BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0013.p (200 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00108714.1| COG1028: Dehydrogenases with different specif... 217 1e-55 ref|NP_103436.1| probable short chain dehydrogenase [Mesorhizobi... 192 3e-48 ref|NP_770660.1| short chain dehydrogenase [Bradyrhizobium japon... 191 8e-48 gb|EAJ36046.1| unknown [environmental sequence] 190 2e-47 ref|NP_869999.1| putative short chain dehydrogenase [Pirellula s... 187 2e-46 >ref|ZP_00108714.1| COG1028: Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) [Nostoc punctiforme] Length = 246 Score = 217 bits (553), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 103/182 (56%), Positives = 134/182 (73%) Query: 18 DISKPAQIEQLYKDTKALFGKIDIVVNNAGIMINSLIGEATEEQFDKQFAINVKGTYFSC 77 DISK I++L++ T FGK+DI+VNNAGI I + +EE FD FAINVKGT+F+C Sbjct: 64 DISKVPDIQRLFEQTLEHFGKVDILVNNAGIAFYKPITQVSEEDFDAIFAINVKGTFFAC 123 Query: 78 QQAFFHLEQGGRIINFSTSVNGQMFPAYSIYAGTKGAVEQFTRQLAKEFGSKGITINAVA 137 QQA HL +GGRIINFS+S M P YS Y GTKGAVEQ TR LAKE G+K I +N ++ Sbjct: 124 QQAAQHLSEGGRIINFSSSTTVMMLPTYSAYVGTKGAVEQITRVLAKELGAKAIAVNVIS 183 Query: 138 PGPVATDLFLEGKSEAQLEGLKKTNAFGRLGQPEDIAGVVSFLAAEQSGWITGQTLRVNG 197 PGP T+LF EGK++ Q++ L + AFG+LG ++IA VV+FLA++++ WITGQ +RVNG Sbjct: 184 PGPTDTELFREGKTQEQIDRLAQMAAFGKLGDVQEIADVVAFLASDEARWITGQNIRVNG 243 Query: 198 GF 199 G Sbjct: 244 GI 245 >ref|NP_103436.1| probable short chain dehydrogenase [Mesorhizobium loti MAFF303099] dbj|BAB49222.1| probable short chain dehydrogenase [Mesorhizobium loti] Length = 258 Score = 192 bits (489), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 94/183 (51%), Positives = 119/183 (65%) Query: 18 DISKPAQIEQLYKDTKALFGKIDIVVNNAGIMINSLIGEATEEQFDKQFAINVKGTYFSC 77 DI+ P I L+ + FG +DI+VNNAGIM S I + FD Q A+N+ G + Sbjct: 76 DIADPTGIATLFDAGEKAFGGVDILVNNAGIMKLSPIAGTDDASFDAQIAVNLGGVFRGT 135 Query: 78 QQAFFHLEQGGRIINFSTSVNGQMFPAYSIYAGTKGAVEQFTRQLAKEFGSKGITINAVA 137 ++ L GGRI+NFS+SV G P Y +YA TK AVE T LAKE G++ +T+NAVA Sbjct: 136 REGAKRLRDGGRIVNFSSSVVGLYQPGYGVYAATKAAVEAMTHILAKELGARRVTVNAVA 195 Query: 138 PGPVATDLFLEGKSEAQLEGLKKTNAFGRLGQPEDIAGVVSFLAAEQSGWITGQTLRVNG 197 PGPV T LF++GKS Q+E + K GRLGQP+DIAGVVSFLA SGW+ GQ +R NG Sbjct: 196 PGPVETALFMDGKSATQIEAIGKMIPLGRLGQPDDIAGVVSFLAGPDSGWVNGQIIRANG 255 Query: 198 GFI 200 G I Sbjct: 256 GVI 258 >ref|NP_770660.1| short chain dehydrogenase [Bradyrhizobium japonicum] dbj|BAC49285.1| short chain dehydrogenase [Bradyrhizobium japonicum USDA 110] Length = 272 Score = 191 bits (485), Expect = 8e-48, Method: Compositional matrix adjust. Identities = 92/183 (50%), Positives = 121/183 (66%) Query: 18 DISKPAQIEQLYKDTKALFGKIDIVVNNAGIMINSLIGEATEEQFDKQFAINVKGTYFSC 77 D++ A +L+ + FG +DI+VNNAGI+ + E T+E F +Q +IN+ + S Sbjct: 90 DLADSATPSRLFDAAERAFGGVDILVNNAGIIELGPLAEVTDEAFARQTSINLGSVFRSL 149 