BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0016.p (1683 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00060160.1| COG2273: Beta-glucanase/Beta-glucan synthetas... 427 e-117 emb|CAC27412.2| endo-1,3(4)-beta-glucanase [Clostridium thermoce... 427 e-117 pir||T18265 endo-1,3(4)-beta-glucanase (EC 3.2.1.6) - Clostridiu... 417 e-114 sp|P23903|E13B_BACCI GLUCAN ENDO-1,3-BETA-GLUCOSIDASE A1 PRECURS... 236 3e-60 ref|NP_227840.1| laminarinase [Thermotoga maritima] >gi|7462867|... 232 6e-59 >ref|ZP_00060160.1| COG2273: Beta-glucanase/Beta-glucan synthetase [Clostridium thermocellum ATCC 27405] Length = 1321 Score = 427 bits (1099), Expect = e-117, Method: Compositional matrix adjust. Identities = 277/779 (35%), Positives = 416/779 (53%), Gaps = 59/779 (7%) Query: 516 DPWTLVWNDEFNDGTIDPAKWTYDLGDGTAVGNPGWGNNELEWYTNEEKNVKEADGKLII 575 + W LVW+DEFN I+ A W+YD D T N W N E++ YT +K DG L+I Sbjct: 423 EEWRLVWSDEFNGSEINMANWSYD--DPT---NGRW-NGEVQSYTQNNAYIK--DGALVI 474 Query: 576 TARKE-----AQGGKEYTSSRIKTKDLFSKKYGKFEIRAKAPAGKGYWPAIWMLPEDY-V 629 ARKE + YTSS++ TK S KYGKFEIRAK P G+G WPAIWM+PED Sbjct: 475 EARKEDITEPSGETYHYTSSKLITKGKKSWKYGKFEIRAKMPQGQGIWPAIWMMPEDEPF 534 Query: 630 YGSWAASGEIDIMEGWGSRPGTVAGTIHYGSQWPDNVYSGKEYVLP-GNSTIEQFHTYSL 688 YG+W GEIDIME G P + GTIH+G P G Y LP G + + FH YS+ Sbjct: 535 YGTWPKCGEIDIMELLGHEPDKIYGTIHFGE--PHKESQGT-YTLPEGQTFADDFHVYSI 591 Query: 689 EWEPGELRWYVDGVLYSTKNDWYSLSNGQPANNAYPAPFNQEFHLLMNLAVGGNFDGNPT 748 EWEPGE+RWY+DG LY NDWYS + YPAPF+Q F L++N++VGG + G P Sbjct: 592 EWEPGEIRWYIDGKLYHVANDWYSRDPYLADDYTYPAPFDQNFFLILNISVGGGWPGYPD 651 Query: 749 AETVFPQSMEIDYVRVYELTGRPYRQPVPVTLTKEPYLEGSLP--PLADGNLIYNNGFTE 806 TVFPQ M +DYVRVY+ P+R+ +P E P PL DGN IYN GF Sbjct: 652 ETTVFPQQMVVDYVRVYQKDKYPHRE--------KPAKEEVKPREPLEDGNYIYNGGFDV 703 Query: 807 HKEGDTGM-GIPNTAHWVLYKESGADAAVSLEPISGRNYLKVNISSPGGNSYSIQ-PQAI 864 G+ G+P T++W SG A V++E + V I + G Y +Q QA Sbjct: 704 DDSAAVGVDGVPYTSYWTFLTASGGAATVNVE----EGVMHVQIENGGTTDYGVQLLQAP 759 Query: 865 VSLAKGRFYKLSFDAKTDHSRNIGVRLTGGESRGYQAYSPTLKAELTSTLKHYETMFQMK 924 + L KG YK SFD K ++ R + +++ G RG++ Y+ +++ + +YE F MK Sbjct: 760 IHLEKGAKYKASFDMKAENPRQVKLKIGGDGDRGWKDYAAIPPFTVSTEMTNYEFEFTMK 819 Query: 925 ENPDIAARVEFNLGTNASPVWLGNARLEEIDSIPFDHDS--AKTPLGSGNHLYNGTFDLG 982 ++ D+ AR EFN+G + + VW+ N +L + + P S A+ PL SGN++YNGTFD G Sbjct: 820 DDTDVKARFEFNMGLDDNDVWIDNVKLIKTEDAPVIDPSEIARPPLLSGNYIYNGTFDQG 879 Query: 983 EPDRLSYWH--AEATGGAAVSPSVDA-AGRLNLQISGTGGDGADVRLLQNGIFLVQGQDY 1039 P+R+ +W+ ++T A D R +I G +RL+Q GI + G+ Y Sbjct: 880 -PNRMGFWNFVVDSTAKATYYIGSDVNERRFETRIEKGGTSRGAIRLVQPGINIENGKTY 938 Query: 1040 KLAFDVEVPSPRTAIIELQG--RDGTVYASETVLLKAGAQKVEAVFPDLAGASDTLGRFV 1097 K++F+ + RT +E+ + +++A+ T + ++ E F + SD G Sbjct: 939 KVSFEASAANTRTIEVEIASNLHNSSIFAT-TFEISKESKIYEFEFT-MDKDSDKNGELR 996 Query: 1098 LHLGNAAGIVQLDNFLLLRTSF-YYDPSLVYYPLVNGDFSFGFNSWERLLTEQXXXXXXX 1156 +LG + V +DN ++ R S + +L+ + NG +G+ ++E + Sbjct: 997 FNLGGSNVNVYIDNVVMKRVSTDEVEGNLILNGVFNGLAGWGYGAYEPGSADFESHEEQF 1056 Query: 1157 XXXXXXXFNIANTGNQPYSVMLFQNGLKVAGGTDYVIEFDASSSVARRISVNAE-----N 1211 I++ GN+ ++V L+Q+ + + G Y + FDA S++ R+I V + + Sbjct: 1057 RAI------ISSVGNEGWNVQLYQDNVPLEQGQTYEVSFDAKSTIDRKIIVQLQRNGTSD 1110 Query: 1212 ASYQPSFTQIIKITPETNHYRFEF--RQGVNDTLALKFLLGKVDGVSIPQAHELVIDNV 1268 ++ F Q +++T E +++EF + + F LG + + HE++IDNV Sbjct: 1111 NNWDSYFYQEVELTNELKTFKYEFTMSKPTDSASRFNFALGNTENKTYA-PHEIIIDNV 1168 Score = 181 bits (460), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 98/293 (33%), Positives = 151/293 (51%), Gaps = 4/293 (1%) Query: 75 WAARAVQRWIENGIVAGYGDGSFRPDAPVSKAEFAVIINRIFNYSAKSATLPDDVPAAAW 134 WA + +W+E GI+ GY DG+ RPD +++AEF +IN++F S DV + W Sbjct: 37 WAEEDLNKWMEKGILVGYQDGTIRPDNNITRAEFVTLINKVFGLYELSREQFADVEDSKW 96 Query: 135 FSSEIAKGIAAGYLIASKDGRIEASAPLTRAETAVALQKIFRLDTGGQ--AAYSDLAGTD 192 +S EI K AAGY+ + +TR E V + K+F L +G + + D + Sbjct: 97 YSREILKARAAGYIAGYGSNVFKPDNYITRQEAVVIIAKVFELQSGSNYTSKFKDGSLVK 156 Query: 193 HEVRDAAAALSGAGYVKGYPDGSFKPQGRITRAELAQTLDRMVPGLVRSAGEVTLGKVQG 252 +D+ +AL GY+ GY DG+F+P ITRAE + L++++P L G+ +V G Sbjct: 157 EYAKDSVSALVEKGYIAGYEDGTFRPDNYITRAETIKILNKIIPSLYNEKGDYKNEEVAG 