BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0006.p (563 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_561561.1| glutamine-tRNA ligase [Clostridium perfringens]... 615 e-175 ref|NP_954406.1| glutaminyl-tRNA synthetase [Geobacter sulfurred... 606 e-172 ref|ZP_00081484.1| COG0008: Glutamyl- and glutaminyl-tRNA synthe... 602 e-170 ref|ZP_00124513.1| COG0008: Glutamyl- and glutaminyl-tRNA synthe... 601 e-170 ref|ZP_00097505.1| COG0008: Glutamyl- and glutaminyl-tRNA synthe... 598 e-169 >ref|NP_561561.1| glutamine-tRNA ligase [Clostridium perfringens] sp|Q8XMP3|SYQ_CLOPE Glutaminyl-tRNA synthetase (Glutamine--tRNA ligase) (GlnRS) dbj|BAB80351.1| glutamine-tRNA ligase [Clostridium perfringens str. 13] Length = 552 Score = 615 bits (1587), Expect = e-175, Method: Compositional matrix adjust. Identities = 303/549 (55%), Positives = 389/549 (70%), Gaps = 5/549 (0%) Query: 12 AENYMDKLIKEDIESGIYSREICTRFPPEPNGYLHIGSAFAIHTNYGLAHKYGGTFHLRF 71 + N++ +I +D++S + I TRFPPEPNGYLHIG A +I N+ L ++ G +LRF Sbjct: 7 SSNFIRNIIIDDLDSKKHDT-IITRFPPEPNGYLHIGHAKSIVLNFELGKEFNGRTNLRF 65 Query: 72 DDTNPLKEDMEYVKAIIQDIEWLGCSPGEHIYYGSDYSEQIYNSALNLIRKGKAYVCDLS 131 DDTNP KED EYV++I +D+ WLG + E +++ S+Y +++Y AL LI+KGKAYVCDL+ Sbjct: 66 DDTNPTKEDTEYVESIKEDVHWLGYNWSE-LHFASNYFDEMYKRALLLIKKGKAYVCDLT 124 Query: 132 PEEVTAYRGTLTEPGKNSPYRERSVEENLRLFSGMREGAYPTGSRVLRAKIDMSSPNINL 191 PEE+ YRGTLTEPGK SPYR RS+EENL LF MR+G + GS+VLRAKIDMSSPNIN Sbjct: 125 PEEIKEYRGTLTEPGKESPYRNRSIEENLDLFERMRKGEFEDGSKVLRAKIDMSSPNINF 184 Query: 192 RDPVIYRIIHAEHYRTGNAWCIYPMYDFAHPIQDAIEGITHSLCSLEFKDHRPLYEWVLE 251 RDP+IYRI HA H+ TG+ WCIYPMYDFAHP++DAIEGITHS+C+LEF DHRPLY+W ++ Sbjct: 185 RDPIIYRIAHASHHNTGDKWCIYPMYDFAHPLEDAIEGITHSICTLEFADHRPLYDWFVK 244 Query: 252 ELGIEEAPRQREFGRVNLTGVVTGKRYLRALVAGNYVDGWDDXXXXXXXXXXXXXXXXES 311 E +E PRQ EF R+N+T V KR L+ LV VDGWDD +S Sbjct: 245 ECEMESVPRQIEFARLNITNTVMSKRKLKQLVDEGIVDGWDDPRMPTVAGLRRRGYTPKS 304 Query: 312 IRSFVREIGMVRNQTMVDFSMLEHCLRQDLKASVPSIMAVLDPLKVVITNYEAGETEWLP 371 IR+F + IG+ + + VD MLEH +R+DL+ + P