BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0013.p (328 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_833475.1| UDP-N-acetylglucosamine 4,6-dehydratase [Bacill... 422 e-117 ref|ZP_00060852.1| COG1086: Predicted nucleoside-diphosphate sug... 268 1e-70 ref|ZP_00109039.1| COG1086: Predicted nucleoside-diphosphate sug... 256 4e-67 gb|EAK58637.1| unknown [environmental sequence] 255 8e-67 ref|NP_348811.1| Predicted nucleoside-diphosphate sugar epimeras... 254 2e-66 >ref|NP_833475.1| UDP-N-acetylglucosamine 4,6-dehydratase [Bacillus cereus ATCC 14579] gb|AAP10676.1| UDP-N-acetylglucosamine 4,6-dehydratase [Bacillus cereus ATCC 14579] Length = 328 Score = 422 bits (1085), Expect = e-117, Method: Compositional matrix adjust. Identities = 199/326 (61%), Positives = 253/326 (77%), Gaps = 1/326 (0%) Query: 4 NKRILVTGGTGSWGHELIRQLLPQNPKEIVIFSRSESAQVAMSREY-EDSRLSFIIGDIR 62 NK IL+TGGTGSWGHELI+QLL ++PKEI +FSR+E+ Q M +++ D RL FIIGDIR Sbjct: 3 NKIILITGGTGSWGHELIKQLLEKSPKEIRVFSRNETVQFEMQQQFINDHRLKFIIGDIR 62 Query: 63 DKDALVAACRGIDYVFHLAALKHVPVCEDQPYEALKTNVVGTQNVIEAAIACNVEKAIYI 122 DKD LV AC+G+ YVFHLAALKHVPVCE PYEA+KTN+ GTQNVIEA+ VEK IY+ Sbjct: 63 DKDQLVYACQGVHYVFHLAALKHVPVCEYYPYEAIKTNIHGTQNVIEASTQMQVEKVIYV 122 Query: 123 STDKAANPSNFYGMTKAIGEKLFVYANLLGSNTRFVTVRGGNVLGTNGSVVHLFMKQIKD 182 STDKAA+PSN YGMTKAIGEKL V+AN+ T+F+ VRGGNVLGT+GSVV LF +QIK Sbjct: 123 STDKAADPSNTYGMTKAIGEKLMVHANIQTKKTKFICVRGGNVLGTSGSVVPLFKQQIKR 182 Query: 183 KGQVRITDMKMTRFFFTLRDAITLLFKASEVSLGGEIFVMTMPTCRIVDLAEVLIEASGR 242 +V ITD MTRFF T+ DA+ LLFKA GGEIFVM MP C+I DLAE+LIE S + Sbjct: 183 SSEVGITDANMTRFFLTVEDAVGLLFKAVYQGRGGEIFVMKMPACKITDLAEILIEDSKK 242 Query: 243 RDVSIIEAGIRPGEKIHEILMSDFESQTTVVYDEQYLVILPTLDMPELKARYSKYPHVSF 302 ++ + E GIRPGEK+ E+L+S+ ES+T++ +D+ Y V+LPT+ + L+ YS YP V Sbjct: 243 ENIKVKEVGIRPGEKLSEMLLSEVESKTSISFDQNYFVVLPTIPIEGLQEYYSSYPLVDV 302 Query: 303 NSFSSENNLMDQREIKDILIRGGFLQ 328 SFSS+ +L+ + E+K +L++GGFL+ Sbjct: 303 KSFSSQQDLLAKHEVKQMLLKGGFLR 328 >ref|ZP_00060852.1| COG1086: Predicted nucleoside-diphosphate sugar epimerases [Clostridium thermocellum ATCC 27405] Length = 332 Score = 268 bits (685), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 141/332 (42%), Positives = 216/332 (65%), Gaps = 13/332 (3%) Query: 1 MFNNKRILVTGGTGSWGHELIRQLLPQNPKEIVIFSRSESAQVAMSREY-EDSRLSFIIG 59 MF +K+ILV GGTGS G LI ++L +NP I ++SR E Q ++ ++ E++ + ++IG Sbjct: 1 MFKDKKILVIGGTGSIGQALIERILTENPAVIRVYSRDEYKQFLLAEKFRENANIRYLIG 60 Query: 60 DIRDKDALVAACRGIDYVFHLAALKHVPVCEDQPYEALKTNVVGTQNVIEAAIACNVEKA 119 D+RD+D L A ID VF+LAALKHVP CE P+EA++TNV+G+QNVI A+A V+K Sbjct: 61 DVRDEDRLDRAMNDIDIVFNLAALKHVPACEYNPFEAVRTNVIGSQNVIACAMANKVKKV 120 Query: 120 