216 Query: 253 NVVLNHAGITLKDTVVEGNLYLSXXXXXXXXXXXXVKVTGTTFILGGGEQGVGLQNSVIG 312 N ++N G+ LKDTV+ G+LYL+ V V GT F+ GGG + N+ I Sbjct: 217 NALINTEGVILKDTVINGDLYLAQGIQNGDVTLDGVNVKGTVFVNGGGSDSIHFINTKIN 276 Query: 313 RVQAGKPDGDIRMYASGTTSVGTVLLNSXXXXXXXXXXXXXFTDVEISAAVKA 365 RV K +R+ SG TSV +V++ S F +V + + + A Sbjct: 277 RVVVNKT--GVRIVTSGNTSVESVVVKSGAKLEEKELTGDGFKNVTVDSQLSA 327 Score = 117 bits (294), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 88/284 (30%), Positives = 131/284 (46%), Gaps = 30/284 (10%) Query: 1405 IDVAGTAAWHTQFYQEGIPLEAGKTYELSFKAKSTVPRQIIVEY--SGTSAAAAQAKF-- 1460 I G W+ Q YQ+ +PLE G+TYE+SF AKST+ R+IIV+ +GTS + F Sbjct: 1060 ISSVGNEGWNVQLYQDNVPLEQGQTYEVSFDAKSTIDRKIIVQLQRNGTSDNNWDSYFYQ 1119 Query: 1461 --EVTATWATYSARFTVDNSNPLKLNYLIGAAMGEDKTANGIPHTLSFDDIRXXXXXXXX 1518 E+T T+ FT+ S P A+G + PH + D++ Sbjct: 1120 EVELTNELKTFKYEFTM--SKPTDSASRFNFALGNTENKTYAPHEIIIDNV--------V 1169 Query: 1519 XXXXXXASGKLDNGTFDPAKGLTGWTQYF-DGTGSAAAQDGELAVALTGTGTANYSAQVD 1577 S + NGTFD G+ W Y+ DG G+ DGEL + +T GTA+Y Q+ Sbjct: 1170 VRKVATPSALILNGTFD--DGMDHWLLYWGDGEGNCDVTDGELEINITKVGTADYMPQIK 1227 Query: 1578 YANLKLVQGKTYTLAFAARSDVDRLIEVAVEHKGGDYTKYLPAKAVALTGSMNTYSYTFT 1637 N+ L +G TYTL+ AR+ R I+V + + LT Y++TFT Sbjct: 1228 QENIALQEGVTYTLSLKARALEARSIKVDILDSS---YNWYGGTIFDLTTEDAVYTFTFT 1284 Query: 1638 MDSGTDAGAHLVFLLGLVAGNSEGTNSAIQAGNHIYIDNVSLTE 1681 + G L LG + G + A +Y+D++ L + Sbjct: 1285 QSKSINNGV-LTINLGTIEGKT-------SAATTVYLDDILLEQ 1320 Score = 104 bits (259), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 128/501 (25%), Positives = 212/501 (42%), Gaps = 56/501 (11%) Query: 787 EGSLPPLADGNLIYNNGFTEHKEGDTGMGIPNTAHWVLYKESGADAAVSLEPISGRNYLK 846 E + PPL GN IYN F +G MG W +S A A + + Sbjct: 859 EIARPPLLSGNYIYNGTF---DQGPNRMGF-----WNFVVDSTAKATYYIGSDVNERRFE 910 Query: 847 VNISSPGGNSYSIQ-PQAIVSLAKGRFYKLSFDAKTDHSRNIGVRLTGGESRGYQAYSPT 905 I G + +I+ Q +++ G+ YK+SF+A ++R I V + ++ T Sbjct: 911 TRIEKGGTSRGAIRLVQPGINIENGKTYKVSFEASAANTRTIEVEIASNLHNS-SIFATT 969 Query: 906 LKAELTSTLKHYETMFQMKENPDIAARVEFNLGTNASPVWLGNARLEEIDSIPFDHDSAK 965 E++ K YE F M ++ D + FNLG + V++ N ++ + Sbjct: 970 F--EISKESKIYEFEFTMDKDSDKNGELRFNLGGSNVNVYIDNVVMKRV----------S 1017 Query: 966 TPLGSGNHLYNGTFDLGEPDRLSYW-HAEATGGAAVSPSVDAAGRLNLQISGTGGDGADV 1024 T GN + NG F+ L+ W + G+A S + R IS G +G +V Sbjct: 1018 TDEVEGNLILNGVFN-----GLAGWGYGAYEPGSADFESHEEQFRA--IISSVGNEGWNV 1070 Query: 1025 RLLQNGIFLVQGQDYKLAFDVEVPSPRTAIIELQGRDGTV------YASETVLLKAGAQK 1078 +L Q+ + L QGQ Y+++FD + R I++LQ R+GT Y + V L + Sbjct: 1071 QLYQDNVPLEQGQTYEVSFDAKSTIDRKIIVQLQ-RNGTSDNNWDSYFYQEVELTNELKT 1129 Query: 1079 VEAVFPDLAGASDTLGRFVLHLGN------AAGIVQLDNFLLLRTSFYYDPSLVYYPLVN 1132 + F ++ +D+ RF LGN A + +DN ++ + + PS + ++N Sbjct: 1130 FKYEFT-MSKPTDSASRFNFALGNTENKTYAPHEIIIDNVVVRKVA---TPSAL---ILN 1182 Query: 1133 GDFSFGFNSWERLLTEQXXXXXXXXXXXXXXFNIANTGNQPYSVMLFQNGLKVAGGTDYV 1192 G F G + W LL NI G Y + Q + + G Y Sbjct: 1183 GTFDDGMDHW--LLYWGDGEGNCDVTDGELEINITKVGTADYMPQIKQENIALQEGVTYT 1240 Query: 1193 IEFDASSSVARRISVNAENASYQPSFTQIIKITPETNHYRFEFRQ--GVNDTLALKFLLG 1250 + A + AR I V+ ++SY I +T E Y F F Q +N+ + L LG Sbjct: 1241 LSLKARALEARSIKVDILDSSYNWYGGTIFDLTTEDAVYTFTFTQSKSINNGV-LTINLG 1299 Query: 1251 KVDGVSIPQAHELVIDNVRFE 1271 ++G + A + +D++ E Sbjct: 1300 TIEGKT-SAATTVYLDDILLE 1319 Score = 96.7 bits (239), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 80/292 (27%), Positives = 133/292 (45%), Gaps = 35/292 (11%) Query: 1395 NVNEGVAEIRIDVAGTAAWHTQFYQEGIPLEAGKTYELSFKAKSTVPRQIIVEYSGT--S 1452 +VNE E RI+ GT+ + Q GI +E GKTY++SF+A + R I VE + + Sbjct: 903 DVNERRFETRIEKGGTSRGAIRLVQPGINIENGKTYKVSFEASAANTRTIEVEIASNLHN 962 Query: 1453 AAAAQAKFEVTATWATYSARFTVDNSNPL--KLNYLIGAAMGEDKTANGIPHTLSFDDIR 1510 ++ FE++ Y FT+D + +L + +G + N + +S D++ Sbjct: 963 SSIFATTFEISKESKIYEFEFTMDKDSDKNGELRFNLGGSNVNVYIDNVVMKRVSTDEVE 1022 Query: 1511 XXXXXXXXXXXXXXASGKLDNGTFDPAKGLTGWT--QYFDGTGSAAAQDGELAVALTGTG 1568 + NG F+ GL GW Y G+ + + + ++ G Sbjct: 1023 ---------------GNLILNGVFN---GLAGWGYGAYEPGSADFESHEEQFRAIISSVG 1064 Query: 1569 TANYSAQVDYANLKLVQGKTYTLAFAARSDVDRLIEVAVEHKG---GDYTKYLPAKAVAL 