BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0148.p (327 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0225 [T] COG0642 Signal transduction histidine kinase 211 1e-54 lin1852 [T] COG0642 Signal transduction histidine kinase 205 7e-53 ML0132 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 150 4e-36 L0132 [T] COG0642 Signal transduction histidine kinase 150 4e-36 SP1632 [T] COG0642 Signal transduction histidine kinase 148 1e-35 >CAC0225 [T] COG0642 Signal transduction histidine kinase Length = 339 Score = 211 bits (537), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 106/250 (42%), Positives = 164/250 (65%), Gaps = 3/250 (1%) Query: 76 EPLSLEAEGYREAIEQLQIQHIRALNEAQMQQKEHYDFIISWFHEVKTPIAVLRLMQQT- 134 EPL + E Y IE L + + + + +++ +F+ +W HE+KTPIA +L+ ++ Sbjct: 85 EPLEYKDEVYISIIEDLYKDYNERIVSLEKEFEDNNEFMTAWIHEIKTPIATAKLLLESG 144 Query: 135 EIDHSSLEEELSRIEHYIDQALYYSKLDTFNQDYEIVNCNLELLVKEAVKAHSKTFISKK 194 EID EE+ +I+ Y+++ LYYS+ D F++DY I N+ L+KE +K+HSK FI K Sbjct: 145 EIDSQLFMEEIEKIDDYVEKVLYYSRSDNFSKDYIISEVNISKLIKECIKSHSKIFIKKH 204 Query: 195 IKIRLDVQATM-VQSDSKWLSFIINQLVTNSLKYTGDQGEISFTTQVTPHEKLLIVRDNG 253 IK++L++ V++D KW FI+NQ+++N+LKYT D G I +EK++I+ DNG Sbjct: 205 IKLKLEIDERFNVETDKKWFLFILNQIISNALKYTNDMGNIIIKAFEDDNEKVVIIEDNG 264 Query: 254 IGIDRRDLPRVFNRGFTGTNGRT-YAKSTGMGLYLAQELSKKLGHYISCESEAGSFSEFT 312 IGI + DL R+F++ FTG NGR +K+TGMGLYL+ +L++KLGH I+ ES+ ++ Sbjct: 265 IGIKKEDLDRIFSKSFTGYNGRKENSKATGMGLYLSNKLAEKLGHNITIESKYNEGTKLY 324 Query: 313 IHFPKNHDPY 322 IHFPK D Y Sbjct: 325 IHFPKWCDYY 334 >lin1852 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 205 bits (522), Expect = 7e-53, Method: Compositional matrix adjust. Identities = 0/256 (0%), Positives = 0/256 (0%), Gaps = 9/256 (3%) Query: 76 EPLSLEAEGYREAIEQLQIQHIRALNEAQMQQKEHYDFIISWFHEVKTPIAVLRLM---- 131 + + + + + E ++ + + Sbjct: 88 LGVEITPPESIYPDFRYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADV 147 Query: 132 --QQTEIDHSSLEEELSRIEHYIDQALYYSKLDTFNQDYEIVNCNLELLVKEAVKAHSKT 189 Sbjct: 148 LH 207 Query: 190 FISKKIKIRLDVQATMVQSDSKWLSFIINQLVTNSLKYTGDQGEISF--TTQVTPHEKLL 247 Sbjct: 208 - 266 Query: 248 IVRDNGIGIDRRDLPRVFNRGFTGTNGRTYAKSTGMGLYLAQELSKKLGHYISCESEAGS 307 Sbjct: 267 326 Query: 308 FSEFTIHFPKNHDPYL 323 Sbjct: 327 342 >ML0132 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Length = 211 Score = 150 bits (378), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 0/224 (0%), Positives = 0/224 (0%), Gaps = 10/224 (4%) Query: 90 EQLQIQHIRALNEAQMQQKEHYDFIISWFHEVKTPIAVLRLMQQTEIDHSSLEEELSRIE 149 L ++ + I+ H + P L + + L+ Sbjct: 75 YIKT----IGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRKTANVVLNTAF 130 Query: 150 HYIDQALYYSKLDTFNQDYEIVNCNLELLVKEAVKAHSKTFISKKIKIRLDVQATMVQSD 209 + A+ N+ N+E + ++ +K F + Sbjct: 131 GWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLILHGR-YTCIA 