BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0122.p (86 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0914 [G] COG1440 Phosphotransferase system cellobiose-specifi... 56 1e-08 >lin0914 [G] COG1440 Phosphotransferase system cellobiose-specific component IIB Length = 301 Score = 56.2 bits (134), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 7/63 (11%), Positives = 17/63 (26%) Query: 20 ARGEAEIENYIDDIDALLVGPHLAYIVDEIEELTRDSDVKVLLMKPSYYSTLDGEAALND 79 +A+ + Y+ + + L+ K + S D E + Sbjct: 36 TSRKAQTTRHRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFV 95 Query: 80 LLS 82 + Sbjct: 96 VEG 98 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.139 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 5,274,567 Number of extensions: 178014 Number of successful extensions: 426 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 426 Number of HSP's successfully gapped: 1 Length of query: 86 Length of database: 38,378,431 Length adjustment: 56 Effective length of query: 30 Effective length of database: 31,919,727 Effective search space: 957591810 Effective search space used: 957591810 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 109 (46.6 bits)