1625 ++ Q+ N+ L QG+TY ++F A+S +DR I V ++ G ++ Y + V L Sbjct: 1065 NEGWNVQLYQDNVPLEQGQTYEVSFDAKSTIDRKIIVQLQRNGTSDNNWDSYF-YQEVEL 1123 Query: 1626 TGSMNTYSYTFTMDSGTDAGAHLVFLLGLVAGNSEGTNSAIQAGNHIYIDNV 1677 T + T+ Y FTM TD+ + F LG T + A + I IDNV Sbjct: 1124 TNELKTFKYEFTMSKPTDSASRFNFALG-------NTENKTYAPHEIIIDNV 1168 Score = 73.6 bits (179), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 76/298 (25%), Positives = 115/298 (38%), Gaps = 30/298 (10%) Query: 1395 NVNEGVAEIRIDVAGTAAWHTQFYQEGIPLEAGKTYELSFKAKSTVPRQIIVEYSGT--- 1451 NV EGV ++I+ GT + Q Q I LE G Y+ SF K+ PRQ+ ++ G Sbjct: 733 NVEEGVMHVQIENGGTTDYGVQLLQAPIHLEKGAKYKASFDMKAENPRQVKLKIGGDGDR 792 Query: 1452 --SAAAAQAKFEVTATWATYSARFTVDNSNPLKLNYLIGAAMGEDKTANGIPHTLSFDDI 1509 AA F V+ Y FT+ + +K + MG D I + Sbjct: 793 GWKDYAAIPPFTVSTEMTNYEFEFTMKDDTDVKARFEFN--MGLDDNDVWIDNVKLIKTE 850 Query: 1510 RXXXXXXXXXXXXXXASGK-LDNGTFDPAKGLTG-WTQYFDGTGSAA------AQDGELA 1561 SG + NGTFD G W D T A + Sbjct: 851 DAPVIDPSEIARPPLLSGNYIYNGTFDQGPNRMGFWNFVVDSTAKATYYIGSDVNERRFE 910 Query: 1562 VALTGTGTANYSAQVDYANLKLVQGKTYTLAFAARSDVDRLIEVAVEHKGGDYTKYLPAK 1621 + GT+ + ++ + + GKTY ++F A + R IEV + + + + A Sbjct: 911 TRIEKGGTSRGAIRLVQPGINIENGKTYKVSFEASAANTRTIEVEIASNLHNSSIF--AT 968 Query: 1622 AVALTGSMNTYSYTFTMDSGTDAGAHLVFLLGLVAGNSEGTNSAIQAGNHIYIDNVSL 1679 ++ Y + FTMD +D L F LG G+N ++YIDNV + Sbjct: 969 TFEISKESKIYEFEFTMDKDSDKNGELRFNLG-------GSNV------NVYIDNVVM 1013 >emb|CAC27412.2| endo-1,3(4)-beta-glucanase [Clostridium thermocellum] emb|CAA61884.2| endo-1,3(4)-beta-glucanase [Clostridium thermocellum] Length = 1321 Score = 427 bits (1097), Expect = e-117, Method: Compositional matrix adjust. Identities = 277/779 (35%), Positives = 415/779 (53%), Gaps = 59/779 (7%) Query: 516 DPWTLVWNDEFNDGTIDPAKWTYDLGDGTAVGNPGWGNNELEWYTNEEKNVKEADGKLII 575 + W LVW+DEFN I+ A W+YD D T N W N E++ YT +K DG L+I Sbjct: 423 EEWRLVWSDEFNGSEINMANWSYD--DPT---NGRW-NGEVQSYTQNNAYIK--DGALVI 474 Query: 576 TARKE-----AQGGKEYTSSRIKTKDLFSKKYGKFEIRAKAPAGKGYWPAIWMLPEDY-V 629 ARKE + YTSS++ TK S KYGKFEIRAK P G+G WPAIWM+PED Sbjct: 475 EARKEDITEPSGETYHYTSSKLITKGKKSWKYGKFEIRAKMPQGQGIWPAIWMMPEDEPF 534 Query: 630 YGSWAASGEIDIMEGWGSRPGTVAGTIHYGSQWPDNVYSGKEYVLP-GNSTIEQFHTYSL 688 YG+W GEIDIME G P + GTIH+G P G Y LP G + + FH YS+ Sbjct: 535 YGTWPKCGEIDIMELLGHEPDKIYGTIHFGE--PHKESQGT-YTLPEGQTFADDFHVYSI 591 Query: 689 EWEPGELRWYVDGVLYSTKNDWYSLSNGQPANNAYPAPFNQEFHLLMNLAVGGNFDGNPT 748 EWEPGE+RWY+DG LY NDWYS + YPAPF+Q F L++N++VGG + G P Sbjct: 592 EWEPGEIRWYIDGKLYHVANDWYSRDPYLADDYTYPAPFDQNFFLILNISVGGGWPGYPD 651 Query: 749 AETVFPQSMEIDYVRVYELTGRPYRQPVPVTLTKEPYLEGSLP--PLADGNLIYNNGFTE 806 TVFPQ M +DYVRVY+ P+R+ +P E P PL DGN IYN GF Sbjct: 652 ETTVFPQQMVVDYVRVYQKDKYPHRE--------KPAKEEVKPREPLEDGNYIYNGGFDV 703 Query: 807 HKEGDTGM-GIPNTAHWVLYKESGADAAVSLEPISGRNYLKVNISSPGGNSYSIQ-PQAI 864 G+ G+P T++W SG A V++E + V I + G Y +Q QA Sbjct: 704 DDSAAVGVDGVPYTSYWTFLTASGGAATVNVE----EGVMHVQIENGGTTDYGVQLLQAP 759 Query: 865 VSLAKGRFYKLSFDAKTDHSRNIGVRLTGGESRGYQAYSPTLKAELTSTLKHYETMFQMK 924 + L KG YK SFD K ++ R + +++ G RG++ Y+ +++ + +YE F MK Sbjct: 760 IHLEKGAKYKASFDMKAENPRQVKLKIGGDGDRGWKDYAAIPPFTVSTEMTNYEFEFTMK 819 Query: 925 ENPDIAARVEFNLGTNASPVWLGNARLEEIDSIPFDHDS--AKTPLGSGNHLYNGTFDLG 982 ++ D+ AR EFN+G + + VW+ N +L + + P S A+ PL SGN++YNGTFD G Sbjct: 820 DDTDVKARFEFNMGLDDNDVWIDNVKLIKTEDAPVIDPSEIARPPLLSGNYIYNGTFDQG 879 Query: 983 EPDRLSYWH--AEATGGAAVSPSVDA-AGRLNLQISGTGGDGADVRLLQNGIFLVQGQDY 1039 P+R+ +W+ ++T A D R +I G +RL+Q GI + G+ Y Sbjct: 880 -PNRMGFWNFVVDSTAKATYYIGSDVNERRFETRIEKGGTSRGAIRLVQPGINIENGKTY 938 Query: 1040 KLAFDVEVPSPRTAIIELQG--RDGTVYASETVLLKAGAQKVEAVFPDLAGASDTLGRFV 1097 K++F+ + RT +E+ + +++A+ T + ++ E F + SD G Sbjct: 939 KVSFEASAANTRTIEVEIASNLHNSSIFAT-TFEISKESKIYEFEFT-MDKDSDKNGELR 996 Query: 1098 LHLGNAAGIVQLDNFLLLRTSF-YYDPSLVYYPLVNGDFSFGFNSWERLLTEQXXXXXXX 1156 +LG + V +DN ++ R S + +L+ + NG +G+ +E + Sbjct: 997 FNLGGSNVNVYIDNVVMKRVSTDEVEGNLILNGVFNGLAGWGYGGYEPGSADFESHEEQF 1056 Query: 1157 XXXXXXXFNIANTGNQPYSVMLFQNGLKVAGGTDYVIEFDASSSVARRISVNAE-----N 1211 I++ GN+ ++V L+Q+ + + G Y + FDA S++ R+I V + + Sbjct: 1057 