BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0018.p (670 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|EAI76722.1| unknown [environmental sequence] 196 2e-48 ref|NP_624209.1| Aminopeptidase N [Thermoanaerobacter tengcongen... 183 1e-44 gb|EAH91188.1| unknown [environmental sequence] 158 3e-37 gb|EAH91191.1| unknown [environmental sequence] 155 2e-36 gb|EAI67990.1| unknown [environmental sequence] 154 6e-36 >gb|EAI76722.1| unknown [environmental sequence] Length = 707 Score = 196 bits (497), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 167/602 (27%), Positives = 273/602 (45%), Gaps = 115/602 (19%) Query: 73 QRVTEYHLEAQLLPSTGSLTASETVTWTHPGRKPVQDLYFHLYPNAFASKDTTFMKESGG 132 Q+V Y ++ L +L+A E + + + + ++FH++PNA+ + T + K++G Sbjct: 40 QQVVNYDIDVSLDDEKHTLSAYEKIEYKNNSPDELSFIWFHVWPNAYKNDSTAYAKQAGP 99 Query: 133 KLRGDTMPANGYGSMTLTDLRTS-DGISLMQRLQYVQPDDGNVHDKTLVKVHLPQPVNGG 191 R + G + D R + ++ +++ +VK++L P+ Sbjct: 100 NSRFAKSDSTERGYIDSLDFRVNGKKVNWSYHPEWID----------VVKLNLATPLKPA 149 Query: 192 ESVTLKLKFTVALP-KIFARMGVSGNFIMAGQWFPKISVYEPAGTRGVKQEGWNLHQYHG 250 E++ ++ F V LP ++F+R+G +G QW+PK +VY+ + GW+ Y Sbjct: 150 ETIIIETPFFVKLPDEVFSRLGHTGKHYEITQWYPKPAVYD--------KNGWHPMPYLN 201 Query: 251 TSEFYSDFGIYNVTLSVPSNYTVAATGFPVKTAK----------------------LKQG 288 EFYS+FG ++V +++P NY V ATG V K LK+ Sbjct: 202 QGEFYSEFGTFDVKITLPENYKVMATGDLVNGEKEYLWLEGLTAITDSLNALPKKELKKW 261 Query: 289 QKIYK-------------------FYADDVHDFAWAASPDFLV--AEEAFSSSEVPGVRI 327 K+ K F ++VHDFAW A +LV E + S P Sbjct: 262 IKVQKESLKKSNTDSSNSKFKTLHFRQENVHDFAWFADKKWLVQKGELSLPSRSDPITLW 321 Query: 328 KLYLDPLHKDLQERYFQAAKAALNTFSKWYGPYPYSTLSIVVPPKEGSGAGGMEYPTL-V 386 YL P + +L + + FSK+YG YPY+ ++ V + S GGMEYP + V Sbjct: 322 SFYL-PKNAELWRNSIEYLHDSGYWFSKYYGDYPYNHITAV--DGDLSAGGGMEYPNITV 378 Query: 387 TASSASENVPGTQLERTIIHEIGHQFFYGMVASNEFEEAWLDESFTSYAEDRLMEQEY-- 444 AS S+++ LE I+HE+GH +FYG++ SNE W+DE Y+ R + +Y Sbjct: 379 IASMPSKHL----LELVIMHEVGHNWFYGIIGSNERYHTWMDEGLNDYSNIRYWQDKYKD 434 Query: 445 ---------------GVASNLP------LQ-ASLVPS--AQPLTLETWKYTGDDAYTRNI 480 G+A +L LQ AS S QPL L+ +YT + Y N Sbjct: 435 TNERLVVFEPIQDKLGIAKSLQYSWFSYLQYASSAKSNNVQPLNLKADEYTSSN-YGLN- 492 Query: 481 