Query: 78 QQAFFHLEQGGRIINFSTSVNGQMFPAYSIYAGTKGAVEQFTRQLAKEFGSKGITINAVA 137 ++A L GGRI++FS+SV G P Y +YA TK AVE T LAKE GS+ IT+NAVA Sbjct: 150 REAARRLRDGGRIVSFSSSVVGLYQPGYGVYAATKAAVETMTHILAKELGSRRITVNAVA 209 Query: 138 PGPVATDLFLEGKSEAQLEGLKKTNAFGRLGQPEDIAGVVSFLAAEQSGWITGQTLRVNG 197 PGPV T LFLEGKSE Q+ + N FGRLGQP+DIAG+V+FL GWI GQ +R NG Sbjct: 210 PGPVETRLFLEGKSEQQVRAIAAMNPFGRLGQPDDIAGIVAFLTGRDGGWINGQVIRANG 269 Query: 198 GFI 200 G + Sbjct: 270 GVV 272 >gb|EAJ36046.1| unknown [environmental sequence] Length = 268 Score = 190 bits (483), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 90/182 (49%), Positives = 120/182 (65%) Query: 18 DISKPAQIEQLYKDTKALFGKIDIVVNNAGIMINSLIGEATEEQFDKQFAINVKGTYFSC 77 +++ PA + L+ + FG +D+VVN+AG+M + I E + D+ AINVKGT+ C Sbjct: 86 NVADPAAVAALFDTARDTFGGLDVVVNSAGVMKLAAIAEFDDAVLDETLAINVKGTFNVC 145 Query: 78 QQAFFHLEQGGRIINFSTSVNGQMFPAYSIYAGTKGAVEQFTRQLAKEFGSKGITINAVA 137 ++A H+ GGRIIN STSV G P+Y +Y +K AVE T+ LA+E +GI +NAVA Sbjct: 146 REAAKHVRDGGRIINLSTSVIGMRMPSYGLYVASKAAVESLTQVLAQEMRGRGIRVNAVA 205 Query: 138 PGPVATDLFLEGKSEAQLEGLKKTNAFGRLGQPEDIAGVVSFLAAEQSGWITGQTLRVNG 197 PGPVAT+LFL+GKS ++ L K N RLGQP+DIA VV+FLA WI GQ LR NG Sbjct: 206 PGPVATELFLQGKSPELVDRLAKLNPLERLGQPDDIARVVAFLAGPDGAWINGQILRANG 265 Query: 198 GF 199 G Sbjct: 266 GM 267 >ref|NP_869999.1| putative short chain dehydrogenase [Pirellula sp. 1] emb|CAD79152.1| putative short chain dehydrogenase [Pirellula sp.] Length = 265 Score = 187 bits (474), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 88/183 (48%), Positives = 119/183 (65%) Query: 18 DISKPAQIEQLYKDTKALFGKIDIVVNNAGIMINSLIGEATEEQFDKQFAINVKGTYFSC 77 D+S A +++++ +A +G +D++VNNAGIM + I + + FD Q A+N +GT+ Sbjct: 83 DVSDSAAVKRMFDSAEAAYGGVDVLVNNAGIMKLASIADCDDAIFDAQVAVNFRGTFNGM 142 Query: 78 QQAFFHLEQGGRIINFSTSVNGQMFPAYSIYAGTKGAVEQFTRQLAKEFGSKGITINAVA 137 ++A L GGRIINFST+V G F Y +YAG K A+E T +AKE + IT+NAVA Sbjct: 143 REAGIRLRDGGRIINFSTTVVGMKFETYGVYAGIKAAIETMTAIMAKEMRGRNITVNAVA 202 Query: 138 PGPVATDLFLEGKSEAQLEGLKKTNAFGRLGQPEDIAGVVSFLAAEQSGWITGQTLRVNG 197 PGP T LFLEGKS+ +E L K + RLG PEDIA VV+FLA W+ GQTLR NG Sbjct: 203 PGPTGTALFLEGKSDELVEKLSKASPLERLGTPEDIANVVAFLAGPDGAWVNGQTLRANG 262 Query: 198 GFI 200 G + Sbjct: 263 GMV 265 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.136 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 257,718,210 Number of extensions: 9905495 Number of successful extensions: 47925 Number of sequences better than 1.0e-05: 6750 Number of HSP's gapped: 40857 Number of HSP's successfully gapped: 6769 Length of query: 200 Length of database: 757,237,578 Length adjustment: 119 Effective length of query: 81 Effective length of database: 434,140,797 Effective search space: 35165404557 Effective search space used: 35165404557 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 123 (52.0 bits)