BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0007.p (431 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_978276.1| transport protein, NRAMP family [Bacillus cereu... 547 e-154 ref|NP_831576.1| Manganese transport protein mntH [Bacillus cere... 545 e-154 ref|NP_388317.1| similar to hypothetical proteins [Bacillus subt... 539 e-152 ref|NP_655742.1| Nramp, Natural resistance-associated macrophage... 528 e-149 ref|NP_295432.1| integral membrane protein, NRAMP family [Deinoc... 376 e-103 >ref|NP_978276.1| transport protein, NRAMP family [Bacillus cereus ATCC 10987] gb|AAS40884.1| transport protein, NRAMP family [Bacillus cereus ATCC 10987] Length = 431 Score = 547 bits (1409), Expect = e-154, Method: Compositional matrix adjust. Identities = 278/430 (64%), Positives = 327/430 (76%) Query: 2 MEQNLAETPPMIHRQKNKLTAQAVLNGDVKGLKRLLPFLGPAFIASVAYLDPGNFATNIT 61 M +++++ + + +A L+G KGLKRLLPFLGPAFIASVAY+DPGNFATNI Sbjct: 1 MNKDISKDEHVPSQSTTVQSAHLALSGQTKGLKRLLPFLGPAFIASVAYIDPGNFATNIA 60 Query: 62 AGSKYGYLLLWVIFASNLMAVLIQSLSAKLGIATGKNLPEVARERFPKGVSIFLWIQSEL 121 AGS+YGYLLLWVI ASNLMAVLIQ+LSAKLGIATG+NLPE+ARE FPK VSI LWIQ EL Sbjct: 61 AGSQYGYLLLWVILASNLMAVLIQTLSAKLGIATGRNLPEIARENFPKPVSIGLWIQGEL 120 Query: 122 VIIATDLAEFIGAALGLYLLFGIPMLPAALITAVGSFAILELQRRGYRTLEAGIAGMVLI 181 VI+ATDLAEFIGAALGLYLLFGIPMLPAALITA GSF ILE QRRG+R LEA I GM+ I Sbjct: 121 VIMATDLAEFIGAALGLYLLFGIPMLPAALITAAGSFIILEFQRRGFRPLEAIITGMIFI 180 Query: 182 VVLAFTFQVIMAKPDAGAVAAGIFTPQFQGVDSILLAAGILGATVMPHAIYLHSSLTQSR 241 VV+AF QV AKP+ + +G+F P+FQGVDSILLAAGILGATVMPHAIYLHS+LTQ R Sbjct: 181 VVIAFGVQVFYAKPELSPLLSGLFIPKFQGVDSILLAAGILGATVMPHAIYLHSALTQRR 240 Query: 242 VVGIDDKEKKQIFKLEFIDILIAMLIXXXXXXXXXXXXXXLFFKNGLVVEDLDIAFEQFR 301 VVG +D++KK+IF+ EFIDI+IAM+I LFFKNGL VEDLD+AF QF Sbjct: 241 VVGTNDEQKKKIFRFEFIDIIIAMVIAGAINASMLIVAAALFFKNGLHVEDLDVAFNQFS 300 Query: 302 HLAGPVTAVSFXXXXXXXXXXXXXXXTMAGDVVMQGFINKRINLYLRRXXXXXXXXXXXX 361 L GPV+A F TM+GD++MQGFI I LYLRR Sbjct: 301 TLVGPVSAALFGIGLLSAGLSSSSVGTMSGDIIMQGFIRMHIPLYLRRFITMIPPLVIIA 360 Query: 362 FGINATSALVMSQVVLSFGIAFALIPLVIFTSDRKIMNGLVNHRITTVLGWIISALVVSL 421 G+N T