MAV++PLK+VITNY GE+E L Sbjct: 305 IRNFCKAIGVAKADSTVDSQMLEHFIREDLQETAPRAMAVINPLKLVITNYPEGESEILE 364 Query: 372 LANNSENAELGVREVPFSGTVYIERDDFMEEPAPGFRRLVPGGEVRLKGAYIIKCNEVIK 431 + NN ++ G R V FS VYIER+DFME P + RL PG EVRLKGAY +KCNEVIK Sbjct: 365 IENNPKDESAGKRAVTFSREVYIEREDFMENPPKKYFRLFPGNEVRLKGAYFVKCNEVIK 424 Query: 432 DAGTGAVRELRCTYDPLTKSGGANSGRKVKGTLHWVSASHGIPAEVYLYEKLLEDNEGPK 491 D G V E+ CTYDP TKSG +GRKVKGT+HWV A++ IPAE LYE L+ D + + Sbjct: 425 DEN-GEVTEIHCTYDPETKSGTGFTGRKVKGTIHWVDANNCIPAEFRLYEPLILD-DCEE 482 Query: 492 AEGETWEEYINPDSIRLMLSCLLEPFAAGAKPEDKFQFMRQGYFCVDNKLSREDRLVFNR 551 EG+ + E INP+S+ + +EP A AKP+DK+Q R GYF VD + +D LVFNR Sbjct: 483 NEGKHFLEQINPNSLTI-CKGFIEPSAKDAKPQDKYQLFRHGYFNVDPNFTSDDNLVFNR 541 Query: 552 IVPLKDTWK 560 IV LK ++K Sbjct: 542 IVSLKSSFK 550 >ref|NP_954406.1| glutaminyl-tRNA synthetase [Geobacter sulfurreducens PCA] gb|AAR36756.1| glutaminyl-tRNA synthetase [Geobacter sulfurreducens PCA] Length = 554 Score = 606 bits (1562), Expect = e-172, Method: Compositional matrix adjust. Identities = 298/555 (53%), Positives = 382/555 (68%), Gaps = 5/555 (0%) Query: 5 NAMETAAAENYMDKLIKEDIESGIYSREICTRFPPEPNGYLHIGSAFAIHTNYGLAHKYG 64 + E A N++ ++++D++SG + R I TRFPPEPNGYLHIG A +I N+GLA +G Sbjct: 2 STTEPAHVTNFLRTIVEDDLKSGKH-RTIVTRFPPEPNGYLHIGHAKSICLNFGLARDFG 60 Query: 65 GTFHLRFDDTNPLKEDMEYVKAIIQDIEWLGCSPGEHIYYGSDYSEQIYNSALNLIRKGK 124 G HLRFDDTNP+KE+ EY+++I + + WLG GEH YY SDY EQ+Y A +LI +GK Sbjct: 61 GRCHLRFDDTNPVKEETEYIESIKESVRWLGFEWGEHCYYASDYFEQLYQWAESLIIQGK 120 Query: 125 AYVCDLSPEEVTAYRGTLTEPGKNSPYRERSVEENLRLFSGMREGAYPTGSRVLRAKIDM 184 AYV DL+ +E+ +YRGTLTEPGK SP R R+ EENL LF MR G +P G++VLRA+IDM Sbjct: 121 AYVDDLTADEIRSYRGTLTEPGKESPSRSRTAEENLDLFRRMRAGEFPDGAKVLRARIDM 180 Query: 185 SSPNINLRDPVIYRIIHAEHYRTGNAWCIYPMYDFAHPIQDAIEGITHSLCSLEFKDHRP 244 +SPNINLRDPV+YRI+HA H G+ WCIYPMYD+AH DAIEGITHS+C+LEF+DH+P Sbjct: 181 ASPNINLRDPVMYRILHAPHPHAGDKWCIYPMYDYAHGQSDAIEGITHSICTLEFEDHKP 240 Query: 245 LYEWVLEELGIEEAPRQREFGRVNLTGVVTGKRYLRALVAGNYVDGWDDXXXXXXXXXXX 304 LYEW L+ L + PRQ EF R+NLT V KR L