BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0147.p (654 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2220 [V] COG0577 ABC-type antimicrobial peptide transport sys... 202 1e-51 lin1857 [V] COG0577 ABC-type antimicrobial peptide transport sys... 168 3e-41 CAC0227 [V] COG0577 ABC-type antimicrobial peptide transport sys... 149 1e-35 BS_ytsD [V] COG0577 ABC-type antimicrobial peptide transport sys... 136 1e-31 BH0752 [V] COG0577 ABC-type antimicrobial peptide transport syst... 129 2e-29 >lin2220 [V] COG0577 ABC-type antimicrobial peptide transport system, permease component Length = 289 Score = 202 bits (515), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 0/673 (0%), Positives = 0/673 (0%), Gaps = 54/673 (8%) Query: 1 MSLLELTVRNVKRNFRLYSIYLFSMIAGVIIQFTFTSLMYNKDIMDALQNRANFQTGVGI 60 M + + L ++ L + A + Sbjct: 1 MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLS 60 Query: 61 ASXXXXXXXXXXXXYANSFFMKQRKKEFGMYLLYGLNERQITRMVFYETLIMSAISLFTG 120 S + ++ F + E Sbjct: 61 KSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRSPEWSAFGIRPPLRL 120 Query: 121 ILIGGLLSKLFGMLLMDLMNYNQVISLSFPMPSIAATIGVFLLLAMIISVQSYIMLNRVQ 180 K + L+ Q S S S + + I Sbjct: 121 GEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVLYG 180 Query: 181 LTELFHAKQKMETPVRVSSAWAMIAVLLLGMAFALIS---SGKGSVFWQDYATVSMIAVT 237 L E ++ K + + + G+ +F + + Sbjct: 181 LNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGLGTGVQEFI 240 Query: 238 IGIIAGTFLFFRQLAGWLAQAVSRRKK-YYEGNTMLWTSSLRFQIRGNTLNLTFISLFSA 296 + + Sbjct: 241 LTVYGTCMPMLNLTRRKGQDIERYYPAE 300 Query: 297 AIMLLLSFMTINYKVQFEAVGRNLPNDIAFESLASSTNNRIDSLIRS----SGHEVRYHL 352 Sbjct: 301 -- 358 Query: 353 --TQEAIVAEPVSDMGPAFENPEYYTPYLLLVSEQTYNGLMQER-DPKQILDLQGEEAVT 409 Sbjct: 359 ---- 414 Query: 410 L--------AQGSDFAQSY--AANRQPEFKVKTDAETAFRIIEKKDYAYLGWSSDPVRSM 459 Sbjct: 415 ----------------- 457 Query: 460 VIKPAVLVISDE--AYRSLREHADKKSFEIYGIADAGRAEELSKQVHAIVTETPGAYYSS 517 Sbjct: 458 ----- 512 Query: 518 FADVYSKQIEXXXXXXXXXXXXXXXXXXXXXSVIYFKQLREATDERMQYTILRKMGVDDR 577 Sbjct: 513 572 Query: 578 QMKSVIRKQXXXXXXXXXXXGVCHSWFIIKYYILDSVQGFTGLTATVWGIMGVYFLIYVL 637 Sbjct: 573 --- 629 Query: 638 FYVSSTNVYYKIV 650 Sbjct: 630 642 >lin1857 [V] COG0577 ABC-type antimicrobial peptide transport system, permease component Length = 289 Score = 168 bits (425), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 0/667 (0%), Positives = 0/667 (0%), Gaps = 33/667 (4%) Query: 3 LLELTVRNVKRNFRLYSIYLFSMIAGVIIQFTFTSLMYNKDIMDALQNRANFQTGV-GIA 61 LE + + + +S + T I +++ + Sbjct: 13 WLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLSKSAVALRLTARP 72 Query: 62 SXXXXXXXXXXXXYANSFFMKQRKKEFGMYLLYGLNERQITRMVFYETLIMSAISLFTGI 121 + K++ + + Sbjct: 73 ILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRSPEWSAFGIRPPLRLGEFTMPEHKFVT 132 Query: 122 LIGGLLSKLFGMLLMDLMNYNQVISLSFPMPSIAATIGVFLLLAMIISVQSYIMLNRVQL 181 L L + L + + ++ + Sbjct: 133 LEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVLYGLNETRPSQDKDD 192 Query: 182 TELFHAKQKMETPVRVSSAWAMIAVLLLGMAFALISSG-KGSVFWQDYATVSMIAVTI-G 239 + + V+L G + + + G+ + TV + + Sbjct: 193 EQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLN 252 