BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0004.p (217 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_693400.1| phosphoglycolate phosphatase [Oceanobacillus ih... 169 5e-41 ref|NP_653608.1| Hydrolase, haloacid dehalogenase-like hydrolase... 163 3e-39 ref|NP_981558.1| hydrolase, haloacid dehalogenase-like family [B... 163 3e-39 ref|NP_834826.1| Phosphoglycolate phosphatase [Bacillus cereus A... 162 4e-39 ref|NP_391377.1| similar to phosphoglycolate phosphatase [Bacill... 155 6e-37 >ref|NP_693400.1| phosphoglycolate phosphatase [Oceanobacillus iheyensis HTE831] dbj|BAC14435.1| phosphoglycolate phosphatase [Oceanobacillus iheyensis HTE831] Length = 214 Score = 169 bits (427), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 92/211 (43%), Positives = 125/211 (59%), Gaps = 6/211 (2%) Query: 2 IECILFDLDGTIVNTNELIINSFMHTLKENNLPPLTREQIIPYMGTTLHQQLSAFSGLED 61 I ILFDLDGT+++TN LI SF HT KE NL + E+I+ + G L + F+ +++ Sbjct: 3 IRTILFDLDGTLIDTNTLIKASFEHTFKEYNLN-FSNEEILKFNGPPL---VDTFNKIDE 58 Query: 62 TSALEL--SYRSYNYAHHDELIGSFPHVNEIMEELAGRGIKLGIVTTKIRPTTIKALEMF 119 T A + ++R +N HD + +FPHV + +EEL R I LGIV+TK+R T LE+ Sbjct: 59 TKADRMITTFREHNIREHDNFVTAFPHVYDTLEELQNRNISLGIVSTKMRHTVHMGLELT 118 Query: 120 DLLKYMETIVTVNDVVQPKPHAEPVLKAVSNLGVDPRRTLMVGDSPVDIQSXXXXXXXXX 179 + K+ TI+T +DV KPH EPV A+ LG P TLMVGD+ DI S Sbjct: 119 GISKFFSTIITYDDVTHAKPHPEPVQMAMQKLGAHPEHTLMVGDNHHDIVSGQRANVQTA 178 Query: 180 XXXWSLKGEDVLRKYEPDYIIHDMKDIFGIV 210 WSLK + L+ + PDYII D+KDI IV Sbjct: 179 AVAWSLKDTNYLKSFHPDYIIEDIKDIITIV 209 >ref|NP_653608.1| Hydrolase, haloacid dehalogenase-like hydrolase [Bacillus anthracis A2012] ref|NP_847563.1| hydrolase, haloacid dehalogenase-like family [Bacillus anthracis str. Ames] gb|AAP29049.1| hydrolase, haloacid dehalogenase-like family [Bacillus anthracis str. Ames] Length = 216 Score = 163 bits (412), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 86/211 (40%), Positives = 126/211 (59%), Gaps = 5/211 (2%) Query: 2 IECILFDLDGTIVNTNELIINSFMHTLKENNLPPLTREQIIPYMGTTLHQQLSAFSGLED 61 I +LFDLDGT++NTNELII+SF+HTL RE ++P++G +LH FS +++ Sbjct: 3 INTVLFDLDGTLINTNELIISSFLHTLHTYYPNQYKREDVLPFIGPSLH---DTFSKIDE 59 Query: 62 TSALEL--SYRSYNYAHHDELIGSFPHVNEIMEELAGRGIKLGIVTTKIRPTTIKALEMF 119 + EL SYR +N+ HHDEL+ + V E ++EL +G K+GIVTTK R T L++ Sbjct: 60 SKVEELITSYRQFNHDHHDELVEEYETVYETVQELKKQGYKVGIVTTKARQTVEMGLKLS 119 Query: 120 DLLKYMETIVTVNDVVQPKPHAEPVLKAVSNLGVDPRRTLMVGDSPVDIQSXXXXXXXXX 179 L ++ + +VT++DV KPH EP+ KA+ L P LMVGD+ DI Sbjct: 120 KLDEFFDVVVTIDDVEHVKPHPEPLQKALQLLDAKPEEALMVGDNHHDIVGGQNAGTKTA 179 Query: 180 XXXWSLKGEDVLRKYEPDYIIHDMKDIFGIV 210 W+LKG L Y+PD+++ M D+ I+ Sbjct: 180 AVSWTLKGRAYLETYKPDFMLDKMSDLLLIL 210 >ref|NP_981558.1| hydrolase, haloacid dehalogenase-like family [Bacillus cereus ATCC 10987] gb|AAS44166.1| hydrolase, haloacid dehalogenase-like family [Bacillus cereus ATCC 10987] Length = 216 Score = 163 bits (412), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 86/211 (40%), Positives = 125/211 (59%), Gaps = 5/211 (2%) Query: 2 IECILFDLDGTIVNTNELIINSFMHTLKENNLPPLTREQIIPYMGTTLHQQLSAFSGLED 61 I +LFDLDGT++NTNELII+SF+HTL RE ++P++G +LH FS +++ Sbjct: 3 INTVLFDLDGTLINTNELIISSFLHTLHTYYPNQYKREDVLPFIGPSLH---DTFSKIDE 59 Query: 62 TSALEL--SYRSYNYAHHDELIGSFPHVNEIMEELAGRGIKLGIVTTKIRPTTIKALEMF 119 + EL SYR +N+ HHDEL+ + V E ++EL +G K+GIVTTK R T L+ Sbjct: 60 SKVEELITSYRQFNHDHHDELVEEYETVYETVQELKKQGYKVGIVTTKARQTVEMGLKFS 119 Query: 120 DLLKYMETIVTVNDVVQPKPHAEPVLKAVSNLGVDPRRTLMVGDSPVDIQSXXXXXXXXX 179 L ++ + +VT++DV KPH EP+ KA+ L P LMVGD+ DI Sbjct: 120 KLDEFFDVVVTIDDVEHVKPHPEPLQKALQLLDAKPEEALMVGDNHHDIVGGQNAGTKTA 179 Query: 180 XXXWSLKGEDVLRKYEPDYIIHDMKDIFGIV 210 W+LKG L Y+PD+++ M D+ I+ Sbjct: 180 AVSWTLKGRAYLEAYKPDFMLDKMSDLLPIL 210 >ref|NP_834826.1| Phosphoglycolate phosphatase [Bacillus cereus ATCC 14579] gb|AAP12027.1| Phosphoglycolate phosphatase [Bacillus cereus ATCC 14579] Length = 216 Score = 162 bits (411), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 85/211 (40%), Positives = 125/211 (59%), Gaps = 5/211 (2%) Query: 2 IECILFDLDGTIVNTNELIINSFMHTLKENNLPPLTREQIIPYMGTTLHQQLSAFSGLED 61 I +LFDLDGT++NTNELII+SF+HTL RE ++P++G +LH FS +++ Sbjct: 3 INTVLFDLDGTLINTNELIISSFLHTLNTYYPNQYKREDVLPFIGPSLH---DTFSKIDE 59 Query: 62 TSALEL--SYRSYNYAHHDELIGSFPHVNEIMEELAGRGIKLGIVTTKIRPTTIKALEMF 119 + E+ SYR +N+ HHDEL+ + V E + EL +G K+GIVTTK R T L++ Sbjct: 60 SKVEEMITSYREFNHDHHDELVEEYETVYETVRELKKQGYKVGIVTTKARQTVEMGLQLS 119 Query: 120 DLLKYMETIVTVNDVVQPKPHAEPVLKAVSNLGVDPRRTLMVGDSPVDIQSXXXXXXXXX 179 L ++ + +VT++DV KPH EP+ KA+ L P LMVGD+ DI Sbjct: 120 KLDEFFDVVVTIDDVEHVKPHPEPLQKALELLDAKPEEALMVGDNHHDIVGGQNAGTKTA 179 Query: 180 XXXWSLKGEDVLRKYEPDYIIHDMKDIFGIV 210 W+LKG L Y+PD+++ M D+ I+ Sbjct: 180 AVSWTLKGRAYLEAYKPDFMLDKMSDLLPIL 210 >ref|NP_391377.1| similar to phosphoglycolate phosphatase [Bacillus subtilis] sp|Q9JMQ2|HPRP_BACSU HPr(Ser) phosphatase (P-Ser-HPr phosphatase) pir||G70044 histidine-containing protein P-Ser-HPr phosphatase (EC 3.1.3.-) [validated] - Bacillus subtilis gb|AAC67289.1| HPr(Ser-P) phosphatase [Bacillus subtilis] emb|CAB15502.1| P-Ser-HPr phosphatase [Bacillus subtilis subsp. subtilis str. 168] Length = 216 Score = 155 bits (392), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 82/211 (38%), Positives = 124/211 (58%), Gaps = 5/211 (2%) Query: 2 IECILFDLDGTIVNTNELIINSFMHTLKENNLPPLTREQIIPYMGTTLHQQLSAFS--GL 59 + ILFDLDGT++NTNELII SF+HTL+ RE ++ ++G +L S+ Sbjct: 6 VTTILFDLDGTLINTNELIIASFLHTLEHYYPSKYKREDVLAFIGPSLFDTFSSMDPDKC 65 Query: 60 EDTSALELSYRSYNYAHHDELIGSFPHVNEIMEELAGRGIKLGIVTTKIRPTTIKALEMF 119 ED A+ YR+YN+ HD L+ + V E ++ L G LGIVTTK+R T L++ Sbjct: 66 EDMIAM---YRAYNHDMHDSLVTEYETVYETLDALKKAGFTLGIVTTKLRDTVNMGLKLT 122 Query: 120 DLLKYMETIVTVNDVVQPKPHAEPVLKAVSNLGVDPRRTLMVGDSPVDIQSXXXXXXXXX 179 + ++ ET+VT++DV KP EPVL A+ LG +P +MVGD+ D+ + Sbjct: 123 GIGEFFETVVTLDDVTNAKPDPEPVLLALKQLGSEPAEAIMVGDNYHDVLAGKNAGTKTA 182 Query: 180 XXXWSLKGEDVLRKYEPDYIIHDMKDIFGIV 210 W++KG ++L K+EPD+++ M D+ IV Sbjct: 183 GVAWTIKGPEMLAKHEPDFMLEKMSDLLQIV 213 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.138 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 304,110,281 Number of extensions: 11427378 Number of successful extensions: 31823 Number of sequences better than 1.0e-05: 328 Number of HSP's gapped: 31770 Number of HSP's successfully gapped: 328 Length of query: 217 Length of database: 757,237,578 Length adjustment: 121 Effective length of query: 96 Effective length of database: 428,710,599 Effective search space: 41156217504 Effective search space used: 41156217504 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 123 (52.0 bits)