RAI------ISSVGNEGWNVQLYQDNVPLEQGQTYEVSFDAKSTIDRKIIVQLQRNGTSD 1110 Query: 1212 ASYQPSFTQIIKITPETNHYRFEF--RQGVNDTLALKFLLGKVDGVSIPQAHELVIDNV 1268 ++ F Q +++T E +++EF + + F LG + + HE++IDNV Sbjct: 1111 NNWDSYFYQEVELTNELKTFKYEFTMSKPTDSASRFNFALGNTENKTYA-PHEIIIDNV 1168 Score = 181 bits (460), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 98/293 (33%), Positives = 151/293 (51%), Gaps = 4/293 (1%) Query: 75 WAARAVQRWIENGIVAGYGDGSFRPDAPVSKAEFAVIINRIFNYSAKSATLPDDVPAAAW 134 WA + +W+E GI+ GY DG+ RPD +++AEF +IN++F S DV + W Sbjct: 37 WAEEDLNKWMEKGILVGYQDGTIRPDNNITRAEFVTLINKVFGLYELSREQFADVEDSKW 96 Query: 135 FSSEIAKGIAAGYLIASKDGRIEASAPLTRAETAVALQKIFRLDTGGQ--AAYSDLAGTD 192 +S EI K AAGY+ + +TR E V + K+F L +G + + D + Sbjct: 97 YSREILKARAAGYIAGYGSNVFKPDNYITRQEAVVIIAKVFELQSGSNYTSKFKDGSLVK 156 Query: 193 HEVRDAAAALSGAGYVKGYPDGSFKPQGRITRAELAQTLDRMVPGLVRSAGEVTLGKVQG 252 +D+ +AL GY+ GY DG+F+P ITRAE + L++++P L G+ +V G Sbjct: 157 EYAKDSVSALVEKGYIAGYEDGTFRPDNYITRAETIKILNKIIPSLYNEKGDYKNEEVAG 216 Query: 253 NVVLNHAGITLKDTVVEGNLYLSXXXXXXXXXXXXVKVTGTTFILGGGEQGVGLQNSVIG 312 N ++N G+ LKDTV+ G+LYL+ V V GT F+ GGG + N+ I Sbjct: 217 NALINTEGVILKDTVINGDLYLAQGIQNGDVTLDGVNVKGTVFVNGGGSDSIHFINTKIN 276 Query: 313 RVQAGKPDGDIRMYASGTTSVGTVLLNSXXXXXXXXXXXXXFTDVEISAAVKA 365 RV K +R+ SG TSV +V++ S F +V + + + A Sbjct: 277 RVVVNKT--GVRIVTSGNTSVESVVVKSGAKLEEKELTGDGFKNVTVDSQLSA 327 Score = 117 bits (294), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 88/284 (30%), Positives = 131/284 (46%), Gaps = 30/284 (10%) Query: 1405 IDVAGTAAWHTQFYQEGIPLEAGKTYELSFKAKSTVPRQIIVEY--SGTSAAAAQAKF-- 1460 I G W+ Q YQ+ +PLE G+TYE+SF AKST+ R+IIV+ +GTS + F Sbjct: 1060 ISSVGNEGWNVQLYQDNVPLEQGQTYEVSFDAKSTIDRKIIVQLQRNGTSDNNWDSYFYQ 1119 Query: 1461 --EVTATWATYSARFTVDNSNPLKLNYLIGAAMGEDKTANGIPHTLSFDDIRXXXXXXXX 1518 E+T T+ FT+ S P A+G + PH + D++ Sbjct: 1120 EVELTNELKTFKYEFTM--SKPTDSASRFNFALGNTENKTYAPHEIIIDNV--------V 1169 Query: 1519 XXXXXXASGKLDNGTFDPAKGLTGWTQYF-DGTGSAAAQDGELAVALTGTGTANYSAQVD 1577 S + NGTFD G+ W Y+ DG G+ DGEL + +T GTA+Y Q+ Sbjct: 1170 VRKVATPSALILNGTFD--DGMDHWLLYWGDGEGNCDVTDGELEINITKVGTADYMPQIK 1227 Query: 1578 YANLKLVQGKTYTLAFAARSDVDRLIEVAVEHKGGDYTKYLPAKAVALTGSMNTYSYTFT 1637 N+ L +G TYTL+ AR+ R I+V + + LT Y++TFT Sbjct: 1228 QENIALQEGVTYTLSLKARALEARSIKVDILDSS---YNWYGGTIFDLTTEDAVYTFTFT 1284 Query: 1638 MDSGTDAGAHLVFLLGLVAGNSEGTNSAIQAGNHIYIDNVSLTE 1681 + G L LG + G + A +Y+D++ L + Sbjct: 1285 QSKSINNGV-LTINLGTIEGKT-------SAATTVYLDDILLEQ 1320 Score = 103 bits (257), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 128/501 (25%), Positives = 212/501 (42%), Gaps = 56/501 (11%) Query: 787 EGSLPPLADGNLIYNNGFTEHKEGDTGMGIPNTAHWVLYKESGADAAVSLEPISGRNYLK 846 E + PPL GN IYN F +G MG W +S A A + + Sbjct: 859 EIARPPLLSGNYIYNGTF---DQGPNRMGF-----WNFVVDSTAKATYYIGSDVNERRFE 910 Query: 847 VNISSPGGNSYSIQ-PQAIVSLAKGRFYKLSFDAKTDHSRNIGVRLTGGESRGYQAYSPT 905 I G + +I+ Q +++ G+ YK+SF+A ++R I V + ++ T Sbjct: 911 TRIEKGGTSRGAIRLVQPGINIENGKTYKVSFEASAANTRTIEVEIASNLHNS-SIFATT 969 Query: 906 LKAELTSTLKHYETMFQMKENPDIAARVEFNLGTNASPVWLGNARLEEIDSIPFDHDSAK 965 E++ K YE F M ++ D + FNLG + V++ N ++ + Sbjct: 970 F--EISKESKIYEFEFTMDKDSDKNGELRFNLGGSNVNVYIDNVVMKRV----------S 1017 Query: 966 TPLGSGNHLYNGTFDLGEPDRLSYW-HAEATGGAAVSPSVDAAGRLNLQISGTGGDGADV 1024 T GN + NG F+ L+ W + G+A S + R IS G +G +V Sbjct: 1018 TDEVEGNLILNGVFN-----GLAGWGYGGYEPGSADFESHEEQFRA--IISSVGNEGWNV 1070 Query: 1025 RLLQNGIFLVQGQDYKLAFDVEVPSPRTAIIELQGRDGTV------YASETVLLKAGAQK 1078 +L Q+ + L QGQ Y+++FD + R I++LQ R+GT Y + V L + Sbjct: 1071 QLYQDNVPLEQGQTYEVSFDAKSTIDRKIIVQLQ-RNGTSDNNWDSYFYQEVELTNELKT 1129 Query: 1079 VEAVFPDLAGASDTLGRFVLHLGN------AAGIVQLDNFLLLRTSFYYDPSLVYYPLVN 1132 + F ++ +D+ RF LGN A + +DN ++ + + PS + ++N Sbjct: 1130 FKYEFT-MSKPTDSASRFNFALGNTENKTYAPHEIIIDNVVVRKVA---TPSAL---ILN 1182 Query: 1133 GDFSFGFNSWERLLTEQXXXXXXXXXXXXXXFNIANTGNQPYSVMLFQNGLKVAGGTDYV 1192 G F G + W LL NI G Y + Q + + G Y Sbjct: 1183 GTFDDGMDHW--LLYWGDGEGNCDVTDGELEINITKVGTADYMPQIKQENIALQEGVTYT 1240 Query: 1193 IEFDASSSVARRISVNAENASYQPSFTQIIKITPETNHYRFEFRQ--GVNDTLALKFLLG 1250 + A + AR I V+ ++SY I +T E Y F F Q +N+ + L LG Sbjct: 1241 