BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0008.p (508 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|Q9RHV9|SYK_BACST Lysyl-tRNA synthetase (Lysine--tRNA ligase) ... 756 0.0 pir||JC7205 lysine-tRNA ligase (EC 6.1.1.6) - Bacillus stearothe... 756 0.0 ref|NP_387963.1| lysyl-tRNA synthetase [Bacillus subtilis] >gi|5... 734 0.0 ref|NP_240964.1| lysyl-tRNA synthetase [Bacillus halodurans] >gi... 725 0.0 ref|NP_654026.1| tRNA-synt_2, tRNA synthetases class II (D, K an... 718 0.0 >sp|Q9RHV9|SYK_BACST Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS) dbj|BAA88691.1| lysyl-tRNA synthetase [Geobacillus stearothermophilus] Length = 494 Score = 756 bits (1952), Expect = 0.0, Method: Compositional matrix adjust. Identities = 360/489 (73%), Positives = 420/489 (85%) Query: 18 LSELLTIRRGKLDELRALGIDPFGKKYERTAGAGDIMDKYDGQTKEELDELNLEVSIAGR 77 L++ L +RR KL ++ LG+DPFGK++ERT A ++ + Y +KEEL+E +EV++AGR Sbjct: 6 LNDQLRVRREKLKKIEELGVDPFGKRFERTHKAEELFELYGDLSKEELEEQQIEVAVAGR 65 Query: 78 IMAKRVMGKASFAHIQDLSGKIQIYVRQDSISEAQYAAFTVLDLGDIIGVRGTVFKTRTG 137 IM KR MGKA FAHIQD++G+IQIYVRQD + E QY F + DLGDI+GVRGT+FKT+ G Sbjct: 66 IMTKRGMGKAGFAHIQDVTGQIQIYVRQDDVGEQQYELFKISDLGDIVGVRGTMFKTKVG 125 Query: 138 ETSIKVHNLEVLSKSLLPLPEKYHGLKDVELRYRQRYVDLIINPEVQQTFITRSRIIQSM 197 E SIKV + E L+K+L PLPEKYHGLKD+E RYRQRY+DLI+NPE ++TFITRS IIQSM Sbjct: 126 ELSIKVSSYEFLTKALRPLPEKYHGLKDIEQRYRQRYLDLIMNPESKKTFITRSLIIQSM 185 Query: 198 RRYLDSLGYLEVETPTLHAIAGGAAARPFITHHNTLDMQLYMRIAIELHLKRLIVGGLEK 257 RRYLDS GYLEVETP +HA+AGGAAARPFITHHN LDM LYMRIAIELHLKRLIVGGLEK Sbjct: 186 RRYLDSHGYLEVETPMMHAVAGGAAARPFITHHNALDMTLYMRIAIELHLKRLIVGGLEK 245 Query: 258 VYEIGRVYRNEGISTRHNPEFTMIELYEAYADYKDIMHLTESMIAHIAQEVLGTQKINYQ 317 VYEIGRV+RNEGISTRHNPEFTM+ELYEAYAD++DIM LTE++IAHIA EVLGT KI Y Sbjct: 246 VYEIGRVFRNEGISTRHNPEFTMLELYEAYADFRDIMKLTENLIAHIATEVLGTTKIQYG 305 Query: 318 GQEVDLTPQWRRVSMVDAVKEVTGVDFGVHMTDEEAQRLAKEHRVPVEKHMTFGHILNAF 377 VDLTP+WRR+ MVDA+KE GVDF M+DEEA+ LAKEH V V HMTFGHI+N F Sbjct: 306 EHLVDLTPEWRRLHMVDAIKEYVGVDFWRQMSDEEARELAKEHGVEVAPHMTFGHIVNEF 365 Query: 378 FEQFVEETLIQPTFVTGHPVEISPLAKKNDQDPRFTDRFELFIVAREHANAFSELNDPID 437 FEQ VE+ LIQPTF+ GHPVEISPLAKKN DPRFTDRFELFIV REHANAF+ELNDPID Sbjct: 366 FEQKVEDKLIQPTFIYGHPVEISPLAKKNPDDPRFTDRFELFIVGREHANAFTELNDPID 425 Query: 438 QRQRFEAQIQEKEKGNDEAHEMDDDFIRALEYGMPPTGGLGIGVDRLIMLLTDAASIRDV 497 QRQRFE