BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0011.p (407 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_244451.1| ATP phosphoribosyltransferase regulatory subuni... 286 6e-76 ref|NP_391373.1| histidyl-tRNA synthetase [Bacillus subtilis] >g... 264 3e-69 ref|NP_954347.1| histidyl-tRNA synthetase, putative [Geobacter s... 216 6e-55 ref|ZP_00103827.1| COG3705: ATP phosphoribosyltransferase involv... 202 9e-51 ref|ZP_00082172.1| COG3705: ATP phosphoribosyltransferase involv... 200 4e-50 >ref|NP_244451.1| ATP phosphoribosyltransferase regulatory subunit/histidyl-tRNA synthetase; histidyl-tRNA synthetase [Bacillus halodurans] sp|Q9K6Z0|HISZ_BACHD ATP phosphoribosyltransferase regulatory subunit pir||H84097 histidyl-tRNA synthetase hisZ [imported] - Bacillus halodurans (strain C-125) dbj|BAB07303.1| ATP phosphoribosyltransferase regulatory subunit/histidyl-tRNA synthetase [Bacillus halodurans] Length = 397 Score = 286 bits (732), Expect = 6e-76, Method: Compositional matrix adjust. Identities = 144/327 (44%), Positives = 208/327 (63%), Gaps = 1/327 (0%) Query: 3 MSKPKGFEKPAGVRDYLPRAVTKLRKIEKDVLHCMSRWGYRQMITPTLEYYDTVGVASST 62 MSKP FEKP G+RD LP + I + ++ WGY + TPTLEYY+TVGV S+ Sbjct: 1 MSKPFMFEKPFGMRDTLPEWYKTKKNICDQMTEEINLWGYDMIETPTLEYYETVGVVSAI 60 Query: 63 SDQKLYKLLNNRGQALVLRSEMTAPVARVVSSLLKDEPLPLRLSYHANVXXXXXXXXXXX 122 DQ+L+KLL+ +G LVLR +MTAP+AR+V+S LKD PLRL+Y +NV Sbjct: 61 LDQQLFKLLDQQGNTLVLRPDMTAPIARLVASSLKDRAYPLRLAYQSNVYRAQQNEGGKP 120 Query: 123 XXXXQTGVELVGDDSPEADAEVVALAISSLQAAGVKSFKIAMGHVGFLDGLFQEAVAGLP 182 Q GVEL+GD + AD EV+AL I++L+ AG+ FK+A+GHVG+++ L + V G Sbjct: 121 AEFEQLGVELIGDGTASADGEVIALMIAALKRAGLSEFKVAIGHVGYVNALLMD-VVGNE 179 Query: 183 DAQEELKSHLLNRDYVAFRETLRRLELSEAQKNELDGLLRLRGGKEICGQALELSSHPLA 242 + L+ L ++YV +RE ++ L LS K+ L LL LRGG+ +A L Sbjct: 180 QRADRLRRFLYEKNYVGYREHVKSLNLSTIDKSRLMNLLSLRGGRAAIEEARGLIQTEKG 239 Query: 243 RASIEHLCKVWEVLVSYGVSQHVLIDLTMIGDFSYYTGMTFEGYASELGFPVCSGGRYDN 302 + ++ + K++EVL SYG S++V DLT++ SYYTG+ FEGY + LG P+CSGGRYD Sbjct: 240 KTALAEMTKLYEVLESYGASEYVKFDLTLVLHMSYYTGVVFEGYGNRLGVPLCSGGRYDE 299 Query: 303 LLQQFGRPVPSTGFSLKTNRILDGVSG 329 LL +F RP +TGF ++ + +++ ++G Sbjct: 300 LLSKFHRPAQATGFGVRIDLLVEALNG 326 >ref|NP_391373.1| histidyl-tRNA synthetase [Bacillus subtilis] sp|O34459|HISZ_BACSU ATP phosphoribosyltransferase regulatory subunit pir||H69641 histidine-tRNA ligase (EC 6.1.1.21) hisZ - Bacillus subtilis gb|AAC67293.1| histidyl-tRNA synthetase [Bacillus subtilis] emb|CAB15498.1| histidyl-tRNA synthetase [Bacillus subtilis subsp. subtilis str. 168] Length = 391 Score = 264 bits (674), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 141/332 (42%), Positives = 206/332 (62%), Gaps = 1/332 (0%) Query: 9 FEKPAGVRDYLPRAVTKLRKIEKDVLHCMSRWGYRQMITPTLEYYDTVGVASSTSDQKLY 68 FEKP G+RD LP +K+ + + + +WGY+ M TPTLE+YDTVGV