YIRGKLVLKDIEHQVGTKTMDAILAAYARKYRFRHPSTADFQRTVEKVTKKSWQTYFDHY 540 Y + + + + H +G + MD I+ Y RK+++RHP+ DF+ + T K+ YF Sbjct: 493 YSKTGVFTRFLHHYLGDEKMDEIMQEYYRKWKYRHPTPVDFKAVFDSKTNKNLNWYFK-- 550 Query: 541 VYSGGAPDFAINHIANTISKAGADNEDSRYESSVNVSNKGSWYGDVPVKFTFSDGYTVQR 600 GA + I++I I+K G + VSN G+ V V F + ++R Sbjct: 551 ----GALE-TIDYIDYAITKKG---------NQFIVSNNGTLNTPVEVVFYGKNKNEIER 596 Query: 601 YW 602 W Sbjct: 597 TW 598 >ref|NP_624209.1| Aminopeptidase N [Thermoanaerobacter tengcongensis] gb|AAM25813.1| Aminopeptidase N [Thermoanaerobacter tengcongensis] Length = 479 Score = 183 bits (465), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 139/486 (28%), Positives = 226/486 (46%), Gaps = 54/486 (11%) Query: 75 VTEYHLEAQLLPSTGSLTASETVTWTHPGRKPVQDLYFHLYPNAFASKDTT-FMKESGGK 133 +T Y +EA+ + ++ A +TV + + ++++ FHLYPNAF S++ F KE Sbjct: 30 MTTYLIEAKYDDGSKTVEAVQTVRYVNTTGASLKEICFHLYPNAFKSEENLPFTKEE--- 86 Query: 134 LRGDTMPANGYGSMTLTDLRTSDGISLMQRLQYVQPDDGNVHDKTLVKVHLPQPVNGGES 193 P + T +G+ + L+ +P+D ++++ LP+ + GES Sbjct: 87 -LPFVYPEGFAPGFIEIEKVTINGLPAVYTLE--EPND------EILRLTLPREIKKGES 137 Query: 194 VTLKLKFTVALPKIFARMGVSGNFIMAGQWFPKISVYEPAGTRGVKQEGWNLHQYHGTSE 253 V +K+++ V LP +R G N + G W+P +SVY+ + GWN Y+ + Sbjct: 138 VNIKMQYKVKLPPARSRFGYGKNTVQLGNWYPILSVYD--------KNGWNNDPYYIFGD 189 Query: 254 -FYSDFGIYNVTLSVPSNYTVAATGFPVKTAKLKQGQKIYKFYADDVHDFAWAASPDFLV 312 FYSD Y V L+VP + VA TG K K+++G K A V DFA S F V Sbjct: 190 PFYSDIADYKVELTVPEKFVVACTG-EEKEQKVEKGSKKLVIEAHKVRDFAIVMSDKFKV 248 Query: 313 AEEAFSSSEVPGVRIKLYLDPLHKDLQERYFQAAKAALNTFSKWYGPYPYSTLSIVVPPK 372 E+ V G+++K Y + ++ + A A+ ++ + G YPY S+V + Sbjct: 249 IEQV-----VDGIKVKSYY--FTEGYGDKVLKFASDAIKFYNDYIGKYPYKEYSVV---E 298 Query: 373 EGSGAGGMEYPTLVTASSASENVPGT-QLERTIIHEIGHQFFYGMVASNEFEEAWLDESF 431 GGMEYP LV S N LE I HE HQ++Y +V +N+ E+WLDE Sbjct: 299 TDFYMGGMEYPQLVMISKDLYNKANLFNLEYVIAHETAHQWWYALVGNNQIRESWLDEGL 358 Query: 432 TSYAEDRLMEQEYGVASNLPLQASLVPS--------------AQPLT-LETWKYTGDDAY 476 T Y +E+ YG A+ + ++ A+ L + WK Y Sbjct: 359 TEYTTLLYIERYYGKATAEEIYRLVIEGGFNKFVNNSADTSFAKTLADFKEWK-----EY 413 Query: 477 TRNIYIRGKLVLKDIEHQVGTKTMDAILAAYARKYRFRHPSTADFQRTVEKVTKKSWQTY 536 T Y +G +V ++ +G + +L Y KY+++ +T D V+ VT K + Sbjct: 414 