BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0020.p (74 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_267767.1| hypothetical protein L54040 [Lactococcus lactis... 67 2e-10 ref|NP_721538.1| conserved hypothetical protein [Streptococcus m... 62 5e-09 ref|NP_964155.1| hypothetical protein LJ0139 [Lactobacillus john... 55 4e-07 ref|ZP_00046950.1| COG2151: Predicted metal-sulfur cluster biosy... 54 1e-06 >ref|NP_267767.1| hypothetical protein L54040 [Lactococcus lactis subsp. lactis] pir||C86826 hypothetical protein yqfB [imported] - Lactococcus lactis subsp. lactis (strain IL1403) gb|AAK05709.1| UNKNOWN PROTEIN [Lactococcus lactis subsp. lactis] Length = 79 Score = 66.6 bits (161), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 28/68 (41%), Positives = 46/68 (67%) Query: 5 LNMDESIFELVSRYPEVVDIMVELGFRDITKPGMLQTAGRFMTLSKGITMKKMNREIVRQ 64 L+++ +++L + +PE++D+M +LGF +I PGMLQTAGR T+ G MK ++ + + Sbjct: 4 LDINAKLYDLATEHPEIIDLMDDLGFHEIKMPGMLQTAGRMATIPMGAKMKHIDWDKIVI 63 Query: 65 AFERHGFE 72 AF GFE Sbjct: 64 AFAEAGFE 71 >ref|NP_721538.1| conserved hypothetical protein [Streptococcus mutans UA159] gb|AAN58844.1| conserved hypothetical protein [Streptococcus mutans UA159] Length = 77 Score = 61.6 bits (148), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 25/74 (33%), Positives = 46/74 (62%) Query: 1 MSKGLNMDESIFELVSRYPEVVDIMVELGFRDITKPGMLQTAGRFMTLSKGITMKKMNRE 60 M+ +++ + + + + +PEV DI+VELGF+ + P ML T G+ TL G M ++ E Sbjct: 1 MNNTIDLSKPVAQTIKEHPEVKDILVELGFKPLANPAMLNTVGKVTTLLAGSKMARIPLE 60 Query: 61 IVRQAFERHGFEIM 74 ++Q E +G+E++ Sbjct: 61 KIKQTLEFNGYEVI 74 >ref|NP_964155.1| hypothetical protein LJ0139 [Lactobacillus johnsonii NCC 533] gb|AAS08121.1| hypothetical protein LJ0139 [Lactobacillus johnsonii NCC 533] Length = 196 Score = 55.5 bits (132), Expect = 4e-07, Method: Compositional matrix adjust. Identities = 25/65 (38%), Positives = 39/65 (60%) Query: 10 SIFELVSRYPEVVDIMVELGFRDITKPGMLQTAGRFMTLSKGITMKKMNREIVRQAFERH 69 I +L YP+ + IM + GF I PG+L T GR MT+ G K++ + V+QAFE Sbjct: 131 PIKKLAEEYPDFIQIMYDCGFTRIKIPGLLSTVGRVMTIPLGAQAMKIDLDKVKQAFEDK 190 Query: 70 GFEIM 74 G++++ Sbjct: 191 GYKVI 195 >ref|ZP_00046950.1| COG2151: Predicted metal-sulfur cluster biosynthetic enzyme [Lactobacillus gasseri] Length = 200 Score = 53.9 bits (128), Expect = 1e-06, Method: Compositional matrix adjust. Identities = 24/64 (37%), Positives = 39/64 (60%) Query: 11 IFELVSRYPEVVDIMVELGFRDITKPGMLQTAGRFMTLSKGITMKKMNREIVRQAFERHG 70 I +L YP+ V IM + GF I PG+L T GR MT+ G K++ + +++AFE G Sbjct: 135 IKKLAEEYPDFVQIMYDCGFTRIKIPGLLSTVGRVMTIPLGAQAMKIDLDKIKRAFENKG 194 Query: 71 FEIM 74 ++++ Sbjct: 195 YKVI 198 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.325 0.139 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 92,982,640 Number of extensions: 2660276 Number of successful extensions: 9919 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 9919 Number of HSP's successfully gapped: 4 Length of query: 74 Length of database: 757,237,578 Length adjustment: 44 Effective length of query: 30 Effective length of database: 637,773,222 Effective search space: 19133196660 Effective search space used: 19133196660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 120 (50.8 bits)