S+ +Q+L+ Sbjct: 4 FEKPHGMRDTLPGLYETKKKVRRSLTDLIDKWGYQFMETPTLEFYDTVGVQSAIEEQQLF 63 Query: 69 KLLNNRGQALVLRSEMTAPVARVVSSLLKDEPLPLRLSYHANVXXXXXXXXXXXXXXXQT 128 KLL+ G+ LVLR +MT P+ARV +S L PLR+ Y ANV Q Sbjct: 64 KLLDQDGKTLVLRPDMTGPIARVAASKLLKHGHPLRVGYAANVFRAQEREGGRPAEFEQV 123 Query: 129 GVELVGDDSPEADAEVVALAISSLQAAGVKSFKIAMGHVGFLDGLFQEAVAGLPDAQEEL 188 GVEL+GD + ADAEV+AL + +L+ AG+ SFKIA+GH G D LF E + + A + L Sbjct: 124 GVELIGDGTTSADAEVIALVVGALKNAGLASFKIAIGHAGIADALFVEVLGNVERA-DVL 182 Query: 189 KSHLLNRDYVAFRETLRRLELSEAQKNELDGLLRLRGGKEICGQALELSSHPLARASIEH 248 + L ++YV +RE ++ L LS K+ L LL LRGG E+CG+A E+ ++ ++ Sbjct: 183 RRFLYEKNYVGYREHVKSLPLSSIDKSRLLELLELRGGIEVCGRAEEIVDSAQGKSVVDE 242 Query: 249 LCKVWEVLVSYGVSQHVLIDLTMIGDFSYYTGMTFEGYASELGFPVCSGGRYDNLLQQFG 308 L +W++L YG +++V +DL M+ SYYTG+ FE YA +GF + SGGRY+ LL F Sbjct: 243 LKALWDILEDYGCTENVRLDLNMVSHMSYYTGILFEVYAENVGFVIGSGGRYNKLLGHFD 302 Query: 309 RPVPSTGFSLKTNRILDGVSGVPEEEELPILI 340 P P+TGF L+ +R+++ + E E+ +I Sbjct: 303 SPAPATGFGLRIDRLIEALHMKDEPCEIDAVI 334 >ref|NP_954347.1| histidyl-tRNA synthetase, putative [Geobacter sulfurreducens PCA] gb|AAR36697.1| histidyl-tRNA synthetase, putative [Geobacter sulfurreducens PCA] Length = 438 Score = 216 bits (551), Expect = 6e-55, Method: Compositional matrix adjust. Identities = 116/326 (35%), Positives = 187/326 (57%), Gaps = 5/326 (1%) Query: 12 PAGVRDYLPRAVTKLRKIEKDVLHCMSRWGYRQMITPTLEYYDTVGVASSTSDQKLYKLL 71 P GV D+LP K+ IE + WG+R++ITP LE+ D + + Sbjct: 12 PKGVTDFLPEKADKIGYIEGKIRKVFELWGFRRIITPLLEFEDVIAAGLGDDLKAKTFRF 71 Query: 72 NNR--GQALVLRSEMTAPVARVVSSLLKDEPLPLRLSYHANVXXXXXXXXXXXXXXXQTG 129 ++R G+ + + S++T +AR+V++ L+ PLP R+ Y V Q+G Sbjct: 72 DDRQSGKLIAVPSDITPQIARIVATRLRGYPLPHRICYSGRVLRHAELQSGRSREIFQSG 131 Query: 130 VELVGDDSPEADAEVVALAISSLQAAGVKSFKIAMGHVGFLDGLFQEAVAGLPDA-QEEL 188 VEL+G DSPEADAE+V +A+ +L+ G + FKI +GHVGF+ G+ +GL A + L Sbjct: 132 VELIGLDSPEADAEMVTMAVEALKGLGFRDFKIDLGHVGFIRGIM--TASGLEVAVRNRL 189 Query: 189 KSHLLNRDYVAFRETLRRLELSEAQKNELDGLLRLRGGKEICGQALELSSHPLARASIEH 248 + + +D A R L LS+A K+EL L RL GG+E+ +A ++++ +R ++++ Sbjct: 190 QEAIGKKDVSAVRSILAESPLSDAAKDELAALPRLFGGREVLDEAGRVATNDTSRRALDN 249 Query: 249 LCKVWEVLVSYGVSQHVLIDLTMIGDFSYYTGMTFEGYASELGFPVCSGGRYDNLLQQFG 308 + +V ++L +GVS H+ IDL + Y+TG+TFEG+ + +G VCSGGRYD L ++G Sbjct: 250 ISQVLDLLDIHGVSDHLTIDLGEVRGLDYHTGLTFEGFVTGMGEAVCSGGRYDTLTARYG 309 Query: 309 RPVPSTGFSLKTNRILDGVSGVPEEE 334 P P+TGF+ +L + P+ E Sbjct: 310 FPAPATGFTFNVLALLSALEKRPDVE 335 >ref|ZP_00103827.1| COG3705: ATP phosphoribosyltransferase involved in histidine biosynthesis [Desulfitobacterium hafniense] Length = 383 Score = 202 bits (515), Expect = 9e-51, Method: Compositional matrix adjust. Identities = 116/322 (36%), Positives = 171/322 (53%), Gaps = 7/322 (2%) Query: 8 GFEKPAGVRDYLPRAVTKLRKIEKDVLHCMSRWGYRQMITPTLEYYDTVGVASSTSDQKL 67 G P G+ D