BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0022.p (458 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_244930.1| thiophen and furan oxidation [Bacillus halodura... 561 e-158 ref|NP_694413.1| tRNA modification GTPase [Oceanobacillus iheyen... 553 e-156 ref|NP_765972.1| putative thiophene and furan oxidation protein ... 545 e-153 ref|NP_653951.1| MMR_HSR1, GTPase of unknown function [Bacillus ... 540 e-152 ref|NP_981927.1| tRNA modification GTPase TrmE [Bacillus cereus ... 539 e-152 >ref|NP_244930.1| thiophen and furan oxidation [Bacillus halodurans] sp|Q9RCA7|TRME_BACHD Probable tRNA modification GTPase trmE pir||F84157 thiophen and furan oxidation thdF [imported] - Bacillus halodurans (strain C-125) dbj|BAA82680.1| 77%-identity [Bacillus halodurans] dbj|BAB07781.1| thiophen and furan oxidation [Bacillus halodurans] Length = 458 Score = 561 bits (1446), Expect = e-158, Method: Compositional matrix adjust. Identities = 268/458 (58%), Positives = 361/458 (78%) Query: 1 MHSDTIAAVSTALGEGGIAIIRVSGPQAIAEVAPLFRSKTPLTEVESHTVHYGHIISPGD 60 M DTIAA+STALGEG I I+R+SG +A+ ++R K PL EV SHT+ YGHI+ P Sbjct: 1 MEFDTIAAISTALGEGAIGIVRLSGDEAVQLADRVYRGKKPLQEVPSHTIVYGHIVDPKT 60 Query: 61 GERMEEVLVTVMKGPRSFTTEDVVEISAHGGVISVRRVMDLLLQRDIRLAEPGEFTKRAF 120 E ++EV+V+V++ PR+FT EDV+EI+ HGG++SV RV+ L+L RLAEPGEFTKRAF Sbjct: 61 DEIIDEVMVSVLRAPRTFTREDVIEINCHGGLVSVNRVLQLVLNHGARLAEPGEFTKRAF 120 Query: 121 LGGRIDLSQAEAVIDLIRSKSDRAFSVALKQVSGSLSDKIHKLRHTLIEMLAHIEVNIDY 180 L GRIDLSQAE VIDLIR+K+DRA +VALKQV G LS KI LR L+E +AH+EVNIDY Sbjct: 121 LNGRIDLSQAEGVIDLIRAKTDRAMNVALKQVEGRLSTKISSLRQALLETVAHVEVNIDY 180 Query: 181 PEHDVESLTAEFIKDKSSEVMEGINQLLKTANEGKILREGITTAIVGRPNVGKSSLLNAL 240 PE+D E++T + +KD++ V + I++LL TA +GKILREG++T I+GRPNVGKSSLLN+L Sbjct: 181 PEYDAETMTLDMLKDRAQFVRKEIDKLLMTAQQGKILREGLSTVIIGRPNVGKSSLLNSL 240 Query: 241 ARDNKAIVTDIPGTTRDVIEEYVTINNIPLKLLDTAGIRETMDVVEKIGVERSKAAFSDA 300 +NKAIVTDIPGTTRDVIEEYV + +PL+L+DTAGIRET D+VE+IGVERS+ +A Sbjct: 241 VHENKAIVTDIPGTTRDVIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVERSREVLKEA 300 Query: 301 DLILLVLNAADELHEDELALMEQIQGRQALVIMNKMDLPSRLEKDKLLSYFSESSIVPMS 360 +LILLVLN +EL +++ AL E I G +A+VI+NK D+ +++ ++ + ++ S Sbjct: 301 ELILLVLNFGEELSKEDEALFEAISGMEAIVIVNKTDVEQKIDLTRVRELAAGRPVITTS 