189 Query: 210 SKWLSFIINQLVTNSLKYTGDQGEISFTTQVTPHEKLLIVRDNGIGIDRRDLPRVFNRGF 269 Sbjct: 190 ----- 244 Query: 270 TGTNGRTYAKSTGMGLYLAQELSKKLGHYISCESEAGSFSEFTI 313 Sbjct: 245 288 >L0132 [T] COG0642 Signal transduction histidine kinase Length = 291 Score = 150 bits (378), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 83/224 (37%), Positives = 134/224 (59%), Gaps = 10/224 (4%) Query: 90 EQLQIQHIRALNEAQMQQKEHYDFIISWFHEVKTPIAVLRLMQQTEIDHSSLEEELSRIE 149 E+LQ + L + + ++E D I W H++K P+A + LM QT+++ L+ ++ +E Sbjct: 75 EELQ----KKLEQQNLAEREMEDIIRVWSHQMKVPLAAIDLMTQTQVNSEELKNQVFSLE 130 Query: 150 HYIDQALYYSKLDTFNQDYEIVNCNLELLVKEAVKAHSKTFISKKIKIRLDVQATMVQSD 209 +Y+ L Y +++ D+ ++ LVKE VK +S FI K + I+++ + +V SD Sbjct: 131 NYLKILLEYQRINNLATDFRFEKVSMASLVKELVKKYSSFFIQKNLSIQIEGEW-LVNSD 189 Query: 210 SKWLSFIINQLVTNSLKYTGDQGEISFTTQVTPHEKLLIVRDNGIGIDRRDLPRVFNRGF 269 +WLS + QL+ N++KYT + G ++ E LI++D+GIGI + DLPR+F GF Sbjct: 190 QRWLSLAVEQLLNNAVKYTKEGG---ILIKIKTGE--LIIQDSGIGILKEDLPRLFEHGF 244 Query: 270 TGTNGRTYAKSTGMGLYLAQELSKKLGHYISCESEAGSFSEFTI 313 TG NGR KSTG+GLYL++ + KL IS SE G+ + TI Sbjct: 245 TGYNGRIQQKSTGLGLYLSKLILDKLEFEISINSEIGNGTSVTI 288 >SP1632 [T] COG0642 Signal transduction histidine kinase Length = 324 Score = 148 bits (374), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 84/256 (32%), Positives = 145/256 (56%), Gaps = 11/256 (4%) Query: 65 QALRHLSDEDAEPLSLEAEGYREAIEQLQIQHIRALNEAQMQQKEHYDFIISWFHEVKTP 124 Q L + E PL + E +E +++ + ++A+ + + D+ W H++KTP Sbjct: 71 QELLYGEREAKSPLEI---ALAEKLEAREMELYQQRSKAERKLTDLLDYYTLWVHQIKTP 127 Query: 125 IAVLRLMQQTEIDHS---SLEEELSRIEHYIDQALYYSKLDTFNQDYEIVNCNLELLVKE 181 IA +L+ +D LE+E+ +I+ Y + L Y +L++F+ D + +E LVKE Sbjct: 128 IAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNLVLQYLRLESFHDDLVLKQVQIEDLVKE 187 Query: 182 AVKAHSKTFISKKIKIRLDVQATMVQSDSKWLSFIINQLVTNSLKYTGDQGEISFTTQVT 241 ++ ++ FI K + + L + +D KWL +I Q+++NSLKYT + G ++ Sbjct: 188 IIRKYALFFIQKGLNVNLHDLDKEIVTDKKWLLVVIEQIISNSLKYTKEGG-----LEIY 242 Query: 242 PHEKLLIVRDNGIGIDRRDLPRVFNRGFTGTNGRTYAKSTGMGLYLAQELSKKLGHYISC 301 ++ L ++D GIGI D+ RVF RGF+G NGR +S+G+GLYL++++S++LGH I Sbjct: 243 MDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGRLTQQSSGLGLYLSKKISEELGHQIRI 302 Query: 302 ESEAGSFSEFTIHFPK 317 ESE G + I F + Sbjct: 303 ESEVGKGTTVRIQFAQ 318 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,593,804 Number of extensions: 836474 Number of successful extensions: 2099 Number of sequences better than 1.0e-05: 146 Number of HSP's gapped: 2014 Number of HSP's successfully gapped: 146 Length of query: 327 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 220 Effective length of database: 26,037,693 Effective search space: 5728292460 Effective search space used: 5728292460 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)