LV V GWDD Sbjct: 241 LYEWFLDNLPVPNRPRQYEFARLNLTYAVMSKRKLLQLVKDGDVTGWDDPRMPTIMGIRR 300 Query: 305 XXXXXESIRSFVREIGMVRNQTMVDFSMLEHCLRQDLKASVPSIMAVLDPLKVVITNYEA 364 E+IR+F IG+ R+ + +D S+LE +RQDL P +MAVL PL++VI NY Sbjct: 301 RGFTPEAIRNFCDTIGVGRSDSWIDMSILEESVRQDLNERAPRVMAVLRPLRLVIENYPE 360 Query: 365 GETEWLPLANNSENAELGVREVPFSGTVYIERDDFMEEPAPGFRRLVPGGEVRLKGAYII 424 G TE L +A + + +LG R VPF ++IERDDFME P GF+RL PG E+RL+GAYI+ Sbjct: 361 GATEDLTIAYHPQRPDLGSRSVPFGRELFIERDDFMEVPPKGFKRLSPGEEIRLRGAYIV 420 Query: 425 KCNEVIKDAGTGAVRELRCTYDPLTKSGGANSGRKVKGTLHWVSASHGIPAEVYLYEKLL 484 KC V +D G G V +RCTYDP T+SG S RKVKG +HWVSA+H + AEV LY++L Sbjct: 421 KCTGVERD-GEGNVTTVRCTYDPETRSGLPGSERKVKGVIHWVSATHAVTAEVRLYDRLF 479 Query: 485 EDNEGPKAEGETWEEYINPDSIRLMLSCLLEPFAAGAKPEDKFQFMRQGYFCVDNKLSRE 544 P G+ W+E +NP S+ ++ C LEP A A+PED+FQF RQGYFC D S+ Sbjct: 480 ---TVPNPSGDDWKELLNPLSVEIVRDCRLEPSLALARPEDRFQFERQGYFCADRVDSQP 536 Query: 545 DRLVFNRIVPLKDTW 559 VFNR V L+D+W Sbjct: 537 GAPVFNRTVTLRDSW 551 >ref|ZP_00081484.1| COG0008: Glutamyl- and glutaminyl-tRNA synthetases [Geobacter metallireducens] Length = 553 Score = 602 bits (1551), Expect = e-170, Method: Compositional matrix adjust. Identities = 301/555 (54%), Positives = 384/555 (69%), Gaps = 7/555 (1%) Query: 6 AMETAAAENYMDKLIKEDIESGIYSRE-ICTRFPPEPNGYLHIGSAFAIHTNYGLAHKYG 64 + ET N++ +++ED++ G RE I TRFPPEPNGYLHIG A +I N+GLA ++G Sbjct: 2 STETPHTANFLRTIVQEDLKGG--KRETIVTRFPPEPNGYLHIGHAKSICLNFGLAKEFG 59 Query: 65 GTFHLRFDDTNPLKEDMEYVKAIIQDIEWLGCSPGEHIYYGSDYSEQIYNSALNLIRKGK 124 G HLRFDDTNP+KE+ EYV++I + + WLG S G H+YY S+Y EQ+Y A +LI +GK Sbjct: 60 GRCHLRFDDTNPVKEETEYVESIKESVRWLGFSWGTHLYYASEYFEQLYQWAEHLILQGK 119 Query: 125 AYVCDLSPEEVTAYRGTLTEPGKNSPYRERSVEENLRLFSGMREGAYPTGSRVLRAKIDM 184 AYV DL+ EE+ A+RGTLTE GK SP+R RS EENL LF MR G +P G++VLRAKIDM Sbjct: 120 AYVDDLTAEEIRAHRGTLTEAGKESPWRNRSPEENLDLFRRMRAGEFPDGAKVLRAKIDM 179 Query: 185 SSPNINLRDPVIYRIIHAEHYRTGNAWCIYPMYDFAHPIQDAIEGITHSLCSLEFKDHRP 244 +SPN+NLRDPV+YRIIHA H G+AW IYPMYD+AH DAIEGITHS+C+LEF+DH+P Sbjct: 180 