BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0123.p (409 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2459 [G] COG1455 Phosphotransferase system cellobiose-specifi... 116 5e-26 BS_licC [G] COG1455 Phosphotransferase system cellobiose-specifi... 114 3e-25 lin0874 [G] COG1455 Phosphotransferase system cellobiose-specifi... 114 3e-25 SP0310 [G] COG1455 Phosphotransferase system cellobiose-specific... 112 8e-25 VC1282 [G] COG1455 Phosphotransferase system cellobiose-specific... 110 3e-24 >lin2459 [G] COG1455 Phosphotransferase system cellobiose-specific component IIC Length = 301 Score = 116 bits (291), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 0/431 (0%), Positives = 0/431 (0%), Gaps = 39/431 (9%) Query: 1 MNKLIEWLKNDFSPKMNKVNNNVWVITLKDSVMQLLPFILLGSVFCLLT-------IPGD 53 M+ + V + + L + + + ++ Sbjct: 1 MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIY 60 Query: 54 VYHIKNY-PDFWVPFGW-TMGMLSLLVSFLIPFNLMEKKKLRKQRFIAGCTSLITFLIIV 111 V + + S + + ++E + + + + Sbjct: 61 VDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEM--VLNKLREGKAP 118 Query: 112 TPQVVKDGTVGFKHSSLGTGGMFVAIIAALITGYIMSLFG---KFSFFKEESVIPDFVRA 168 V + +A+ + + + +++ + Sbjct: 119 VILAVNKVD---NVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPE 175 Query: 169 WFDSMLPIGVTI----ILAWVLVDLVHIDIYNIILNIFMPLASVVETPWGFMLILFISCF 224 P + + + + + I+ Sbjct: 176 -ATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGL 234 Query: 225 LYSMGISSWILTPVTKPIFLAAIMANATAGAHNLVTAETIYS------AYLW----VGGV 274 + + A I ++ Sbjct: 235 ILVEREGQK---KMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLELWVKVKSGWADDERA 291 Query: 275 ACTLPLAFMHLLSKSTKLKALGRASLVPSIFNINEPLVFG-SIVWNPIMMLPMWINGLVL 333 Sbjct: 292 351 Query: 334 PLIVWIGTKVIHFGPIPKVVFDMWYVPFPXXXXXXXXXXXXXXXXXXXXXAATLIWYPFF 393 Sbjct: 352 --- 408 Query: 394 KAYEKQELEKE 404 Sbjct: 409 419 >BS_licC [G] COG1455 Phosphotransferase system cellobiose-specific component IIC Length = 452 Score = 114 bits (285), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 117/436 (26%), Positives = 202/436 (46%), Gaps = 35/436 (8%) Query: 1 MNKLIEWLKNDFSPKMNKVNNNVWVITLKDSVMQLLPFILLGSVFCL---LTIPGDV-YH 56 MNK+ + L+ P ++ + L+D ++ +P I++GS F + L IPG + Sbjct: 1 MNKVNQILEEKVMPIAGRIAGQRHLQALRDGIILTMPLIIIGSFFLIIGNLPIPGYAEFM 60 Query: 57 IKNYPDFWV-----PFGWTMGMLSLLVSFLIPFNLMEKKKLRKQRFIAGCTSLITFLIIV 111 K + W P T ++ L+ +F I + L EK + AG SL FL+ Sbjct: 61 AKTFGSSWSEKLAYPVDATFEIMGLVAAFGIAYRLAEKYGV--DALSAGAISLAAFLLAT 118 Query: 112 TPQV--VKDGTV-------GFKHSSLGTGGMFVAIIAALITGYIMSLFGKFSF-FKEESV 161 QV + DG G S +G+ G+FVA+I A+++ I L + + FK Sbjct: 119 PYQVPFMPDGATKEIMVGGGIPLSLMGSKGLFVAMIIAMVSTEIYRLIIQRNLVFKMPDG 178 Query: 162 IPDFVRAWFDSMLPIGVTIILAWVLVDLVHIDIYNIILNIF-----MPLASVVETPWGFM 216 +P V F +++P I L W +V + + NI PL+ + + G + Sbjct: 179 VPPAVSKSFVALIPGFAVIFLIWAARLIVEATPFESLHNIVSVLLGTPLSILGGSLGGSL 238 Query: 217 LILFISCFLYSMGI-SSWILTPVTKPIFLAAIMANATA--GAHNL--VTAETIYSAYLWV 271 + + L++ G+ + I+ V PI+ A+ AN A L + + + ++ + Sbjct: 239 VAEAVKMLLWACGLHGANIVGGVMAPIWYGAMDANRIAFQAGEELPKIFTQQFFDIWVNI 298 Query: 272 GGVACTLPLAF-MHLLSKSTKLKALGRASLVPSIFNINEPLVFG-SIVWNPIMMLPMWIN 329 GG TL L M L ++S ++K LG+ ++ P+IFNINEP++FG IV NP+++LP I Sbjct: 299 GGSGATLALVVTMFLRARSKQMKQLGKLAVGPAIFNINEPIIFGMPIVMNPMLLLPFIIT 358 Query: 330 GLVLPLIVWIGTKVIHFGPIPKVVFDMWYVPFPXXXXXXXXXXXXXXXXXXXXXAAT-LI 388 LV + +IG P + W +P A + ++ Sbjct: 359 PLVTVTLTYIGMST-GLVAKPAGIAVPWTMPPIFSGYLATGGKVSGAVMQAINIAVSFVV 417 Query: 389 WYPFFKAYEKQELEKE 404 +YPFF+ ++KQ+L++E Sbjct: 418 YYPFFRMWDKQKLKEE 433 >lin0874 [G] COG1455 Phosphotransferase system cellobiose-specific component IIC Length = 301 Score = 114 bits (285), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 0/427 (0%), Positives = 0/427 (0%), Gaps = 35/427 (8%) Query: 1 MNKLIEWLKNDFSPKMNKVNNNVWVITLKDSVMQLLPFILLGSVFCLLTIPGDVY----- 55 ++ S +NK+ IT + + + + + I D Sbjct: 10 FIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTPGLHME 69 Query: 56 ----HIKNYPDFWVPFGWTMGMLSLLVSFLIPFNLMEKKKLRKQRFIAGCTSLITFLIIV 111 + + ++ +V + + + + ++ + Sbjct: 70 EKRAINRLMNKAASSSIGDVELVIFVVEGTRWTP--DDEMVLNKLREGKAPVILAVNKVD 127 Query: 112 TPQVVKDGTVGFKHSSLGTGGMFVAIIAALITGYIMSLFGKFSFFKEESVIPDFVRAWFD 171 Q + + I+ + + + D Sbjct: 128 NVQE--KADLLPHL--QFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPED 183 Query: 172 SMLP--------IGVTIILAWVLVDLVHIDIYNIILNIFMPLASVVETPWGFMLILFISC 223 + + L L + + I +++ LIL Sbjct: 184 YITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIER---FVSNERGGYDINGLILVERE 240 Query: 224 FLYSMGISSW-ILTPVTKPI---FLAAIMANATAGAH-NLVTAETIYSAYLWVGGVACTL 278 M I + + + V + Sbjct: 241 GQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLELWVKVKSGWADDERALRSLGYVDD 300 Query: 279 PLAFMHLLSKSTKLKALGRASLVPSIFNINEPLVFG-SIVWNPIMMLPMWINGLVLPLIV 337 Sbjct: 301 360 Query: 338 WIGTKVIHFGPIPKVVFDMWYVPFPXXXXXXXXXXXXXXXXXXXXXAATLIWYPFFKAYE 397 Sbjct: 361 --- 417 Query: 398 KQELEKE 404 Sbjct: 418 424 >SP0310 [G] COG1455 Phosphotransferase system cellobiose-specific component IIC Length = 448 Score = 112 bits (281), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 119/454 (26%), Positives = 203/454 (44%), Gaps = 64/454 (14%) Query: 2 NKLIEWLKNDFSPKMNKVNNNVWVITLKDSVMQLLPFILLGSVFCLLTI----------P 51 N L +L+N M K+ V + + M +PF ++GS+F + +I Sbjct: 5 NGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIVGSMFLVFSILPQAFSFWPIV 64 Query: 52 GDV-----------YHIKNYPDFWVPFGWTMGMLSLLVSFLIPFNLM----EKKKLRKQR 96 D+ Y + NY TMG LSL + + L E+++L Sbjct: 65 ADIFSASFDKFTSLYMVANYA--------TMGSLSLYFVLSLAYELTKIYAEEEELNMNP 116 Query: 97 FIAGCTSLITFLIIVTPQVVKDG-------------------TVGFKHSSLGTGGMFVAI 137 +L+ F++ V PQ++ DG +G + GT G+F AI Sbjct: 117 LNGALLALMAFVMTV-PQIIFDGGMMKTVTSLKEGAVIADGWAMGNVVARFGTTGIFTAI 175 Query: 138 IAALITGYIMSLFGKFS-FFKEESVIPDFVRAWFDSMLP---IGVTIILAWVLVDLVHID 193 I A++T I + K + K +P+ V F +++P + +I L+ + D Sbjct: 176 IMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVVAFVVIFINGLLVAMGTD 235 Query: 194 IYNIILNIFMPLASVVETPWGFMLILFISCFLYSMGI-SSWILTPVTKPIFLAAIMANAT 