BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0011.p (212 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_388292.1| similar to hypothetical proteins [Bacillus subt... 122 7e-27 ref|NP_980488.1| lipase/acylhydrolase, putative [Bacillus cereus... 92 8e-18 ref|NP_833840.1| Lipase/Acylhydrolase with GDSL-like motif [Baci... 89 5e-17 ref|NP_658164.1| hypothetical protein predicted by GeneMark [Bac... 89 5e-17 ref|NP_269570.1| conserved hypothetical protein [Streptococcus p... 83 5e-15 >ref|NP_388292.1| similar to hypothetical proteins [Bacillus subtilis] sp|P42969|YCSK_BACSU Hypothetical protein ycsK pir||I39900 conserved hypothetical protein ycsK - Bacillus subtilis dbj|BAA07363.1| unknown protein [Bacillus subtilis] dbj|BAA09041.1| ycsK [Bacillus subtilis] emb|CAB12218.1| ycsK [Bacillus subtilis subsp. subtilis str. 168] prf||2208409P ORF Length = 213 Score = 122 bits (305), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 75/215 (34%), Positives = 113/215 (52%), Gaps = 9/215 (4%) Query: 1 MSYQYTAIGDSLTTGFGA-LPGNGFVPVYRRMAEGRLREPVGAVNLGINGLTTAALEQRL 59 M +YTA+GDSLTTG G+ L GFV + M E L+ +GL T E+ L Sbjct: 1 MVLRYTALGDSLTTGRGSGLFSPGFVQRFGDMMEADLKTRTAINIFARSGLNT---EEIL 57 Query: 60 G--SDPIFRQSIRNAEIITLSIGGNDLIKAAKAVSFRPGDLSPALARALRECKRNFGGIM 117 G S P ++ IR+A++IT++ GNDLI + ++++ +R C NF ++ Sbjct: 58 GLLSYPYVQKCIRDADMITITGCGNDLIDSV--LAYQTSKDETIFSRVSAHCHENFEKMI 115 Query: 118 GSLRQLKYGSRQPYIIRIVGLYNPYPQLTEATDWVRQFNAFAAGYSSGV-CGFASIYHEF 176 + ++K + PY IR+ LYNP+P++ A W+ FN+ +S A Y F Sbjct: 116 AKVAEIKGENPSPYAIRVFNLYNPFPEIDIAGKWITSFNSHLETLASAPHVKIADAYSIF 175 Query: 177 AGNERSLLSLDHVHPNGRGYRVIAGKLDSLGYWRL 211 G E+ LS D VHPN +GY+ +A + LGY L Sbjct: 176 KGKEQEYLSFDGVHPNSKGYQAMAEAVHKLGYKEL 210 >ref|NP_980488.1| lipase/acylhydrolase, putative [Bacillus cereus ATCC 10987] gb|AAS43096.1| lipase/acylhydrolase, putative [Bacillus cereus ATCC 10987] Length = 259 Score = 92.0 bits (227), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 61/208 (29%), Positives = 103/208 (49%), Gaps = 19/208 (9%) Query: 2 SYQYTAIGDSLTTGFGALPGNGFVPVYRRMAEGRLREPVGAVNLGINGLTTAALEQRLGS 61 S+ + +GDSL G+G+ G GF + + E ++ +P+ NLGINGLTT L +++ S Sbjct: 52 SFYHLVLGDSLAKGYGSTQG-GFAELASKQIEAQIHKPITVENLGINGLTTDRLAKKVES 110 Query: 62 DPIFRQSIRNAEIITLSIGGNDLIKAAKAVSFRPGDLSPALARALRECKRNFGGIMGSLR 121 + + +Q IR A IIT++IGGN+L + + V G + L + K +F + ++ Sbjct: 111 EDV-KQKIRAANIITINIGGNNLFRLNRDVGVIDG------IKMLNKEKAHFEADVKNIV 163 Query: 122 QLKYGSRQPYIIRIVGLYNPYPQLTEATDWVRQFNAFAAGYSSGVCGFASIYH------- 174 + ++ + LYNP QL ++ + F G++ V A Sbjct: 164 KTVRDQNPDALLILSELYNPL-QLDDSI--ASYADMFLDGWNESVYSIAKANQPSIVLPI 220 Query: 175 -EFAGNERSLLSLDHVHPNGRGYRVIAG 201 + N++ L D VHPN +GY +IA Sbjct: 221 RKLISNDKKELLFDQVHPNDKGYSIIAD 248 >ref|NP_833840.1| Lipase/Acylhydrolase with GDSL-like motif [Bacillus cereus ATCC 14579] gb|AAP11041.1| Lipase/Acylhydrolase with GDSL-like motif [Bacillus cereus ATCC 14579] Length = 259 Score = 89.4 bits (220), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 58/209 (27%), Positives = 102/209 (48%), Gaps = 21/209 (10%) Query: 2 SYQYTAIGDSLTTGFGALPGNGFVPVYRRMAEGRLREPVGAVNLGINGLTTAALEQRLGS 61 S+ + +GDSL G+G+ G GF + + E ++ +P+ NLG+NGLTT L +++ S Sbjct: 52 SFYHLVLGDSLAKGYGSTQG-GFAELASKQIEAQIHKPITVENLGVNGLTTDRLAKKVQS 110 Query: 62 DPIFRQSIRNAEIITLSIGGNDLIKAAKAVSFRPGDLSPALARALRECKRNFGGIMGSLR 121 + + ++ IR A IIT++IGGN+L + + V G + L + K +F + S+ Sbjct: 111 