360 Query: 361 VLEEEGLDKLEEAISGLFFGGKLESGDLTYVSNVRHIALLKKAYKSLQDAYEAADMLIPI 420 +++++G+D+LE AI+ LFF +ESGD+TYVSN RHIALL++A ++++DA EA +M +PI Sbjct: 361 IVQDQGIDELEAAIASLFFESGIESGDVTYVSNSRHIALLEQAKRAVEDALEAIEMDVPI 420 Query: 421 DMIQIDVRLAWEQLGEIIGDTAADSLLDQIFSQFCLGK 458 DM+QID+ AWE LGEIIGD+ +SL+DQ+FSQFCLGK Sbjct: 421 DMVQIDITRAWELLGEIIGDSVHESLIDQLFSQFCLGK 458 >ref|NP_694413.1| tRNA modification GTPase [Oceanobacillus iheyensis HTE831] sp|Q8CX54|TRME_OCEIH tRNA modification GTPase trmE dbj|BAC15447.1| tRNA modification GTPase [Oceanobacillus iheyensis HTE831] Length = 459 Score = 553 bits (1425), Expect = e-156, Method: Compositional matrix adjust. Identities = 263/459 (57%), Positives = 365/459 (79%), Gaps = 2/459 (0%) Query: 1 MHSDTIAAVSTALGEGGIAIIRVSGPQAIAEVAPLFRSKTPLTEVESHTVHYGHIISPGD 60 + +DTI A+ST +GEG IAI+R+SG +AI + +F K L EV SHT+ YG II PG Sbjct: 2 LDTDTITAISTPVGEGAIAIVRLSGSEAITITSQIFEGKN-LQEVPSHTIQYGKIIDPGT 60 Query: 61 GERMEEVLVTVMKGPRSFTTEDVVEISAHGGVISVRRVMDLLLQRDIRLAEPGEFTKRAF 120 E EEV+V++M+GP++FT EDVVEI+ HGG+++V RV++++L + +RLAEPGEFTKRAF Sbjct: 61 NEVAEEVMVSIMRGPKTFTREDVVEINCHGGMVAVNRVLEIVLAKGVRLAEPGEFTKRAF 120 Query: 121 LGGRIDLSQAEAVIDLIRSKSDRAFSVALKQVSGSLSDKIHKLRHTLIEMLAHIEVNIDY 180 L GRIDLSQAEAV+DLIR+K+D+A SVALKQ+ G LS I KLR L+E +AH+EVNIDY Sbjct: 121 LHGRIDLSQAEAVMDLIRAKTDKAMSVALKQMDGRLSKLIQKLRQELLETVAHVEVNIDY 180 Query: 181 PEHD-VESLTAEFIKDKSSEVMEGINQLLKTANEGKILREGITTAIVGRPNVGKSSLLNA 239 PE+D VE ++ +K+KS EV + +++LL+ A +GKILREG++TAI+GRPNVGKSSL+N Sbjct: 181 PEYDDVEEMSHAMMKEKSKEVRDELDKLLQIAQQGKILREGLSTAIIGRPNVGKSSLMNT 240 Query: 240 LARDNKAIVTDIPGTTRDVIEEYVTINNIPLKLLDTAGIRETMDVVEKIGVERSKAAFSD 299 L ++NKAIVT+IPGTTRD+IEEYV + +PL+L+DTAGIRET D+VE+IGV+RS+ + Sbjct: 241 LVQENKAIVTEIPGTTRDIIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVDRSRQVLKE 300 Query: 300 ADLILLVLNAADELHEDELALMEQIQGRQALVIMNKMDLPSRLEKDKLLSYFSESSIVPM 359 +DLIL VLN + L ED+ L E + G + +VI+NK DL +L+ D++ + + +V Sbjct: 301 SDLILFVLNYNEPLSEDDKKLFEAVDGLEYIVIINKTDLEQQLDLDEVREFAKDRPVVTT 360 Query: 360 SVLEEEGLDKLEEAISGLFFGGKLESGDLTYVSNVRHIALLKKAYKSLQDAYEAADMLIP 419 ++LEE+G+D+LE+AI+ FF G +++GD+TYVSNVRHI LLK+A ++L+DA E ++ +P Sbjct: 361 ALLEEQGVDELEKAIADTFFTGDIDTGDMTYVSNVRHIQLLKQAKQALEDAMEGIELGMP 420 