BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0003.p (363 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_623525.1| predicted dehydrogenases and related proteins [... 198 2e-49 ref|NP_243031.1| BH2165~unknown conserved protein [Bacillus halo... 172 2e-41 ref|NP_694393.1| NADH-dependent dehydrogenase [Oceanobacillus ih... 152 1e-35 ref|NP_370741.1| hypothetical protein [Staphylococcus aureus sub... 150 4e-35 gb|AAO61215.1| oxidoreductase [Streptomyces hygroscopicus] 150 5e-35 >ref|NP_623525.1| predicted dehydrogenases and related proteins [Thermoanaerobacter tengcongensis] gb|AAM25129.1| predicted dehydrogenases and related proteins [Thermoanaerobacter tengcongensis] Length = 348 Score = 198 bits (504), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 115/356 (32%), Positives = 181/356 (50%), Gaps = 16/356 (4%) Query: 8 KVISIGMIGAGWVAEHGHLPGFLSLEGVSIGAVYDPDASRAAHLAEQAGAKACTSLSE-- 65 K + +GAG VA++ HL G+ L+ V I A+ DPD A LAE+ E Sbjct: 2 KKLRYACVGAGAVADYKHLLGYSRLKDVEIVAISDPDVGAAKRLAEKYSIPHVYENYEEM 61 Query: 66 IDEIPLDAVLVCTPNHTHYELAKHFLQQGIHVLCEKPMTTTAEEAGSLRRIAESSGAVLL 125 ++ LD + +CTPN+ H ++ +++GIH+ CEKPMT A+EA + + Sbjct: 62 FEKEKLDLISICTPNYLHAPISIKAMEKGIHIHCEKPMTLNAKEAHKVIEAKNKYKTKFM 121 Query: 126 MGFVNRYRDDIRELRSRITSGQIGDVQICEVIWRRKKGIPRPGSWFTQQKMSGGGVLIDL 185 +G NR+ ++ ++ I G +G++ + WRR++GIP G WFT +K+SGGG LIDL Sbjct: 122 VGLNNRFTNESYFVKKYIEEGYLGEIYHVKTGWRRRRGIPGKGGWFTTKKLSGGGPLIDL 181 Query: 186 GSHMIDQMLFLTDAPEPVACLAGLHSRPAQEGDYSNWLGSNESVTL-EVEDTAIGMIQFA 244 G H ID +++ D PE + A +S+ + ++ W + + VED A+G I+ Sbjct: 182 GVHFIDLVMYFMDYPEVKSVSAATYSKFSNSTSWNGWSEAKSGNGIYNVEDMAVGFIRLK 241 Query: 245 NGMLGRIHVSWQDDVEGDFVEIHLKGSRGSLSLRTLFGFSNQGLYKQPSLQFTPAGQEPE 304 NG SW ++EGD+ + G +G + F N + + T P Sbjct: 242 NGTTIDFEFSWASNIEGDYNYYEILGDKGGI------WFKNGQIKVFSEVVNTLVDIIPN 295 Query: 305 WFTFPPKTDVLIDFRRQASHFAECIREAKQPDITAEDGEKAIRLIQLLYQSAHKGL 360 +P K L +F HF CI+E K+P +AE+G K + +I Y SA KG+ Sbjct: 296 -TNYPKKP--LDEFE----HFVNCIKEGKEPLASAEEGAKLMEIIDAAYLSAEKGV 344 >ref|NP_243031.1| BH2165~unknown conserved protein [Bacillus halodurans] pir||E83920 hypothetical protein BH2165 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05884.1| BH2165~unknown conserved protein [Bacillus halodurans] Length = 348 Score = 172 bits (435), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 120/357 (33%), Positives = 175/357 (49%), Gaps = 22/357 (6%) Query: 8 KVISIGMIGAGWVAEHGHLPGFLSLEG-VSIGAVYDPDASRAAHLAEQAGAKACTSLSE- 65 K + +G+IGAG +A H+P +LSL+ V + A+ D A AEQ + + Sbjct: 4 KKLRVGIIGAGGIARTVHIPSYLSLKDQVELVAITDVQPKHAQAAAEQFSIPYVYNDASR 63 Query: 66 -IDEIPLDAVLVCTPNHTHYELAKHFLQQGIHVLCEKPMTTTAEEAGSLRRIAESSGAVL 124 + E+ LDAV +CTPN H EL L G HVLCEKP T E+A + A+ +G +L Sbjct: 64 MMQEVELDAVSICTPNAFHRELTIEALHAGCHVLCEKPPAITVEDAKQMEAAAKETGNLL 123 Query: 125 