ASPNMNLRDPVMYRIIHASHPHVGDAWRIYPMYDYAHGQSDAIEGITHSICTLEFEDHKP 239 Query: 245 LYEWVLEELGIEEAPRQREFGRVNLTGVVTGKRYLRALVAGNYVDGWDDXXXXXXXXXXX 304 LYEW L+ L + PRQ EF R+NLT V KR L LV V GWDD Sbjct: 240 LYEWFLDNLPVPNRPRQYEFARLNLTYAVMSKRKLLQLVKDGDVSGWDDPRMPTIMGIRR 299 Query: 305 XXXXXESIRSFVREIGMVRNQTMVDFSMLEHCLRQDLKASVPSIMAVLDPLKVVITNYEA 364 E++R+F IG+ R+ + +D S+LE +RQDL P +MAVL PL++VI +Y Sbjct: 300 RGYTPEAVRNFCETIGVGRSDSWIDMSILEESVRQDLNERAPRVMAVLKPLRLVIEDYPE 359 Query: 365 GETEWLPLANNSENAELGVREVPFSGTVYIERDDFMEEPAPGFRRLVPGGEVRLKGAYII 424 GE E L + + + ELG R VPF ++IE+DDF E P GF+RL PG E+RL+GAYII Sbjct: 360 GEAEELTIPYHPQKPELGSRVVPFCSELFIEQDDFAEVPPKGFKRLSPGEEIRLRGAYII 419 Query: 425 KCNEVIKDAGTGAVRELRCTYDPLTKSGGANSGRKVKGTLHWVSASHGIPAEVYLYEKLL 484 KC +V KDA GAV +RCTYDP T+SG S RKVKG +HWVSA H A+V LY++L Sbjct: 420 KCVDVEKDA-EGAVTAVRCTYDPETRSGLPGSERKVKGVIHWVSARHAETAKVRLYDRLF 478 Query: 485 EDNEGPKAEGETWEEYINPDSIRLMLSCLLEPFAAGAKPEDKFQFMRQGYFCVDNKLSRE 544 D P G+ W++++NP S+ ++ C LEP A A+ ED+FQF RQGYFCVD K SR Sbjct: 479 TD---PNPSGDDWKDHLNPASMEILADCRLEPSLATAQAEDRFQFERQGYFCVDLKDSRP 535 Query: 545 DRLVFNRIVPLKDTW 559 VFNR V L+D+W Sbjct: 536 GAPVFNRTVTLRDSW 550 >ref|ZP_00124513.1| COG0008: Glutamyl- and glutaminyl-tRNA synthetases [Pseudomonas syringae pv. syringae B728a] Length = 569 Score = 601 bits (1549), Expect = e-170, Method: Compositional matrix adjust. Identities = 296/551 (53%), Positives = 386/551 (70%), Gaps = 5/551 (0%) Query: 10 AAAENYMDKLIKEDIESGIYSREICTRFPPEPNGYLHIGSAFAIHTNYGLAHKYGGTFHL 69 A N++ +++ D++SG +S+ I TRFPPEPNGYLHIG A +I N+GLA ++GG HL Sbjct: 22 AVPTNFLRPIVQADLDSGKHSK-IVTRFPPEPNGYLHIGHAKSICVNFGLAQEFGGDTHL 80 Query: 70 RFDDTNPLKEDMEYVKAIIQDIEWLGCSPGEHIYYGSDYSEQIYNSALNLIRKGKAYVCD 129 RFDDTNP KED EY+ AI+ D++WLG + Y S Y +Q+++ A+ LI+ GKAYV D Sbjct: 81 RFDDTNPAKEDQEYIDAIMSDVKWLGFEWAGEVRYASQYFDQLHDWAVELIKAGKAYVDD 140 Query: 130 LSPEEVTAYRGTLTEPGKNSPYRERSVEENLRLFSGMREGAYPTGSRVLRAKIDMSSPNI 189 L+PE+ YRGTLTEPGKNSP+RERSVEENL LF+ M+ G + G+RVLRAKIDM+SPN+ Sbjct: 141 LTPEQAREYRGTLTEPGKNSPFRERSVEENLDLFARMKAGEFEDGARVLRAKIDMASPNM 