Query: 420 IDMIQIDVRLAWEQLGEIIGDTAADSLLDQIFSQFCLGK 458 +D++QIDV +WE +GEIIGDTA+DSL+DQ+FSQFCLGK Sbjct: 421 MDIVQIDVTRSWEFMGEIIGDTASDSLIDQLFSQFCLGK 459 >ref|NP_765972.1| putative thiophene and furan oxidation protein [Staphylococcus epidermidis ATCC 12228] sp|Q8CMN5|TRME_STAEP tRNA modification GTPase trmE gb|AAO06060.1| putative thiophene and furan oxidation protein [Staphylococcus epidermidis ATCC 12228] Length = 459 Score = 545 bits (1403), Expect = e-153, Method: Compositional matrix adjust. Identities = 258/459 (56%), Positives = 356/459 (77%), Gaps = 1/459 (0%) Query: 1 MHSDTIAAVSTALGEGGIAIIRVSGPQAIAEVAPLFRSKTPLTEVESHTVHYGHIISPGD 60 M DTI ++ST +GEG I I+R+SGPQAI L++ K L+EVE+HT++YGHII P Sbjct: 1 MDFDTITSISTPMGEGAIGIVRLSGPQAIEIGDILYKGKKKLSEVETHTINYGHIIDPET 60 Query: 61 GERMEEVLVTVMKGPRSFTTEDVVEISAHGGVISVRRVMDLLLQRDIRLAEPGEFTKRAF 120 E +EEV+V+V++ P++FT ED++EI+ HGG++++ R+++L + R+AEPGE+TKRAF Sbjct: 61 NETVEEVMVSVLRAPKTFTREDIIEINCHGGILTINRILELTMTYGARMAEPGEYTKRAF 120 Query: 121 LGGRIDLSQAEAVIDLIRSKSDRAFSVALKQVSGSLSDKIHKLRHTLIEMLAHIEVNIDY 180 L GRIDLSQAEAV+D IRSK+DRA VA+ Q+ G LSD I K R +++E+LA +EVNIDY Sbjct: 121 LNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLIKKQRQSILEILAQVEVNIDY 180 Query: 181 PEHD-VESLTAEFIKDKSSEVMEGINQLLKTANEGKILREGITTAIVGRPNVGKSSLLNA 239 PE+D VE T +F+ ++S + E INQLL+T +GKI+REG++T IVGRPNVGKSS+LN Sbjct: 181 PEYDDVEDATTDFLLEQSKRIKEEINQLLETGAQGKIMREGLSTVIVGRPNVGKSSMLNN 240 Query: 240 LARDNKAIVTDIPGTTRDVIEEYVTINNIPLKLLDTAGIRETMDVVEKIGVERSKAAFSD 299 L +DNKAIVT++ GTTRDV+EEYV + +PL+L+DTAGIR+T D+VEKIGVERS+ A S+ Sbjct: 241 LIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDTAGIRDTEDIVEKIGVERSRKALSE 300 Query: 300 ADLILLVLNAADELHEDELALMEQIQGRQALVIMNKMDLPSRLEKDKLLSYFSESSIVPM 359 ADLIL VLN + L ED+ L E I+ +VI+NK DL RL+ +L + ++ Sbjct: 301 ADLILFVLNNNEPLTEDDQTLFEVIKNEDVIVIINKTDLEQRLDVSELREMIGDMPLIQT 360 Query: 360 SVLEEEGLDKLEEAISGLFFGGKLESGDLTYVSNVRHIALLKKAYKSLQDAYEAADMLIP 419 S+L++EG+D+LE I LFFGG++++ D+TYVSN RHI+LLK+A +S+QDA +AA+ IP Sbjct: 361 SMLKQEGIDELEIQIKDLFFGGEVQNQDMTYVSNSRHISLLKQARQSIQDAIDAAESGIP 420 Query: 420 IDMIQIDVRLAWEQLGEIIGDTAADSLLDQIFSQFCLGK 458 +DM+QID+ WE LGEIIG++A+D L+DQ+FSQFCLGK Sbjct: 421 MDMVQIDLTRTWEILGEIIGESASDELIDQLFSQFCLGK 459 >ref|NP_653951.1| MMR_HSR1, GTPase