ALVMSQVVLSFGIAFAL+PL++FTS++KIM LVNHRITT++ WII+ LV+ L Sbjct: 361 LGVNPTYALVMSQVVLSFGIAFALVPLIMFTSNKKIMGALVNHRITTLIAWIIAILVIIL 420 Query: 422 NLFLIVEMFV 431 N+FL+ + FV Sbjct: 421 NIFLLYQTFV 430 >ref|NP_831576.1| Manganese transport protein mntH [Bacillus cereus ATCC 14579] gb|AAP08777.1| Manganese transport protein mntH [Bacillus cereus ATCC 14579] Length = 431 Score = 545 bits (1404), Expect = e-154, Method: Compositional matrix adjust. Identities = 277/430 (64%), Positives = 324/430 (75%) Query: 2 MEQNLAETPPMIHRQKNKLTAQAVLNGDVKGLKRLLPFLGPAFIASVAYLDPGNFATNIT 61 M +++ + + +A L+G+ KG KRLLPFLGPAFIASVAY+DPGNFATNI Sbjct: 1 MNKDITKDEHLPSESTTVQSAHLALSGETKGWKRLLPFLGPAFIASVAYIDPGNFATNIA 60 Query: 62 AGSKYGYLLLWVIFASNLMAVLIQSLSAKLGIATGKNLPEVARERFPKGVSIFLWIQSEL 121 AGS+YGYLLLWVI ASNLMAVLIQ+LSAKLGIATG NLPE+ARE FPK VSI LWIQ EL Sbjct: 61 AGSQYGYLLLWVILASNLMAVLIQTLSAKLGIATGNNLPEIARENFPKPVSIGLWIQGEL 120 Query: 122 VIIATDLAEFIGAALGLYLLFGIPMLPAALITAVGSFAILELQRRGYRTLEAGIAGMVLI 181 VI+ATDLAEFIGAALGLYLLFGIPMLPAALITA GSF ILE QRRG+R LEA I GM+ I Sbjct: 121 VIMATDLAEFIGAALGLYLLFGIPMLPAALITAAGSFIILEFQRRGFRPLEAIITGMIFI 180 Query: 182 VVLAFTFQVIMAKPDAGAVAAGIFTPQFQGVDSILLAAGILGATVMPHAIYLHSSLTQSR 241 VV+AF QV AKP+ + +G+F P+FQGVDSILLAAGILGATVMPHAIYLHS+LTQ R Sbjct: 181 VVIAFGIQVFYAKPELSPLLSGLFIPKFQGVDSILLAAGILGATVMPHAIYLHSALTQRR 240 Query: 242 VVGIDDKEKKQIFKLEFIDILIAMLIXXXXXXXXXXXXXXLFFKNGLVVEDLDIAFEQFR 301 VVG D++KK+IF+ EFIDI+IAM+I LFFKNGL VEDLD+A+ QF Sbjct: 241 VVGTTDEQKKKIFRFEFIDIIIAMVIAGAINASMLIVAAALFFKNGLHVEDLDVAYTQFT 300 Query: 302 HLAGPVTAVSFXXXXXXXXXXXXXXXTMAGDVVMQGFINKRINLYLRRXXXXXXXXXXXX 361 +L GPV+A F TM+GD++MQGFI I LYLRR Sbjct: 301 NLVGPVSAALFGIGLLSAGLSSSSVGTMSGDIIMQGFIRMHIPLYLRRFITMIPPLVIIA 360 Query: 362 FGINATSALVMSQVVLSFGIAFALIPLVIFTSDRKIMNGLVNHRITTVLGWIISALVVSL 421 G+N T ALVMSQVVLSFGIAFAL+PL++FTS++KIM LVNHRITT + WII+ALV+ L Sbjct: 361 LGVNPTYALVMSQVVLSFGIAFALVPLIMFTSNKKIMGALVNHRITTFIAWIIAALVIVL 420 Query: 422 NLFLIVEMFV 431 N+FL+ + FV Sbjct: 421 NIFLLYQTFV 430 >ref|NP_388317.1| similar to hypothetical proteins [Bacillus subtilis] sp|P96593|MNTH_BACSU Manganese transport protein