BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0014.p (364 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00013768.1| COG0381: UDP-N-acetylglucosamine 2-epimerase ... 352 1e-95 gb|AAC46090.1| Cap5G [Staphylococcus aureus] 328 2e-88 ref|NP_644945.1| capsular polysaccharide synthesis enzyme Cap8G ... 327 4e-88 ref|NP_370679.1| capsular polysaccharide synthesis enzyme Cap5G ... 326 5e-88 gb|AAD12956.1| unknown [Leptospira borgpetersenii] 324 2e-87 >ref|ZP_00013768.1| COG0381: UDP-N-acetylglucosamine 2-epimerase [Rhodospirillum rubrum] Length = 365 Score = 352 bits (902), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 185/368 (50%), Positives = 245/368 (66%), Gaps = 10/368 (2%) Query: 1 MKIMTILGTRPEIIRLSVIIPLLDEHAEQHVLVHTGQNFTASLSGIFFAELGLRAPDYVL 60 MK+MTILGTRPE+IRLS+I+P LD + HV V+TGQN SL F E LR+ D+ L Sbjct: 1 MKVMTILGTRPELIRLSLILPRLDAVCD-HVFVYTGQNAHPSLWRNFLGEFSLRSEDHDL 59 Query: 61 QDKQAGLGGQLAAMFGSLESILLQEQPDRILLLGDTNSALCAILAERMGIPVVHMEAGNR 120 Q LG ++ A+ E ++ E+PDR+L+LGDTNS L A +A+R+GIPV HMEAGNR Sbjct: 60 GASQGTLGERVGAILAGAERVIGLERPDRVLILGDTNSGLSAYVAKRLGIPVFHMEAGNR 119 Query: 121 CYDLKVPEEKNRRVIDAVSTINMPYTQQSKRHLLSEGFPSQRIVLTGNPIHEVIKHYNRQ 180 C+D +VPEE NRRVID S + MPYT+ +R+LL+EG P QRIV+TGNPI EV+ + Sbjct: 120 CHDDRVPEEVNRRVIDHCSDLLMPYTEHGRRNLLAEGIPGQRIVVTGNPIGEVLAAMAER 179 Query: 181 IGASDILDRLNLMAGQYFLVTAHRAENVDDPESLRQIMSGLNLVAEHFGLRLICSIHPRT 240 I A+ L L L G Y L T HR ENVD P+ L +M+G+ V GL + S+HPRT Sbjct: 180 IDAATALGALGLRPGAYLLFTFHRQENVDVPQRLMALMAGVRRVMAVSGLPAVVSVHPRT 239 Query: 241 R-----SKLAEQFKLEMNPLVEFHEPFGFFDFVHLEQYALCAITDSGTVQEECCLMGVPT 295 R S LA+Q + ++ EPFGF DFV LE+ A C ++DSGTVQEE ++GVPT Sbjct: 240 RAHLEASGLADQGRGDLM----LMEPFGFCDFVALEKSARCVLSDSGTVQEEGAILGVPT 295 Query: 296 VTIRRTTERPETVDCGSNVVSGVEQESILRCTRLMTSLSPEWEAPEGYLTPDVSVKVVKF 355 VT+R TERPET++ GSN++ GV+ E++ R RL+T+ P W P Y +V+ VV+ Sbjct: 296 VTLRDVTERPETLETGSNILCGVDPEAMERAFRLVTATPPNWPVPLDYTRMNVADTVVRL 355 Query: 356 LLGGNLHV 363 L+G +L V Sbjct: 356 LIGASLPV 363 >gb|AAC46090.1| Cap5G [Staphylococcus aureus] Length = 374 Score = 328 bits (840), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 160/360 (44%), Positives = 243/360 (67%), Gaps = 7/360 (1%) Query: 1 MKIMTILGTRPEIIRLSVIIPLLDEHAEQHVLVHTGQNFTASLSGIFFAELGLRAPDYVL 60 +K+MTI+GTRPEIIRLS I D++ Q +LVHTGQN+ +L+ IFF +L LR PD+ L Sbjct: 4 LKLMTIVGTRPEIIRLSSTIKACDQYFNQ-ILVHTGQNYDYTLNQIFFDDLELRQPDHYL 62 Query: 61 QDKQAGLGGQLAAMFGSLESILLQEQPDRILLLGDTNSALCAILAERMGIPVVHMEAGNR 120 + + LG + + +LL+EQPD +L+LGDTNS L A+ A+R+ IPV HMEAGNR Sbjct: 63 EAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCLAAVSAKRLKIPVFHMEAGNR 122 Query: 121 CYDLKVPEEKNRRVIDAVSTINMPYTQQSKRHLLSEGFPSQRIVLTGNPIHEVIKHYNRQ 180 C+D VPEE NR+++D VS +N+PYT+ S+R+LL EGF I +TG+P+ EVI+ + + Sbjct: 123 CFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGFNKANIFVTGSPMTEVIEAHRDK 182 Query: 181 IGASDILDRLNLMAGQYFLVTAHRAENVDDPESLRQIMSGLNLVAEHFGLRLICSIHPRT 240 I SD+L++L L + QY LV+AHR EN+D+ ++ + +M+ +N +A+ + + +I S HPR+ Sbjct: 183 INHSDVLNKLGLESQQYILVSAHREENIDNEKNFKSLMNAINDIAKKYKMPVIYSTHPRS 242 Query: 241 RSKLAEQFKLEMNPLVEFHEPFGFFDFVHLEQYALCAITDSGTVQEECCLMGVPTVTIRR 300 K+ E+ K E +PLV+ +PFGFFD+ L++ A ++DSGT+ EE ++ P V IR Sbjct: 243 WKKI-EESKFEFDPLVKQLKPFGFFDYNALQKDAFVVLSDSGTLSEESSILKFPGVLIRT 301 Query: 301 TTERPETVDCGSNVVSGVEQESILRCTRLMTSLS----PEWEAPEGYLTPDVSVKVVKFL 356 +TERPE +D G+ +V G+ ++++ L + P +A + Y +VS KVVK + Sbjct: 302 STERPEVLDKGTVIVGGITYNNLIQSVELAREMQNNNEPMIDAID-YKDTNVSTKVVKII 360 >ref|NP_644945.1| capsular polysaccharide synthesis enzyme Cap8G [Staphylococcus aureus subsp. aureus MW2] dbj|BAB93995.1| capsular polysaccharide synthesis enzyme Cap8G [Staphylococcus aureus subsp. aureus MW2] Length = 374 Score = 327 bits (837), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 160/360 (44%), Positives = 242/360 (67%), Gaps = 7/360 (1%) Query: 1 MKIMTILGTRPEIIRLSVIIPLLDEHAEQHVLVHTGQNFTASLSGIFFAELGLRAPDYVL 60 +K+MTI+GTRPEIIRLS I D++ Q +LVHTGQN+ +L+ IFF +L LR PD+ L Sbjct: 4 LKLMTIVGTRPEIIRLSSTIKACDQYFNQ-ILVHTGQNYDYTLNQIFFDDLELRQPDHYL 62 Query: 61 QDKQAGLGGQLAAMFGSLESILLQEQPDRILLLGDTNSALCAILAERMGIPVVHMEAGNR 120 + + LG + + +LL+EQPD +L+LGDTNS L A+ A+R+ IPV HMEAGNR Sbjct: 63 EAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCLAAVSAKRLKIPVFHMEAGNR 122 Query: 121 CYDLKVPEEKNRRVIDAVSTINMPYTQQSKRHLLSEGFPSQRIVLTGNPIHEVIKHYNRQ 180 C+D VPEE NR+++D VS +N+PYT+ S+R+LL EGF I +TG+P+ EVI+ + + Sbjct: 123 CFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGFNKANIFVTGSPMTEVIEAHRDK 182 Query: 181 IGASDILDRLNLMAGQYFLVTAHRAENVDDPESLRQIMSGLNLVAEHFGLRLICSIHPRT 240 I SD+L++L L QY LV+AHR