BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0014.p (387 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_694007.1| N-acetylmuramoyl-L-alanine amidase [Oceanobacil... 201 3e-50 ref|NP_470892.1| similar to N-acetylmuramoyl-L-alanine amidase [... 194 2e-48 ref|NP_465046.1| similar to N-acetylmuramoyl-L-alanine amidase [... 192 1e-47 ref|NP_390636.1| similar to N-acetylmuramoyl-L-alanine amidase [... 160 5e-38 ref|NP_785516.1| N-acetylmuramoyl-L-alanine amidase [Lactobacill... 157 6e-37 >ref|NP_694007.1| N-acetylmuramoyl-L-alanine amidase [Oceanobacillus iheyensis HTE831] dbj|BAC15041.1| N-acetylmuramoyl-L-alanine amidase [Oceanobacillus iheyensis HTE831] Length = 379 Score = 201 bits (511), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 132/394 (33%), Positives = 188/394 (47%), Gaps = 24/394 (6%) Query: 1 MKKTLSTAMLAVTLLL---SNPGHPESAHAAGAYTAKVYASSLNVRSEPAASASVTGTLK 57 MK+ S A L +L+ S P HA G T +V +S+LN+RS P+ +S+ G Sbjct: 1 MKRKTSIATLFSMILIVMFSYLFSPAITHANGTDTYEVSSSTLNIRSAPSNESSIVGQFV 60 Query: 58 SGALVTVTDEQHGWLKVRAGSVSGWVAGYYLKRTXXXXXXXXXXXXXXXXXXXXXXXXXX 117 G +T EQ+ W++ G WVA ++L Sbjct: 61 KGNQLTTFKEQYDWVQTYYGGKEVWVAKHHL-------------VPVSTSNVSSTTSEMK 107 Query: 118 XXXTVQVTASSLRIRGGPGTGYKVLGSLKEGDSVTVLLRQGDWARIRTTGGEVGWVAGQY 177 +V V A S+ IR G GT YK S +GD V+ QGDW +++ GE GW+A Sbjct: 108 ENISVTVAAESVMIRAGAGTNYKNTHSAYQGDEFDVISSQGDWYQVKLPNGETGWIASWL 167 Query: 178 IARGAXXXXXXXXXXXXXXXXXXXXXXXXXXIRGKL----IVIDPGHGGSDPGMLGTSYD 233 G L IVIDPGHGG DPG +G D Sbjct: 168 TTEVGGVVSESTASDSSNSESVTNAPEQQPASSGSLAGYTIVIDPGHGGKDPGSIGLG-D 226 Query: 234 TMEKDLTLQTAFYVRDYLRAKGARVELTRTRGNQKPSLSRRVQIGQQLRADAFVSIHYNS 293 EKD+ T+ +V + L+ GA V TR+ G+ SL R +I + DAF+SIHY+S Sbjct: 227 VWEKDIVYSTSNHVVNQLKEAGANVITTRS-GDYFVSLEERARISNDYQTDAFISIHYDS 285 Query: 294 SP-KKVSGTLTFFYSENNDLRLARSIENRLRQGIGLKSNGLSFGDYHILRENSLPATLVE 352 P VSGT T +Y+ D LA I++ + + L + G +Y +LR+ + P+ L+E Sbjct: 286 FPLLSVSGTTT-YYAHKEDQPLAADIQSSIASSVELTNRGSMQANYKVLRDTTAPSVLLE 344 Query: 353 LGFLSNRSDESIVRTASYQKKAAKAIADGLADYF 386 LGF+SN+ D +IV+T YQ++ A AI +GL YF Sbjct: 345 LGFISNQYDVNIVQTDDYQRRVATAITNGLIQYF 378 >ref|NP_470892.1| similar to N-acetylmuramoyl-L-alanine amidase [Listeria innocua] pir||AC1627 N-acetylmuramoyl-L-alanine amidase homolog lin1556 [imported] - Listeria innocua (strain Clip11262) emb|CAC96787.1| lin1556 [Listeria innocua] Length = 427 Score = 194 bits (494), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 121/350 (34%), Positives = 179/350 (51%), Gaps = 36/350 (10%) Query: 40 LNVRSEPAASASVTGTLKSGALVTVTDEQHGWLKVRAGSVSGWVAGYYLKRTXXXXXXXX 99 LNVR +P+ S++ G L +G VTVT +Q+GW +++ S WV+ YL Sbjct: 109 LNVREKPSTSSTSLGLLNNGDQVTVTSQQNGWAQIQYNGKSAWVSSQYL----------- 157 Query: 100 XXXXXXXXXXXXXXXXXXXXXTVQVTASSLRIRGGPGTGYKVLGSLKEGDSVTVLLRQGD 159 TV + S IR PG V+ G + QGD Sbjct: 158 ------TIRESVTKVDESELQTVTIRDDSTNIRNKPGRDGAVIEKANSGQGFAIQGVQGD 