BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0001.p (381 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||I39657 3-amino-5-hydroxybenzoic acid synthase (EC 4.2.1.-) ... 315 1e-84 gb|AAM54102.1| 3-amino-5-hydroxybenzoate synthase [Actinosynnema... 307 2e-82 gb|AAL77217.4| 3-amino-5-hydroxybenzoic acid synthase [Streptomy... 303 6e-81 gb|AAD27811.1| 3-amino-5-hydroxybenzoic acid synthase [Streptomy... 302 7e-81 gb|AAL48265.1| 3-amino-5-hydroxybenzoic acid synthase [Kitasatos... 298 1e-79 >pir||I39657 3-amino-5-hydroxybenzoic acid synthase (EC 4.2.1.-) - Actinosynnema pretiosum subsp. auranticum gb|AAC13997.1| 3-amino-5-hydroxybenzoic acid synthase [Actinosynnema pretiosum subsp. auranticum] Length = 388 Score = 315 bits (806), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 160/353 (45%), Positives = 220/353 (62%), Gaps = 14/353 (3%) Query: 17 NQWWRMTGTKVKEFERVFAEMHNAKYALGVTNGTNAIDLLLKSFKVKEGDEVIVPAFTFI 76 QWWR+ G++V EFER FAE H A +AL VTNGT+A++L L+ V G EVIVPAFTFI Sbjct: 32 GQWWRVGGSEVDEFEREFAEYHGAGHALAVTNGTHALELALQVLDVGPGTEVIVPAFTFI 91 Query: 77 STALPLMSLGAIPVPVDIDPGTFCLDPEKVREAVTERTVGIIPVHMAGHLCDMDRLLDIA 136 S++ + LGA+ VPVD+DP T+CLD +AVT RT I+PVHMAG DMDRL ++ Sbjct: 92 SSSQAVQRLGAVAVPVDVDPDTYCLDVAAAEDAVTSRTSAIMPVHMAGQFADMDRLDKLS 151 Query: 137 SEFNLFIMEDACHAHGGEWKGKRAGSIGDAGVFSFQQFKLMTAGEGGALITNSQELYDQA 196 + + +++DA HAHG W+GKR G +G FSFQ KLMTAGEGGA++ Q +++A Sbjct: 152 ASTGVPVVQDAAHAHGAHWRGKRVGELGSIATFSFQNGKLMTAGEGGAVLFADQAQWEKA 211 Query: 197 FLYHNVGRPFGDKKYQHLVEGTNYRFSEFQAAVLLPQAGRLAEQNQRRERNAQILNEEIQ 256 F+ H+ GRP GD+ Y HL G+N+R +EF AAVL Q GRL Q R+ +L+ + Sbjct: 212 FVLHSCGRPKGDRGYFHLTSGSNFRMNEFSAAVLRAQLGRLDSQIATRQARWPVLSALLA 271 Query: 257 TIEGIVPQGRLEACTLHTHYMYMFYYNPGKFGGISRER---FVEMLNEQGIPAYIAYAQI 313 I+G+VPQ + YM MF + G++ ER V+ L +GIPA++A+ + Sbjct: 272 GIDGVVPQTVDPRSDRNPSYMAMF-----RMPGVTEERRNAVVDELVRRGIPAFMAFRAV 326 Query: 314 HDTPIFKEFAASLDDSYSFPNQL--DCPASKKVAEEVIWIHHRALLGDEEATR 364 + T F E A P +L CP S+++ + +W+HHR LLG EE R Sbjct: 327 YRTQAFWETGA----PDLTPEELAARCPVSEEITRDCVWLHHRVLLGAEEQVR 375 >gb|AAM54102.1| 3-amino-5-hydroxybenzoate synthase [Actinosynnema