BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0002.p (77 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_391243.1| yvaL [Bacillus subtilis] >gi|6226126|sp|O32233|... 53 2e-06 >ref|NP_391243.1| yvaL [Bacillus subtilis] sp|O32233|SECG_BACSU Probable protein-export membrane protein secG pir||A70028 hypothetical protein yvaL - Bacillus subtilis emb|CAB15368.1| preprotein translocase subunit [Bacillus subtilis subsp. subtilis str. 168] Length = 76 Score = 52.8 bits (125), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 33/73 (45%), Positives = 43/73 (58%) Query: 1 MDIFLKVLLLIFSVGLIAVVLLQKXXXXXXXXXXXXXXEHLFGKTKARGMELVLQRVTVG 60 M L LL+I S+ LI VVLLQ E LFGK KARG++L+L R+TV Sbjct: 1 MHAVLITLLVIVSIALIIVVLLQSSKSAGLSGAISGGAEQLFGKQKARGLDLILHRITVV 60 Query: 61 LAAGFFIMSILVA 73 LA FF+++I +A Sbjct: 61 LAVLFFVLTIALA 73 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.337 0.151 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 64,278,765 Number of extensions: 1275319 Number of successful extensions: 5282 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 5285 Number of HSP's successfully gapped: 8 Length of query: 77 Length of database: 757,237,578 Length adjustment: 47 Effective length of query: 30 Effective length of database: 629,627,925 Effective search space: 18888837750 Effective search space used: 18888837750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.7 bits) S2: 120 (50.8 bits)