of unknown function [Bacillus anthracis A2012] ref|NP_847879.1| tRNA modification GTPase TrmE [Bacillus anthracis str. Ames] gb|AAP29365.1| tRNA modification GTPase TrmE [Bacillus anthracis str. Ames] Length = 458 Score = 540 bits (1391), Expect = e-152, Method: Compositional matrix adjust. Identities = 262/459 (57%), Positives = 357/459 (77%), Gaps = 2/459 (0%) Query: 1 MHSDTIAAVSTALGEGGIAIIRVSGPQAIAEVAPLFRSKTPLTEVESHTVHYGHIISPGD 60 M DTIAA+STALGEG IAI+RVSG A+ +V +F+ K LTEV SHT+HYGHI+ Sbjct: 1 MEFDTIAAISTALGEGAIAIVRVSGDDAVEKVNRIFKGK-DLTEVPSHTIHYGHIVDLDT 59 Query: 61 GERMEEVLVTVMKGPRSFTTEDVVEISAHGGVISVRRVMDLLLQRDIRLAEPGEFTKRAF 120 + +EEV+V++M+ PR+FT E++VEI+ HGG++SV +V+ L+L + +RLAEPGEFTKRAF Sbjct: 60 NQVIEEVMVSIMRAPRTFTRENIVEINCHGGLVSVNKVLQLILAQGVRLAEPGEFTKRAF 119 Query: 121 LGGRIDLSQAEAVIDLIRSKSDRAFSVALKQVSGSLSDKIHKLRHTLIEMLAHIEVNIDY 180 L GRIDLSQAEAV+DLIR+K+DRA +VA+ Q+ G LS I +LR ++E LAH+EVNIDY Sbjct: 120 LNGRIDLSQAEAVMDLIRAKTDRAMNVAINQMEGRLSKLIGRLRQDILETLAHVEVNIDY 179 Query: 181 PEHD-VESLTAEFIKDKSSEVMEGINQLLKTANEGKILREGITTAIVGRPNVGKSSLLNA 239 PE+D VE +T + +K++ V I ++L+T+ +GKILREGI TAI+GRPNVGKSSLLN+ Sbjct: 180 PEYDDVEEMTHNILIEKATHVRAEIAKILETSKQGKILREGIATAIIGRPNVGKSSLLNS 239 Query: 240 LARDNKAIVTDIPGTTRDVIEEYVTINNIPLKLLDTAGIRETMDVVEKIGVERSKAAFSD 299 L ++ KAIVTDI GTTRDVIEEYV + +PLKL+DTAGIRET DVVE+IGVERSK S Sbjct: 240 LVQEKKAIVTDIAGTTRDVIEEYVNVRGVPLKLIDTAGIRETEDVVERIGVERSKEMMSQ 299 Query: 300 ADLILLVLNAADELHEDELALMEQIQGRQALVIMNKMDLPSRLEKDKLLSYFSESSIVPM 359 ADL+L+V+N ++ L ++ L +QG+ +VI+NK DLP ++ ++++ + + ++ Sbjct: 300 ADLVLVVVNYSETLTNEDEELFRAVQGKDFIVIVNKTDLPQAIDMERVIELAAGNRVITT 359 Query: 360 SVLEEEGLDKLEEAISGLFFGGKLESGDLTYVSNVRHIALLKKAYKSLQDAYEAADMLIP 419 S++EE+G+D+LE+AI+ LFF G ++S D+TYVSN RHI LL +A K++ DA EA + +P Sbjct: 360 SLIEEQGIDELEKAIADLFFEGTIDSADVTYVSNARHIGLLTQAGKTIGDAIEAIENGVP 419 Query: 420 IDMIQIDVRLAWEQLGEIIGDTAADSLLDQIFSQFCLGK 458 IDM+QID+ WE LGEI GDT +SL+DQ+FSQFCLGK Sbjct: 420 IDMVQIDLTRTWEILGEITGDTVHESLIDQLFSQFCLGK 458 >ref|NP_981927.1| tRNA modification GTPase TrmE [Bacillus cereus ATCC 10987] gb|AAS44535.1| tRNA modification GTPase TrmE [Bacillus cereus ATCC 10987] Length = 458 Score = 539 bits (1389), Expect = e-152, Method: Compositional matrix adjust. Identities = 