200 Query: 190 NLRDPVIYRIIHAEHYRTGNAWCIYPMYDFAHPIQDAIEGITHSLCSLEFKDHRPLYEWV 249 NLRDP+IYRI HA H++TG+ WCIYP+YDF H DA+EGITHS+C+LEF+ HRPLY+W Sbjct: 201 NLRDPIIYRIRHAHHHQTGDKWCIYPIYDFTHGQSDALEGITHSICTLEFESHRPLYDWF 260 Query: 250 LEELGIEEAPRQREFGRVNLTGVVTGKRYLRALVAGNYVDGWDDXXXXXXXXXXXXXXXX 309 L+ L + PRQ EF R+NL +T KR L+ LV +V+GWDD Sbjct: 261 LDNLPVPCKPRQYEFSRLNLNYTITSKRKLKQLVDEKHVNGWDDPRMSTLSGFRRRGYTP 320 Query: 310 ESIRSFVREIGMVRNQTMVDFSMLEHCLRQDLKASVPSIMAVLDPLKVVITNYEAGETEW 369 +SIR+F IG R+ +VDF MLE +R DL S P M VL PLKVVITNY G+ E Sbjct: 321 KSIRNFCEMIGTNRSDGVVDFGMLEFSIRDDLDHSAPRAMCVLRPLKVVITNYPEGQVEK 380 Query: 370 LPLANNSENAELGVREVPFSGTVYIERDDFMEEPAPGFRRLVPGGEVRLKGAYIIKCNEV 429 L L + + +LG+RE+PFS +YI+RDD+MEEP G++RL P GEVRL+G+Y+I+ +E Sbjct: 381 LELPRHPKE-DLGMRELPFSRELYIDRDDYMEEPPKGYKRLEPNGEVRLRGSYVIRADEA 439 Query: 430 IKDAGTGAVRELRCTYDPLTKSGGANSGRKVKGTLHWVSASHGIPAEVYLYEKLLEDNEG 489 IKDA G + ELRC+YDP T G GRKVKG +HWV A+ + EV LY++L Sbjct: 440 IKDA-DGNIVELRCSYDPET-LGKNPEGRKVKGVVHWVPAAESVECEVRLYDRLFRSPNP 497 Query: 490 PKA-EGETWEEYINPDSIRLMLSCLLEPFAAGAKPEDKFQFMRQGYFCVDNKLSREDRLV 548 KA EG ++ + INPDS++++ C EP A A+PED+FQF R+GYFC D K SR R V Sbjct: 498 EKAEEGASFLDNINPDSLQVLTGCRAEPSLAQAQPEDRFQFEREGYFCADIKDSRPGRPV 557 Query: 549 FNRIVPLKDTW 559 FNR V L+D+W Sbjct: 558 FNRTVTLRDSW 568 >ref|ZP_00097505.1| COG0008: Glutamyl- and glutaminyl-tRNA synthetases [Desulfitobacterium hafniense] Length = 540 Score = 598 bits (1541), Expect = e-169, Method: Compositional matrix adjust. Identities = 298/559 (53%), Positives = 381/559 (68%), Gaps = 21/559 (3%) Query: 1 MGNDNAMETAAAENYMDKLIKEDIESGIYSREICTRFPPEPNGYLHIGSAFAIHTNYGLA 60 M N+N + N++ +I ED+ G EI TRFPPEPNGYLHIG A +I N+GLA Sbjct: 1 MDNENK-----SSNFLRSIILEDLREGKVE-EITTRFPPEPNGYLHIGHAKSICLNFGLA 54 Query: 61 HKYGGTFHLRFDDTNPLKEDMEYVKAIIQDIEWLGCSPGEHIYYGSDYSEQIYNSALNLI 120 ++ G +LRFDDTNP+KED EYV++I +D++WLG +++++ SDY +++ A+ LI Sbjct: 55 DEFKGKTNLRFDDTNPVKEDTEYVESIKEDVKWLGFD-WDNLFFASDYFAEMHKRAVLLI 113 Query: 121 RKGKAYVCDLSPEEVTAYRGTLTEPGKNSPYRERSVEENLRLFSGMREGAYPTGSRVLRA 