mntH pir||A69770 conserved hypothetical protein ydaR - Bacillus subtilis dbj|BAA19273.1| PROBABLE INTEGRAL MEMBRANE PROTEIN. [Bacillus subtilis] emb|CAB12243.1| manganese transporter [Bacillus subtilis subsp. subtilis str. 168] Length = 425 Score = 539 bits (1388), Expect = e-152, Method: Compositional matrix adjust. Identities = 273/430 (63%), Positives = 326/430 (75%), Gaps = 6/430 (1%) Query: 1 MMEQNLAETPPMIHRQKNKLTAQAVLNGDVKGLKRLLPFLGPAFIASVAYLDPGNFATNI 60 MM +++ P R K Q L+G ++G + LLPFLGPAFIA++AY+DPGNFATNI Sbjct: 1 MMNKDITAQSP---RSK---AVQDALDGKIRGFRGLLPFLGPAFIAAIAYIDPGNFATNI 54 Query: 61 TAGSKYGYLLLWVIFASNLMAVLIQSLSAKLGIATGKNLPEVARERFPKGVSIFLWIQSE 120 +AGSKYGY+LLWVI SN+MA+LIQSLSAKLGIATGKNLPEVARE FPK VSI LWIQ E Sbjct: 55 SAGSKYGYMLLWVILFSNIMALLIQSLSAKLGIATGKNLPEVAREEFPKPVSIGLWIQGE 114 Query: 121 LVIIATDLAEFIGAALGLYLLFGIPMLPAALITAVGSFAILELQRRGYRTLEAGIAGMVL 180 LVIIATDLAEFIGAALGLYLLFGIPML A++I A+GSFAILELQRRGYR+LEAGIAGM+ Sbjct: 115 LVIIATDLAEFIGAALGLYLLFGIPMLEASIIAAIGSFAILELQRRGYRSLEAGIAGMLF 174 Query: 181 IVVLAFTFQVIMAKPDAGAVAAGIFTPQFQGVDSILLAAGILGATVMPHAIYLHSSLTQS 240 +VV+AF Q AKPDA +V G+F P F G DS+LLAAGILGATVMPHAIYLHS+LTQ Sbjct: 175 VVVIAFALQTFFAKPDAVSVMKGLFVPAFHGTDSVLLAAGILGATVMPHAIYLHSALTQR 234 Query: 241 RVVGIDDKEKKQIFKLEFIDILIAMLIXXXXXXXXXXXXXXLFFKNGLVVEDLDIAFEQF 300 RVVG D E+K+IF+ EFIDILIAMLI LFFKNGL VEDLD+AF+QF Sbjct: 235 RVVGKTDAERKKIFRFEFIDILIAMLIAGAINASMLIVAAALFFKNGLFVEDLDVAFQQF 294 Query: 301 RHLAGPVTAVSFXXXXXXXXXXXXXXXTMAGDVVMQGFINKRINLYLRRXXXXXXXXXXX 360 HL P++A F T++GDV+MQGFIN RI LY+RR Sbjct: 295 GHLVSPMSAALFGIGLLVAGLSSSSVGTLSGDVIMQGFINYRIPLYVRRFITIIPPILII 354 Query: 361 XFGINATSALVMSQVVLSFGIAFALIPLVIFTSDRKIMNGLVNHRITTVLGWIISALVVS 420 G+N T+ALV+SQVVLSFGIAFALIPL++FTS+++IM L+N + TV+ W+I+ L+V+ Sbjct: 355 ASGVNPTTALVLSQVVLSFGIAFALIPLIMFTSNKRIMGSLINAKWITVVSWLIAVLIVA 414 Query: 421 LNLFLIVEMF 430 LN+FLIV+ F Sbjct: 415 LNVFLIVDTF 424 >ref|NP_655742.1| Nramp, Natural resistance-associated macrophage protein [Bacillus anthracis A2012] ref|NP_844295.1| transport protein, NRAMP family [Bacillus anthracis str. Ames] gb|AAP25781.1| transport protein, NRAMP