pretiosum subsp. auranticum] Length = 388 Score = 307 bits (787), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 160/355 (45%), Positives = 226/355 (63%), Gaps = 4/355 (1%) Query: 16 SNQWWRMTGTKVKEFERVFAEMHNAKYALGVTNGTNAIDLLLKSFKVKEGDEVIVPAFTF 75 S +WWR+TG +V FER FA+ H A++AL VT GT+A++L+L+ G EVIVPAFTF Sbjct: 31 SGRWWRITGDEVVSFEREFAQAHGARHALAVTTGTHALELVLEVMGAGPGTEVIVPAFTF 90 Query: 76 ISTALPLMSLGAIPVPVDIDPGTFCLDPEKVREAVTERTVGIIPVHMAGHLCDMDRLLDI 135 IS++ LGA+ VPVD+DP T+C+DP VR A+TERT I+PVHMAG L DMD L + Sbjct: 91 ISSSQAAQRLGAVVVPVDVDPDTYCVDPAAVRAAITERTRAIMPVHMAGQLADMDALRAL 150 Query: 136 ASEFNLFIMEDACHAHGGEWKGKRAGSIGDAGVFSFQQFKLMTAGEGGALITNSQELYDQ 195 ++E + +++DA HAHG W+G+ G++G FSFQ KLMTAGEGGA++ + +++ Sbjct: 151 SAETGVPLLQDAAHAHGARWRGEPLGALGSIAAFSFQNGKLMTAGEGGAVLLPDGDQHEE 210 Query: 196 AFLYHNVGRPFGDKKYQHLVEGTNYRFSEFQAAVLLPQAGRLAEQNQRRERNAQILNEEI 255 AFL H GRP D+ Y H G+N+R +EF AA+L Q GRL +Q RER+ +L+ + Sbjct: 211 AFLRHACGRPLADRAYHHRTAGSNFRMNEFSAALLRAQLGRLQQQTDVRERHWPVLSRLL 270 Query: 256 QTIEGIVPQGRLEACTLHTHYMYMFYYNPGKFGGISRERFVEMLNEQGIPAYIAYAQIHD 315 + G+VPQG C + HYM MF PG G R R V+ L E+G+PA+ + I+ Sbjct: 271 AEVPGVVPQGTDPRCDRNPHYMAMFQV-PG-IGEERRARLVDALVERGVPAFAGFRAIYR 328 Query: 316 TPIFKEFAASLDDSYSFPNQLDCPASKKVAEEVIWIHHRALLGDEEATRGIAQTI 370 T F E AA D+ CP ++ ++ + +W+HHR LLG EE +A+ + Sbjct: 329 TAAFWERAAP--DTTVDALARACPVTEALSSDCVWLHHRVLLGAEEQMPLVAEAV 381 >gb|AAL77217.4| 3-amino-5-hydroxybenzoic acid synthase [Streptomyces hygroscopicus] Length = 427 Score = 303 bits (775), Expect = 6e-81, Method: Compositional matrix adjust. Identities = 163/367 (44%), Positives = 221/367 (60%), Gaps = 13/367 (3%) Query: 17 NQWWRMTGTKVKEFERVFAEMHNAKYALGVTNGTNAIDLLLKSFKVKEGDEVIVPAFTFI 76 QWWRM G++V FE FA+ H A +AL VTNGT+A++L L+ V G EVIVPAFTFI Sbjct: 71 GQWWRMGGSEVDSFEGEFADFHGAPHALAVTNGTHALELALQCLGVGPGTEVIVPAFTFI 130 Query: 77 STALPLMSLGAIPVPVDIDPGTFCLDPEKVREAVTERTVGIIPVHMAGHLCDMDRLLDIA 136 S++ LGA+ VPVD+D T+ +D AVT T I+PVHMAG + DMD L +++ Sbjct: 131 SSSQAAQRLGAVAVPVDVDLDTYNIDVAAAASAVTPLTKAIMPVHMAGLIADMDALGELS 190 Query: 137 SEFNLFIMEDACHAHGGEWKGKRAGSIGDAGVFSFQQFKLMTAGEGGALITNSQELYDQA 196 ++ + +++DA HAHG W+GKR G +G FSFQ KLMTAGEGGAL+ +E Y+ A Sbjct: 191 ADTGVPLLQDAAHAHGARWQGKRVGELGTVASFSFQNGKLMTAGEGGALLLPDEETYEAA 250 Query: 197 FLYHNVGRPFGDKKYQHLVEGTNYRFSEFQAAVLLPQAGRLAEQNQRRERNAQILNEEIQ 256 FL H+ GR D++Y H GTN R +EF AAVL Q GRL Q R++ +L+ + Sbjct: 251 FLRHSCGRSRTDRRYMHQTAGTNMRLNEFSAAVLRAQLGRLDAQITLRDQRWTLLSRLLG 310 Query: 257 TIEGIVPQGRLEACTLHTHYMYMFYYNPGKFGGIS---RERFVEMLNEQGIPAYIAYAQI 313 I+G+VPQG ++HYM MF + GIS R V+ L E G+PA+ A+ I Sbjct: 311 EIDGVVPQGSDPRADRNSHYMAMF-----RIPGISEEARNALVDTLVEAGLPAFAAFRAI 365 Query: 314 HDTPIFKEFAASLDDSYSFPNQLDCPASKKVAEEVIWIHHRALLGDEEATRGIAQTIREI 373 + T F E AA D+ CP ++ ++ + IW+HHR LL EEA A+ I + Sbjct: 366 YRTDAFWETAAP--DTTVDKLAESCPHTEAISTDCIWLHHRVLLASEEALHTTAEIIAD- 422 Query: 374 QAGVAAR 380 VAAR Sbjct: 423 --AVAAR 427 >gb|AAD27811.1| 3-amino-5-hydroxybenzoic acid synthase [Streptomyces lavendulae] gb|AAD32723.1| MitA [Streptomyces lavendulae] Length = 388 Score = 302 bits (774), Expect = 7e-81, Method: Compositional matrix adjust. Identities = 152/344 (44%), Positives = 211/344 (61%), Gaps = 11/344 (3%) Query: 19 WWRMTGTKVKEFERVFAEMHNAKYALGVTNGTNAIDLLLKSFKVKEGDEVIVPAFTFIST 78 WWR G +V FER FA+ H A +A+ TNGT+A++L L + GDEVIVPAFTFIS+ Sbjct: 35 WWRDAGQEVDLFEREFADHHGAPHAIATTNGTHALELALGVMGIGPGDEVIVPAFTFISS 94 Query: 79 ALPLMSLGAIPVPVDIDPGTFCLDPEKVREAVTERTVGIIPVHMAGHLCDMDRLLDIASE 138 +L + +GA+PVP D+ P T+CLD + VT RT I+PVHMAG DMD L ++ Sbjct: 95 SLAVQRMGAVPVPADVRPDTYCLDADAAAALVTPRTKAIMPVHMAGQFADMDALEKLSVA 154 Query: 139 FNLFIMEDACHAHGGEWKGKRAGSIGDAGVFSFQQFKLMTAGEGGALITNSQELYDQAFL 198 + +++DA HAHG +W+G+R G +G FSFQ KLMTAGEGGAL+ E + +AFL Sbjct: 155 TGVPVLQDAAHAHGAQWQGRRVGELGSIAAFSFQNGKLMTAGEGGALLLPDDESFHEAFL 214 Query: 199 YHNVGRPFGDKKYQHLVEGTNYRFSEFQAAVLLPQAGRLAEQNQRRERNAQILNEEIQTI 258 H GRP GD+ Y+HL +G+NYR +EF A+VL Q RL +Q + RE L + I Sbjct: 215 QHCCGRPPGDRVYRHLTQGSNYRMNEFSASVLRAQLKRLKDQLRIREERWAQLRTALAAI 274 Query: 259 EGIVPQGRLEACTLHTHYMYMFYYNPGKFGGISRER---FVEMLNEQGIPAYIAYAQIHD 315 +G+VPQGR E LH+HYM M + GIS R V+ L E+G+PA++ + ++ Sbjct: 275 DGVVPQGRDERGDLHSHYMAMV-----RLPGISARRRLALVDALVERGVPAFVGFPPVYR 329 Query: 316 TPIFKEFAASLDDSYSFPNQLDCPASKKVAEEVIWIHHRALLGD 