180 KGKAYVCDLS EE+ RGTLTE GK SPYR RSV ENL LF MR+G + G +VLRA Sbjct: 114 EKGKAYVCDLSAEEIRKSRGTLTEAGKESPYRNRSVAENLELFERMRQGEFKDGEKVLRA 173 Query: 181 KIDMSSPNINLRDPVIYRIIHAEHYRTGNAWCIYPMYDFAHPIQDAIEGITHSLCSLEFK 240 KIDM SPNIN+RDPV+YRI HA H+ TG+ WCIYPMYD+AHP++DAIEGITHS+C+LEF+ Sbjct: 174 KIDMGSPNINMRDPVLYRIAHATHHNTGDTWCIYPMYDYAHPLEDAIEGITHSICTLEFE 233 Query: 241 DHRPLYEWVLEELGIEEAPRQREFGRVNLTGVVTGKRYLRALVAGNYVDGWDDXXXXXXX 300 DHRPLY+WV+ E +E P Q EF R+N+T V KR L+ LV VDGWDD Sbjct: 234 DHRPLYDWVVRECEMEHVPHQYEFARLNMTNTVMSKRKLKQLVDEKVVDGWDDPRMPTIS 293 Query: 301 XXXXXXXXXESIRSFVREIGMVRNQTMVDFSMLEHCLRQDLKASVPSIMAVLDPLKVVIT 360 E+IR+F + IG+ R ++VD MLEH +R+DLK V MAV+ PLKVVIT Sbjct: 294 GLRRRGYTPEAIRNFAQAIGVARADSVVDSKMLEHFIREDLKLKVSRPMAVIRPLKVVIT 353 Query: 361 NYEAGETEWLPLANNSENAELGVREVPFSGTVYIERDDFMEEPAPGFRRLVPGGEVRLKG 420 NY G+ E L +N EN E+G R++PFS +YIE++DFME P P + RL PG EVRL Sbjct: 354 NYPEGQVERLDADHNKENPEMGKRKIPFSREIYIEQEDFMEVPPPKYHRLYPGNEVRLMD 413 Query: 421 AYIIKCNEVIKDAGTGAVRELRCTYDPLTKSGGANSGRKVKGTLHWVSASHGIPAEVYLY 480 AY IKC EVIKD G + E+RCTYDP TKSG +GRKVKGT+HWV A+ +PAE LY Sbjct: 414 AYFIKCEEVIKD-DEGKIIEIRCTYDPETKSGTGFTGRKVKGTIHWVEATQAVPAEFRLY 472 Query: 481 EKLLEDNEGPKAEGETWEEYINPDSIRLMLSCLLEPFAAGAKPEDKFQFMRQGYFCVDNK 540 E L++D+ YINP+S+ + +EP+ AKP+DKFQF R GYF VD Sbjct: 473 EPLIQDD------------YINPNSLEIR-QGFVEPYMKEAKPQDKFQFPRHGYFNVDPN 519 Query: 541 LSREDRLVFNRIVPLKDTW 559 + + LV N IV LK ++ Sbjct: 520 YTTPESLVLNLIVSLKSSF 538 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.137 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 900,754,783 Number of extensions: 40187193 Number of successful extensions: 73576 Number of sequences better than 1.0e-05: 858 Number of HSP's gapped: 72307 Number of HSP's successfully gapped: 861 Length of query: 563 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 432 Effective length of database: 401,559,609 Effective search space: 173473751088 Effective search space used: 173473751088 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)