophilus] Length = 320 Score = 218 bits (556), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 123/323 (38%), Positives = 188/323 (58%), Gaps = 9/323 (2%) Query: 3 LHEALNIDLDEINHEIQNIVARDKDVPKKSLLAQSILELTSSGGKRLRPLMVIVGSRFGR 62 ++ L+ DL + E++ V + L ++ L L +GGKR+RP+ V++ +RFG+ Sbjct: 6 MYSFLSDDLAAVEEELERAVQSEY-----GPLGEAALHLLQAGGKRIRPVFVLLAARFGQ 60 Query: 63 KPSTRRTLQLSAAAEFIHAASLIHDDIIDDAELRRGVPALHTKTGVLSAVHIGNYMSARV 122 R ++ A E IH ASL+HDD+IDDA+LRRG P + K A++ G+Y+ AR Sbjct: 61 Y-DLERMKHVAVALELIHMASLVHDDVIDDADLRRGRPTIKAKWSNRFAMYTGDYLFARS 119 Query: 123 IELLSKYTGDKNRYVHDLSSVATAQLCLGEYQQMEHAYDYNLTFDEYLEKSRNKTAMLMA 182 +E +++ N H + + ++C GE +Q++ Y ++ YL + R KTA+L+A Sbjct: 120 LERMAELG---NPRAHQVLAKTIVEVCRGEIEQIKDKYRFDQPLRTYLRRIRRKTALLIA 176 Query: 183 TCLRVGALSAESPEEVAGLLYDFGEALGMSFQIQDDLLDFTQSADVLGKPAGSDLRNGQV 242 ++GAL+A +PE + LY FG +GMSFQI DD+LDFT + + LGKPAGSDL G V Sbjct: 177 ASCQLGALAAGAPEPIVKRLYWFGHYVGMSFQITDDILDFTGTEEQLGKPAGSDLLQGNV 236 Query: 243 TLPVLFALQDPELAPVIRTIGPASLEEEVAHVLALINRSDALFRTEEVSQDYLGQAAAII 302 TLPVL+AL D + I +GP + E+A V++ I R+DA+ R+ +S YL +A ++ Sbjct: 237 TLPVLYALSDERVKAAIAAVGPETDVAEMAAVISAIKRTDAIERSYALSDRYLDKALHLL 296 Query: 303 QRLSSYPAHADLETLLQYFAGRD 325 L A L L Y RD Sbjct: 297 DGLPMNEARGLLRDLALYIGKRD 319 >ref|NP_390155.1| heptaprenyl diphosphate synthase component II [Bacillus subtilis] sp|P31114|HEP2_BACSU Heptaprenyl diphosphate synthase component II (HEPPP synthase subunit 2) (Spore germination protein C3) pir||E69630 heptaprenyl diphosphate synthase component II gerCC - Bacillus subtilis gb|AAA20856.1| GerC3 [Bacillus subtilis] emb|CAB14190.1| heptaprenyl diphosphate synthase component II [Bacillus subtilis subsp. subtilis str. 168] Length = 348 Score = 206 bits (524), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 116/321 (36%), Positives = 188/321 (58%), Gaps = 15/321 (4%) Query: 7 LNIDLDEINHEIQNIVARDKDVPKKSLLAQSILELTSSGGKRLRPLMVIVGSRFGRKPST 66 LN D+D I E++ V D LL+++ L L +GGKR+RP+ V++ FG Sbjct: 38 LNDDIDVIERELEQTVRSDY-----PLLSEAGLHLLQAGGKRIRPVFVLLSGMFG-DYDI 91 Query: 67 RRTLQLSAAAEFIHAASLIHDDIIDDAELRRGVPALHTKTGVLSAVHIGNYMSARVIELL 126 + ++ E IH ASL+HDD+IDDAELRRG P + K A++ G+YM A +E++ Sbjct: 92 NKIKYVAVTLEMIHMASLVHDDVIDDAELRRGKPTIKAKWDNRIAMYTGDYMLAGSLEMM 151 Query: 127 SKYTGDKNRYVHDLSSVATAQLCLGEYQQMEHAYDYNLTFDEYLEKSRNKTAMLMATCLR 186 ++ K H + S ++CLGE +Q++ Y+ YL + + KTA+L+A + Sbjct: 152 TRINEPK---AHRILSQTIVEVCLGEIEQIKDKYNMEQNLRTYLRRIKRKTALLIAVSCQ 208 Query: 187 VGALSAESPEEVAGLLYDFGEALGMSFQIQDDLLDFTQSADVLGKPAGSDLRNGQVTLPV 246 +GA+++ + E++ LY FG +GMS+QI DD+LDFT + + LGKP G DL G VTLPV Sbjct: 209 LGAIASGADEKIHKALYWFGYYVGMSYQIIDDILDFTSTEEELGKPVGGDLLQGNVTLPV 268 Query: 247 LFALQDPELAPVIRTIGPASLEEEVAHVLALINRSDALFRTEEVSQDYLGQAAAIIQRLS 306 L+AL++P L ++ I + +E++ ++ I ++DA+ + VS+ YL +A Q+L+ Sbjct: 269 LYALKNPALKNQLKLINSETTQEQLEPIIEEIKKTDAIEASMAVSEMYLQKA---FQKLN 325 Query: 307 SYP---AHADLETLLQYFAGR 324 + P A + L + +Y R Sbjct: 326 TLPRGRARSSLAAIAKYIGKR 346 >ref|NP_977962.1| heptaprenyl diphosphate synthase component II [Bacillus cereus ATCC 10987] gb|AAS40570.1| heptaprenyl diphosphate synthase component II [Bacillus cereus ATCC 10987] Length = 320 Score = 192 bits (489), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 107/322 (33%), Positives = 183/322 (56%), Gaps = 9/322 (2%) Query: 3 LHEALNIDLDEINHEIQNIVARDKDVPKKSLLAQSILELTSSGGKRLRPLMVIVGSRFGR 62 ++ L D++ I E++ VA ++ L+ ++ L+L +GGKR+RP+ V++ +FG Sbjct: 6 MYSFLRSDINVIEKELKKTVASEQ-----PLVEEAALQLIEAGGKRIRPVFVLLAGKFG- 59 Query: 63 KPSTRRTLQLSAAAEFIHAASLIHDDIIDDAELRRGVPALHTKTGVLSAVHIGNYMSARV 122 ++ A E IH ASL+HDD+ID A LRRG ++ K G A++ G+Y+ A+ Sbjct: 60 DYKLDAIKHVAVALELIHMASLVHDDVIDAAFLRRGSATVNAKWGDRIAMYTGDYLFAKS 119 Query: 123 IELLSKYTGDKNRYVHDLSSVATAQLCLGEYQQMEHAYDYNLTFDEYLEKSRNKTAMLMA 182 +E + T + H S ++C GE +Q++ YDY+ YL + + KTA+L+A Sbjct: 120 LECI---TNIEIPEAHQALSHTILEVCKGEIEQIKDKYDYDQNLRTYLRRIKRKTALLIA 176 Query: 183 TCLRVGALSAESPEEVAGLLYDFGEALGMSFQIQDDLLDFTQSADVLGKPAGSDLRNGQV 242 ++GA++A + + L+ +G +GMS+QI DD+LDF + + LGKPAG DL G + Sbjct: 177 ASCQLGAIAAGANRDTVSRLFWYGYFVGMSYQIIDDILDFVSTEEKLGKPAGGDLLQGNI 236 Query: 243 TLPVLFALQDPELAPVIRTIGPASLEEEVAHVLALINRSDALFRTEEVSQDYLGQAAAII 302 TLP L+A++DP L I+++ + +E+ ++ + SDA+ + S+ YL +A II Sbjct: 237 TLPALYAMEDPVLCKKIKSVHENTTADEMKEIIEAVKNSDAIDKAFAFSERYLHKALEII 296 Query: 303 QRLSSYPAHADLETLLQYFAGR 324 + L A L+ + +Y R Sbjct: 297 KPLPRGQAKYALQNVAKYIGKR 318 >gb|AAN87493.1| Farnesyl pyrophosphate synthetase [Heliobacillus mobilis] Length = 320 Score = 191 bits (485), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 101/286 (35%), Positives = 171/286 (59%), Gaps = 7/286 (2%) Query: 41 LTSSGGKRLRPLMVIVGSRFGRKPSTRRTLQLSAAAEFIHAASLIHDDIIDDAELRRGVP 100 L +GGKRLRP V++ RF S R L L+ A E IH ASL+HDD++D+++ RRG+P Sbjct: 38 LLHAGGKRLRPAFVLLAGRF-HNYSLERLLPLAVAMEMIHMASLVHDDVVDESKTRRGIP 96 Query: 101 ALHTKTGVLSAVHIGNYMSARVIELLSKYTGDKNRYVHDLSSVATAQLCLGEYQQMEHAY 160 + + G ++H G+++ AR + L+++ DK + ++ + + ++C GE QQM + Sbjct: 97 TVKARWGNRISIHTGDHLFARSLLLIAELNDDK---ISEVLAKVSVEMCKGEIQQMSGTF 153 Query: 161 DYNLTFDEYLEKSRNKTAMLMATCLRVGALSAESPEEVAGLLYDFGEALGMSFQIQDDLL 220 D N TF +YL + KTA+L++ ++GA++ ++ + L +G LGM+FQ+ DD+L Sbjct: 154 DVNQTFRDYLFHIKRKTALLISASCQLGAMAVKADPSIVRALKMYGHHLGMAFQVTDDIL 213 Query: 221 DFTQSADVLGKPAGSDLRNGQVTLPVLFALQDPELAPVIRTIGPA--SLEEEVAHVLALI 278 D T LGKP GSDLR G +TLP+L AL + + P++R + +EEV + +I Sbjct: 214 DLTADEAELGKPVGSDLRQGIITLPILEALWETK-DPLLRLLVAKRDKSQEEVGQAIEII 272 Query: 279 NRSDALFRTEEVSQDYLGQAAAIIQRLSSYPAHADLETLLQYFAGR 324 S A+ R++++++ YL +A ++RL P L ++ ++ R Sbjct: 273 KNSGAIDRSQKIARRYLEKAKDELKRLPPVPTRDSLASIAEFIEKR 318 >ref|NP_646176.1| heptaprenyl diphosphate syntase component II [Staphylococcus aureus subsp. aureus MW2] dbj|BAB95224.1| heptaprenyl diphosphate syntase component II [Staphylococcus aureus subsp. aureus MW2] Length = 319 Score = 189 bits (480), Expect = 8e-47, Method: Compositional matrix adjust. Identities = 114/316 (36%), Positives = 178/316 (56%), Gaps = 7/316 (2%) Query: 14 INHEIQNIVAR-DKDVPKK-SLLAQSILELTSSGGKRLRPLMVIVGSRFGRKPST-RRTL 70 +N+EI+ + R +K + K S+L Q+ L L SSGGKR+RP VI+ S+FG+ T +T Sbjct: 6 MNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVRPAFVILSSQFGKDEQTSEQTY 65 Query: 71 QLSAAAEFIHAASLIHDDIIDDAELRRGVPALHTKTGVLSAVHIGNYMSARVIELLSKYT 130 Q++ A E IH A+L+HDD+ID ++ RRG + K +A+ GN++ A +E L Sbjct: 66 QVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWDQTTAILTGNFLLALGLEHL---M 122 Query: 131 GDKNRYVHDLSSVATAQLCLGEYQQMEHAYDYNLTFDEYLEKSRNKTAMLMATCLRVGAL 190 K+ VH L S + +C GE Q + ++ T YL + KTA+L+ VGA+ Sbjct: 123 AVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIINYLRRINRKTALLIQISTEVGAI 182 Query: 191 SAESPEEVAGLLYDFGEALGMSFQIQDDLLDFTQSADVLGKPAGSDLRNGQVTLPVLFAL 250 +++S +E L G +GMSFQI DD+LDFT + LGKP GSDL NG +TLP+L + Sbjct: 183 TSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTEKKLGKPVGSDLLNGHITLPILLEM 242 Query: 251 -QDPELAPVIRTIGPASLEEEVAHVLALINRSDALFRTEEVSQDYLGQAAAIIQRLSSYP 309 ++P+ I + S +E + +I +SD++ ++ VS YL +A +I L Sbjct: 243 RKNPDFKLKIEQLRRDSDRKEFEECIQIIRKSDSINESKAVSSKYLSKALDLISELPDGH 302 Query: 310 AHADLETLLQYFAGRD 325 + L +L + R+ Sbjct: 303 PKSLLLSLTKKMGSRN 318 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.134 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 439,779,836 Number of extensions: 17226362 Number of successful extensions: 50714 Number of sequences better than 1.0e-05: 1422 Number of HSP's gapped: 48670 Number of HSP's successfully gapped: 1426 Length of query: 326 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 200 Effective length of database: 415,135,104 Effective search space: 83027020800 Effective search space used: 83027020800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)