BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0002.p (312 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_391380.1| similar to hypothetical proteins [Bacillus subt... 439 e-122 ref|NP_834828.1| HPR(SER) kinase [Bacillus cereus ATCC 14579] >g... 431 e-120 ref|NP_981560.1| HPr(Ser) kinase/phosphatase [Bacillus cereus AT... 430 e-119 ref|NP_653610.1| Hpr_kinase, Hpr S kinase [Bacillus anthracis A2... 430 e-119 ref|NP_244457.1| HPr (Ser/Thr) protein kinase/phosphatase [Bacil... 391 e-108 >ref|NP_391380.1| similar to hypothetical proteins [Bacillus subtilis] sp|O34483|HPRK_BACSU HPr(Ser) kinase/phosphatase pir||E70044 Hpr kinase (EC 2.7.1.-) [validated] - Bacillus subtilis gb|AAC67286.1| HPr(Ser) kinase [Bacillus subtilis] emb|CAB15505.1| serine/threonine protein kinase [Bacillus subtilis subsp. subtilis str. 168] Length = 310 Score = 439 bits (1130), Expect = e-122, Method: Compositional matrix adjust. Identities = 206/303 (67%), Positives = 252/303 (83%) Query: 4 KVKVSELVQNFQLEVVSGHEGLKRPITVDDLNRPGLEMAGYFEYYPEERVQLLGKTELAF 63 KV+ ++++ F LE++SG EG+ RPIT+ DL+RPG+E+AGYF YYP ERVQLLGKTEL+F Sbjct: 3 KVRTKDVMEQFNLELISGEEGINRPITMSDLSRPGIEIAGYFTYYPRERVQLLGKTELSF 62 Query: 64 FSMLPDEERKSRIRGICNDNTPCIVITRALDVPQELIDISNEKGLPVLRSSMATTIFSSR 123 F LP+EE+K R+ +C D TP I+++R + +PQELID S + G+PVLRS + TT SSR Sbjct: 63 FEQLPEEEKKQRMDSLCTDVTPAIILSRDMPIPQELIDASEKNGVPVLRSPLKTTRLSSR 122 Query: 124 LTSFLEGRLAPTATIHGVLCDVYGVGMLITGSSGIGKSETALELVKRGHRLIADDAVEIR 183 LT+FLE RLAPT IHGVL D+YGVG+LITG SG+GKSETALELVKRGHRL+ADD VEIR Sbjct: 123 LTNFLESRLAPTTAIHGVLVDIYGVGVLITGKSGVGKSETALELVKRGHRLVADDCVEIR 182 Query: 184 QTSDNQLHGTAPELIRHLLEIRGVGIINVMTLFGAGAIRNHKRITLVVRLEAWQQDKQYD 243 Q + L G APELI HLLEIRG+GIINVMTLFGAGA+R++KRIT+V+ LE W+Q KQYD Sbjct: 183 QEDQDTLVGNAPELIEHLLEIRGLGIINVMTLFGAGAVRSNKRITIVMNLELWEQGKQYD 242 Query: 244 RLGLDEETTRIIDTDVPLVTIPVRPGRNLAVIIEVAAMNYRLKQMGFNAALQFTNKLTAT 303 RLGL+EET +IIDT++ +TIPVRPGRNLAVIIEVAAMN+RLK+MG NAA QFTNKL Sbjct: 243 RLGLEEETMKIIDTEITKLTIPVRPGRNLAVIIEVAAMNFRLKRMGLNAAEQFTNKLADV 302 Query: 304 ISE 306 I + Sbjct: 303 IED 305 >ref|NP_834828.1| HPR(SER) kinase [Bacillus cereus ATCC 14579] gb|AAP12029.1| HPR(SER) kinase [Bacillus cereus ATCC 14579] Length = 309 Score = 431 bits (1109), Expect = e-120, Method: Compositional matrix adjust. Identities = 197/301 (65%), Positives = 248/301 (82%) Query: 4 KVKVSELVQNFQLEVVSGHEGLKRPITVDDLNRPGLEMAGYFEYYPEERVQLLGKTELAF 63 KV+ +L++ FQLE++SG EG+ RPI DL+RPG+EMAG+F YYP +RVQLLGKTEL F Sbjct: 3 KVRTKDLIEQFQLELISGEEGIHRPIDTSDLSRPGIEMAGFFTYYPADRVQLLGKTELTF 62 Query: 64 FSMLPDEERKSRIRGICNDNTPCIVITRALDVPQELIDISNEKGLPVLRSSMATTIFSSR 123 F L E+++ R++ +C + TPCI++TR DVP EL+ S E G+P+LRSS TT SSR Sbjct: 63 FDTLTSEQKQERMKALCTEETPCIIVTRNQDVPDELLQASRESGMPLLRSSQTTTRLSSR 122 Query: 124 