IYISTDKAANPSNFYGMTKAIGEKLFVYANLLGSNTR--FVTVRGGNVLGTNGSVVHLFM 177 IY S+DKA +P+N G TK + E+L +N NT F +VR GNV+GT GSV+ LF Sbjct: 121 IYTSSDKAVSPTNTMGATKLLAERLMSSSNYSRGNTGTVFASVRFGNVMGTRGSVIPLFK 180 Query: 178 KQIKDKGQVRITDMKMTRFFFTLRDAITLLFKASEVSLGGEIFVMTMPTCRIVDLAEVLI 237 +Q+ +KG + +T+ +MTRF +L A+ L KA ++ GGE+FV+ MP R+ DLAEV+I Sbjct: 181 QQVLEKGYITVTEPEMTRFMMSLSQAVELTIKACSIARGGEVFVLKMPVIRLKDLAEVII 240 Query: 238 EASGRRD------VSIIEAGIRPGEKIHEILMSDFESQTTVVYDEQYLVILPTLDMPELK 291 E +++ + I + G+RPGEK++E LMS+ ES V ++ Y+++ P + + Sbjct: 241 EDMCKKNNIDPEKIEIKKIGLRPGEKMYEELMSEDESTKAVELNDMYVIMPPFENSYYIN 300 Query: 292 ARYSKYPHVSFNSFSSENNLMDQREIKDILIR 323 AR + V N S + + + +I+++L+R Sbjct: 301 ARKA----VIGNYSSQKEKTLTKSQIRELLVR 328 >ref|ZP_00109039.1| COG1086: Predicted nucleoside-diphosphate sugar epimerases [Nostoc punctiforme] Length = 329 Score = 256 bits (655), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 138/290 (47%), Positives = 191/290 (65%), Gaps = 10/290 (3%) Query: 1 MFNNKRILVTGGTGSWGHELIRQLLPQ-NPKEIVIFSRSESAQVAMSREYEDSRLSFIIG 59 MFN K IL+TGGTGS+G + ++ +L + P++++++SR E Q M++E+ + + + IG Sbjct: 1 MFNGKSILITGGTGSFGKQFVKTVLSRYQPQKVIVYSRDELKQYEMAQEFNEPVMRYFIG 60 Query: 60 DIRDKDALVAACRGIDYVFHLAALKHVPVCEDQPYEALKTNVVGTQNVIEAAIACNVEKA 119 D+RD+D L A RG+DYV H AALKH+P E P E +KTN+ G NVI+ A+ VEK Sbjct: 61 DVRDRDRLRLAMRGVDYVIHAAALKHIPAAEYNPMECIKTNIHGANNVIDIALDQEVEKV 120 Query: 120 IYISTDKAANPSNFYGMTKAIGEKLFVYA-NLLGS-NTRFVTVRGGNVLGTNGSVVHLFM 177 I +STDKA NP N YG TK +KLFV A N++G TRF VR GNV+G+ GSVV F Sbjct: 121 IALSTDKAVNPINLYGATKLAADKLFVAANNIVGEMKTRFAVVRYGNVVGSRGSVVPFFE 180 Query: 178 KQIKDKG-QVRITDMKMTRFFFTLRDAITLLFKASEVSLGGEIFVMTMPTCRIVDLAEVL 236 K I++K ++ ITD +MTRF+ TL+ A+ L+F + E GGEIFV +P+ +I DLAE L Sbjct: 181 KLIQEKSPEIPITDPRMTRFWITLQQAVDLVFNSFERMHGGEIFVPKIPSMKITDLAETL 240 Query: 237 IEASGRRDVSIIEAGIRPGEKIHEILMSDFESQTTVVYDEQYLVILPTLD 286 A G V I GIRPGEK+HE + S S + + + Y VI PT++ Sbjct: 241 --APG---VPIKIVGIRPGEKLHEAMFSSEASHLVLEFSDHY-VIRPTIE 284 >gb|EAK58637.1| unknown [environmental sequence] Length = 333 Score = 255 bits (652), Expect = 8e-67, Method: Compositional matrix adjust. Identities = 141/290 (48%), Positives = 185/290 (63%), Gaps = 9/290 (3%) Query: 1 MFNNKRILVTGGTGSWGHELIRQLLP-QNPKEIVIFSRSESAQVAMSREYEDSRLSFIIG 59 MF NK IL+TGGTGS+G I+ L+ N K+I+IFSR E+ Q M++ + S + F +G Sbjct: 1 MFKNKTILITGGTGSFGQACIKFLVKNHNLKKIIIFSRDENKQYEMNKRFPQSNIRFFLG 60 Query: 60 DIRDKDALVAACRGIDYVFHLAALKHVPVCEDQPYEALKTNVVGTQNVIEAAIACNVEKA 119 DIRD++ L A + +DYV H AALKHVP+ E P E +KTNV G QN+I AAI VEK Sbjct: 61 DIRDRERLTMAMKDVDYVIHAAALKHVPIGEYNPIEFIKTNVYGAQNIITAAIDAKVEKV 120 Query: 120 IYISTDKAANPSNFYGMTKAIGEKLFVYA-NLLGS-NTRFVTVRGGNVLGTNGSVVHLFM 177 + +STDKA NP N YG +K EKLF+ A N++G +T+F VR GNVL + GS+V LF+ Sbjct: 121 LALSTDKATNPINLYGASKLAAEKLFIAANNMVGKQHTKFSVVRYGNVLSSRGSIVPLFI 180 Query: 178 KQIKDKGQV-RITDMKMTRFFFTLRDAITLLFKASEVSLGGEIFVMTMPTCRIVDLAEVL 236 IK K + +TD KMTRFF L DAI + + GEIFV MP+ +I DLA+ L Sbjct: 181 NLIKQKNNIFPLTDEKMTRFFINLPDAIKFVLSCLQNMTRGEIFVPKMPSFKISDLAKTL 240 Query: 237 IEASGRRDVSIIEAGIRPGEKIHEILMSDFESQTTVVYDEQYLVILPTLD 286 + + II GIRPGEK+HEIL+SD ES+ T Y++ LD Sbjct: 241 ---NSKVKFKII--GIRPGEKLHEILISDNESRDTFESKNYYIIASKILD 285 >ref|NP_348811.1| Predicted nucleoside-diphosphate sugar epimerase [Clostridium acetobutylicum] pir||D97170 probable nucleoside-diphosphate sugar epimerase [imported] - Clostridium acetobutylicum gb|AAK80151.1| Predicted nucleoside-diphosphate sugar epimerase [Clostridium acetobutylicum] Length = 340 Score = 254 bits (648), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 146/339 (43%), Positives = 209/339 (61%), Gaps = 15/339 (4%) Query: 2 FNNKRILVTGGTGSWGHELIRQLLPQNPKEIVIFSRSESAQVAMSREYED--SRLSFIIG 59 F K +L+ GGTG+ GH LI +LL +NPK I IFSR E Q M E+ + F+IG Sbjct: 4 FTGKNVLIVGGTGTIGHGLINELLKENPKVIRIFSRDEYKQFLMQNEFRGIAEKFRFLIG 63 Query: 60 DIRDKDALVAACRGIDYVFHLAALKHVPVCEDQPYEALKTNVVGTQNVIEAAIACNVEKA 119 D+RD D + A GID VF+LAA+KHVP CE P EA++TN+ G +NVI+AA NVE Sbjct: 64 DVRDYDRVERAMNGIDVVFNLAAMKHVPACEYNPEEAIRTNITGMENVIKAATYHNVECV 123 Query: 120 IYISTDKAANPSNFYGMTKAIGEKLFVYANLLGSN--TRFVTVRGGNVLGTNGSVVHLFM 177 ++ S+DKA NP+N YG TK + EKL AN N T+FV VR GNV+G+ GSV+ LF Sbjct: 124 VFTSSDKAINPTNSYGATKLLAEKLVQAANYSKGNVRTKFVAVRFGNVMGSRGSVIPLFK 183 Query: 178 KQIKDKGQVRITDMKMTRFFFTLRDAITLLFKASEVSLGGEIFVMTMPTCRIVDLAEVLI 237 KQI++ + +TD M+RF TL A+ L+ A++ + GGE+F++ MP +++DLA+V++ Sbjct: 184 KQIEENAVITVTDPTMSRFMMTLSQAVKLIMTAAKEATGGEVFILKMPVIKLIDLAKVVV 243 Query: 238 EASGRR------DVSIIEAGIRPGEKIHEILMSDFESQTTVVYDEQYLVILPTLDMPELK 291 E + R+ + I E G+R GE+ +E LM+ ES Y+VI + + + K Sbjct: 244 EETSRKLDINPERIKIKEIGLRAGERCYEELMTREESVNAYDLGHSYVVIPSSFYITDYK 303 Query: 292 ARYSKYPHV---SFNSFSSENNLMDQREIKDILIRGGFL 327 ++YSKY S+NS S E +D E++ +L G L Sbjct: 304 SKYSKYKKAEIGSYNSSSIEPITLD--EVRQLLKNCGLL 340 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.137 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 461,360,303 Number of extensions: 17953339 Number of successful extensions: 57281 Number of sequences better than 1.0e-05: 1449 Number of HSP's gapped: 56775 Number of HSP's successfully gapped: 1449 Length of query: 328 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 202 Effective length of database: 415,135,104 Effective search space: 83857291008 Effective search space used: 83857291008 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)