Query: 240 IIAGTFLFFRQLAGWLAQAV-SRRKKYYEGNTMLWTSSLRFQIRGNTLNLTFISLFSAAI 298 + + Sbjct: 253 LTRRKGQDIERYYPAEDAKAG 312 Query: 299 MLLLSFMTINYKVQFEAVGRNLPNDIAFESLASSTNNRIDSLIRSSGHEVRYHLTQEAIV 358 Sbjct: 313 ---- 368 Query: 359 AEPVSDMGPAFENPEYYTPY----LLLVSEQTYN--GLMQERDPKQILDLQGEEAVTLAQ 412 Sbjct: 369 428 Query: 413 GSDFAQSYAANRQPEFK--VKTDAETAFRIIEKKDYAYLGWSSDPVRSMVIKPAVLVISD 470 Sbjct: 429 --- 485 Query: 471 EAYRSLREHADKKSFEIYGIADAGRAEELSKQVHAIVTETPGAY---YSSFADVYSKQIE 527 Sbjct: 486 545 Query: 528 XXXXXXXXXXXXXXXXXXXXXSVIYFKQLREATDERMQYTILRKMGVDDRQMKSVIRKQX 587 Sbjct: 546 605 Query: 588 XXXXXXXXXXGVCHS----WFIIKYYILDSVQGFTGLTATVWGIMGVYFLIYVLFYVSST 643 Sbjct: 606 ------- 658 Query: 644 NVYYKIV 650 Sbjct: 659 665 >CAC0227 [V] COG0577 ABC-type antimicrobial peptide transport system, permease component Length = 289 Score = 149 bits (377), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 0/602 (0%), Positives = 0/602 (0%), Gaps = 47/602 (7%) Query: 1 MSLLELTVRNVKRNFRLYSIYLFSMIAGVIIQFTFTSLMYNKDIMDALQNRANFQTGVGI 60 M + + L ++ L + A + Sbjct: 1 MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLS 60 Query: 61 ASXXXXXXXXXXXXYANSFFMKQRKKEFGMYLLYGLNERQITRMVFYETLIMSAISLFTG 120 S + ++ F + E Sbjct: 61 KSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRSPEWSAFGIRPPLRL 120 Query: 121 ILIGGLLSKLFGMLLMDLMNYNQVISLSFPMPSIAATIGVFLLLAMIISVQSYIMLNRVQ 180 K + L+ Q S S S + + I Sbjct: 121 GEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVLYG 180 Query: 181 LTELFHAKQKMETPVRVSS---AWAMIAVLLLGMAFALISSGKGSVFWQDYATVSMIAVT 237 L E ++ K + + A +L ++ G+ +F + + Sbjct: 181 LNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGLGTGVQEF- 239 Query: 238 IGIIAGTFLFFRQLAGWLAQAVSRRKKY-YEGNTMLWTSSLRFQIRGNTLNLTFISLFSA 296 ++ + + Sbjct: 240 -----ILTVYGTCMPMLNLTRRKGQDIER 294 Query: 297 AIMLLLSFMTINYKVQFEAVGRNL-PNDIAFESLASSTNNRIDSLIRSSGH-EVRYHLTQ 354 Sbjct: 295 - 353 Query: 355 EAIVAEPVSDMGPAFENPEYYTPYLLLVSEQTYNGLMQERDPKQILDLQGEEAVTLAQGS 414 Sbjct: 354 ------------- 400 Query: 415 DFAQSYAANRQPEFKVKTDAETAF-------RIIEKKDYAYLGWSSDPVRSMVIKPAVLV 467 Sbjct: 401 --------- 451 Query: 468 ISDEAYRSLREHADKKSF---EIYGIADAGRAEELSKQVHAIVTETPGAYYSSFADVYSK 524 Sbjct: 452 -- 509 Query: 525 QIEXXXXXXXXXXXXXXXXXXXXXSVIYFKQLREATDERMQYTILRKMGVDDRQMKSVIR 584 Sbjct: 510 569 Query: 585 KQ 586 Sbjct: 570 571 >BS_ytsD [V] COG0577 ABC-type antimicrobial peptide transport system, permease component Length = 289 Score = 136 bits (343), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 0/692 (0%), Positives = 0/692 (0%), Gaps = 86/692 (12%) Query: 1 MSLLELTVRNVKRNFRLYSIYLFSMIAGVIIQFTFTSLMYNKDIMDALQNRANFQTGVGI 60 M + + L ++ L + A Sbjct: 1 MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTG---HAIGAYIRAR 57 Query: 61 --ASXXXXXXXXXXXXYANSFFMKQRKKEFGMYLLYGLNERQITRMVFYETLIMSAISLF 118 + + + ++ + I Sbjct: 58 RLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRSPEWSAFGIRPP 117 Query: 119 TGILIGGLLSKLFGMLLMDLMNYNQVISLSFPMPSIAATIGVFLLLAMIISVQSYIMLNR 178 + + F L + + + + Sbjct: 118 LRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPV 177 Query: 179 VQLTELFHAKQKMETPVRVSSAWAMIAVLLLGMAFALISSG---KGSVFWQDYATVSMIA 235 + Q + V A+ +++ + + + Sbjct: 