LTSFLEGRLAPTATIHGVLCDVYGVGMLITGSSGIGKSETALELVKRGHRLIADDAVEIR 183 LT++LEG+LAPT +HGVL D+YGVG+LITG SG+GKSETALELVKRGHRL+ADD+VEIR Sbjct: 123 LTNYLEGKLAPTTAVHGVLVDIYGVGVLITGQSGVGKSETALELVKRGHRLVADDSVEIR 182 Query: 184 QTSDNQLHGTAPELIRHLLEIRGVGIINVMTLFGAGAIRNHKRITLVVRLEAWQQDKQYD 243 Q ++ L G++P+LI HLLEIRG+GIINVMTLFGAGA+RN+KRITLV+ LE W Q K YD Sbjct: 183 QEDEDMLVGSSPDLIEHLLEIRGLGIINVMTLFGAGAVRNYKRITLVINLEIWDQKKNYD 242 Query: 244 RLGLDEETTRIIDTDVPLVTIPVRPGRNLAVIIEVAAMNYRLKQMGFNAALQFTNKLTAT 303 RLGLDEE +IIDT++ +T+PVRPGRNLAVIIEVAAMN+RLK+MG NAA QF+ +L + Sbjct: 243 RLGLDEEKMKIIDTELTKITLPVRPGRNLAVIIEVAAMNFRLKRMGVNAAQQFSERLMSA 302 Query: 304 I 304 I Sbjct: 303 I 303 >ref|NP_981560.1| HPr(Ser) kinase/phosphatase [Bacillus cereus ATCC 10987] gb|AAS44168.1| HPr(Ser) kinase/phosphatase [Bacillus cereus ATCC 10987] Length = 352 Score = 430 bits (1106), Expect = e-119, Method: Compositional matrix adjust. Identities = 197/301 (65%), Positives = 248/301 (82%) Query: 4 KVKVSELVQNFQLEVVSGHEGLKRPITVDDLNRPGLEMAGYFEYYPEERVQLLGKTELAF 63 KV+ +L++ FQLE++SG EG+ RPI DL+RPG+EMAG+F YYP +RVQLLGKTEL F Sbjct: 46 KVRTKDLIEQFQLELISGEEGIHRPIDTSDLSRPGIEMAGFFTYYPADRVQLLGKTELTF 105 Query: 64 FSMLPDEERKSRIRGICNDNTPCIVITRALDVPQELIDISNEKGLPVLRSSMATTIFSSR 123 F L E+++ R++ +C + TPCI++TR DVP EL+ S E G+P+LRSS TT SSR Sbjct: 106 FDTLTSEQKQERMKALCTEETPCIIVTRNQDVPDELLQASRESGMPLLRSSQTTTRLSSR 165 Query: 124 LTSFLEGRLAPTATIHGVLCDVYGVGMLITGSSGIGKSETALELVKRGHRLIADDAVEIR 183 LT++LEG+LAPT +HGVL D+YGVG+LITG SG+GKSETALELVKRGHRL+ADD+VEIR Sbjct: 166 LTNYLEGKLAPTTAVHGVLVDIYGVGVLITGQSGVGKSETALELVKRGHRLVADDSVEIR 225 Query: 184 QTSDNQLHGTAPELIRHLLEIRGVGIINVMTLFGAGAIRNHKRITLVVRLEAWQQDKQYD 243 Q ++ L G++P+LI HLLEIRG+GIINVMTLFGAGA+RN+KRITLV+ LE W Q K YD Sbjct: 226 QEDEDMLVGSSPDLIEHLLEIRGLGIINVMTLFGAGAVRNYKRITLVINLEIWDQKKNYD 285 Query: 244 RLGLDEETTRIIDTDVPLVTIPVRPGRNLAVIIEVAAMNYRLKQMGFNAALQFTNKLTAT 303 RLGLDEE +IIDT++ +T+PVRPGRNLAVIIEVAAMN+RLK+MG NAA QF+ +L + Sbjct: 286 RLGLDEEKMKIIDTELTKITLPVRPGRNLAVIIEVAAMNFRLKRMGVNAAQQFSERLMSA 345 Query: 304 I 304 I Sbjct: 346 I 346 >ref|NP_653610.1| Hpr_kinase, Hpr S kinase [Bacillus anthracis A2012] ref|NP_847565.1| HPr(Ser) kinase/phosphatase [Bacillus anthracis str. Ames] gb|AAP29051.1| HPr(Ser) kinase/phosphatase [Bacillus anthracis str. Ames] Length = 309 Score = 430 bits (1105), Expect = e-119, Method: Compositional matrix adjust. Identities = 196/301 (65%), Positives = 248/301 (82%) Query: 4 KVKVSELVQNFQLEVVSGHEGLKRPITVDDLNRPGLEMAGYFEYYPEERVQLLGKTELAF 63 KV+ +L++ FQLE++SG EG+ RPI DL+RPG+EMAG+F YYP +RVQLLGKTEL F Sbjct: 3 KVRTKDLIEQFQLELISGEEGIHRPIDTSDLSRPGIEMAGFFTYYPADRVQLLGKTELTF 62 Query: 64 FSMLPDEERKSRIRGICNDNTPCIVITRALDVPQELIDISNEKGLPVLRSSMATTIFSSR 123 F L ++++ R++ +C + TPCI++TR DVP EL+ S E G+P+LRSS TT SSR Sbjct: 63 FDTLTSDQKQERMKALCTEETPCIIVTRNQDVPDELLQASRESGMPLLRSSQTTTRLSSR 