Q++E+E+GNDEAHEMD+DF+ ALEYGMPPTGGLGIGVDRL+MLLT++ SIRDV Sbjct: 426 QRQRFEEQLKEREQGNDEAHEMDEDFLEALEYGMPPTGGLGIGVDRLVMLLTNSPSIRDV 485 Query: 498 LLFPHMRNR 506 LLFP MR++ Sbjct: 486 LLFPQMRHK 494 >pir||JC7205 lysine-tRNA ligase (EC 6.1.1.6) - Bacillus stearothermophilus Length = 493 Score = 756 bits (1952), Expect = 0.0, Method: Compositional matrix adjust. Identities = 360/489 (73%), Positives = 420/489 (85%) Query: 18 LSELLTIRRGKLDELRALGIDPFGKKYERTAGAGDIMDKYDGQTKEELDELNLEVSIAGR 77 L++ L +RR KL ++ LG+DPFGK++ERT A ++ + Y +KEEL+E +EV++AGR Sbjct: 5 LNDQLRVRREKLKKIEELGVDPFGKRFERTHKAEELFELYGDLSKEELEEQQIEVAVAGR 64 Query: 78 IMAKRVMGKASFAHIQDLSGKIQIYVRQDSISEAQYAAFTVLDLGDIIGVRGTVFKTRTG 137 IM KR MGKA FAHIQD++G+IQIYVRQD + E QY F + DLGDI+GVRGT+FKT+ G Sbjct: 65 IMTKRGMGKAGFAHIQDVTGQIQIYVRQDDVGEQQYELFKISDLGDIVGVRGTMFKTKVG 124 Query: 138 ETSIKVHNLEVLSKSLLPLPEKYHGLKDVELRYRQRYVDLIINPEVQQTFITRSRIIQSM 197 E SIKV + E L+K+L PLPEKYHGLKD+E RYRQRY+DLI+NPE ++TFITRS IIQSM Sbjct: 125 ELSIKVSSYEFLTKALRPLPEKYHGLKDIEQRYRQRYLDLIMNPESKKTFITRSLIIQSM 184 Query: 198 RRYLDSLGYLEVETPTLHAIAGGAAARPFITHHNTLDMQLYMRIAIELHLKRLIVGGLEK 257 RRYLDS GYLEVETP +HA+AGGAAARPFITHHN LDM LYMRIAIELHLKRLIVGGLEK Sbjct: 185 RRYLDSHGYLEVETPMMHAVAGGAAARPFITHHNALDMTLYMRIAIELHLKRLIVGGLEK 244 Query: 258 VYEIGRVYRNEGISTRHNPEFTMIELYEAYADYKDIMHLTESMIAHIAQEVLGTQKINYQ 317 VYEIGRV+RNEGISTRHNPEFTM+ELYEAYAD++DIM LTE++IAHIA EVLGT KI Y Sbjct: 245 VYEIGRVFRNEGISTRHNPEFTMLELYEAYADFRDIMKLTENLIAHIATEVLGTTKIQYG 304 Query: 318 GQEVDLTPQWRRVSMVDAVKEVTGVDFGVHMTDEEAQRLAKEHRVPVEKHMTFGHILNAF 377 VDLTP+WRR+ MVDA+KE GVDF M+DEEA+ LAKEH V V HMTFGHI+N F Sbjct: 305 EHLVDLTPEWRRLHMVDAIKEYVGVDFWRQMSDEEARELAKEHGVEVAPHMTFGHIVNEF 364 Query: 378 FEQFVEETLIQPTFVTGHPVEISPLAKKNDQDPRFTDRFELFIVAREHANAFSELNDPID 437 FEQ VE+ LIQPTF+ GHPVEISPLAKKN DPRFTDRFELFIV REHANAF+ELNDPID Sbjct: 365 FEQKVEDKLIQPTFIYGHPVEISPLAKKNPDDPRFTDRFELFIVGREHANAFTELNDPID 424 Query: 438 QRQRFEAQIQEKEKGNDEAHEMDDDFIRALEYGMPPTGGLGIGVDRLIMLLTDAASIRDV 497 QRQRFE Q++E+E+GNDEAHEMD+DF+ ALEYGMPPTGGLGIGVDRL+MLLT++ SIRDV Sbjct: 425 QRQRFEEQLKEREQGNDEAHEMDEDFLEALEYGMPPTGGLGIGVDRLVMLLTNSPSIRDV 484 Query: 498 LLFPHMRNR 506 LLFP MR++ Sbjct: 485 LLFPQMRHK 493 >ref|NP_387963.1| lysyl-tRNA synthetase [Bacillus subtilis] sp|P37477|SYK_BACSU Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS) pir||S66111 lysine-tRNA ligase (EC 6.1.1.6) lysS - Bacillus