EDV-KEKIRAANIITINIGGNNLFRLNRDVGVIDG------IKMLNKEKAHFEADIKSIV 163 Query: 122 QLKYGSRQPYIIRIVGLYNPYPQLTEATDWVRQF-NAFAAGYSSGVCGFASIYH------ 174 + ++ + LYNP + D + + + F G++ V + Sbjct: 164 KTVRDQNPDALLILSELYNPL----QLDDSIASYADMFLDGWNESVYSISKTNQPSIVLP 219 Query: 175 --EFAGNERSLLSLDHVHPNGRGYRVIAG 201 + N++ L D VHPN GY +IA Sbjct: 220 IRKLISNDKKELLFDQVHPNDNGYAIIAN 248 >ref|NP_658164.1| hypothetical protein predicted by GeneMark [Bacillus anthracis A2012] ref|NP_846579.1| lipase/acylhydrolase, putative [Bacillus anthracis str. Ames] gb|AAP28065.1| lipase/acylhydrolase, putative [Bacillus anthracis str. Ames] Length = 259 Score = 89.4 bits (220), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 60/208 (28%), Positives = 102/208 (49%), Gaps = 19/208 (9%) Query: 2 SYQYTAIGDSLTTGFGALPGNGFVPVYRRMAEGRLREPVGAVNLGINGLTTAALEQRLGS 61 S+ + +GDSL G+G+ G GF + E ++ +P+ NLGINGLTT L +++ S Sbjct: 52 SFYHLVLGDSLAKGYGSTQG-GFAELASTQIEAQIHKPITVENLGINGLTTDRLAKKVQS 110 Query: 62 DPIFRQSIRNAEIITLSIGGNDLIKAAKAVSFRPGDLSPALARALRECKRNFGGIMGSLR 121 + + +Q IR A IIT++IGGN+L + + V G + L + K +F + ++ Sbjct: 111 EDV-KQKIRAANIITINIGGNNLFRLNRDVGVIDG------IKMLNKEKAHFEADVKNIV 163 Query: 122 QLKYGSRQPYIIRIVGLYNPYPQLTEATDWVRQFNAFAAGYSSGVCGFASIYH------- 174 + ++ + LYNP QL ++ + F G++ V + Sbjct: 164 KTVRDQNPDALLILSELYNPL-QLDDSI--ASYADMFLDGWNESVYSISKANQPSIVLPI 220 Query: 175 -EFAGNERSLLSLDHVHPNGRGYRVIAG 201 + N++ L D VHPN +GY +IA Sbjct: 221 RKLISNDKKELLFDQVHPNDKGYTIIAD 248 >ref|NP_269570.1| conserved hypothetical protein [Streptococcus pyogenes] ref|NP_607582.1| conserved hypothetical protein [Streptococcus pyogenes MGAS8232] ref|NP_801976.1| conserved hypothetical protein [Streptococcus pyogenes SSI-1] gb|AAK34291.1| conserved hypothetical protein [Streptococcus pyogenes M1 GAS] gb|AAL98081.1| conserved hypothetical protein [Streptococcus pyogenes MGAS8232] dbj|BAC63809.1| conserved hypothetical protein [Streptococcus pyogenes SSI-1] Length = 286 Score = 82.8 bits (203), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 60/227 (26%), Positives = 104/227 (45%), Gaps = 33/227 (14%) Query: 1 MSYQYTAIGDSLTTGFGALPGNG-FVPVYRRMAEGRLREPVGAVNLGINGLTTAALEQRL 59 ++ QY AIGDSLT G G L G FVP+ + V N G++G T+ + R+ Sbjct: 52 VAIQYVAIGDSLTEGVGDLTHQGGFVPLLTNDLSEYFKANVNHQNYGVSGDTSQQILDRM 111 Query: 60 GSDPIFRQSIRNAEIITLSIGGNDLIKAAKAVSFRPGDLSPALARALRECKRNFGGIMGS 119 + S++ A+I+TL++GGND++ + +L+ + R+ R + + Sbjct: 112 IKQKQIQLSLKKADIMTLTVGGNDVMAVIRK------NLADLQVSSFRKPARQYQKRLRQ 165 Query: 120 LRQLKYGSRQPYIIRIVGLYNP----YPQLTEA----TDWVRQFNAFAAGYS-------- 163 + +L + I I+G+YNP +P+LT+ DW + Y Sbjct: 166 IIELARKDNKDLPIFILGIYNPFYLNFPELTDMQKVIDDWNTKTKEVVGEYDRVYFVPIN 225 Query: 164 ----SGVCGFASIYHEFAGNERS-----LLSLDHVHPNGRGYRVIAG 201 G+ G I H +G++ + L + DH HPN GY++++ Sbjct: 226 DLLYKGINGQEGIVHS-SGDQTTIVNDALFTGDHFHPNNTGYQIMSN 271 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.140 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 325,857,496 Number of extensions: 13120216 Number of successful extensions: 27237 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 27207 Number of HSP's successfully gapped: 26 Length of query: 212 Length of database: 757,237,578 Length adjustment: 120 Effective length of query: 92 Effective length of database: 431,425,698 Effective search space: 39691164216 Effective search space used: 39691164216 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 123 (52.0 bits)