EN+D+ ++ + +M+ +N +A+ + + +I S HPR+ Sbjct: 183 INHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKSLMNAINDIAKKYKMPVIYSTHPRS 242 Query: 241 RSKLAEQFKLEMNPLVEFHEPFGFFDFVHLEQYALCAITDSGTVQEECCLMGVPTVTIRR 300 K+ E+ K E +PLV+ +PFGFFD+ L++ A ++DSGT+ EE ++ P V IR Sbjct: 243 WKKI-EESKFEFDPLVKKLKPFGFFDYNALQKDAFVVLSDSGTLSEESSILKFPGVLIRT 301 Query: 301 TTERPETVDCGSNVVSGVEQESILRCTRLMTSL----SPEWEAPEGYLTPDVSVKVVKFL 356 +TERPE +D G+ +V G+ ++++ L + P +A + Y +VS KVVK + Sbjct: 302 STERPEVLDKGTVIVGGITYNNLIQSVELAREMQNNNEPMIDAID-YKDTNVSTKVVKII 360 >ref|NP_370679.1| capsular polysaccharide synthesis enzyme Cap5G [Staphylococcus aureus subsp. aureus Mu50] ref|NP_373392.1| capsular polysaccharide synthesis enzyme Cap5G [Staphylococcus aureus subsp. aureus N315] pir||G89776 capsular polysaccharide synthesis enzyme Cap5G [imported] - Staphylococcus aureus (strain N315) gb|AAB49436.1| Cap8G [Staphylococcus aureus] dbj|BAB41370.1| capsular polysaccharide synthesis enzyme Cap5G [Staphylococcus aureus subsp. aureus N315] dbj|BAB56317.1| capsular polysaccharide synthesis enzyme Cap5G [Staphylococcus aureus subsp. aureus Mu50] Length = 374 Score = 326 bits (836), Expect = 5e-88, Method: Compositional matrix adjust. Identities = 160/360 (44%), Positives = 242/360 (67%), Gaps = 7/360 (1%) Query: 1 MKIMTILGTRPEIIRLSVIIPLLDEHAEQHVLVHTGQNFTASLSGIFFAELGLRAPDYVL 60 +K+MTI+GTRPEIIRLS I D++ Q +LVHTGQN+ +L+ IFF +L LR PD+ L Sbjct: 4 LKLMTIVGTRPEIIRLSSTIKACDQYFNQ-ILVHTGQNYDYTLNQIFFDDLELRQPDHYL 62 Query: 61 QDKQAGLGGQLAAMFGSLESILLQEQPDRILLLGDTNSALCAILAERMGIPVVHMEAGNR 120 + + LG + + +LL+EQPD +L+LGDTNS L A+ A+R+ IPV HMEAGNR Sbjct: 63 EAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCLAAVSAKRLKIPVFHMEAGNR 122 Query: 121 CYDLKVPEEKNRRVIDAVSTINMPYTQQSKRHLLSEGFPSQRIVLTGNPIHEVIKHYNRQ 180 C+D VPEE NR+++D VS +N+PYT+ S+R+LL EGF I +TG+P+ EVI+ + + Sbjct: 123 CFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGFNKANIFVTGSPMTEVIEAHRDK 182 Query: 181 IGASDILDRLNLMAGQYFLVTAHRAENVDDPESLRQIMSGLNLVAEHFGLRLICSIHPRT 240 I SD+L++L L QY LV+AHR EN+D+ ++ + +M+ +N +A+ + + +I S HPR+ Sbjct: 183 INHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKSLMNAINDIAKKYKMPVIYSTHPRS 242 Query: 241 RSKLAEQFKLEMNPLVEFHEPFGFFDFVHLEQYALCAITDSGTVQEECCLMGVPTVTIRR 300 K+ E+ K E +PLV+ +PFGFFD+ L++ A ++DSGT+ EE ++ P V IR Sbjct: 243 WKKI-EESKFEFDPLVKQLKPFGFFDYNALQKDAFVVLSDSGTLSEESSILKFPGVLIRT 301 Query: 301 TTERPETVDCGSNVVSGVEQESILRCTRLMTSL----SPEWEAPEGYLTPDVSVKVVKFL 356 +TERPE +D G+ +V G+ ++++ L + P +A + Y +VS KVVK + Sbjct: 302 STERPEVLDKGTVIVGGITYNNLIQSVELAREMQNNNEPMIDAID-YKDTNVSTKVVKII 360 >gb|AAD12956.1| unknown [Leptospira borgpetersenii] Length = 375 Score = 324 bits (831), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 154/357 (43%), Positives = 235/357 (65%), Gaps = 5/357 (1%) Query: 1 MKIMTILGTRPEIIRLSVIIPLLDEHAEQHVLVHTGQNFTASLSGIFFAELGLRAPDYVL 60 K+MTI+GTRPE I++S +I LD + +H+LVH+GQN+ L+ +FF +L +R PD+ L Sbjct: 2 FKVMTIVGTRPEFIKMSRVIAELDRNF-KHILVHSGQNYDYELNQVFFEDLEIRKPDHFL 60 Query: 61 QDKQAGLGGQLAAMFGSLESILLQEQPDRILLLGDTNSALCAILAERMGIPVVHMEAGNR 120 +A + + + +E+PD +LL GDTN+ L I A+R IP+ HMEAGNR Sbjct: 61 NAAGESAAATIAQVLLKADEVFEKEKPDALLLYGDTNTCLAVISAKRRKIPIFHMEAGNR 120 Query: 121 CYDLKVPEEKNRRVIDAVSTINMPYTQQSKRHLLSEGFPSQRIVLTGNPIHEVIKHYNRQ 180 C+D +VPEE NR+V+D +S IN+ T+ ++R+LL+EG + I+ TG+ + EV+K+Y + Sbjct: 121 CFDQRVPEELNRKVVDHLSDINLVLTEHARRYLLAEGIKPETIIKTGSHMDEVLKYYKVK 180 Query: 181 IGASDILDRLNLMAGQYFLVTAHRAENVDDPESLRQIMSGLNLVAEHFGLRLICSIHPRT 240 I SDIL++L L ++F+V+ HR ENVD E+L++++ LN + E +GL +I S HPRT Sbjct: 181 IEKSDILEKLKLEKNKFFIVSFHREENVDTIENLKKLLESLNAICEEYGLPVIVSTHPRT 240 Query: 241 RSKLAEQFKLEMNPLVEFHEPFGFFDFVHLEQYALCAITDSGTVQEECCLMGVPTVTIRR 300 R +L + ++EMNPL+ F +PFGFFD+V L+ A C ++DSGT+ EE L+ +P VTIR Sbjct: 241 RKRLEDFKEVEMNPLITFLKPFGFFDYVQLQMSAFCILSDSGTITEEASLLDLPAVTIRN 300 Query: 301 TTERPETVDCGSNVVSGVEQESILRCTRLMTSLSPE----WEAPEGYLTPDVSVKVV 353 T ERPE +D G+ ++SG +E IL R++ + + + Y VS K+V Sbjct: 301 THERPEGMDFGTLIMSGFSKERILESVRIVVDQYRDGIRHYRVVDDYSAGQVSKKIV 357 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.138 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 529,295,415 Number of extensions: 21306071 Number of successful extensions: 47822 Number of sequences better than 1.0e-05: 374 Number of HSP's gapped: 46975 Number of HSP's successfully gapped: 374 Length of query: 364 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 237 Effective length of database: 412,420,005 Effective search space: 97743541185 Effective search space used: 97743541185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)