211 Query: 160 WARIRTTGGEVGWVAGQYIARGAXXXXXXXXXXXXXXXXXXXXXXXXXXIRGKLIVIDPG 219 W +IRTT GE G+VA + + IVIDPG Sbjct: 212 WYKIRTTSGEEGYVANWVV-------------DVSDKGQTSSPRSKTTKLSEATIVIDPG 258 Query: 220 HGGSDPGMLGTSYDTMEKDLTLQTAFYVRDYLRAKGARVELTRTRGNQKPSLSRRVQIGQ 279 HGG+DPG G + T+EK++TL+TA +++ L ++GA+V LTR ++ SL R + Sbjct: 259 HGGNDPGAKGAN-GTIEKEMTLKTAKKLKEKLESRGAKVILTR-NSDKYVSLKSRTNVAA 316 Query: 280 QLRADAFVSIHYNS---SPKKVSGTLTFFYSENNDLRLARSIENRLRQGIGLKSNGLSFG 336 + +AD F+SIH++S S K VSG T++Y +N+D LA SI L + + G G Sbjct: 317 ENKADVFISIHFDSLEDSSKGVSGQTTYYY-DNSDKSLAESINTTLGNDLPTTNRGSRVG 375 Query: 337 DYHILRENSLPATLVELGFLSNRSDESIVRTASYQKKAAKAIADGLADYF 386 DY+++RENS PA L+ELG+LS+ DE + +ASY+ + A ++ DGL++YF Sbjct: 376 DYYVVRENSQPAVLLELGYLSSAKDERNINSASYRSQIADSVTDGLSNYF 425 >ref|NP_465046.1| similar to N-acetylmuramoyl-L-alanine amidase [Listeria monocytogenes EGD-e] pir||AI1264 N-acetylmuramoyl-L-alanine amidase homolog lmo1521 [imported] - Listeria monocytogenes (strain EGD-e) emb|CAC99599.1| lmo1521 [Listeria monocytogenes] Length = 427 Score = 192 bits (488), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 121/350 (34%), Positives = 178/350 (50%), Gaps = 36/350 (10%) Query: 40 LNVRSEPAASASVTGTLKSGALVTVTDEQHGWLKVRAGSVSGWVAGYYLKRTXXXXXXXX 99 LNVR +P+ S+ G L +G VTVT +Q+GW +++ S WV+ YL Sbjct: 109 LNVREKPSTSSKSLGLLNNGDQVTVTSQQNGWAQIQYNGTSAWVSSDYL----------- 157 Query: 100 XXXXXXXXXXXXXXXXXXXXXTVQVTASSLRIRGGPGTGYKVLGSLKEGDSVTVLLRQGD 159 TV + S IR P V+ G + QGD Sbjct: 158 ------TIRESVTKVDESELQTVTIRDDSTNIRNKPSRDGAVIEKANSGQGFAIQGVQGD 211 Query: 160 WARIRTTGGEVGWVAGQYIARGAXXXXXXXXXXXXXXXXXXXXXXXXXXIRGKLIVIDPG 219 W +IRTT GE G+VA + + IVIDPG Sbjct: 212 WYKIRTTSGEEGYVANWVV-------------DVSDKGQTSSPRSKTTKLSEATIVIDPG 258 Query: 220 HGGSDPGMLGTSYDTMEKDLTLQTAFYVRDYLRAKGARVELTRTRGNQKPSLSRRVQIGQ 279 HGG+DPG G + T+EK++TL+TA ++ L A+GA+V LTR ++ SL R I Sbjct: 259 HGGNDPGAKGAN-GTIEKEMTLKTAKQLKQKLEARGAKVILTR-NSDKYVSLKGRTNIAA 316 Query: 280 QLRADAFVSIHYNS---SPKKVSGTLTFFYSENNDLRLARSIENRLRQGIGLKSNGLSFG 336 + AD F+SIH++S + K VSG T++Y +N+D LA SI L + + + G G Sbjct: 317 ENNADVFISIHFDSLEDASKGVSGQTTYYY-DNSDKSLAESINTTLGKDLPTTNRGARVG 375 Query: 337 DYHILRENSLPATLVELGFLSNRSDESIVRTASYQKKAAKAIADGLADYF 386 DY+++RENS PA L+ELG+LS+ DE+ + +ASY+ + A+++ DGL++YF Sbjct: 376 DYYVVRENSQPAVLLELGYLSSAKDENNINSASYRSQIAESVTDGLSNYF 425 >ref|NP_390636.1| similar to N-acetylmuramoyl-L-alanine amidase [Bacillus subtilis] pir||B69981 N-acetylmuramoyl-L-alanine amidase homolog yrvJ - Bacillus subtilis emb|CAB14717.1| yrvJ [Bacillus subtilis subsp. subtilis str. 168] Length = 518 Score = 160 bits (405), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 106/356 (29%), Positives = 161/356 (45%), Gaps = 22/356 (6%) Query: 32 TAKVYASSLNVRSEPAASASVTGTLKSGALVTVTDEQHGWLKVRAGSVSGWVAGYYLKRT 91 T V SSLNVR+ + A++ L G +TV +E++GW + + GWVA +YL Sbjct: 183 TGTVGVSSLNVRASASHDAAIITKLDRGTKLTVLNEKNGWAHIEVNGLKGWVASHYL--- 239 Query: 92 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXTVQVTASSLRIRGGPGTGYKVLGSLKEGDSV 151 + +R T ++ +GDS Sbjct: 240 ---------LTSSVPADDSANAGSSSSAKKAYIMYGGTNLRSDASTSASIVERAAKGDSY 290 Query: 152 TVLLRQGDWARIRTTGGEVGWVAGQYIARGAXXXXXXXXXXXXXXXXXXXXXXXXXXIRG 211 T+ +G W I+ G+ +VA + + Sbjct: 291 TITGSKGSWYEIKLDNGQTAYVANWVV-------QTSKSAEEAGEPPVSDSPSGNGSLNN 343 Query: 212 KLIVIDPGHGGSDPGMLGTSYDTMEKDLTLQTAFYVRDYLRAKGARVELTRTRGNQKPSL 271 K I++DPGHGG D G +G S EK+LT++TA + LR+ GA V +TR + + SL Sbjct: 344 KTIIVDPGHGGKDSGTIGYS-GKFEKNLTIKTAKLLASKLRSAGADVYVTR-QDDTFVSL 401 Query: 272 SRRVQIGQQLRADAFVSIHYNS-SPKKVSGTLTFFYSENNDLRLARSIENRLRQGIGLKS 330 RV ADAF+SIHY+S + G+ ++YS D LA + + + + + Sbjct: 402 QSRVSTSHYRNADAFISIHYDSYADTSTRGSTAYYYSPAKDQELASDVHSEVVKRSSIPD 461 Query: 331 NGLSFGDYHILRENSLPATLVELGFLSNRSDESIVRTASYQKKAAKAIADGLADYF 386 G+ FGDY++LREN PA L ELG++S+ +E+IV + SYQ+K I GL YF Sbjct: 462 RGVLFGDYYVLRENRQPAMLYELGYVSHPQEEAIVHSNSYQEKVTDGIESGLEKYF 517 >ref|NP_785516.1| N-acetylmuramoyl-L-alanine amidase [Lactobacillus plantarum WCFS1] emb|CAD64365.1| N-acetylmuramoyl-L-alanine amidase [Lactobacillus plantarum WCFS1] Length = 282 Score = 157 bits (396), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 91/268 (33%), Positives = 142/268 (52%), Gaps = 23/268 (8%) Query: 121 TVQVTASSLRIRGGPGTGYKVLGSLKEGDSVTVLLRQGDWARIRTTGGEVGWVAGQYIAR 180 T V +++ IR GPG Y + + +G V ++ R+ +W +R + GW+A + Sbjct: 33 TATVNIANVNIRSGPGMSYAIEDATSKGTKVHIMKRKNNWLYVRYADHKFGWIASWLVNE 92 Query: 181 GAXXXXXXXXXXXXXXXXXXXXXXXXXXIRGKLIVIDPGHGGSDPGMLGTSYDTMEKDLT 240 I IV+DPGHGGSD G L +S MEK T Sbjct: 93 ------------------HNSQLTKTTKISEATIVLDPGHGGSDSGAL-SSKGKMEKTYT 133 Query: 241 LQTAFYVRDYLRAKGARVELTRTRGNQKPSLSRRVQIGQQLRADAFVSIHYNSSPKK--V 298 L+ A V LRA GA V LTR ++ LS R I +L ADAF+S H++SSP+K Sbjct: 134 LRVAKAVAKKLRAAGAHVVLTR-DSDEYVGLSARPAIANKLHADAFISFHFDSSPEKNTA 192 Query: 299 SGTLTFFYSENNDLRLARSIENRLRQGIGLKSNGLSFGDYHILRENSLPATLVELGFLSN 358 SG T++Y ++ L LA+++ N + + + S G FGD+ ++R+NS P+ L+ELG++++ Sbjct: 193 SGITTYYYHKSTSLALAKALNNNVST-LPIPSKGTDFGDFLVIRDNSRPSVLMELGYIND 251 Query: 359 RSDESIVRTASYQKKAAKAIADGLADYF 386 +SD + + Y ++ A A+ GL+ YF Sbjct: 252 KSDFKTISSKKYPQEVAHAVYAGLSTYF 279 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.132 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 480,809,487 Number of extensions: 16690937 Number of successful extensions: 48364 Number of sequences better than 1.0e-05: 285 Number of HSP's gapped: 48083 Number of HSP's successfully gapped: 289 Length of query: 387 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 260 Effective length of database: 412,420,005 Effective search space: 107229201300 Effective search space used: 107229201300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)