LP + + E L W Y++++TPTLEY + D Sbjct: 7 GLRIPEGMHDLLPDELALQERAEASALDLFKAWAYQKVVTPTLEYGACIQPVEEEED-SF 65 Query: 68 YKLLNNRGQALVLRSEMTAPVARVVSSLLKDEPLPLRLSYHANVXXXXXXXXXXXXXXXQ 127 +KL + +G LVLR E+T P+AR+VS+ ++ PLRL Y A+V Q Sbjct: 66 FKLFDRQGHVLVLRPELTTPIARMVSTRMRGTTFPLRLCYAADVFRYSKSHKQEFR---Q 122 Query: 128 TGVELVGDDSPEADAEVVALAISSLQAAGVKSFKIAMGHVGFLDGLFQEAVAGLP-DAQE 186 GVEL+G SP ADAEVVALAI +L+ G F+I +GH+G G+ E G+P + Q Sbjct: 123 VGVELIGSASPAADAEVVALAIEALRQIGGMDFQINLGHMGIFTGIMAEL--GVPQEFQL 180 Query: 187 ELKSHLLNRDYVAFRETLRRLELSEAQKNELDGLLRLRGGKEICGQALELSSHPLARASI 246 + L +D+V ++ ++ L L L G +++ Q LE S P ++ Sbjct: 181 HYQEKLARKDFVGIERLVKDYGFELKVQDVLLKLPHLHGQEDMLDQVLEWSRRPSLLEAV 240 Query: 247 EHLCKVWEVLVSYGVSQHVLIDLTMIGDFSYYTGMTFEGYASELGFPVCSGGRYDNLLQQ 306 L +V+ L +GV +V +DL ++ FSYYTG FEGY +GFPV GGRYD L + Sbjct: 241 AALRQVYRYLKDFGVQDYVSLDLGILRGFSYYTGAVFEGYVPGVGFPVVEGGRYDALYGE 300 Query: 307 FGRPVPSTGFSLKTNRILDGVS 328 FG VP+TGF++ I++ ++ Sbjct: 301 FGEDVPATGFAINLKAIIEQMA 322 >ref|ZP_00082172.1| COG3705: ATP phosphoribosyltransferase involved in histidine biosynthesis [Geobacter metallireducens] Length = 399 Score = 200 bits (509), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 108/298 (36%), Positives = 176/298 (59%), Gaps = 5/298 (1%) Query: 40 WGYRQMITPTLEYYDTVGVASSTSDQKLYKLLNNR--GQALVLRSEMTAPVARVVSSLLK 97 WG+R++ITP LE+ D + ++ ++R G+ L + S++T VAR+V++ ++ Sbjct: 5 WGFRRIITPLLEFQDVMAAGLGEDLKERTFRFDDRQTGKLLAIPSDITPQVARIVATRMR 64 Query: 98 DEPLPLRLSYHANVXXXXXXXXXXXXXXXQTGVELVGDDSPEADAEVVALAISSLQAAGV 157 PLP RL Y V Q GVEL+G DSPEADAE+VA+A+ L+ G Sbjct: 65 GCPLPHRLYYIGRVLRHVELQSGRSRETFQAGVELIGLDSPEADAEMVAMAVEILKGLGF 124 Query: 158 KSFKIAMGHVGFLDGLFQEAVAGLP-DAQEELKSHLLNRDYVAFRETLRRLELSEAQKNE 216 + FK+ +GH GF+ G+ A +GL DA+ L+ + +D A R L +++ K E Sbjct: 125 EEFKVDLGHTGFIRGVM--AASGLGGDARRRLQEAVGKKDSSAVRAILETEPVADRIKEE 182 Query: 217 LDGLLRLRGGKEICGQALELSSHPLARASIEHLCKVWEVLVSYGVSQHVLIDLTMIGDFS 276 L L RL GG+E+ +A +++ +R +++++ +V ++L +GVS H+ +DL I Sbjct: 183 LAALPRLFGGREVLAEAARVATSDSSRRALDNIAQVLDILDIHGVSDHLTLDLGEIRGLD 242 Query: 277 YYTGMTFEGYASELGFPVCSGGRYDNLLQQFGRPVPSTGFSLKTNRILDGVSGVPEEE 334 Y++G+TFEG+ + +G VCSGGRYDNL Q++G P P+TGF+ +L+ + P+ E Sbjct: 243 YHSGLTFEGFVTGIGEAVCSGGRYDNLTQRYGYPAPATGFAFNILALLNALEKRPDVE 300 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.136 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 538,256,245 Number of extensions: 20423214 Number of successful extensions: 47651 Number of sequences better than 1.0e-05: 445 Number of HSP's gapped: 47265 Number of HSP's successfully gapped: 445 Length of query: 407 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 279 Effective length of database: 409,704,906 Effective search space: 114307668774 Effective search space used: 114307668774 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)