LSLKARALEARSIKVDILDSSYNWYGGTIFDLTTEDAVYTFTFTQSKSINNGV-LTINLG 1299 Query: 1251 KVDGVSIPQAHELVIDNVRFE 1271 ++G + A + +D++ E Sbjct: 1300 TIEGKT-SAATTVYLDDILLE 1319 Score = 96.7 bits (239), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 80/292 (27%), Positives = 133/292 (45%), Gaps = 35/292 (11%) Query: 1395 NVNEGVAEIRIDVAGTAAWHTQFYQEGIPLEAGKTYELSFKAKSTVPRQIIVEYSGT--S 1452 +VNE E RI+ GT+ + Q GI +E GKTY++SF+A + R I VE + + Sbjct: 903 DVNERRFETRIEKGGTSRGAIRLVQPGINIENGKTYKVSFEASAANTRTIEVEIASNLHN 962 Query: 1453 AAAAQAKFEVTATWATYSARFTVDNSNPL--KLNYLIGAAMGEDKTANGIPHTLSFDDIR 1510 ++ FE++ Y FT+D + +L + +G + N + +S D++ Sbjct: 963 SSIFATTFEISKESKIYEFEFTMDKDSDKNGELRFNLGGSNVNVYIDNVVMKRVSTDEVE 1022 Query: 1511 XXXXXXXXXXXXXXASGKLDNGTFDPAKGLTGWTQ--YFDGTGSAAAQDGELAVALTGTG 1568 + NG F+ GL GW Y G+ + + + ++ G Sbjct: 1023 ---------------GNLILNGVFN---GLAGWGYGGYEPGSADFESHEEQFRAIISSVG 1064 Query: 1569 TANYSAQVDYANLKLVQGKTYTLAFAARSDVDRLIEVAVEHKG---GDYTKYLPAKAVAL 1625 ++ Q+ N+ L QG+TY ++F A+S +DR I V ++ G ++ Y + V L Sbjct: 1065 NEGWNVQLYQDNVPLEQGQTYEVSFDAKSTIDRKIIVQLQRNGTSDNNWDSYF-YQEVEL 1123 Query: 1626 TGSMNTYSYTFTMDSGTDAGAHLVFLLGLVAGNSEGTNSAIQAGNHIYIDNV 1677 T + T+ Y FTM TD+ + F LG T + A + I IDNV Sbjct: 1124 TNELKTFKYEFTMSKPTDSASRFNFALG-------NTENKTYAPHEIIIDNV 1168 Score = 73.6 bits (179), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 76/298 (25%), Positives = 115/298 (38%), Gaps = 30/298 (10%) Query: 1395 NVNEGVAEIRIDVAGTAAWHTQFYQEGIPLEAGKTYELSFKAKSTVPRQIIVEYSGT--- 1451 NV EGV ++I+ GT + Q Q I LE G Y+ SF K+ PRQ+ ++ G Sbjct: 733 NVEEGVMHVQIENGGTTDYGVQLLQAPIHLEKGAKYKASFDMKAENPRQVKLKIGGDGDR 792 Query: 1452 --SAAAAQAKFEVTATWATYSARFTVDNSNPLKLNYLIGAAMGEDKTANGIPHTLSFDDI 1509 AA F V+ Y FT+ + +K + MG D I + Sbjct: 793 GWKDYAAIPPFTVSTEMTNYEFEFTMKDDTDVKARFEFN--MGLDDNDVWIDNVKLIKTE 850 Query: 1510 RXXXXXXXXXXXXXXASGK-LDNGTFDPAKGLTG-WTQYFDGTGSAA------AQDGELA 1561 SG + NGTFD G W D T A + Sbjct: 851 DAPVIDPSEIARPPLLSGNYIYNGTFDQGPNRMGFWNFVVDSTAKATYYIGSDVNERRFE 910 Query: 1562 VALTGTGTANYSAQVDYANLKLVQGKTYTLAFAARSDVDRLIEVAVEHKGGDYTKYLPAK 1621 + GT+ + ++ + + GKTY ++F A + R IEV + + + + A Sbjct: 911 TRIEKGGTSRGAIRLVQPGINIENGKTYKVSFEASAANTRTIEVEIASNLHNSSIF--AT 968 Query: 1622 AVALTGSMNTYSYTFTMDSGTDAGAHLVFLLGLVAGNSEGTNSAIQAGNHIYIDNVSL 1679 ++ Y + FTMD +D L F LG G+N ++YIDNV + Sbjct: 969 TFEISKESKIYEFEFTMDKDSDKNGELRFNLG-------GSNV------NVYIDNVVM 1013 >pir||T18265 endo-1,3(4)-beta-glucanase (EC 3.2.1.6) - Clostridium thermocellum Length = 1324 Score = 417 bits (1071), Expect = e-114, Method: Compositional matrix adjust. Identities = 273/779 (35%), Positives = 411/779 (52%), Gaps = 58/779 (7%) Query: 516 DPWTLVWNDEFNDGTIDPAKWTYDLGDGTAVGNPGWGNNELEWYTNEEKNVKEADGKLII 575 + W LVW+DEFN I+ A W+YD D T N W N E++ YT +K DG L+I Sbjct: 425 EEWRLVWSDEFNGSEINMANWSYD--DPT---NGRW-NGEVQSYTQNNAYIK--DGALVI 476 Query: 576 TARKE-----AQGGKEYTSSRIKTKDLFSKKYGKFEIRAKAPAGKGYWPAIWMLPEDY-V 629 ARKE + YTSS++ TK S KYGKFEIRAK P G+G WPAIWM+PED Sbjct: 477 EARKEDITEPSGETYHYTSSKLITKGKKSWKYGKFEIRAKMPQGQGIWPAIWMMPEDEPF 536 Query: 630 YGSWAASGEIDIMEGWGSRPGTVAGTIHYGSQWPDNVYSGKEYVLP-GNSTIEQFHTYSL 688 YG+W GEIDIME G P + GTIH+G P G Y LP G + + FH YS+ Sbjct: 537 YGTWPKCGEIDIMELLGHEPDKIYGTIHFGE--PHKESQGT-YTLPEGQTFADDFHVYSI 593 Query: 689 EWEPGELRWYVDGVLYSTKNDWYSLSNGQPANNAYPAPFNQEFHLLMNLAVGGNFDGNPT 748 EWEPGE+RWY+DG LY NDWYS + YPAPF+Q F L++N++VGG + G P Sbjct: 594 EWEPGEIRWYIDGKLYHVANDWYSRDPYLADDYTYPAPFDQNFFLILNISVGGGWPGYPD 653 Query: 749 AETVFPQSMEIDYVRVYELTGRPYRQPVPVTLTKEPYLEGSLP--PLADGNLIYNNGFTE 806 TVFPQ M +DYVRVY+ P+R+ +P E P PL DGN IYN GF Sbjct: 654 ETTVFPQQMVVDYVRVYQKDKYPHRE--------KPAKEEVKPREPLEDGNYIYNGGFDV 705 Query: 807 HKEGDTGM-GIPNTAHWVLYKESGADAAVSLEPISGRNYLKVNISSPGGNSYSIQ-PQAI 864 G+ G+P T++W SG A V++E + V I + G Y +Q QA Sbjct: 706 DDSAAVGVDGVPYTSYWTFLTASGGAATVNVE----EGVMHVQIENGGTTDYGVQLLQAP 761 Query: 865 VSLAKGRFYKLSFDAKTDHSRNIGVRLTGGESRGYQAYSPTLKAELTSTLKHYETMFQMK 924 + L KG YK SFD K ++ R + +++ G RG++ Y+ +++ + +YE F MK Sbjct: 762 IHLEKGAKYKASFDMKAENPRQVKLKIGGDGDRGWKDYAAIPPFTVSTEMTNYEFEFTMK 821 Query: 925 ENPDIAARVEFNLGTNASPVWLGNARLEEIDSIPFDHDS--AKTPLGSGNHLYNGTFDLG 