BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0140.p (248 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VNG0390G [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphory... 124 2e-28 PH0380 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphoryla... 99 5e-21 PAB1053 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphoryl... 87 2e-17 CAC2237 [G] COG0448 ADP-glucose pyrophosphorylase 54 2e-07 FN0855 [G] COG0448 ADP-glucose pyrophosphorylase 54 2e-07 >VNG0390G [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) Length = 342 Score = 124 bits (310), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 80/243 (32%), Positives = 114/243 (46%), Gaps = 5/243 (2%) Query: 1 MIALILAAGYATRXXXXXXXXXXXXXXVQGSRTIXXXXXXXXXXXXXXXXXXIVTNDRFF 60 M A++LA GYATR V G T+ + TN+RF Sbjct: 14 MKAVVLAGGYATRLWPITKDRPKMFLPV-GDSTVIDRIFADLESDDRIDTVYVSTNERFA 72 Query: 61 RAFEQWSQKYRGTKPIRILNDGTTSPEGRLGAIGDIQFAIGREQIQDDLLVLAGDNVLGF 120 F + KP + D TT + G +G + + RE I DDL+V+AGDN++ F Sbjct: 73 DEFRSHLAERSFEKPQLSVED-TTDENEKFGVMGALAQLVDRESITDDLVVIAGDNLISF 131 Query: 121 GLEEYLSYFHQVDKD--CILVRTVDDIRELRSVGVAELDAEMRVLSLEEKPQAPRTNIGV 178 + ++ +F D D I V +S G+ EL + V++ +EKP P + + Sbjct: 132 DVSAFVDFFAADDADNPAIAAYDVGSYERAQSYGLVELHGD-EVINFQEKPDDPNSTLVS 190 Query: 179 FALYIYKRSTLPLFSRYLAEGGNPDAPSYFPEWLHSRQEIRAYYTEGAIDDVGTPEAYAE 238 A Y Y S + YLA GNPD P +F EWL RQ +RA+ EGA D+GTPE+Y + Sbjct: 191 IACYAYPESVVEDIDTYLAADGNPDEPGWFVEWLQDRQSVRAFTFEGAWFDIGTPESYLD 250 Query: 239 MRA 241 A Sbjct: 251 AVA 253 >PH0380 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) Length = 327 Score = 99.0 bits (245), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 76/246 (30%), Positives = 121/246 (49%), Gaps = 17/246 (6%) Query: 1 MIALILAAGYATRXXXXXXXXXXXXXXVQGSRTIXXXXXXXXXXXXXXXXXXIVTNDRFF 60 M LI+A GYATR + G++TI ++ +RFF Sbjct: 1 MKVLIMAGGYATRLWPITKDNPKALLPL-GNKTILEYILEKVRELDVDVY---ISTNRFF 56 Query: 61 RAFEQWSQKYRGTKPIRILNDGTTSPEGRLGAIGDIQFAIGREQIQDDLLVLAGDNVLGF 120 ++K+R + ++ + T E +LG I ++ A+ +DD L++AGDNV F Sbjct: 57 ------AEKFRKYD-VELIVEDTVHEEEKLGTIAAMRNALNILG-EDDYLIVAGDNVFSF 108 Query: 121 GLEEYLSYFHQVDKDCILVRTVDDIRELRSVGVAELDAEMRVLSLEEKPQAPRTNIGVFA 180 L++++ F K I V V D + GV L+ + RV+ EEKP P++ + Sbjct: 109 SLKDFVDNFSG--KTLIAVYDVGDFELAKRYGVVVLEKD-RVIDFEEKPANPKSTLISTG 165 Query: 181 LYIYKRSTLPLFSRYLAEGGNPDAPSYFPEWLHSR-QEIRAYYTEGAIDDVGTPEAYAEM 239 +Y++ R + L + YL E GN D+P YF +WL R +EIRAY +G D+G+ ++Y E Sbjct: 166 VYLFPRDVMKLLNEYL-EDGNKDSPGYFLQWLLKRGEEIRAYRFDGYWYDIGSADSYLEA 224 Query: 240 RARLGK 245 L K Sbjct: 225 MKTLLK 230 >PAB1053 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) Length = 332 Score = 87.4 bits (215), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 76/246 (30%), Positives = 113/246 (45%), Gaps = 12/246 (4%) Query: 1 MIALILAAGYATRXXXXXXXXXXXXXXVQGSRTIXXXXXXXXXXXXXXXXXXIVTNDRFF 60 M LI+A GYATR V G +TI I TN F Sbjct: 1 MKVLIMAGGYATRLWPLTKDKPKPLLPV-GIKTI-LDFIMEKVLELNVDEVYISTNKFFE 58 Query: 61 RAFEQWSQKYRGTKPIRILNDGTTSPEGRLGAIGDIQFAIGREQIQDDLLVLAGDNVLGF 120 F+ +++KY + ++ + T E +LG IG I+ A+ + DD L++AGDN+ Sbjct: 59 HKFKPYAEKYG----VDLIIEDTYREEEKLGTIGAIKNALDSLGL-DDYLIIAGDNIFSL 113 Query: 121 GLEEYLSYFHQVDKDCILVRTVDDIRELRSVGVAELDAEMRVLSLEEKPQAPRTNIGVFA 180 L +++S F K I V V D+ + GV L+ RV+ EKPQ P + + Sbjct: 114 SLRDFVSKF--TGKPLIAVYDVGDLELAKRYGVVILEGN-RVVKFIEKPQEPPSTLISTG 170 Query: 181 LYIYKRSTLPLFSRYLAEGGNPDAPSYFPEWLHSRQ-EIRAYYTEGAIDDVGTPEAYAEM 239 +Y R + + YL + GN DAP YF +WL R E+ AY + D+G+ ++Y E Sbjct: 171 IYALPRDVMGMIDEYL-KYGNKDAPGYFIQWLIERGIEVYAYKFDDFWYDIGSADSYLES 229 Query: 240 RARLGK 245 L K Sbjct: 230 LKMLMK 235 >CAC2237 [G] COG0448 ADP-glucose pyrophosphorylase Length = 380 Score = 54.3 bits (129), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 35/138 (25%), Positives = 62/138 (44%), Gaps = 3/138 (2%) Query: 109 LLVLAGDNVLGFGLEEYLSYFHQVDKDCILVRTVDDIRELRSVGVAELDAEMRVLSLEEK 168 +++L+GD++ E L + + + D + + E G+ D + R+ EEK Sbjct: 122 VVILSGDHIYKMNYLEMLDFHKKKNADATIAVFEVPLNEASRFGIMNTDEDDRINEFEEK 181 Query: 169 PQAPRTNIGVFALYIYKRSTLPLFSRYLA--EGGNPD-APSYFPEWLHSRQEIRAYYTEG 225 P+ P+ N+ +YI+ L F A E N D + P L+ ++++ AY G Sbjct: 182 PKKPKNNLASMGIYIFNWRFLKRFLEEDARDESSNNDFGKNIIPNMLNKKRKLYAYRFNG 241 Query: 226 AIDDVGTPEAYAEMRARL 243 DVGT ++ E L Sbjct: 242 YWKDVGTIQSLWEANMDL 259 >FN0855 [G] COG0448 ADP-glucose pyrophosphorylase Length = 384 Score = 53.9 bits (128), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 34/141 (24%), Positives = 69/141 (48%), Gaps = 9/141 (6%) Query: 109 LLVLAGDNVLGFGLEEYLSYFHQVDKDCILVRTVDDIRELRSVGVAELDAEMRVLSLEEK 168 +L+L+GD++ ++ L + Q D D + + + S G+ +M + EEK Sbjct: 125 VLILSGDHIYKMNYDKMLQFHIQKDADATIGVFKVPLVDAPSFGIMNTKDDMSIYEFEEK 184 Query: 169 PQAPRTNIGVFALYIYKRSTLPLFSRYLAEG-GNPDAPSYF-----PEWLHSRQEIRAYY 222 P+ P++++ +YI+ L +YL E +P++ + F P L+ +++ AY Sbjct: 185 PKEPKSDLASMGIYIF---NWKLLKKYLDEDEKDPNSSNDFGKNIIPNMLNDGKKMFAYP 241 Query: 223 TEGAIDDVGTPEAYAEMRARL 243 +G DVGT +++ + L Sbjct: 242 FKGYWRDVGTIQSFWDAHMDL 262 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.139 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,845,934 Number of extensions: 721175 Number of successful extensions: 2049 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 2050 Number of HSP's successfully gapped: 12 Length of query: 248 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 144 Effective length of database: 26,383,695 Effective search space: 3799252080 Effective search space used: 3799252080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)