BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0006.p (303 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_827544.1| putative integral membrane transport protein [S... 290 3e-77 ref|NP_626153.1| putative integral membrane transport protein [S... 285 7e-76 ref|ZP_00006049.1| COG1175: ABC-type sugar transport systems, pe... 199 5e-50 ref|NP_386618.1| PUTATIVE SUGAR TRANSPORT SYSTEM PERMEASE ABC TR... 183 4e-45 ref|NP_357428.1| AGR_L_3272p [Agrobacterium tumefaciens] >gi|179... 177 2e-43 >ref|NP_827544.1| putative integral membrane transport protein [Streptomyces avermitilis MA-4680] dbj|BAC74079.1| putative integral membrane transport protein [Streptomyces avermitilis MA-4680] Length = 314 Score = 290 bits (742), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 139/298 (46%), Positives = 205/298 (68%), Gaps = 7/298 (2%) Query: 8 KRKKGEA------WIPYLLIAPTVLLIAGILIFPIFRVFDLSVQSYDFTRLQDAGYIGLE 61 +R++G A ++PYLLIAP LL+ G + +P+ VF S+++++ T+ GY G + Sbjct: 15 RRRRGSATPRTLPYLPYLLIAPAGLLMLGFIAYPVLSVFYYSLRNFNPTKPWRNGYAGFD 74 Query: 62 NFRHIFTEDDLFYSTLVTTLKWVFSEVALQLVFGLIVALLLNQSFXXXXXXXXXXXXPWA 121 NF IFT+D F+ TL + KWV EV LQL+FGL +AL++NQ+F PWA Sbjct: 75 NFVQIFTKDTQFWDTLTFSAKWVVVEVGLQLLFGLALALIVNQTFVGRALGRALVFSPWA 134 Query: 122 VSGVLTTMLWSLMFNQHIGIINDILLKLGIIHEKIAWLANPDTVFGSVVFAELWRGIPFF 181 VSGVLT+ +W L++N GI L +G+ +WL++ TVF + V A+LWRG+PFF Sbjct: 135 VSGVLTSAIWVLLYNSQTGITR-YLADMGVGEYGTSWLSDTSTVFPAAVIADLWRGVPFF 193 Query: 182 AITLLAALQTIPHEVYESCEVDGAGKLKKLFHITLPYLKESIIFATLLRAIWEFNSIDMI 241 AI +LA LQ++ ++YE+ EVDGAG+L++ FHITLP+LK++I+ +TLLRA+WEFN++D++ Sbjct: 194 AILILADLQSVSKDLYEAAEVDGAGRLRQFFHITLPHLKDAIVLSTLLRAVWEFNNVDLL 253 Query: 242 FTMTNGGPMDMTTTLPIYMMKTSILEGNYGYGSALGVVTFLFLMIFVILYLKLNRAGG 299 +T+T GGP TTTLP+Y+ TS+ N+GY SAL V F L+ F I+YL+L++ GG Sbjct: 254 YTLTGGGPAGETTTLPLYIANTSVDAHNFGYASALTTVAFTILLFFSIVYLRLSKFGG 311 >ref|NP_626153.1| putative integral membrane transport protein [Streptomyces coelicolor A3(2)] pir||T36901 probable integral membrane transport protein - Streptomyces coelicolor emb|CAB46388.1| putative integral membrane transport protein [Streptomyces coelicolor A3(2)] Length = 311 Score = 285 bits (730), Expect = 7e-76, Method: Compositional matrix adjust. Identities = 137/284 (48%), Positives = 198/284 (69%), Gaps = 1/284 (0%) Query: 16 IPYLLIAPTVLLIAGILIFPIFRVFDLSVQSYDFTRLQDAGYIGLENFRHIFTEDDLFYS 75 +PYLLI P LL+ G + +P+ VF S+Q Y+ T+ G+ G +NF IFTED +F+ Sbjct: 26 LPYLLIGPAALLMLGFIAYPVISVFYYSLQEYNPTKPWRNGFAGFDNFVKIFTEDPVFWD 85 Query: 76 TLVTTLKWVFSEVALQLVFGLIVALLLNQSFXXXXXXXXXXXXPWAVSGVLTTMLWSLMF 135 TLV + KWVF EV LQL+FGL +AL++NQ+F PWAVSGVLT+ +W L++ Sbjct: 86 TLVFSAKWVFVEVGLQLLFGLALALIVNQTFVGRGVGRAMVFSPWAVSGVLTSAIWVLLY 