252 I+ +I F ++++ + G M+I ++ L+ +GI + I+ PI LA + NA Sbjct: 236 IFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGANIVFAFVSPIALANMAENA- 294 Query: 253 AGAHNLVTAETIYSAYLWVGGVACTLPLA-FMHLLSKSTKLKALGRASLVPSIFNINEPL 311 AG H V E + ++ GG TL L ++ SKS +LKA+GRAS+VP++FNINEPL Sbjct: 295 AGGHFAVAGE-FSNMFVIAGGSGATLGLCLYIAFASKSEQLKAIGRASVVPALFNINEPL 353 Query: 312 VFG-SIVWNPIMMLPMWINGLVLPLIVWIGTKVIHFGPIPKVVFDMWYVPFPXXXXXXXX 370 +FG I++NP + +P + +V I ++ + PI V W P Sbjct: 354 IFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPIIAQV--PWPTPVGIGAFLGTA 411 Query: 371 XXXXXXXXXXXXXAATLIWYPFFKAYEKQELEKE 404 AA L++ PF + Y+++ +++E Sbjct: 412 DLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEE 445 >VC1282 [G] COG1455 Phosphotransferase system cellobiose-specific component IIC Length = 446 Score = 110 bits (276), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 113/447 (25%), Positives = 195/447 (43%), Gaps = 67/447 (14%) Query: 2 NKLIEWLKNDFSPKMNKVNNNVWVITLKDSVMQLLPFILLGSVFCLLTIP---------- 51 + +I ++ +P KV N V ++D + +PFI++GS + P Sbjct: 8 DAIIGIVEKHIAPIAAKVGNQPHVRAMRDGFIVAMPFIIVGSFILIFAFPPFAEDTTNVF 67 Query: 52 GDV---YHIKNYPDFWVPFGWTMGMLSLLVSFLIPFNLMEKKKLRKQRFIAGCTSLITFL 108 G V + ++ +PF +MG++++ VS + ++L + K+ + SL++FL Sbjct: 68 GRVWLNFATTHFDIIMMPFNMSMGIMTIFVSLGVAYSLAKAYKM--DGITSAVLSLMSFL 125 Query: 109 IIVTPQVVKDGTVGFKHSSLGTGGMFVAIIAALITGYIMSLFGK--FSFFKEESVIPDFV 166 ++ P K+ ++ H +G G+F A++ A + K + E V P Sbjct: 126 LVAAP--AKEDSLSMAH--MGGTGIFTAVMCAFFAVELYRFMKKHNITIRMPEQVPPAIA 181 Query: 167 RAWFDSMLPIGVTIILAWVLVDLVHID----IYNIILNIFMPLASVVETPWGFMLILFIS 222 R+ F+ +LP+ + + L V + I + ++ +F PL S T + L + Sbjct: 182 RS-FEVLLPVLAIFLTLYPLSLFVQAEYGMLIPDAVMAMFKPLISASNTLPAIIGALLVC 240 Query: 223 CFLYSMGI-SSWILTPVTKPIFLAAIMANATAGAHNLVTAETI--------YSAYLWVGG 273 L+ GI + I+ + PIFL I AN A VT + + + Y+++GG Sbjct: 241 QLLWFAGIHGAAIVVGLLSPIFLTNISANIDA----FVTGQPVPNIFTQPFWDFYIFIGG 296 Query: 274 VACTLPLAFMHLLSKSTKLKALGRASLVPSIFNINEPLVFGS-IVWNPIMMLPMWINGLV 332 TL L + S+S LK++GR S VP F INEP++FGS +V NPI+ LP ++ Sbjct: 297 SGATLALVILMSFSRSAHLKSIGRMSAVPGFFQINEPVIFGSPVVMNPILFLPFVFAPVI 356 Query: 333 LPLIVWIGTKVIHFG--------PIPKVVFDMW-----YVPFPXXXXXXXXXXXXXXXXX 379 I + ++ G P ++ W + P Sbjct: 357 NATIAYFAIQLGFVGMGVATTPWTTPAIIGASWGSGWTFTP--------------VLLVV 402 Query: 380 XXXXAATLIWYPFFKAYEKQELEKELP 406 LI+ PFFK +EKQ LE+E P Sbjct: 403 GLLILDLLIYLPFFKMFEKQVLEQEQP 429 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.143 0.463 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,323,900 Number of extensions: 1166087 Number of successful extensions: 3381 Number of sequences better than 1.0e-05: 25 Number of HSP's gapped: 3310 Number of HSP's successfully gapped: 25 Length of query: 409 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 300 Effective length of database: 25,807,025 Effective search space: 7742107500 Effective search space used: 7742107500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 117 (49.7 bits)