122 Query: 124 LTSFLEGRLAPTATIHGVLCDVYGVGMLITGSSGIGKSETALELVKRGHRLIADDAVEIR 183 LT++LEG+LAPT +HGVL D+YGVG+LITG SG+GKSETALELVKRGHRL+ADD+VEIR Sbjct: 123 LTNYLEGKLAPTTAVHGVLVDIYGVGVLITGQSGVGKSETALELVKRGHRLVADDSVEIR 182 Query: 184 QTSDNQLHGTAPELIRHLLEIRGVGIINVMTLFGAGAIRNHKRITLVVRLEAWQQDKQYD 243 Q ++ L G++P+LI HLLEIRG+GIINVMTLFGAGA+RN+KRITLV+ LE W Q K YD Sbjct: 183 QEDEDMLVGSSPDLIEHLLEIRGLGIINVMTLFGAGAVRNYKRITLVINLEIWDQKKNYD 242 Query: 244 RLGLDEETTRIIDTDVPLVTIPVRPGRNLAVIIEVAAMNYRLKQMGFNAALQFTNKLTAT 303 RLGLDEE +IIDT++ +T+PVRPGRNLAVIIEVAAMN+RLK+MG NAA QF+ +L + Sbjct: 243 RLGLDEEKMKIIDTELTKITLPVRPGRNLAVIIEVAAMNFRLKRMGVNAAQQFSERLMSA 302 Query: 304 I 304 I Sbjct: 303 I 303 >ref|NP_244457.1| HPr (Ser/Thr) protein kinase/phosphatase [Bacillus halodurans] sp|Q9K6Y4|HPRK_BACHD HPr(Ser) kinase/phosphatase pir||F84098 HPr (Ser/Thr) protein kinase/phosphatase BH3590 [imported] - Bacillus halodurans (strain C-125) dbj|BAB07309.1| HPr (Ser/Thr) protein kinase/phosphatase [Bacillus halodurans] Length = 310 Score = 391 bits (1005), Expect = e-108, Method: Compositional matrix adjust. Identities = 184/303 (60%), Positives = 243/303 (80%) Query: 4 KVKVSELVQNFQLEVVSGHEGLKRPITVDDLNRPGLEMAGYFEYYPEERVQLLGKTELAF 63 KV ++L++ FQLE++SG EG+ R IT D++RPG+EMAG+F YYP +R+QLLG+TEL+F Sbjct: 3 KVTANDLLERFQLELLSGEEGIHRSITTSDISRPGIEMAGFFTYYPAKRLQLLGRTELSF 62 Query: 64 FSMLPDEERKSRIRGICNDNTPCIVITRALDVPQELIDISNEKGLPVLRSSMATTIFSSR 123 + L +++ R+ +C +TP I+I+R L+VP EL+ S + G+PVLRS++ TT SS Sbjct: 63 YKQLSPVDKEERMSKLCTYDTPGIIISRGLEVPPELLKASEKVGVPVLRSNITTTRLSSM 122 Query: 124 LTSFLEGRLAPTATIHGVLCDVYGVGMLITGSSGIGKSETALELVKRGHRLIADDAVEIR 183 LT+FLE +LAPT +HGVL D+YG+G+LITGSSG+GKSETAL+LV+RGHRL+ADD+VEIR Sbjct: 123 LTNFLESQLAPTTAVHGVLVDIYGIGVLITGSSGVGKSETALDLVRRGHRLVADDSVEIR 182 Query: 184 QTSDNQLHGTAPELIRHLLEIRGVGIINVMTLFGAGAIRNHKRITLVVRLEAWQQDKQYD 243 + ++ L G +PELI+HLLEIRG+GIINVMTLFGAGA+R KRI L V LE W Q K YD Sbjct: 183 REHEDTLVGRSPELIQHLLEIRGLGIINVMTLFGAGAVRPFKRIALCVNLELWDQKKVYD 242 Query: 244 RLGLDEETTRIIDTDVPLVTIPVRPGRNLAVIIEVAAMNYRLKQMGFNAALQFTNKLTAT 303 RLGL E+ RI++ D+P +TIPVRPGRNLAVIIEVAAMN+RLK++G NAA QF+++L Sbjct: 243 RLGLSEDYLRIMNVDIPKLTIPVRPGRNLAVIIEVAAMNFRLKRLGINAAQQFSDRLNDV 302 Query: 304 ISE 306 I E Sbjct: 303 IEE 305 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.138 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 434,030,864 Number of extensions: 17178317 Number of successful extensions: 50710 Number of sequences better than 1.0e-05: 90 Number of HSP's gapped: 50642 Number of HSP's successfully gapped: 92 Length of query: 312 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 187 Effective length of database: 417,850,203 Effective search space: 78137987961 Effective search space used: 78137987961 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)