263/459 (57%), Positives = 356/459 (77%), Gaps = 2/459 (0%) Query: 1 MHSDTIAAVSTALGEGGIAIIRVSGPQAIAEVAPLFRSKTPLTEVESHTVHYGHIISPGD 60 M DTIAA+STALGEG IAI+RVSG A+ +V +F+ K LTEV SHT+HYGHI+ Sbjct: 1 MEFDTIAAISTALGEGAIAIVRVSGDDAVEKVNRIFKGK-DLTEVPSHTIHYGHIVDLDT 59 Query: 61 GERMEEVLVTVMKGPRSFTTEDVVEISAHGGVISVRRVMDLLLQRDIRLAEPGEFTKRAF 120 + +EEV+V++M+ PR+FT E++VEI+ HGG++SV +V+ L+L + +RLAEPGEFTKRAF Sbjct: 60 NQVIEEVMVSIMRAPRTFTRENIVEINCHGGLVSVNKVLQLILAQGVRLAEPGEFTKRAF 119 Query: 121 LGGRIDLSQAEAVIDLIRSKSDRAFSVALKQVSGSLSDKIHKLRHTLIEMLAHIEVNIDY 180 L GRIDLSQAEAV+DLIR+K+DRA +VA+ Q+ G LS I +LR ++E LAH+EVNIDY Sbjct: 120 LNGRIDLSQAEAVMDLIRAKTDRAMNVAINQMEGRLSKLIGRLRQDILETLAHVEVNIDY 179 Query: 181 PEHD-VESLTAEFIKDKSSEVMEGINQLLKTANEGKILREGITTAIVGRPNVGKSSLLNA 239 PE+D VE +T + +K++ V I ++L+T+ +GKILREGI TAI+GRPNVGKSSLLN+ Sbjct: 180 PEYDDVEEMTHNILIEKATHVRAEIAKILETSKQGKILREGIATAIIGRPNVGKSSLLNS 239 Query: 240 LARDNKAIVTDIPGTTRDVIEEYVTINNIPLKLLDTAGIRETMDVVEKIGVERSKAAFSD 299 L ++ KAIVTDI GTTRDVIEEYV + +PLKL+DTAGIRET DVVE+IGVERSK S Sbjct: 240 LVQEKKAIVTDIAGTTRDVIEEYVNVRGVPLKLIDTAGIRETEDVVERIGVERSKEMMSQ 299 Query: 300 ADLILLVLNAADELHEDELALMEQIQGRQALVIMNKMDLPSRLEKDKLLSYFSESSIVPM 359 ADL+L+V+N ++ L ++ L +QG+ +VI+NK DLP ++ ++++ + + I+ Sbjct: 300 ADLVLVVVNYSEALTNEDEDLFRAVQGKDFIVIVNKTDLPQAIDMERVIELAAGNRIITT 359 Query: 360 SVLEEEGLDKLEEAISGLFFGGKLESGDLTYVSNVRHIALLKKAYKSLQDAYEAADMLIP 419 S++EE+G+D+LE AI+ LFF G ++S D+TYVSN RHI LL +A K++ DA EA + +P Sbjct: 360 SLIEEQGIDELETAIADLFFEGTIDSADVTYVSNARHIGLLTQAGKTIGDAIEAIENGVP 419 Query: 420 IDMIQIDVRLAWEQLGEIIGDTAADSLLDQIFSQFCLGK 458 IDM+QID+ WE LGEI GDT +SL+DQ+FSQFCLGK Sbjct: 420 IDMVQIDLTRTWEILGEITGDTVHESLIDQLFSQFCLGK 458 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.135 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 603,487,291 Number of extensions: 24299960 Number of successful extensions: 104363 Number of sequences better than 1.0e-05: 1640 Number of HSP's gapped: 102774 Number of HSP's successfully gapped: 1871 Length of query: 458 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 329 Effective length of database: 406,989,807 Effective search space: 133899646503 Effective search space used: 133899646503 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)