family [Bacillus anthracis str. Ames] Length = 431 Score = 528 bits (1361), Expect = e-149, Method: Compositional matrix adjust. Identities = 277/430 (64%), Positives = 326/430 (75%) Query: 2 MEQNLAETPPMIHRQKNKLTAQAVLNGDVKGLKRLLPFLGPAFIASVAYLDPGNFATNIT 61 M +++++ + + +A L+G KGLKRLLPFLGPAFIASVAY+DPGNFATNI Sbjct: 1 MNKDISKDEHVPSQSTTVQSAHLALSGQTKGLKRLLPFLGPAFIASVAYIDPGNFATNIA 60 Query: 62 AGSKYGYLLLWVIFASNLMAVLIQSLSAKLGIATGKNLPEVARERFPKGVSIFLWIQSEL 121 AGS+YGYLLLWVI ASNLMAVLIQ+LSAKLGIATG+NLPE+ARE FPK VSI LWIQ EL Sbjct: 61 AGSQYGYLLLWVILASNLMAVLIQTLSAKLGIATGRNLPEIARENFPKPVSIGLWIQGEL 120 Query: 122 VIIATDLAEFIGAALGLYLLFGIPMLPAALITAVGSFAILELQRRGYRTLEAGIAGMVLI 181 VI+ATDLAEFIGAALGLYLLFGIPMLPAALITA GSF ILE QRRG+R LEA I GM+ I Sbjct: 121 VIMATDLAEFIGAALGLYLLFGIPMLPAALITAAGSFIILEFQRRGFRPLEAIITGMIFI 180 Query: 182 VVLAFTFQVIMAKPDAGAVAAGIFTPQFQGVDSILLAAGILGATVMPHAIYLHSSLTQSR 241 VV+AF QV AKP+ + +G+F P+FQGVDSILLAAGILGATVMPHAIYLHS+LTQ R Sbjct: 181 VVIAFGVQVFYAKPELSPLLSGLFIPKFQGVDSILLAAGILGATVMPHAIYLHSALTQRR 240 Query: 242 VVGIDDKEKKQIFKLEFIDILIAMLIXXXXXXXXXXXXXXLFFKNGLVVEDLDIAFEQFR 301 VVG +D++KK+IF+ +FIDI+IAM+I LFFKNGL VEDLD+AF QF Sbjct: 241 VVGTNDEQKKKIFRFDFIDIIIAMIIAGAINASMLIVAAALFFKNGLHVEDLDVAFNQFS 300 Query: 302 HLAGPVTAVSFXXXXXXXXXXXXXXXTMAGDVVMQGFINKRINLYLRRXXXXXXXXXXXX 361 +L GPV+A F TM+GD++MQGFI I LYLRR Sbjct: 301 NLVGPVSAALFGIGLLSAGLSSSSVGTMSGDIIMQGFIRMHIPLYLRRFITMIPPLVIIA 360 Query: 362 FGINATSALVMSQVVLSFGIAFALIPLVIFTSDRKIMNGLVNHRITTVLGWIISALVVSL 421 G+N T ALVMSQVVLSFGIAFAL+PL++FTS +KIM LVNHRITT + WII+ LV+ L Sbjct: 361 LGVNPTYALVMSQVVLSFGIAFALVPLIMFTSSKKIMGTLVNHRITTFIAWIIAVLVIIL 420 Query: 422 NLFLIVEMFV 431 N+FL+ + FV Sbjct: 421 NIFLLYQTFV 430 >ref|NP_295432.1| integral membrane protein, NRAMP family [Deinococcus radiodurans] sp|Q9RTP8|MNTH_DEIRA Probable manganese transport protein mntH pir||G75363 integral membrane protein, NRAMP family - Deinococcus radiodurans (strain R1) gb|AAF11265.1| integral membrane protein, NRAMP family [Deinococcus radiodurans] Length = 436 Score = 376 bits (965), Expect = e-103, Method: Compositional matrix adjust. Identities = 196/431 (45%), Positives = 275/431 (63%), Gaps = 4/431 (0%) Query: 3 EQNLAETPPMIHRQKNKLTAQAVLNGDV--KGLKRLLPFLGPAFIASVAYLDPGNFATNI 60 +L + P Q A A L G +G++R+LPFLGPA IAS+AY+DPGNFATNI Sbjct: 5 SPSLPDDRPDPPEQHLDARAGATLRGTAGPRGVRRILPFLGPAVIASIAYMDPGNFATNI 64 Query: 61 TAGSKYGYLLLWVIFASNLMAVLIQSLSAKLGIATGKNLPEVARERFPKGVSIFLWIQSE 120 G++YGY LLWVI A+NLMA++IQ+LSA LGIA+G+NLPE+ RER+P+ + F WIQ+E Sbjct: 65 EGGARYGYSLLWVILAANLMAMVIQNLSANLGIASGRNLPELIRERWPRPLVWFYWIQAE 124 Query: 121 LVIIATDLAEFIGAALGLYLLFGIPMLPAALITAVGSFAILELQRRGYRTLEAGIAGMVL 180 LV +ATDLAEF+GAAL + LL G+PM A++T V +F +L LQ+RG R LE + VL Sbjct: 125 LVAMATDLAEFLGAALAIQLLTGLPMFWGAVVTGVVTFWLLNLQKRGTRPLELAVGAFVL 184 Query: 181 IVVLAFTFQVIMAKPDAGAVAAGIFTPQFQGVDSILLAAGILGATVMPHAIYLHSSLTQS 240 ++ +A+ QV++A+PD AV AG F P+ QG S LA GI+GATVMPH IYLHS+LTQ Sbjct: 185 MIGVAYLVQVVLARPDLAAVGAG-FVPRLQGPGSAYLAVGIIGATVMPHVIYLHSALTQG 243 Query: 241 RVVGIDDKEKKQIFKLEFIDILIAMLIXXXXXXXXXXXXXXLFF-KNGLVVEDLDIAFEQ 299 R+ +EK+++ +L +D++ AM + F KN DL A++ Sbjct: 244 RIQTDTTEEKRRLVRLNRVDVIAAMGLAGLINMSMLAVAAATFHGKNVENAGDLTTAYQT 303 Query: 300 FRHLAGPVTAVSFXXXXXXXXXXXXXXXTMAGDVVMQGFINKRINLYLRRXXXXXXXXXX 359 L GP +V F TMAGDV+MQGF+ I L+LRR Sbjct: 304 LTPLLGPAASVLFAVALLASGLSSSAVGTMAGDVIMQGFMGFHIPLWLRRLITMLPAFIV 363 Query: 360 XXFGINATSALVMSQVVLSFGIAFALIPLVIFTSDRKIMNGLVNHRITTVLGWIISALVV 419 G++ +S L++SQV+L FG+ FAL+PL++FT+ R +M LV R TV+GW+I+ +++ Sbjct: 364 ILLGMDPSSVLILSQVILCFGVPFALVPLLLFTARRDVMGALVTRRSFTVIGWVIAVIII 423 Query: 420 SLNLFLIVEMF 430 +LN +L+ E+ Sbjct: 424 ALNGYLLWELL 434 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.328 0.142 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 502,210,561 Number of extensions: 17596129 Number of successful extensions: 63861 Number of sequences better than 1.0e-05: 270 Number of HSP's gapped: 63405 Number of HSP's successfully gapped: 271 Length of query: 431 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 303 Effective length of database: 409,704,906 Effective search space: 124140586518 Effective search space used: 124140586518 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 127 (53.5 bits)