subtilis dbj|BAA05316.1| lysyl-tRNA thynthetase [Bacillus subtilis] emb|CAB11858.1| lysyl-tRNA synthetase [Bacillus subtilis subsp. subtilis str. 168] Length = 499 Score = 734 bits (1895), Expect = 0.0, Method: Compositional matrix adjust. Identities = 345/489 (70%), Positives = 413/489 (84%) Query: 18 LSELLTIRRGKLDELRALGIDPFGKKYERTAGAGDIMDKYDGQTKEELDELNLEVSIAGR 77 L++ L +RR K+++LR GIDPFG ++ERT + +++ Y TKEEL+E +EV+IAGR Sbjct: 11 LNDQLQVRRDKMNQLRDNGIDPFGARFERTHQSQEVISAYQDLTKEELEEKAIEVTIAGR 70 Query: 78 IMAKRVMGKASFAHIQDLSGKIQIYVRQDSISEAQYAAFTVLDLGDIIGVRGTVFKTRTG 137 +M KR GKA FAH+QDL G+IQIYVR+DS+ + QY F DLGD+IGV G VFKT G Sbjct: 71 MMTKRGKGKAGFAHLQDLEGQIQIYVRKDSVGDDQYEIFKSSDLGDLIGVTGKVFKTNVG 130 Query: 138 ETSIKVHNLEVLSKSLLPLPEKYHGLKDVELRYRQRYVDLIINPEVQQTFITRSRIIQSM 197 E S+K + E+L+K+L PLP+KYHGLKDVE RYRQRY+DLI+NP+ + TFITRS+IIQ+M Sbjct: 131 ELSVKATSFELLTKALRPLPDKYHGLKDVEQRYRQRYLDLIVNPDSKHTFITRSKIIQAM 190 Query: 198 RRYLDSLGYLEVETPTLHAIAGGAAARPFITHHNTLDMQLYMRIAIELHLKRLIVGGLEK 257 RRYLD GYLEVETPT+H+I GGA+ARPFITHHN LD+ LYMRIAIELHLKRLIVGGLEK Sbjct: 191 RRYLDDHGYLEVETPTMHSIPGGASARPFITHHNALDIPLYMRIAIELHLKRLIVGGLEK 250 Query: 258 VYEIGRVYRNEGISTRHNPEFTMIELYEAYADYKDIMHLTESMIAHIAQEVLGTQKINYQ 317 VYEIGRV+RNEG+STRHNPEFTMIELYEAYADYKDIM LTE+++AHIAQEVLGT I Y Sbjct: 251 VYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMSLTENLVAHIAQEVLGTTTIQYG 310 Query: 318 GQEVDLTPQWRRVSMVDAVKEVTGVDFGVHMTDEEAQRLAKEHRVPVEKHMTFGHILNAF 377 +++DL P+W+R+ MVDAVKE TGVDF +T E+A+ AKEH V ++ MT GHI+N F Sbjct: 311 EEQIDLKPEWKRIHMVDAVKEATGVDFWEEVTVEQAREYAKEHEVEIKDSMTVGHIINEF 370 Query: 378 FEQFVEETLIQPTFVTGHPVEISPLAKKNDQDPRFTDRFELFIVAREHANAFSELNDPID 437 FEQ +EETLIQPTF+ GHPVEISPLAKKN +DPRFTDRFELFIV REHANAF+ELNDPID Sbjct: 371 FEQKIEETLIQPTFIYGHPVEISPLAKKNPEDPRFTDRFELFIVGREHANAFTELNDPID 430 Query: 438 QRQRFEAQIQEKEKGNDEAHEMDDDFIRALEYGMPPTGGLGIGVDRLIMLLTDAASIRDV 497 QR+RFEAQ++E+E GNDEAH MD+DF+ ALEYGMPPTGGLGIG+DRL+MLLT+A SIRDV Sbjct: 431 QRERFEAQLKEREAGNDEAHLMDEDFVEALEYGMPPTGGLGIGIDRLVMLLTNAPSIRDV 490 Query: 498 LLFPHMRNR 506 LLFP MR R Sbjct: 491 LLFPQMRQR 499 >ref|NP_240964.1| lysyl-tRNA synthetase [Bacillus halodurans] sp|Q9KGG4|SYK_BACHD Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS) pir||B83662 lysyl-tRNA synthetase lysS [imported] - Bacillus halodurans (strain C-125) dbj|BAB03817.1| lysyl-tRNA synthetase [Bacillus halodurans] Length = 501 Score = 725 bits (1871), Expect = 0.0, Method: Compositional matrix adjust. Identities = 344/489 (70%), Positives = 413/489 (84%) Query: 18 LSELLTIRRGKLDELRALGIDPFGKKYERTAGAGDIMDKYDGQTKEELDELNLEVSIAGR 77 L++LL +RR KL L A G +PFG K+ERT A + +KYD ++KEEL+E EV +AGR Sbjct: 7 LNDLLAVRREKLQNLEAEGANPFGGKFERTDTAQSMKEKYDAKSKEELEEETHEVVLAGR 66 Query: 78 IMAKRVMGKASFAHIQDLSGKIQIYVRQDSISEAQYAAFTVLDLGDIIGVRGTVFKTRTG 137 +M KR GKA FAHIQDL+G+IQIYVR+D++ + Y FT +D+GDI+GV+G FKT+ G Sbjct: 67 VMTKRGKGKAGFAHIQDLTGQIQIYVRKDAVGDKSYDLFTSIDIGDIVGVKGVAFKTKVG 126 Query: 138 ETSIKVHNLEVLSKSLLPLPEKYHGLKDVELRYRQRYVDLIINPEVQQTFITRSRIIQSM 197 E S+KV + ++L+KSL PLP+KYHGLKDVE RYRQRY+DLI+NPEV+ TF+ RS+I+QSM Sbjct: 127 ELSVKVQDFQLLTKSLRPLPDKYHGLKDVEQRYRQRYLDLIVNPEVRDTFVLRSKILQSM 186 Query: 198 RRYLDSLGYLEVETPTLHAIAGGAAARPFITHHNTLDMQLYMRIAIELHLKRLIVGGLEK 257 RRYLD GYLEVETPT+H+I GGA+ARPF+THHN LDM LYMRIAIELHLKRLIVGG+EK Sbjct: 187 RRYLDDRGYLEVETPTMHSIPGGASARPFVTHHNALDMTLYMRIAIELHLKRLIVGGMEK 246 Query: 258 VYEIGRVYRNEGISTRHNPEFTMIELYEAYADYKDIMHLTESMIAHIAQEVLGTQKINYQ 317 VYEIGRV+RNEG+STRHNPEFTMIELYEAYADY DIM LTE +IAHIA++VLGT I Y Sbjct: 247 VYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYNDIMELTELLIAHIAKDVLGTTTITYG 306 Query: 318 GQEVDLTPQWRRVSMVDAVKEVTGVDFGVHMTDEEAQRLAKEHRVPVEKHMTFGHILNAF 377 EV+L P+WRRV MV+A+KE TGVDF M+DEEA+ LAKEH VPV++ MT+GH++N F Sbjct: 307 EHEVNLEPKWRRVHMVEAIKEETGVDFWNEMSDEEARALAKEHNVPVKETMTYGHVVNEF 366 Query: 378 FEQFVEETLIQPTFVTGHPVEISPLAKKNDQDPRFTDRFELFIVAREHANAFSELNDPID 437 FE FVEE LIQPTFV GHPV ISPLAKKN DPRFTDRFELFIV REHANAFSELNDPID Sbjct: 367 FEHFVEEKLIQPTFVYGHPVAISPLAKKNPDDPRFTDRFELFIVGREHANAFSELNDPID 426 Query: 438 QRQRFEAQIQEKEKGNDEAHEMDDDFIRALEYGMPPTGGLGIGVDRLIMLLTDAASIRDV 497 QR RFE Q+ E+E+G+DEAH MD+DF+ +LEYGMPPTGGLGIG+DRL+MLLT++ SIRDV Sbjct: 427 QRHRFEQQLVEREQGDDEAHMMDEDFVESLEYGMPPTGGLGIGIDRLVMLLTNSPSIRDV 486 Query: 498 LLFPHMRNR 506 LLFP MR++ Sbjct: 487 LLFPQMRHK 495 >ref|NP_654026.1| tRNA-synt_2, tRNA synthetases class II (D, K and N) [Bacillus anthracis A2012] ref|NP_842645.1| lysyl-tRNA synthetase [Bacillus anthracis str. Ames] sp|Q81VW3|SYK_BACAA Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS) gb|AAP24131.1| lysyl-tRNA synthetase [Bacillus anthracis str. Ames] Length = 499 Score = 718 bits (1854), Expect = 0.0, Method: Compositional matrix adjust. Identities = 340/491 (69%), Positives = 408/491 (83%) Query: 18 LSELLTIRRGKLDELRALGIDPFGKKYERTAGAGDIMDKYDGQTKEELDELNLEVSIAGR 77 L++ L +RR KL LR GIDPFGK++ERT D++ Y +KEEL+E + VSIAGR Sbjct: 9 LNDQLLVRREKLHNLREQGIDPFGKRFERTNATNDLLSLYGEFSKEELEEKEISVSIAGR 68 Query: 78 IMAKRVMGKASFAHIQDLSGKIQIYVRQDSISEAQYAAFTVLDLGDIIGVRGTVFKTRTG 137 IM KR GKA FAHIQDL G++QIYVR+D++ + +Y F DLGD++G+ G VFKT G Sbjct: 69 IMTKRGKGKAGFAHIQDLHGQVQIYVRKDAVGDEEYELFKTADLGDLVGIEGKVFKTNVG 128 Query: 138 ETSIKVHNLEVLSKSLLPLPEKYHGLKDVELRYRQRYVDLIINPEVQQTFITRSRIIQSM 197 E S+K +L+KSL PLP+KYHGLKDVE RYRQRY+DLI + E ++TF+TRS+II+ M Sbjct: 129 ELSVKATGFTLLTKSLRPLPDKYHGLKDVEQRYRQRYLDLITSMESRETFVTRSKIIREM 188 Query: 198 RRYLDSLGYLEVETPTLHAIAGGAAARPFITHHNTLDMQLYMRIAIELHLKRLIVGGLEK 257 RRYLD GYLEVETP +HAIAGGA+ARPFITHHN LDM+LYMRIAIELHLKRLIVGGLEK Sbjct: 189 RRYLDDNGYLEVETPMMHAIAGGASARPFITHHNALDMELYMRIAIELHLKRLIVGGLEK 248 Query: 258 VYEIGRVYRNEGISTRHNPEFTMIELYEAYADYKDIMHLTESMIAHIAQEVLGTQKINYQ 317 VYEIGRV+RNEG+STRHNPEFTMIELYEAYADYKDIM LTE+M+AHIA++VLGT I Y Sbjct: 249 VYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTENMVAHIAKQVLGTTTIQYG 308 Query: 318 GQEVDLTPQWRRVSMVDAVKEVTGVDFGVHMTDEEAQRLAKEHRVPVEKHMTFGHILNAF 377 E++L P+W R+ MVDA+KE +G DF M+ EEA+ LAKEH V ++ M GHI+N F Sbjct: 309 DYEINLEPEWTRLHMVDAIKEHSGADFWNPMSVEEARELAKEHNVEIKDTMEVGHIINEF 368 Query: 378 FEQFVEETLIQPTFVTGHPVEISPLAKKNDQDPRFTDRFELFIVAREHANAFSELNDPID 437 FEQ VE+ LIQPTF+ GHPVEISPLAKKND+DPRFTDRFELFIVAREHANAF+ELNDPID Sbjct: 369 FEQKVEDKLIQPTFIYGHPVEISPLAKKNDEDPRFTDRFELFIVAREHANAFTELNDPID 428 Query: 438 QRQRFEAQIQEKEKGNDEAHEMDDDFIRALEYGMPPTGGLGIGVDRLIMLLTDAASIRDV 497 Q++RFEAQ++E+E+GNDEAH MDDD+I ALEYGMPPTGGLGIG+DRL+MLLT+A SIRDV Sbjct: 429 QKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLTNAPSIRDV 488 Query: 498 LLFPHMRNRME 508 LLFP MR++ + Sbjct: 489 LLFPAMRHKQD 499 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.138 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 705,560,355 Number of extensions: 29602686 Number of successful extensions: 79177 Number of sequences better than 1.0e-05: 1326 Number of HSP's gapped: 77643 Number of HSP's successfully gapped: 1394 Length of query: 508 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 378 Effective length of database: 404,274,708 Effective search space: 152815839624 Effective search space used: 152815839624 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)