178 LYGLNETRPSQDKDDEQEVFYTTAL---AQDQADGYVLTGHPVMLQGGEYVMFTYEGLGT 234 Query: 236 VTI-----GIIAGTFLFFRQLAGWLAQAVSRRK-KYYEGNTMLWTSSLRFQIRGNTLNLT 289 + Sbjct: 235 GVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAE 294 Query: 290 FISLFSAAIMLLLSFMTINYKVQFEAVGRNLPNDIAFESLASSTNNRIDSLIRSSGHEVR 349 Sbjct: 295 ------ 348 Query: 350 YHLTQEAIVAEPVSDMGPAFENPEYYTPYLLLVSEQTYNGLMQ-----ERDPKQILDLQG 404 Sbjct: 349 -------- 400 Query: 405 EEAVTLAQGSDFAQSYAANRQPE-FKVKTDAETAFRIIEKKDYAYLGWSSDPVRSMVIK- 462 Sbjct: 401 -------- 452 Query: 463 ---PAVLVISDEAYRSLREHADKK---------SFEIYGIADAGRAEELSKQV-----HA 505 Sbjct: 453 - 511 Query: 506 IVTETPGAYYSSFADVYSKQIEXXXXXXXXXXXXXXXXXXXXXSVIYFKQLREATDERMQ 565 Sbjct: 512 ----------- 560 Query: 566 YTILRKMGVDDRQMKSVIRKQXXXXXXXXXXXGVCHSWFIIKYYILDSVQGFTGLTATVW 625 Sbjct: 561 ------- 613 Query: 626 G----IMGVYFLIYVLFYVSSTNVYYKIVNEN 653 Sbjct: 614 645 >BH0752 [V] COG0577 ABC-type antimicrobial peptide transport system, permease component Length = 289 Score = 129 bits (324), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 0/676 (0%), Positives = 0/676 (0%), Gaps = 63/676 (9%) Query: 1 MSLLELTVRNVKRNFRLYSIYLFSMIAGVIIQFTFTSLMYNKDIMDALQNRANFQTGVGI 60 M + + L ++ L + + + Sbjct: 1 MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTG-HAIGAYIRARRL 59 Query: 61 ASXXXXXXXXXXXX-YANSFFMKQRKKEFGMYLLYGLNERQITRMVFYETLIMSAISLFT 119 + + ++ F + E Sbjct: 60 SKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRSPEWSAFGIRPPLR 119 Query: 120 GILIGGLLSKLFGMLLMDLMNYNQVISLSFPMPSIAATIGVFLLLAMIISVQSYIMLNRV 179 K + L+ Q S S S + + I Sbjct: 120 LGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVLY 179 Query: 180 QLTELFHAKQKMETPVRVSSAWAMIAVLLLGMAFALISSGKGSVFWQDYATVSMIAVTIG 239 L E ++ K + + + +G + + Sbjct: 180 GLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYV-----MFTYEGLGT 234 Query: 240 IIAGTFLFFRQLA-GWLAQAVSRRKKYYEGNTMLWTSSLRFQIRGNTLNLTFISLFSAAI 298 + L Sbjct: 235 GVQEFILTVYGTCM 294 Query: 299 M----LLLSF-----MTINYKVQFEAVGRNLPNDIAFESLASSTNNRIDSLIRSSGHEVR 349 Sbjct: 295 -------- 346 Query: 350 YHLTQEAIVAEPVSDMGPAFENPEYYTPYLLLVSEQTYNGLMQERDPKQILDLQGEEAVT 409 Sbjct: 347 ------- 399 Query: 410 LAQGSDFAQSYA-ANRQPEFKVKTDAETAFRIIEKKDYAYLGWSSDPVRSMVIKPA---- 464 Sbjct: 400 ------ 453 Query: 465 VLVISDEAYRSLR-EHADKKSFEI--YGIADAGRAEELSKQVHAIVTETPGAYYSSFA-- 519 Sbjct: 454 513 Query: 520 -DVYSKQIEXXXXXXXXXXXXXXXXXXXXXSVIYFKQLREATDERMQYTILRKMGVDDRQ 578 Sbjct: 514 ---- 569 Query: 579 MKSVIRKQXXXXXXXXXXXGVCHSWFIIKYYILDSVQGFTGLTATVWGI----MGVYFLI 634 Sbjct: 570 ------ 623 Query: 635 YVLFYVSSTNVYYKIV 650 Sbjct: 624 639 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.136 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 39,486,335 Number of extensions: 1438183 Number of successful extensions: 14089 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 14058 Number of HSP's successfully gapped: 18 Length of query: 654 Length of database: 38,378,431 Length adjustment: 113 Effective length of query: 541 Effective length of database: 25,345,689 Effective search space: 13712017749 Effective search space used: 13712017749 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 119 (50.4 bits)