TNIAYNKGAMVFYNLRKLIGDEDFKKVLQTYYEKYKYKIATTQDLIDVVDSVTGKDTGGF 473 Query: 537 FDHYVY 542 F + Y Sbjct: 474 FQSWFY 479 >gb|EAH91188.1| unknown [environmental sequence] Length = 571 Score = 158 bits (400), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 133/527 (25%), Positives = 238/527 (45%), Gaps = 80/527 (15%) Query: 65 LPVSEALSQRVTEYHLEAQLLPSTGSLTASETVTWTHPGRKPVQDLYFHLYPNAFASKDT 124 L S+ Q+ EY ++ + S + +++ +T+ ++ +Y+HL+ NAF Sbjct: 13 LSFSQNYWQQHVEYEMDIVVDVSDFTYDGDQSIIYTNNSNDTIKKVYYHLFFNAFKPNSQ 72 Query: 125 TFMKESGGK-------LRGDTMPANGYGSMTLTDLRTSDGISLMQRLQYVQPDDGNVHDK 177 ++ + R + YG ++++ L+ DG + + ++ Sbjct: 73 MDIRSRTIRDPDRRVGSRIVALEEKDYGDISVSALK-QDGKDVYYEV-----------NE 120 Query: 178 TLVKVHLPQPVNGGESVTLKLKFTVALPKIFARMGV---SGNFIMAGQWFPKISVYEPAG 234 T++ L +P+ G+ L + FT +P R G G + QWFPK++ Y+ Sbjct: 121 TVLLARLNKPLLPGKKTKLNMVFTAQVPLQIRRSGKLNKEGVDMTMTQWFPKLAEYD--- 177 Query: 235 TRGVKQEGWNLHQYHGTSEFYSDFGIYNVTLSVPSNYTVAATGFPVKTAKLKQG------ 288 EGW+ + Y G EF+ +G Y+V +++ +Y V TG+ ++ G Sbjct: 178 -----SEGWHPNPYIG-REFHGVWGNYSVNITIDKDYVVGGTGYLQNANEIGHGYTEKEP 231 Query: 289 ------QKIYKFYADDVHDFAWAASPDFLVAEEAFSSSEVPGVRIKLYLDPL-----HKD 337 +KFYA +VHDFAWAA PD++ SE GV + + P K Sbjct: 232 KENDSKTNTWKFYAPNVHDFAWAADPDYI---HDIKKSE-SGVDLHFFYKPTVNVEDWKK 287 Query: 338 LQERYFQAAKAALNTFSKWYGPYPYSTLSIVVPPKEGSGAGGMEYP--TLVTASSASENV 395 LQE + + F + GPYP+ SI+ G GGMEY T++T + ++ Sbjct: 288 LQE----DSVLLMKYFEEAIGPYPWKQYSII-----QGGDGGMEYAMCTMITGERSYPSL 338 Query: 396 PGTQLERTIIHEIGHQFFYGMVASNEFEEAWLDESFTSYAEDRLMEQEYGVASN--LPLQ 453 G HE+ H +F ++A+NE + AW+DE FT Y + + Y N P + Sbjct: 339 LGV-----TAHEMAHAWFQHLLATNEAKHAWMDEGFTEYITS--LSENYVNNENPEFPHK 391 Query: 454 AS------LVPSA--QPLTLETWKYTGDDAYTRNIYIRGKLVLKDIEHQVGTKTMDAILA 505 +S L S Q T+ + +Y + AY + Y +G + L + + +G + ++ L Sbjct: 392 SSYDRYYLLAKSGFEQAQTIHSDRYDYNFAYGASAYSKGSVFLSQLGYIIGKENLNKTLR 451 Query: 506 AYARKYRFRHPSTADFQRTVEKVTKKSWQTYFDHYVYSGGAPDFAIN 552 Y +++F+HP+ DF+R EKV+ + Y + + + D+ I+ Sbjct: 452 RYYEEFKFKHPTPNDFKRIAEKVSDLELEWYLNEWTRTPHKIDYGID 498 >gb|EAH91191.1| unknown [environmental sequence] Length = 595 Score = 155 bits (393), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 128/521 (24%), Positives = 231/521 (44%), Gaps = 