982 ++ D+ AR EFN+G + + VW+ N +L + + P S A+ PL SGN++YNGT Sbjct: 822 DDTDVKARFEFNMGLDDNDVWIDNVKLIKTEDAPVIDPSEIARPPLLSGNYIYNGTLLPR 881 Query: 983 EPDRLSYWH--AEATGGAAVSPSVDA-AGRLNLQISGTGGDGADVRLLQNGIFLVQGQDY 1039 +R+ +W+ ++T A D R +I G +RL+Q GI + G+ Y Sbjct: 882 SGNRMGFWNFVVDSTAKATYYIGSDVNERRFETRIEKGGTSRGAIRLVQPGINIENGKTY 941 Query: 1040 KLAFDVEVPSPRTAIIELQG--RDGTVYASETVLLKAGAQKVEAVFPDLAGASDTLGRFV 1097 K++F+ + RT +E+ + +++A+ T + ++ E F + SD G Sbjct: 942 KVSFEASAANTRTIEVEIASNLHNSSIFAT-TFEISKESKIYEFEFT-MDKDSDKNGELR 999 Query: 1098 LHLGNAAGIVQLDNFLLLRTSF-YYDPSLVYYPLVNGDFSFGFNSWERLLTEQXXXXXXX 1156 +LG + V +DN ++ R S + +L+ + NG +G+ +E + Sbjct: 1000 FNLGGSNVNVYIDNVVMKRVSTDEVEGNLILNGVFNGLAGWGYGGYEPGSADFESHEEQF 1059 Query: 1157 XXXXXXXFNIANTGNQPYSVMLFQNGLKVAGGTDYVIEFDASSSVARRISVNAE-----N 1211 I++ GN+ ++V L+Q+ + + G Y + FDA S++ R+I V + + Sbjct: 1060 RAI------ISSVGNEGWNVQLYQDNVPLEQGQTYEVSFDAKSTIDRKIIVQLQRNGTSD 1113 Query: 1212 ASYQPSFTQIIKITPETNHYRFEF--RQGVNDTLALKFLLGKVDGVSIPQAHELVIDNV 1268 ++ F Q +++T E +++EF + + F LG + + HE++IDNV Sbjct: 1114 NNWDSYFYQEVELTNELKTFKYEFTMSKPTDSASRFNFALGNTENKTYA-PHEIIIDNV 1171 Score = 171 bits (434), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 97/295 (32%), Positives = 151/295 (51%), Gaps = 6/295 (2%) Query: 75 WAARAVQRWIENGIVAGYGDGSFRPDAPVSKAEFAVIINRIFNYSAKSATLPDDVPAAAW 134 WA + +W+E GI+ GY DG+ RPD +++AEF +IN++F S DV + W Sbjct: 37 WAEEDLNKWMEKGILVGYQDGTIRPDNNITRAEFVTLINKVFGLYELSREQFADVEDSKW 96 Query: 135 FSSEIAKGIAAGYLIASKDGRIEASAPLTRAETAVALQKIFRLDTGGQ--AAYSDLAGTD 192 +S EI K AAGY+ + +TR E V + K+F L +G + + D + Sbjct: 97 YSREILKARAAGYIAGYGSNVFKPDNYITRQEAVVIIAKVFELQSGSNYTSKFKDGSLVK 156 Query: 193 HEVRDAAAALSGAGYVKGYPDGSFKPQGRITRAELAQTLDRMVPGLVRSAGEVTLGKVQG 252 +D+ +AL GY+ GY DG+F+P ITRAE + L++++P L G+ +V G Sbjct: 157 EYAKDSVSALVEKGYIAGYEDGTFRPDNYITRAETIKILNKIIPSLYNEKGDYKNEEVAG 216 Query: 253 NVVLNHAGITLKDTVVEGNLYLSXXXXXXXXXXXXVKVTGTTFILGGG--EQGVGLQNSV 310 N ++N G+ LKDTV+ G+LYL+ V V GT F+ GGG + ++ Sbjct: 217 NALINTEGVILKDTVINGDLYLAQGIQNGDVTLDGVNVKGTVFVNGGGSDQHYHFIKLPK 276 Query: 311 IGRVQAGKPDGDIRMYASGTTSVGTVLLNSXXXXXXXXXXXXXFTDVEISAAVKA 365 I RV K +R+ SG TSV +V++ S F +V + + + A Sbjct: 277 INRVVVNKT--GVRIVTSGNTSVESVVVKSGAKLEEKELTGDGFKNVTVDSQLSA 329 Score = 117 bits (294), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 88/284 (30%), Positives = 131/284 (46%), Gaps = 30/284 (10%) Query: 1405 IDVAGTAAWHTQFYQEGIPLEAGKTYELSFKAKSTVPRQIIVEY--SGTSAAAAQAKF-- 1460 I G W+ Q YQ+ +PLE G+TYE+SF AKST+ R+IIV+ +GTS + F Sbjct: 1063 ISSVGNEGWNVQLYQDNVPLEQGQTYEVSFDAKSTIDRKIIVQLQRNGTSDNNWDSYFYQ 1122 Query: 1461 --EVTATWATYSARFTVDNSNPLKLNYLIGAAMGEDKTANGIPHTLSFDDIRXXXXXXXX 1518 E+T T+ FT+ S P A+G + PH + D++ Sbjct: 1123 EVELTNELKTFKYEFTM--SKPTDSASRFNFALGNTENKTYAPHEIIIDNV--------V 1172 Query: 1519 XXXXXXASGKLDNGTFDPAKGLTGWTQYF-DGTGSAAAQDGELAVALTGTGTANYSAQVD 1577 S + NGTFD G+ W Y+ DG G+ DGEL + +T GTA+Y Q+ Sbjct: 1173 VRKVATPSALILNGTFD--DGMDHWLLYWGDGEGNCDVTDGELEINITKVGTADYMPQIK 1230 Query: 1578 YANLKLVQGKTYTLAFAARSDVDRLIEVAVEHKGGDYTKYLPAKAVALTGSMNTYSYTFT 1637 N+ L +G TYTL+ AR+ R I+V + + LT Y++TFT Sbjct: 1231 QENIALQEGVTYTLSLKARALEARSIKVDILDSS---YNWYGGTIFDLTTEDAVYTFTFT 1287 Query: 1638 MDSGTDAGAHLVFLLGLVAGNSEGTNSAIQAGNHIYIDNVSLTE 1681 + G L LG + G + A +Y+D++ L + Sbjct: 1288 QSKSINNGV-LTINLGTIEGKT-------SAATTVYLDDILLEQ 1323 Score = 99.4 bits (246), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 125/501 (24%), Positives = 208/501 (41%), Gaps = 55/501 (10%) Query: 787 EGSLPPLADGNLIYNNGFTEHKEGDTGMGIPNTAHWVLYKESGADAAVSLEPISGRNYLK 846 E + PPL GN IYN G W +S A A + + Sbjct: 861 EIARPPLLSGNYIYNGTLLPRSGNRMGF-------WNFVVDSTAKATYYIGSDVNERRFE 913 Query: 847 VNISSPGGNSYSIQ-PQAIVSLAKGRFYKLSFDAKTDHSRNIGVRLTGGESRGYQAYSPT 905 I G + +I+ Q +++ G+ YK+SF+A ++R I V + ++ T Sbjct: 914 TRIEKGGTSRGAIRLVQPGINIENGKTYKVSFEASAANTRTIEVEIASNLHNS-SIFATT 972 Query: 906 LKAELTSTLKHYETMFQMKENPDIAARVEFNLGTNASPVWLGNARLEEIDSIPFDHDSAK 965 E++ K YE F M ++ D + FNLG + V++ N ++ + Sbjct: 973 F--EISKESKIYEFEFTMDKDSDKNGELRFNLGGSNVNVYIDNVVMKRV----------S 1020 Query: 966 TPLGSGNHLYNGTFDLGEPDRLSYW-HAEATGGAAVSPSVDAAGRLNLQISGTGGDGADV 