BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0138.p (308 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all2891 [R] COG0300 Short-chain dehydrogenases of various substr... 202 7e-52 Rv1544 [R] COG0300 Short-chain dehydrogenases of various substra... 189 5e-48 MT1596 [R] COG0300 Short-chain dehydrogenases of various substra... 189 5e-48 all3753 [R] COG0300 Short-chain dehydrogenases of various substr... 186 4e-47 AGl1280 [R] COG0300 Short-chain dehydrogenases of various substr... 174 1e-43 >all2891 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Length = 258 Score = 202 bits (513), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 105/248 (42%), Positives = 154/248 (62%), Gaps = 4/248 (1%) Query: 55 SLITGASSGIGEQFARQLAKEGSHLVLVARSKRKLDTLAAELTRSYGIQCQVVPQDLSIE 114 +LITGAS GIG+ FA++LA ++LVLVARS+ KL LA EL + + IQ V+ +DL+ Sbjct: 4 ALITGASGGIGKAFAQELAARQTNLVLVARSQHKLHQLAQELQQQHKIQVDVIAKDLTEI 63 Query: 115 GAAGELYQKCQQLNLNIDLLVNNAGFATHGLFEQISGERQHEEVMLNVAAVVDMTHLFLP 174 A +++ + L ID L+NNAGF +G F Q RQ + + LNV A+VD+TH FLP Sbjct: 64 DAVADVFDITKSKGLTIDCLINNAGFGDYGDFAQSDRTRQIKIIQLNVLALVDLTHRFLP 123 Query: 175 GMLHRGAGAVINVSSTAGFQPLPYMAVYGATKAFVLSFTDALWWENRDRGVQFFALCPGS 234 M +G++INV+S AGFQP+PY++VY A+KAF++SF++ALW ENR GV+ CPG Sbjct: 124 LMRQNRSGSIINVASIAGFQPIPYLSVYAASKAFIVSFSEALWAENRQYGVRVLVTCPGP 183 Query: 235 TETNFFNVVGTEDASVGAKDTPERVVEVTLRAMKKGKQY----VVPGLRNYLGAQISRFM 290 TET+FF A V + EV L+++K + + ++ L + ++R + Sbjct: 184 TETDFFAEANFPQALVETTNKVMSSEEVVLKSLKALENWEPTVIISDTSTQLRSNVARLV 243 Query: 291 TRRQSLRL 298 R+ L L Sbjct: 244 PRKTLLSL 251 >Rv1544 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Length = 267 Score = 189 bits (480), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 105/238 (44%), Positives = 148/238 (62%), Gaps = 3/238 (1%) Query: 56 LITGASSGIGEQFARQLAKEGSHLVLVARSKRKLDTLAAELTRSYGIQCQVVPQDLSIEG 115 +ITGASSGIG + AR LA G L+LVAR + +LD LA +L + + + +V+P DL+ Sbjct: 13 VITGASSGIGVELARGLAGRGFPLMLVARRRERLDELADQLRQEHCVGVEVLPLDLADTQ 72 Query: 116 AAGELYQKCQQLNLNIDLLVNNAGFATHGLFEQISGERQHEEVMLNVAAVVDMTHLFLPG 175 A +L + + + I L N+AGF T G F ++ R+ EEV+LN A++++TH LPG Sbjct: 73 ARAQLADRLR--SDAIAGLCNSAGFGTSGRFWELPFARESEEVVLNALALMELTHAALPG 130 Query: 176 MLHRGAGAVINVSSTAGFQPLPYMAVYGATKAFVLSFTDALWWENRDRGVQFFALCPGST 235 M+ RGAGAV+N++S AGFQP+PYMAVY ATKAFVL+F++A+ E GV ALCPG Sbjct: 131 MVKRGAGAVLNIASIAGFQPIPYMAVYSATKAFVLTFSEAVQEELHGTGVSVTALCPGPV 190 Query: 236 ETNFFNVVGTEDASVG-AKDTPERVVEVTLRAMKKGKQYVVPGLRNYLGAQISRFMTR 292 T + + E S+ A+ +P V E + M GK+ VVPG+ + RF R Sbjct: 191 PTEWAEIASAERFSIPLAQVSPHDVAEAAIAGMLSGKRTVVPGIVPKFVSTSGRFAPR 248 >MT1596 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Length = 267 Score = 189 bits (480), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 105/238 (44%), Positives = 148/238 (62%), Gaps = 3/238 (1%) Query: 56 LITGASSGIGEQFARQLAKEGSHLVLVARSKRKLDTLAAELTRSYGIQCQVVPQDLSIEG 115 +ITGASSGIG + AR LA G L+LVAR + +LD LA +L + + + +V+P DL+ Sbjct: 13 