359 T F A D + CP ++++ + +W+HHR LL D Sbjct: 330 TEGFARGPAPADAEELAKS---CPVAEEIGSDCLWLHHRVLLAD 370 >gb|AAL48265.1| 3-amino-5-hydroxybenzoic acid synthase [Kitasatospora sp. F18-97] Length = 388 Score = 298 bits (763), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 160/360 (44%), Positives = 213/360 (59%), Gaps = 4/360 (1%) Query: 18 QWWRMTGTKVKEFERVFAEMHNAKYALGVTNGTNAIDLLLKSFKVKEGDEVIVPAFTFIS 77 QWWRM G +V FE+ FA+ H A AL VTNGT+A+++ L+ V G EVIVPAFTFIS Sbjct: 33 QWWRMGGKEVDTFEQEFADYHGAAKALAVTNGTHALEIALQLLGVGPGTEVIVPAFTFIS 92 Query: 78 TALPLMSLGAIPVPVDIDPGTFCLDPEKVREAVTERTVGIIPVHMAGHLCDMDRLLDIAS 137 ++ LGA VPVDID T+ L PE A+T RT I+PVH+AG + D+D L ++A+ Sbjct: 93 SSQAAQRLGATAVPVDIDLDTYNLSPEAAAAAITPRTRAIMPVHIAGQIADLDALDELAA 152 Query: 138 EFNLFIMEDACHAHGGEWKGKRAGSIGDAGVFSFQQFKLMTAGEGGALITNSQELYDQAF 197 + + I++DA HAHG W+G+R G +G FSFQ KLMTAGEGGA+ YD+AF Sbjct: 153 DSGVSIVQDAAHAHGARWRGRRVGELGSIAAFSFQNGKLMTAGEGGAVTFPETAQYDEAF 212 Query: 198 LYHNVGRPFGDKKYQHLVEGTNYRFSEFQAAVLLPQAGRLAEQNQRRERNAQILNEEIQT 257 L H+ GRP D+KY H G+N+R +EF A+VL Q RL Q RE +++L + Sbjct: 213 LRHSCGRPPTDRKYFHQTSGSNFRLNEFSASVLRAQLARLDSQISVREERSKLLKSLLTE 272 Query: 258 IEGIVPQGRLEACTLHTHYMYMFYYNPGKFGGISRERFVEMLNEQGIPAYIAYAQIHDTP 317 I G+VPQG E HYM MF PG G R V+ L +G+PA++ + I+ + Sbjct: 273 IPGVVPQGHDERADRVPHYMAMFRV-PG-LGEDRRNALVDDLIARGLPAFVVFRAIYRSD 330 Query: 318 IFKEFAASLDDSYSFPNQLDCPASKKVAEEVIWIHHRALLGDEEATRGIAQTIREIQAGV 377 F E AA D+ CP S+ + + IW+HHR LL E A AQ I + A V Sbjct: 331 GFWESAAP--DTTVDELAKACPNSEALTADGIWLHHRTLLASEGAIEDTAQIIADALAAV 388 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.137 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 552,592,259 Number of extensions: 23421836 Number of successful extensions: 55087 Number of sequences better than 1.0e-05: 1554 Number of HSP's gapped: 53441 Number of HSP's successfully gapped: 1554 Length of query: 381 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 254 Effective length of database: 412,420,005 Effective search space: 104754681270 Effective search space used: 104754681270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)