LMGFVNRYRDDIRELRSRITSGQIGDVQICEVIWRRKKGIPRPGSWFTQQKMSGGGVLID 184 GF R+ ++ L+ + G++G++ V R++GIP G FT +++ GGG LID Sbjct: 124 TYGFHYRHSPEVETLKRFVDEGELGEIYHVRVQAIRRRGIPGWGV-FTNKELQGGGPLID 182 Query: 185 LGSHMIDQMLFLTDAPEPVACLAGLHSR-PAQEGDYSNWLGSNESVTLEVEDTAIGMIQF 243 +G HM+D L++ PEP L H + +EG LG +ED A+GMI F Sbjct: 183 IGVHMLDTALYVMGFPEPATILGQTHRKIGTKEG--VGLLGEWNWQQFSIEDMAVGMITF 240 Query: 244 ANGMLGRIHVSWQDDV-EGDFVEIHLKGSRGSLSLRTLFGFSNQGLYKQPSLQFTPAGQE 302 NG I ++ +V E D + + G +G L L + + + L TP Sbjct: 241 KNGASLTIETAFAANVKEQDHMNVSFFGDKGGADLFPLSIYQEK---HRTLLDITP---- 293 Query: 303 PEWFTFPPKTDVLIDFRRQASHFAECIREAKQPDITAEDGEKAIRLIQLLYQSAHKG 359 + P + R+ HF +C +QP T E G RLIQ LYQSA G Sbjct: 294 ----VYLPTYN---HHEREIKHFVDCCFRLEQPLATPEQGIVIQRLIQGLYQSAESG 343 >ref|NP_694393.1| NADH-dependent dehydrogenase [Oceanobacillus iheyensis HTE831] dbj|BAC15427.1| NADH-dependent dehydrogenase [Oceanobacillus iheyensis HTE831] Length = 360 Score = 152 bits (384), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 100/358 (27%), Positives = 172/358 (48%), Gaps = 10/358 (2%) Query: 7 TKVISIGMIGAGWVAEHGHLPGFLSLEGVSIGAVYDPDASRAAHLAEQAGAKACTSLSE- 65 T+ I +G+IG G +A HLP L V I A D D +A A++ G + + Sbjct: 2 TRTIQVGIIGCGGIAFGKHLPSLSKLNHVKIVAFCDIDREKAERAAKEYGGEDAVIYEDY 61 Query: 66 ---IDEIPLDAVLVCTPNHTHYELAKHFLQQGIHVLCEKPMTTTAEEAGSLRRIAESSGA 122 I++ ++ V VCTPN++H E++ L G HV+CEKPM TAEEA ++ AE Sbjct: 62 SQLIEQSSVEVVHVCTPNNSHAEISIAALHGGKHVMCEKPMAKTAEEAKAMIDAAEKCNK 121 Query: 123 VLLMGFVNRYRDDIRELRSRITSGQIGDVQICEVIWRRKKGIPRPGSWFTQQKMSGGGVL 182 L +G+ NR+R D + L+ G +G + + R++ +P G + ++K GGG L Sbjct: 122 KLTIGYNNRFRTDSQYLKKVCQRGDLGHIYFAKAHAIRRRAVPTWGVFLDEEK-QGGGPL 180 Query: 183 IDLGSHMIDQMLFLTDAPEPVACLAGLHSRPAQEGDYSNWLGSNESVTLEVEDTAIGMIQ 242 ID+G+H +D L++ D EP + + H ++ D +N G+ VED A G I Sbjct: 181 IDIGTHALDLTLWMMDNYEPKSVMGTTHHLLGKKKDAANPWGTWNPNEFTVEDAAFGFIT 240 Query: 243 FANGMLGRIHVSWQ-DDVEGDFVEIHLKGSRGSLSLRTLFGFSNQGLYKQPSLQFTPAGQ 301 NG + SW + ++ D + L G+ G ++ + L + + Q Sbjct: 241 MKNGATIILESSWALNSLDVDEAKCSLSGTEGGADMKDGLRLHGEDLGELYTKQVDLGPG 300 Query: 302 EPEWFTFPPKTDVLIDFRRQASHFAECIREAKQPDITAEDGEKAIRLIQLLYQSAHKG 359 ++ ++D ++ R + E I K+ + ++ ++++ +YQSA G Sbjct: 301 GVAFYDGNTESDAELEARL----WIESIINEKEATVQPKEALVVTQILEAIYQSAKTG 354 >ref|NP_370741.1| hypothetical protein [Staphylococcus aureus subsp. aureus Mu50] dbj|BAB56379.1| hypothetical protein [Staphylococcus aureus subsp. aureus Mu50] Length = 359 Score = 150 bits (379), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 98/356 (27%), Positives = 168/356 (47%), Gaps = 10/356 (2%) Query: 9 VISIGMIGAGWVAEHGHLPGFLSLEGVSIGAVYDPDASRAAHLAEQAGAKACTSLSE--- 65 I +G+IG G +A H+P +E V + A D D S+AA AE G + Sbjct: 3 TIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASAAEAYGTDNAKVYDDYKA 62 Query: 66 -IDEIPLDAVLVCTPNHTHYELAKHFLQQGIHVLCEKPMTTTAEEAGSLRRIAESSGAVL 124 + + +D + VCTPN +H E+ L G HV+CEKPM T EA + A+S+G L Sbjct: 63 LLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAKTTAEAQKMIDTAKSTGKKL 122 Query: 125 LMGFVNRYRDDIRELRSRITSGQIGDVQICEVIWRRKKGIPRPGSWFTQQKMSGGGVLID 184 +G+ NR+R D + L G +GD+ + R++ +P G F ++ GGG LID Sbjct: 123 TIGYQNRFRPDSQFLHQAAQRGDLGDIYFGKAHAIRRRAVPTWGV-FLNEEAQGGGPLID 181 Query: 185 LGSHMIDQMLFLTDAPEPVACLAGLHSRPAQEGDYSNWLGSNESVTLEVEDTAIGMIQFA 244 +G+H +D L++ D EP + ++ + ++ D +N GS VED+A G I+ Sbjct: 182 IGTHALDLTLWMMDNYEPESVMSSTFHKLNKQHDAANAWGSWNPDEFTVEDSAFGFIKMK 241 Query: 245 NGMLGRIHVSWQ-DDVEGDFVEIHLKGSRGSLSLRTLFGFSNQGLYKQPSLQFTPAGQEP 303 NG + +W + +E D + L G++ ++ + + +L E Sbjct: 242 NGATIILESAWAINSLEVDEAKCSLSGTKAGADMKDGLRIHGEDM---GTLYTKHVELEN 298 Query: 304 EWFTFPPKTDVLIDFRRQASHFAECIREAKQPDITAEDGEKAIRLIQLLYQSAHKG 359 + F +V + +A + + + +P + E ++++ +YQSA G Sbjct: 299 KGVDFYEGNEV-DEAEEEAKAWIDAVVNDTEPVVKPEQAMVVTKILEAIYQSAKSG 353 >gb|AAO61215.1| oxidoreductase [Streptomyces hygroscopicus] Length = 377 Score = 150 bits (378), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 113/362 (31%), Positives = 167/362 (46%), Gaps = 20/362 (5%) Query: 10 ISIGMIGAGWVAEHGHLPGFLSLEGVSIGAVYDPDASRAAHLAEQAGAKACTSLS---EI 66 I ++G GW A LP ++ A DPD A +AE G L+ ++ Sbjct: 11 IRTAVVGLGWAARSIWLPRLRHNPAFTVTAAVDPDERGRAAVAEAEGMDRLPVLAAVHDL 70 Query: 67 DEIPLDAVLVCTPNHTHYELAKHFLQQGIHVLCEKPMTTTAEEAGSLRRIAESSGAVLLM 126 D +D +V PNH H +A L +GI V EKP+ T+EEA L S GA+LL Sbjct: 71 DPAEVDLAVVAVPNHLHCAVAAELLAKGIPVFLEKPVCLTSEEAERLAEAERSGGAMLLA 130 Query: 127 GFVNRYRDDIRELRSRITSGQIGDVQICEVIWRRKKGIPRPGSWFTQQKMSGGGVLIDLG 186 G RYR D+R L RI + ++G ++ E+ W R +G+P G WFTQ+ ++GGG L+DLG Sbjct: 131 GSAARYRADVRGL-YRIAA-RLGHIRHVELAWVRSRGVPDRGGWFTQRSLAGGGALVDLG 188 Query: 187 SHMIDQMLFL-----------TDAPEPVACLAGLHSRPAQEGDYSNWLGSNESVTLEVED 235 H+ D + L T + + + + + +GD LG+N T +VED Sbjct: 189 WHLFDIAVPLLGTAAFRHAIGTVSSDFIVQRSSRAAWRGDDGDGPALLGANGGAT-DVED 247 Query: 236 TAIGMIQFANGMLGRIHVSWQDDVEGDFVEIHLKGSRGSLSLRTLFGFSNQGLYKQPSLQ 295 TA G + +G +H SW E D + + GS GS +LR FGFS L K + Sbjct: 248 TARGFLITDDGRSVVLHASWASHEELDTTRVTIDGSAGSATLRCTFGFSPNRLEKSTLTR 307 Query: 296 FTPAGQEPEWFTFPPKTDVLIDFRRQASHFAECIREAKQPDITAEDGEKAIRLIQLLYQS 355 P P V ++ RQ +R+ ++ + I I+ +Y S Sbjct: 308 TVDGTTRP---VAVPTEPVGTEYDRQLDLLPAQLRDPAGRGRVIDEVRRTIGAIERVYAS 364 Query: 356 AH 357 A Sbjct: 365 AR 366 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 554,258,784 Number of extensions: 23134452 Number of successful extensions: 51511 Number of sequences better than 1.0e-05: 1251 Number of HSP's gapped: 50866 Number of HSP's successfully gapped: 1251 Length of query: 363 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 236 Effective length of database: 412,420,005 Effective search space: 97331121180 Effective search space used: 97331121180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)