1024 T GN + NG F+ L+ W + G+A S + R IS G +G +V Sbjct: 1021 TDEVEGNLILNGVFN-----GLAGWGYGGYEPGSADFESHEEQFRA--IISSVGNEGWNV 1073 Query: 1025 RLLQNGIFLVQGQDYKLAFDVEVPSPRTAIIELQGRDGTV------YASETVLLKAGAQK 1078 +L Q+ + L QGQ Y+++FD + R I++LQ R+GT Y + V L + Sbjct: 1074 QLYQDNVPLEQGQTYEVSFDAKSTIDRKIIVQLQ-RNGTSDNNWDSYFYQEVELTNELKT 1132 Query: 1079 VEAVFPDLAGASDTLGRFVLHLGN------AAGIVQLDNFLLLRTSFYYDPSLVYYPLVN 1132 + F ++ +D+ RF LGN A + +DN ++ + + PS + ++N Sbjct: 1133 FKYEFT-MSKPTDSASRFNFALGNTENKTYAPHEIIIDNVVVRKVA---TPSAL---ILN 1185 Query: 1133 GDFSFGFNSWERLLTEQXXXXXXXXXXXXXXFNIANTGNQPYSVMLFQNGLKVAGGTDYV 1192 G F G + W LL NI G Y + Q + + G Y Sbjct: 1186 GTFDDGMDHW--LLYWGDGEGNCDVTDGELEINITKVGTADYMPQIKQENIALQEGVTYT 1243 Query: 1193 IEFDASSSVARRISVNAENASYQPSFTQIIKITPETNHYRFEFRQ--GVNDTLALKFLLG 1250 + A + AR I V+ ++SY I +T E Y F F Q +N+ + L LG Sbjct: 1244 LSLKARALEARSIKVDILDSSYNWYGGTIFDLTTEDAVYTFTFTQSKSINNGV-LTINLG 1302 Query: 1251 KVDGVSIPQAHELVIDNVRFE 1271 ++G + A + +D++ E Sbjct: 1303 TIEGKT-SAATTVYLDDILLE 1322 Score = 96.7 bits (239), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 80/292 (27%), Positives = 133/292 (45%), Gaps = 35/292 (11%) Query: 1395 NVNEGVAEIRIDVAGTAAWHTQFYQEGIPLEAGKTYELSFKAKSTVPRQIIVEYSGT--S 1452 +VNE E RI+ GT+ + Q GI +E GKTY++SF+A + R I VE + + Sbjct: 906 DVNERRFETRIEKGGTSRGAIRLVQPGINIENGKTYKVSFEASAANTRTIEVEIASNLHN 965 Query: 1453 AAAAQAKFEVTATWATYSARFTVDNSNPL--KLNYLIGAAMGEDKTANGIPHTLSFDDIR 1510 ++ FE++ Y FT+D + +L + +G + N + +S D++ Sbjct: 966 SSIFATTFEISKESKIYEFEFTMDKDSDKNGELRFNLGGSNVNVYIDNVVMKRVSTDEVE 1025 Query: 1511 XXXXXXXXXXXXXXASGKLDNGTFDPAKGLTGWTQ--YFDGTGSAAAQDGELAVALTGTG 1568 + NG F+ GL GW Y G+ + + + ++ G Sbjct: 1026 ---------------GNLILNGVFN---GLAGWGYGGYEPGSADFESHEEQFRAIISSVG 1067 Query: 1569 TANYSAQVDYANLKLVQGKTYTLAFAARSDVDRLIEVAVEHKG---GDYTKYLPAKAVAL 1625 ++ Q+ N+ L QG+TY ++F A+S +DR I V ++ G ++ Y + V L Sbjct: 1068 NEGWNVQLYQDNVPLEQGQTYEVSFDAKSTIDRKIIVQLQRNGTSDNNWDSYF-YQEVEL 1126 Query: 1626 TGSMNTYSYTFTMDSGTDAGAHLVFLLGLVAGNSEGTNSAIQAGNHIYIDNV 1677 T + T+ Y FTM TD+ + F LG T + A + I IDNV Sbjct: 1127 TNELKTFKYEFTMSKPTDSASRFNFALG-------NTENKTYAPHEIIIDNV 1171 Score = 70.5 bits (171), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 75/299 (25%), Positives = 115/299 (38%), Gaps = 31/299 (10%) Query: 1395 NVNEGVAEIRIDVAGTAAWHTQFYQEGIPLEAGKTYELSFKAKSTVPRQIIVEYSGT--- 1451 NV EGV ++I+ GT + Q Q I LE G Y+ SF K+ PRQ+ ++ G Sbjct: 735 NVEEGVMHVQIENGGTTDYGVQLLQAPIHLEKGAKYKASFDMKAENPRQVKLKIGGDGDR 794 Query: 1452 --SAAAAQAKFEVTATWATYSARFTVDNSNPLKLNYLIGAAMGEDKTANGIPHTLSFDDI 1509 AA F V+ Y FT+ + +K + MG D I + Sbjct: 795 GWKDYAAIPPFTVSTEMTNYEFEFTMKDDTDVKARFEFN--MGLDDNDVWIDNVKLIKTE 852 Query: 1510 RXXXXXXXXXXXXXXASGK-LDNGTFDPAKG--LTGWTQYFDGTGSAA------AQDGEL 1560 SG + NGT P G + W D T A + Sbjct: 853 DAPVIDPSEIARPPLLSGNYIYNGTLLPRSGNRMGFWNFVVDSTAKATYYIGSDVNERRF 912 Query: 1561 AVALTGTGTANYSAQVDYANLKLVQGKTYTLAFAARSDVDRLIEVAVEHKGGDYTKYLPA 1620 + GT+ + ++ + + GKTY ++F A + R IEV + + + + A Sbjct: 913 ETRIEKGGTSRGAIRLVQPGINIENGKTYKVSFEASAANTRTIEVEIASNLHNSSIF--A 970 Query: 1621 KAVALTGSMNTYSYTFTMDSGTDAGAHLVFLLGLVAGNSEGTNSAIQAGNHIYIDNVSL 1679 ++ Y + FTMD +D L F LG G+N ++YIDNV + Sbjct: 971 TTFEISKESKIYEFEFTMDKDSDKNGELRFNLG-------GSNV------NVYIDNVVM 1016 Score = 57.4 bits (137), Expect = 3e-06, Method: Compositional matrix adjust. Identities = 34/117 (29%), Positives = 54/117 (46%), Gaps = 9/117 (7%) Query: 65 KAFTDVPADSWAARAVQRWIENGIVAGYGDGSFRPDAPVSKAEFAVIINRIF------NY 118 + F DV W +R + + G +AGYG F+PD +++ E VII ++F NY Sbjct: 86 EQFADVEDSKWYSREILKARAAGYIAGYGSNVFKPDNYITRQEAVVIIAKVFELQSGSNY 145 Query: 119 SAKSATLPDDVPAAAWFSSEIAKGIAAGYLIASKDGRIEASAPLTRAETAVALQKIF 175 ++K D + ++ + GY+ +DG +TRAET L KI Sbjct: 146 TSK---FKDGSLVKEYAKDSVSALVEKGYIAGYEDGTFRPDNYITRAETIKILNKII 199 >sp|P23903|E13B_BACCI GLUCAN ENDO-1,3-BETA-GLUCOSIDASE A1 PRECURSOR ((1->3)-BETA-GLUCAN ENDOHYDROLASE) ((1->3)-BETA-GLUCANASE A1) pir||JQ0420 beta-1,3-glucanase A1 precursor - Bacillus circulans gb|AAA22474.1| beta-1,3-glucanase A1 (glcA) Length = 682 Score = 236 bits (602), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 