VITGASSGIGVELARGLAGRGFPLMLVARRRERLDELADQLRQEHCVGVEVLPLDLADTQ 72 Query: 116 AAGELYQKCQQLNLNIDLLVNNAGFATHGLFEQISGERQHEEVMLNVAAVVDMTHLFLPG 175 A +L + + + I L N+AGF T G F ++ R+ EEV+LN A++++TH LPG Sbjct: 73 ARAQLADRLR--SDAIAGLCNSAGFGTSGRFWELPFARESEEVVLNALALMELTHAALPG 130 Query: 176 MLHRGAGAVINVSSTAGFQPLPYMAVYGATKAFVLSFTDALWWENRDRGVQFFALCPGST 235 M+ RGAGAV+N++S AGFQP+PYMAVY ATKAFVL+F++A+ E GV ALCPG Sbjct: 131 MVKRGAGAVLNIASIAGFQPIPYMAVYSATKAFVLTFSEAVQEELHGTGVSVTALCPGPV 190 Query: 236 ETNFFNVVGTEDASVG-AKDTPERVVEVTLRAMKKGKQYVVPGLRNYLGAQISRFMTR 292 T + + E S+ A+ +P V E + M GK+ VVPG+ + RF R Sbjct: 191 PTEWAEIASAERFSIPLAQVSPHDVAEAAIAGMLSGKRTVVPGIVPKFVSTSGRFAPR 248 >all3753 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Length = 263 Score = 186 bits (472), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 101/253 (39%), Positives = 152/253 (60%), Gaps = 3/253 (1%) Query: 53 KWSLITGASSGIGEQFARQLAKEGSHLVLVARSKRKLDTLAAELTRSYGIQCQVVPQDLS 112 + +LITGA+SGIG + A+ +LVLV R+K KL+ +A E +GI + + +DLS Sbjct: 7 QTALITGAASGIGYELVCIFAENDYNLVLVDRAKEKLEEIAIEFQDKFGISVKPIVRDLS 66 Query: 113 IEGAAGELYQKCQQLNLNIDLLVNNAGFATHGLFEQISGERQHEEVMLNVAAVVDMTHLF 172 + E++Q+ +Q N+ +D+LVNNAGF T+GLF + + E + +NV +T LF Sbjct: 67 KTTSPEEIFQEVEQANIKVDVLVNNAGFGTYGLFNDTNLTDELEMLQVNVVCTTHLTKLF 126 Query: 173 LPGMLHRGAGAVINVSSTAGFQPLPYMAVYGATKAFVLSFTDALWWENRDRGVQFFALCP 232 L M+ +G G ++NVSS A FQP P MAVY ATKA+VLSF++AL E GV LCP Sbjct: 127 LKNMVQQGEGKILNVSSAAAFQPGPLMAVYFATKAYVLSFSEALANELDGTGVTVTVLCP 186 Query: 233 GSTETNFFNVVGTEDAS-VGAKDTPE--RVVEVTLRAMKKGKQYVVPGLRNYLGAQISRF 289 G+T++ F G D+ V K + V ++ R + +GK V+PGL N + A+ RF Sbjct: 187 GTTQSAFHQRTGMADSKLVKGKRMMDASTVADIGYRGLMQGKTIVIPGLINKIMAKSIRF 246 Query: 290 MTRRQSLRLAQGM 302 + R+ ++ + M Sbjct: 247 IPRKLVTKIVRNM 259 >AGl1280 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Length = 134 Score = 174 bits (442), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 0/258 (0%), Positives = 0/258 (0%), Gaps = 19/258 (7%) Query: 52 GKWSLITGASSGIGEQFARQLAKEGSHLVLVARSKRKLDTLAAELTRSYGIQCQVVPQDL 111 Q + + + + + + ++ + Sbjct: 36 AVSYKSFQELPKAKAPAVLQKLAQAVAAEGFSGIQINKALSSIDAHQETSGSGRIQTLRV 95 Query: 112 SIEGAAGELYQKCQQLNLNIDLLVNNAGFATHGLFEQISGERQHEEVMLNVAAVVDMTHL 171 Sbjct: 96 155 Query: 172 FLPGMLHRGAGAVINVSSTAGFQPLPYMAVYGATKAFVLSFTDALWWENRDRGVQFFALC 231 Sbjct: 156 215 Query: 232 PGSTETNFFNVVGTEDASVGAKDT---------PERVVEVTLRAMKKGKQYVVPGLRNYL 282 Sbjct: 216 ------- 268 Query: 283 GAQISRFMTR---RQSLR 297 Sbjct: 269 286 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,010,598 Number of extensions: 895130 Number of successful extensions: 2567 Number of sequences better than 1.0e-05: 295 Number of HSP's gapped: 2292 Number of HSP's successfully gapped: 295 Length of query: 308 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 202 Effective length of database: 26,153,027 Effective search space: 5282911454 Effective search space used: 5282911454 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)