68/521 (13%) Query: 65 LPVSEALSQRVTEYHLEAQLLPSTGSLTASETVTWTHPGRKPVQDLYFHLYPNAFASKDT 124 L S+ Q+ EY ++ + S + +++ +T+ ++ +Y+HL+ NAF Sbjct: 10 LSFSQNYWQQHVEYEMDIVVDVSDFTYDGDQSIIYTNNSNDTIKKVYYHLFFNAFKPNSQ 69 Query: 125 TFMKESGGK-------LRGDTMPANGYGSMTLTDLRTSDGISLMQRLQYVQPDDGNVHDK 177 ++ + R + YG ++++ L+ DG + + ++ Sbjct: 70 MDIRSRTIRDPDRRVGSRIVALEEKDYGDISVSALK-QDGKDVYFEV-----------NE 117 Query: 178 TLVKVHLPQPVNGGESVTLKLKFTVALPKIFARMGV---SGNFIMAGQWFPKISVYEPAG 234 T++ L +P+ G+ L + FT +P R G G + QWFPK++ Y+ Sbjct: 118 TVLLARLNKPLLPGKKTKLNMVFTAQVPLQIRRSGKLNKEGVDMTMTQWFPKLAEYD--- 174 Query: 235 TRGVKQEGWNLHQYHGTSEFYSDFGIYNVTLSVPSNYTVAATGFPVKTAKLKQG------ 288 EGW+ + Y G EF+ +G Y+V +++ Y V TG+ ++ G Sbjct: 175 -----SEGWHPNPYIG-REFHGVWGNYSVNITIDKGYVVGGTGYLQNANEIGHGYSEKAP 228 Query: 289 ------QKIYKFYADDVHDFAWAASPDFLVAEEAFSSSEVPGVRIKLYLDPLHKDLQERY 342 +KFYA +VHDFAWAA PD++ SE GV + + P + Sbjct: 229 KENDSKTNTWKFYAPNVHDFAWAADPDYI---HDIKKSE-SGVDLHFFYKPTVNIEDWKK 284 Query: 343 FQAAKAAL-NTFSKWYGPYPYSTLSIVVPPKEGSGAGGMEYP--TLVTASSASENVPGTQ 399 Q L F + GPYP+ SI+ G GGMEY T++T + ++ G Sbjct: 285 LQDDSVLLMKYFEEAIGPYPWKQYSII-----QGGDGGMEYAMCTMITGERSYPSLLGV- 338 Query: 400 LERTIIHEIGHQFFYGMVASNEFEEAWLDESFTSYAEDRLMEQEYGVASNLPLQAS---- 455 HE+ H +F ++A+NE + AW+DE FT Y + P ++S Sbjct: 339 ----TAHEMAHAWFQHLLATNEAKHAWMDEGFTEYITSLSENYVNNESPEFPHKSSYDRY 394 Query: 456 --LVPSA--QPLTLETWKYTGDDAYTRNIYIRGKLVLKDIEHQVGTKTMDAILAAYARKY 511 L S Q T+ + +Y + AY + Y +G + L + + +G + ++ + Y ++ Sbjct: 395 YLLAKSGFEQAQTIHSDRYDYNFAYGASAYSKGSVFLSQLGYIIGKENLNKTIRRYYEEF 454 Query: 512 RFRHPSTADFQRTVEKVTKKSWQTYFDHYVYSGGAPDFAIN 552 +F+HP+ DF+R EKV+ + Y + + + D+ I+ Sbjct: 455 KFKHPAPNDFKRIAEKVSDLELEWYLNEWTRTPHKIDYGID 495 >gb|EAI67990.1| unknown [environmental sequence] Length = 559 Score = 154 bits (389), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 145/596 (24%), Positives = 252/596 (42%), Gaps = 109/596 (18%) Query: 93 ASETVTWTHPGRKPVQDLYFHLYPNAFASKDTTFMKESGGK-------LRGDTMPANGYG 145 +++ +T+ ++ +Y+HL+ NAF ++ + R + YG Sbjct: 2 GDQSIIYTNNSNDTIKKVYYHLFFNAFKPNSQMDIRSRTIRDPDRRVGSRIVALEEKDYG 61 Query: 146 SMTLTDLRTSDGISLMQRLQYVQPDDGNVHDKTLVKVHLPQPVNGGESVTLKLKFTVALP 205 ++++ L+ DG + + ++T++ L +P+ G+ L + FT +P Sbjct: 62 DISVSALK-QDGKDVYYEV-----------NETVLLARLNKPLLPGKKTKLSMVFTAQVP 109 Query: 206 KIFARMGV---SGNFIMAGQWFPKISVYEPAGTRGVKQEGWNLHQYHGTSEFYSDFGIYN 262 R G G + QWFPK++ Y+ EGW+ + Y G EF+ +G Y+ Sbjct: 110 LQIRRSGKLNKEGVDMTMTQWFPKLAEYD--------SEGWHPNPYIG-REFHGVWGNYS 160 Query: 263 VTLSVPSNYTVAATGFPVKTAKLKQG------------QKIYKFYADDVHDFAWAASPDF 310 V +++ +Y V TG+ ++ G +KFYA +VHDFAWAA PD+ Sbjct: 161 VNITIDKDYVVGGTGYLQNANEIGHGYTEKNPKENDSKTNTWKFYAPNVHDFAWAADPDY 220 Query: 311 LVAEEAFSSSEVPGVRIKLYLDPL-----HKDLQERYFQAAKAALNTFSKWYGPYPYSTL 365 + SE GV + + P K LQE + + F + GPYP+ Sbjct: 221 I---HDIKKSE-SGVDLHFFYKPTVNVEDWKKLQE----DSVLLMKYFEEAIGPYPWKQY 272 Query: 366 SIVVPPKEGSGAGGMEYP--TLVTASSASENVPGTQLERTIIHEIGHQFFYGMVASNEFE 423 SI+ G GGMEY T++T + ++ G HE+ H +F ++A+NE + Sbjct: 273 SII-----QGGDGGMEYAMCTMITGERSYPSLLGV-----TAHEMAHAWFQHLLATNEAK 322 Query: 424 EAWLDESFTSYAEDRLMEQEYGVASNLPLQAS------LVPSA--QPLTLETWKYTGDDA 475 AW+DE FT Y + P ++S L S Q T+ + +Y + A Sbjct: 323 HAWMDEGFTEYITSLSENFVNNESPEFPHKSSYDRYYLLAKSGFEQAQTIHSDRYDYNFA 382 Query: 476 YTRNIYIRGKLVLKDIEHQVGTKTMDAILAAYARKYRFRHPSTADFQRTVEKVTKKSWQT 535 Y + Y +G + L + + +G + ++ L Y +++F+HP+ DF+R EKV+ + Sbjct: 383 YGASAYSKGSVFLSQLGYIIGKENLNKTLKRYYDEFKFKHPTPNDFKRIAEKVSDLELEW 442 Query: 536 YFDHYVYSGGAPDFAINHIANTISKAGADNEDSRYESSVNVSNKGSWYGDVPVKFTFSDG 595 Y + + + D+ I+ IS ED R + + K + V TF DG Sbjct: 443 YLNEWTRTPHKIDYGID-----ISSL----EDQR---VITLKRKARIPMPIEVVVTFEDG 490 Query: 596 YTVQRY---------------------WNGESKQTTFQLSYKAPLLSAEIDPEHSV 630 + Y WN S + F++ + EIDP + Sbjct: 491 SSDMYYIPNDLLHGYKTFKNDVYLMEPWNWASTEYEFEVQGNKKVSKVEIDPSKRI 546 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.132 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,003,921,940 Number of extensions: 42981596 Number of successful extensions: 98163 Number of sequences better than 1.0e-05: 234 Number of HSP's gapped: 97983 Number of HSP's successfully gapped: 250 Length of query: 670 Length of database: 757,237,578 Length adjustment: 132 Effective length of query: 538 Effective length of database: 398,844,510 Effective search space: 214578346380 Effective search space used: 214578346380 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)