126/261 (48%), Positives = 164/261 (62%), Gaps = 19/261 (7%) Query: 520 LVWNDEFNDGTIDPAKWTYDLGD--GTAVGNPGWGNNELEWYTNEEKNVKEADGKLIITA 577 L+W DEFN T+D +KW Y+ G GWGN EL+ YTN +NV DGKL I A Sbjct: 425 LIWQDEFNGTTLDTSKWNYETGYYLNNDPATWGWGNAELQHYTNSTQNVYVQDGKLNIKA 484 Query: 578 RKEAQGGKE-------YTSSRIKTKDLFSKKYGKFEIRAKAPAGKGYWPAIWMLPEDYVY 630 +++ + Y+S +I TKD S KYG+ + RAK P G G WPA+WMLP+D VY Sbjct: 485 MNDSKSFPQDPNRYAQYSSGKINTKDKLSLKYGRVDFRAKLPTGDGVWPALWMLPKDSVY 544 Query: 631 GSWAASGEIDIMEGWGSRPGTVAGTIHYGSQWPDNVYSGKEYVLP-GNSTIEQFHTYSLE 689 G+WAASGEID+ME G PG+V+GTIH+G QWP N SG +Y P G + +H YS+ Sbjct: 545 GTWAASGEIDVMEARGRLPGSVSGTIHFGGQWPVNQSSGGDYHFPEGQTFANDYHVYSVV 604 Query: 690 WEPGELRWYVDGVLYS--TKNDWYSLSNGQPANNAYPAPFNQEFHLLMNLAVGGNFDG-- 745 WE ++WYVDG + T WYS + A N APF++ F+L+MNLAVGGNFDG Sbjct: 605 WEEDNIKWYVDGKFFYKVTNQQWYSTA----APNNPNAPFDEPFYLIMNLAVGGNFDGGR 660 Query: 746 NPTAETVFPQSMEIDYVRVYE 766 P A + P +M++DYVRVY+ Sbjct: 661 TPNASDI-PATMQVDYVRVYK 680 >ref|NP_227840.1| laminarinase [Thermotoga maritima] pir||B72428 laminarinase - Thermotoga maritima (strain MSB8) gb|AAD35118.1| laminarinase [Thermotoga maritima] Length = 642 Score = 232 bits (591), Expect = 6e-59, Method: Compositional matrix adjust. Identities = 157/437 (35%), Positives = 217/437 (49%), Gaps = 39/437 (8%) Query: 518 WTLVWNDEFNDGTIDPAKWTYDLGDGTAVGNPGWGNNELEWYTNEEKNVKEADGKLIITA 577 W LVW+ EF+DG IDP W +++G+G A G PGWGN ELE+YT+E V+ +G L+I A Sbjct: 212 WQLVWSQEFDDGVIDPNIWNFEIGNGHAKGIPGWGNGELEYYTDENAFVE--NGCLVIEA 269 Query: 578 RKEA----QGGKEYTSSRIKTKDLFSKKYGKFEIRAKAPAGKGYWPAIWMLPEDYVYGSW 633 RKE G +YTS+R+ T+ F KYGK EIRAK P GKG WPA+WML + W Sbjct: 270 RKEQVSDEYGTYDYTSARMTTEGKFEIKYGKIEIRAKLPKGKGIWPALWMLGNNIGEVGW 329 Query: 634 AASGEIDIMEGWGSRPGTVAGTIHYGSQWPDNVYSGKEYVLPGN--STIEQFHTYSLEWE 691 GEIDIME G TV GT H G + G Y LP E FH +S+EW+ Sbjct: 330 PTCGEIDIMEMLGHDTRTVYGTAH-GPGYSGGASIGVAYHLPEGVPDFSEDFHIFSIEWD 388 Query: 692 PGELRWYVDGVLYSTKNDWYSLSNGQPANNAYPAPFNQEFHLLMNLAVGGNFDGNPTAET 751 E+ WYVDG LY + LS + A F+ F L++N+AVGG + G P T Sbjct: 389 EDEVEWYVDGQLY------HVLSKDELAELGLEWVFDHPFFLILNVAVGGYWPGYPDETT 442 Query: 752 VFPQSMEIDYVRVYELTGRPYRQPVPVTLTKE------PYLEGSLPPLADGNLIYNNGFT 805 FPQ M IDY+RV Y+ P T+T E + P I N F Sbjct: 443 QFPQRMYIDYIRV-------YKDMNPETITGEVDDCEYEQAQQQAGPEVTYEQINNGTFD 495 Query: 806 EHKEGDTGMGIPNTAHWVLYKESGADAAVSLEPISG----RNYLKVNISSPGGNSYSIQP 861 E D N W +++ D +S +S Y + I+ PG +++ IQ Sbjct: 496 EPIVNDQAN---NPDEWFIWQ--AGDYGISGARVSDYGVRDGYAYITIADPGTDTWHIQF 550 Query: 862 QAIVSLAKGRFYKLSFDAKTDHSRNIGVRLTGGESRGYQAYSPTLKAELTSTLKHYETMF 921 + L +G+ Y +SF AK D R I V++ ++ T+ LT+ + + + Sbjct: 551 NQWIGLYRGKTYTISFKAKADTPRPINVKILQNHDPWTNYFAQTVN--LTADWQTFTFTY 608 Query: 922 QMKENPDIAARVEFNLG 938 ++ D ++ F LG Sbjct: 609 THPDDADEVVQISFELG 625 Score = 75.9 bits (185), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 52/171 (30%), Positives = 82/171 (47%), Gaps = 29/171 (16%) Query: 791 PPLADGNLIYNNGFTEHKEGDTGMGIPNTAHWVLYKESGADAAVSLEPISGRNYLKVNIS 850 PP DG++ DTG +WV + S + +E L V I+ Sbjct: 40 PPAEDGSI------------DTG------GNWVFFTNSNGEGTARVE----NGVLVVEIT 77 Query: 851 SPGGNSYSIQP-QAIVSLAKGRFYKLSFDAKTDHSRNIGVRLTGGESRGYQAYSP----- 904 + G +++S+Q QA + + K Y++SF AK +NIGV++ G RG+ AY+P Sbjct: 78 NGGDHTWSVQIIQAPIRVEKLHKYRVSFRAKASSQKNIGVKIGGTAGRGWTAYNPGTDES 137 Query: 905 -TLKAELTSTLKHYETMFQMKENPDIAARVEFNLGTNASPVWLGNARLEEI 954 + EL + + YE F M++ D AR EF LG VW+ + +E+I Sbjct: 138 GGMVFELGTDWQKYEFEFVMRQETDENARFEFQLGRYTGTVWIDDVVMEDI 188 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.314 0.132 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 2,341,516,269 Number of extensions: 100542210 Number of successful extensions: 172185 Number of sequences better than 1.0e-05: 321 Number of HSP's gapped: 171286 Number of HSP's successfully gapped: 356 Length of query: 1683 Length of database: 757,237,578 Length adjustment: 140 Effective length of query: 1543 Effective length of database: 377,123,718 Effective search space: 581901896874 Effective search space used: 581901896874 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)