145 Query: 136 NQHIGIINDILLKLGIIHEKIAWLANPDTVFGSVVFAELWRGIPFFAITLLAALQTIPHE 195 N G+ L +GI +WL++ TVF + V A+LWRG+PFFAI +LA LQ++ + Sbjct: 146 NSQTGVTR-YLADMGIGSYGTSWLSDTSTVFSAAVVADLWRGVPFFAILILADLQSVSKD 204 Query: 196 VYESCEVDGAGKLKKLFHITLPYLKESIIFATLLRAIWEFNSIDMIFTMTNGGPMDMTTT 255 +YE+ EVDGA +LK+ +HITLP+LK++II +TLLRA+WEFN++D+++T+T GGP TTT Sbjct: 205 LYEAAEVDGASRLKQFWHITLPHLKDAIILSTLLRAVWEFNNVDLLYTLTGGGPAGETTT 264 Query: 256 LPIYMMKTSILEGNYGYGSALGVVTFLFLMIFVILYLKLNRAGG 299 LP+Y+ TS+ ++GY SAL V F+ L+ I+YL+L++ GG Sbjct: 265 LPLYIANTSVDAHDFGYASALTTVAFVILLFCSIVYLRLSKFGG 308 >ref|ZP_00006049.1| COG1175: ABC-type sugar transport systems, permease components [Rhodobacter sphaeroides] Length = 308 Score = 199 bits (507), Expect = 5e-50, Method: Compositional matrix adjust. Identities = 110/292 (37%), Positives = 173/292 (59%), Gaps = 1/292 (0%) Query: 2 EITRQRKRKKGEAWIPYLLIAPTVLLIAGILIFPIFRVFDLSVQSYDFTRLQDAGYIGLE 61 EI R+ + +P L++P VL+ I+ FP+ + +S+ Y R + ++GL+ Sbjct: 11 EIAPARRARLWRQVLPLALLSPAVLVTLAIVFFPMVQTAWMSLHDYVLFRPKKFDWVGLK 70 Query: 62 NFRHIFTEDDLFYSTLVTTLKWVFSEVALQLVFGLIVALLLNQSFXXXXXXXXXXXXPWA 121 ++ I +D++F+ +L T+ W+ V Q + GL ALLLNQ F PWA Sbjct: 71 HYAAIL-QDEVFWISLRHTVLWIAITVPAQALLGLATALLLNQKFPWRPVARALIIIPWA 129 Query: 122 VSGVLTTMLWSLMFNQHIGIINDILLKLGIIHEKIAWLANPDTVFGSVVFAELWRGIPFF 181 + V+ ++W+ +++ + G++ND+LL++GI+ + I WLANP T +++ W+G PFF Sbjct: 130 LPSVVIALMWAWIYDSNYGVLNDLLLRVGILEQSIPWLANPGTALYAIILTLTWQGFPFF 189 Query: 182 AITLLAALQTIPHEVYESCEVDGAGKLKKLFHITLPYLKESIIFATLLRAIWEFNSIDMI 241 A+ +LA LQ+IP YE+ +DGA L++ +HITLP + + A LLR IW NS+D+I Sbjct: 190 AVMILAGLQSIPRSYYEAASLDGASSLRQFWHITLPGISGVLFTAILLRTIWVANSMDVI 249 Query: 242 FTMTNGGPMDMTTTLPIYMMKTSILEGNYGYGSALGVVTFLFLMIFVILYLK 293 F MT GGP T TLP+Y + ++GYGSAL + L L+ VILYLK Sbjct: 250 FVMTGGGPGYATYTLPLYAFLEARTNLDFGYGSALAMTFTLLLLGIVILYLK 301 >ref|NP_386618.1| PUTATIVE SUGAR TRANSPORT SYSTEM PERMEASE ABC TRANSPORTER PROTEIN [Sinorhizobium meliloti] emb|CAC47091.1| PUTATIVE SUGAR TRANSPORT SYSTEM PERMEASE ABC TRANSPORTER PROTEIN [Sinorhizobium meliloti] Length = 311 Score = 183 bits (465), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 99/273 (36%), Positives = 148/273 (54%), Gaps = 2/273 (0%) Query: 1 MEITRQRKRKK-GEAWIPYLLIAPTVLLIAGILIFPIFRVFDLSVQSYDFTRLQDAGYIG 59 + TR K+ +A PYL AP ++LI +++ P+ + + G++G Sbjct: 10 LPATRPPLMKRIADASAPYLYTAPALILIVTVMLVPLVLGISYAFRDIQLLNPFSGGFVG 69 Query: 60 LENFRHIFTEDDLFYSTLVTTLKWVFSEVALQLVFGLIVALLLNQSFXXXXXXXXXXXXP 119 L++FR + +D F+ +L TL W + V LQ FGLI+ALLL++ F P Sbjct: 70 LDHFRAL-AQDQAFFRSLRNTLWWTGASVFLQFAFGLILALLLDKPFHGRAIAQALVFLP 128 Query: 120 WAVSGVLTTMLWSLMFNQHIGIINDILLKLGIIHEKIAWLANPDTVFGSVVFAELWRGIP 179 WAV L + W+ +FN +G + L LGI+ + L++P + A +W GIP Sbjct: 129 WAVPSFLAGLNWAWLFNPVVGPLPHWLFALGIMSQPTNILSDPQLAMWGPIVANIWWGIP 188 Query: 180 FFAITLLAALQTIPHEVYESCEVDGAGKLKKLFHITLPYLKESIIFATLLRAIWEFNSID 239 FFAITLLAALQ IP ++YE+ +DGAG L++ ITLP+L +I LLR +W N D Sbjct: 189 FFAITLLAALQAIPRDLYEAASIDGAGPLQRFLSITLPFLAPTIAITILLRTVWISNFAD 248 Query: 240 MIFTMTNGGPMDMTTTLPIYMMKTSILEGNYGY 272 +I MTNGGP D T + Y+ + ++GY Sbjct: 249 LIIVMTNGGPADRTQIVASYIFTQAFKRLDFGY 281 >ref|NP_357428.1| AGR_L_3272p [Agrobacterium tumefaciens] ref|NP_533673.1| ABC transporter, membrane spanning protein [sugar] [Agrobacterium tumefaciens str. C58 (U. Washington)] pir||C98336 probable integral membrane transport protein (L41665) [imported] - Agrobacterium tumefaciens (strain C58, Cereon) pir||AG2946 hypothetical protein Atu3173 [imported] - Agrobacterium tumefaciens (strain C58, Dupont) gb|AAK90213.1| AGR_L_3272p [Agrobacterium tumefaciens str. C58 (Cereon)] gb|AAL43989.1| ABC transporter, membrane spanning protein [sugar] [Agrobacterium tumefaciens str. C58 (U. Washington)] Length = 337 Score = 177 bits (450), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 104/281 (37%), Positives = 149/281 (53%), Gaps = 10/281 (3%) Query: 4 TRQRKRKKG----EAWI-PYLLIAPTVLLIAGILIFPIFRVFDLSVQSYDFTRLQD--AG 56 R R RK W P+L AP ++LI +++ P+ V LS D L G Sbjct: 35 ARNRARKPAMRRFADWAEPWLYSAPVLVLIVAVMLVPL--VLGLSYAFRDVQLLNPFPGG 92 Query: 57 YIGLENFRHIFTEDDLFYSTLVTTLKWVFSEVALQLVFGLIVALLLNQSFXXXXXXXXXX 116 ++GL++ + ++D FY L TL W + V LQ FGLI+ALLL++ F Sbjct: 93 FVGLKHLEAL-SQDAAFYRALKNTLWWTGASVFLQFFFGLILALLLDKPFAGRGLAQALV 151 Query: 117 XXPWAVSGVLTTMLWSLMFNQHIGIINDILLKLGIIHEKIAWLANPDTVFGSVVFAELWR 176 PWAV L + W+ +FN IG + ++ LG++ LA+P + A +W Sbjct: 152 FLPWAVPTFLAGLNWAWLFNPVIGPLPHWMVSLGLLSAPNNILADPQLAMWGPIIANVWW 211 Query: 177 GIPFFAITLLAALQTIPHEVYESCEVDGAGKLKKLFHITLPYLKESIIFATLLRAIWEFN 236 GIPFFAITLLAALQ IP ++YE+ E+DGA ++ ITLP+L +I LLR +W N Sbjct: 212 GIPFFAITLLAALQAIPRDLYEAAEIDGANPFQRFTSITLPFLAPTIAITVLLRTVWIAN 271 Query: 237 SIDMIFTMTNGGPMDMTTTLPIYMMKTSILEGNYGYGSALG 277 D+I MTNGGP D T + Y+ + ++GY SA+ Sbjct: 272 FADLIIVMTNGGPADRTQIVASYIFTQAFRRLDFGYASAIA 312 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.329 0.144 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 423,252,788 Number of extensions: 16103642 Number of successful extensions: 57572 Number of sequences better than 1.0e-05: 1412 Number of HSP's gapped: 56157 Number of HSP's successfully gapped: 1414 Length of query: 303 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 178 Effective length of database: 417,850,203 Effective search space: 74377336134 Effective search space used: 74377336134 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 125 (52.8 bits)