BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0015.p (187 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_350308.1| Hypothetical protein [Clostridium acetobutylicu... 94 1e-18 ref|ZP_00059737.1| hypothetical protein [Clostridium thermocellu... 94 2e-18 ref|NP_780819.1| conserved protein [Clostridium tetani E88] >gi|... 85 8e-16 ref|NP_624288.1| hypothetical protein [Thermoanaerobacter tengco... 81 1e-14 ref|NP_563566.1| hypothetical protein [Clostridium perfringens] ... 80 2e-14 >ref|NP_350308.1| Hypothetical protein [Clostridium acetobutylicum] pir||E97357 hypothetical protein CAC3728 [imported] - Clostridium acetobutylicum gb|AAK81648.1| Hypothetical protein [Clostridium acetobutylicum] Length = 169 Score = 94.0 bits (232), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 48/123 (39%), Positives = 78/123 (63%) Query: 58 IRKMRRKYEAMMNGNGIENLESLLIDLKNQGDMLEEVQREQKTIIEAAQVKLRGMKSKVA 117 ++K++R Y +M G+ ENLE L+ ++ + + E ++ K I E Q +++ VA Sbjct: 37 MKKLKRDYRKLMRGSNAENLEELINGYLDKVEKINEDSKQVKDIYEELQDQVKKCVKNVA 96 Query: 118 VKRYNAFGERGNDLSFSLAIIDDNRSGVVLTSLHNRENSYIYSKPLEGGESQYPLSPEEK 177 + RY AF G+DLSFSL ++DDN G++LTS++ R S +Y+KP+ G S+Y LS EEK Sbjct: 97 MVRYKAFENIGSDLSFSLVLLDDNYDGIMLTSIYGRGESVVYAKPINKGLSRYDLSEEEK 156 Query: 178 EVI 180 ++ Sbjct: 157 NIL 159 >ref|ZP_00059737.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 169 Score = 93.6 bits (231), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 49/136 (36%), Positives = 87/136 (63%) Query: 52 MIQGTKIRKMRRKYEAMMNGNGIENLESLLIDLKNQGDMLEEVQREQKTIIEAAQVKLRG 111 + K+++++RKY+ MNG+ +N+E LL+D + + +E + + + + L+ Sbjct: 33 FVTNRKLKELKRKYKKFMNGSDNKNIEELLLDNIKLSNDISLKNKEIENRVMSLERDLQR 92 Query: 112 MKSKVAVKRYNAFGERGNDLSFSLAIIDDNRSGVVLTSLHNRENSYIYSKPLEGGESQYP 171 KV V RYNAF + G+DLSFS+A++DDN +GVV++ +++RE+S Y+K + G S+ P Sbjct: 93 CIQKVGVVRYNAFDDVGSDLSFSIALLDDNDNGVVISGIYSRESSTTYAKKIVNGTSKNP 152 Query: 172 LSPEEKEVIALASQQN 187 LS EE + I+ + + N Sbjct: 153 LSAEELQAISESKKIN 168 >ref|NP_780819.1| conserved protein [Clostridium tetani E88] gb|AAO34756.1| conserved protein [Clostridium tetani E88] Length = 165 Score = 85.1 bits (209), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 47/159 (29%), Positives = 83/159 (52%) Query: 22 MSELNEIISEQLSMFIMGFXXXXXXXXXXXMIQGTKIRKMRRKYEAMMNGNGIENLESLL 81 M E +I ++G ++Q I ++ ++Y M G +NLE L+ Sbjct: 1 MQEFIQIFQNYSVFIVLGLIIATIILFFIVVVQAKAINRLEKRYRKFMRGVDNKNLEELI 60 Query: 82 IDLKNQGDMLEEVQREQKTIIEAAQVKLRGMKSKVAVKRYNAFGERGNDLSFSLAIIDDN 141 ++ D E + K + ++ + +L K+A+ RY AF + G+DLSFS+A++D Sbjct: 61 NTYLDKVDKASEECQYAKELYKSLEDRLNICVQKIAIIRYRAFEDVGSDLSFSVALLDYK 120 Query: 142 RSGVVLTSLHNRENSYIYSKPLEGGESQYPLSPEEKEVI 180 SG+++T ++ R S Y+KP++ G S+Y LS EE V+ Sbjct: 121 DSGIIITGIYGRNESTTYAKPIDKGISRYELSEEENHVL 159 >ref|NP_624288.1| hypothetical protein [Thermoanaerobacter tengcongensis] gb|AAM25892.1| hypothetical protein [Thermoanaerobacter tengcongensis] Length = 167 Score = 80.9 bits (198), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 52/178 (29%), Positives = 88/178 (49%), Gaps = 26/178 (14%) Query: 22 MSELNEIISEQLSMFIMGFXXXXXXXXXXXMIQGTKIRKMRRKYEAMMNGNGIENLESLL 81 M E++ L +F + +I K K++R YE + Sbjct: 1 MQNFLELVYSNLPLFTLALAVLAIFELILILILNAKFLKIKRTYEKV------------- 47 Query: 82 IDLKNQGDMLEEVQR------EQKTIIEAAQVKLRGMKS-------KVAVKRYNAFGERG 128 ID +GD+ + +QR E + I +VK+ ++ KV + RYNAF + G Sbjct: 48 IDTLQKGDIFDLLQRILVEKEEIRNDINQIRVKVNALEKEGRRAIKKVGIVRYNAFPDVG 107 Query: 129 NDLSFSLAIIDDNRSGVVLTSLHNRENSYIYSKPLEGGESQYPLSPEEKEVIALASQQ 186 +DLS+S+A +D+ +GVVL+ ++ R + ++KP+E G S+YPLS EE + I A ++ Sbjct: 108 SDLSYSIAFLDEEDNGVVLSGIYGRNETATFAKPIERGHSKYPLSAEEVQAIERAKRK 165 >ref|NP_563566.1| hypothetical protein [Clostridium perfringens] dbj|BAB82356.1| hypothetical protein [Clostridium perfringens str. 13] Length = 165 Score = 80.5 bits (197), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 46/129 (35%), Positives = 79/129 (61%), Gaps = 6/129 (4%) Query: 58 IRKMRRKYEAMMNGNGIENLESLLIDLKNQGDMLEEVQREQKTIIEAAQV---KLRGMKS 114 + K+ K+ +M G +NLE L+ + D +EEV+++ + +E ++ ++ Sbjct: 37 LSKVENKFRKIMRGVNNKNLEELI---NSYLDKVEEVKKDSEETLETNKILKAQVEKCTQ 93 Query: 115 KVAVKRYNAFGERGNDLSFSLAIIDDNRSGVVLTSLHNRENSYIYSKPLEGGESQYPLSP 174 KV+V RY AF + G+DLSFS+A++D +GV+LT ++ R+ S Y+KP++ G S+Y LS Sbjct: 94 KVSVIRYKAFEDVGSDLSFSVALLDGENNGVILTGIYGRDYSTTYAKPIDKGISRYDLSE 153 Query: 175 EEKEVIALA 183 EE V+ A Sbjct: 154 EELHVLNAA 162 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.314 0.131 0.349 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 232,796,953 Number of extensions: 8090670 Number of successful extensions: 19654 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 19656 Number of HSP's successfully gapped: 7 Length of query: 187 Length of database: 757,237,578 Length adjustment: 118 Effective length of query: 69 Effective length of database: 436,855,896 Effective search space: 30143056824 Effective search space used: 30143056824 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 122 (51.6 bits)