BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0118.p (236 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ydfS [S] COG2323 Predicted membrane protein 231 6e-61 BH1970 [S] COG2323 Predicted membrane protein 149 5e-36 CAC1521 [S] COG2323 Predicted membrane protein 147 2e-35 BS_yetF [S] COG2323 Predicted membrane protein 137 1e-32 CAP0147 [S] COG2323 Predicted membrane protein 137 2e-32 >BS_ydfS [S] COG2323 Predicted membrane protein Length = 235 Score = 231 bits (590), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 116/233 (49%), Positives = 172/233 (73%), Gaps = 3/233 (1%) Query: 5 EILMRTILSVGLLLLIPRILGKQTLSNMTSNDFVISITLGSLAANLAFNVNLKFTYLVLS 64 E+++RT+ S GLLL+ RILGKQT+S MT DF+ +ITLG++AA LA+N ++K + +S Sbjct: 5 EVVIRTVASFGLLLIAERILGKQTISQMTIFDFIAAITLGAIAAGLAYNTSIKPHNMAIS 64 Query: 65 IVVITATSFLLSFIALKSRRLRSWISGSPTVLIEEGKIMEANMRKIRYTMDSLDQALRKK 124 + T FL+SF+++K+R+LR + +G PTVLI+ GKI+E+NMRK+RYT+D L+Q LR+K Sbjct: 65 FSIFVLTIFLISFLSIKNRKLRKFFAGDPTVLIQNGKILESNMRKMRYTLDYLNQQLREK 124 Query: 125 EIFNIEEVDYAVLEDNGQVSVLKKEAYQSVTKQDLGLAL-KPQVFPVELIMDGKLVEDNL 183 EIFNIEEV +A+LE NGQ++VL+K ++ VTKQDL +A+ + Q P+ELIMDG+++E+NL Sbjct: 125 EIFNIEEVLFAILETNGQLTVLRKPQFRHVTKQDLMIAVNQEQRLPIELIMDGEIIENNL 184 Query: 184 KQHGLTREWLEKELDKKQKGKTLADVFYAVKGTQQQLVFDFYEDGLQQPLDRE 236 KQ+ LT WL +EL K+ + + YAV + D Y+D + P+D+E Sbjct: 185 KQNRLTESWLLEELRKRD--IKVKETVYAVLLGNGDIYVDQYKDHISVPMDKE 235 >BH1970 [S] COG2323 Predicted membrane protein Length = 292 Score = 149 bits (375), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 77/224 (34%), Positives = 137/224 (61%), Gaps = 1/224 (0%) Query: 4 AEILMRTILSVGLLLLIPRILGKQTLSNMTSNDFVISITLGSLAANLAFNVNLKFTYLVL 63 + +R ++ +L ++ RI+GK+ +++MT ++++ I +GS+AA L F+ +++ + + Sbjct: 5 GNVFIRGVVGFIVLFILARIMGKKHITDMTYYEYIVGIAIGSIAAELTFSPHVRMSNFLF 64 Query: 64 SIVVITATSFLLSFIALKSRRLRSWISGSPTVLIEEGKIMEANMRKIRYTMDSLDQALRK 123 +++ +LS + LKS R RS G PTVLIE G I+E N++K T+D L LR+ Sbjct: 65 GMIIWAIFPMVLSKLELKSFRFRSLSEGEPTVLIENGNILEENLKKENLTVDELMIHLRQ 124 Query: 124 KEIFNIEEVDYAVLEDNGQVSVLKKEAYQSVTKQDLGLALKPQVFPVELIMDGKLVEDNL 183 K+ F +++V+ AV+E +G +SV+KK Q +T +D GL ++ P +++DG ++E +L Sbjct: 125 KDAFKLDDVESAVMEKSGLISVMKKSNVQPLTPKDAGLVIELDHSPQIVMIDGNVLEKSL 184 Query: 184 KQHGLTREWLEKELDKKQKGKTLADVFYAVKGTQQQLVFDFYED 227 + +G T+EWL E+ KQ K DVF A ++ + D Y D Sbjct: 185 QDYGYTKEWLLAEV-TKQGAKRFEDVFLAQIDSKGNVYVDLYND 227 >CAC1521 [S] COG2323 Predicted membrane protein Length = 242 Score = 147 bits (370), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 78/229 (34%), Positives = 139/229 (60%), Gaps = 1/229 (0%) Query: 6 ILMRTILSVGLLLLIPRILGKQTLSNMTSNDFVISITLGSLAANLAFNVNLKFTYLVLSI 65 +++R+I+ +L+ +ILGKQ +S +T D+++ IT+GS+AA L +++ + + + Sbjct: 7 VIVRSIIGFFTMLIFAKILGKQQISQLTFFDYILGITIGSIAATLTTDLSSRAWPHWVGL 66 Query: 66 VVITATSFLLSFIALKSRRLRSWISGSPTVLIEEGKIMEANMRKIRYTMDSLDQALRKKE 125 + +L+ +I+ K R ++ G PT++I +GKIME M+K+ + + + LR K+ Sbjct: 67 LTWALLGYLMEYISTKWRYAGKYLEGEPTIVIMKGKIMEDAMKKMNFRATDIMELLRDKD 126 Query: 126 IFNIEEVDYAVLEDNGQVSVLKKEAYQSVTKQDLGLALKPQVFPVELIMDGKLVEDNLKQ 185 +F++ EVD+A++E NG++SVLKK Q VT D+ + ELI DG L+E+NL+Q Sbjct: 127 VFDLNEVDFAIIEPNGKLSVLKKPELQPVTCNDMNITKNEMGISTELIYDGILIEENLRQ 186 Query: 186 HGLTREWLEKELDKKQKGKTLADVFYAVKGTQQQLVFDFYEDGLQQPLD 234 T++WL K L+ + K +++VF A L D Y+D +++ LD Sbjct: 187 LNKTKDWLFKVLESRGI-KNVSEVFLATLNPGGSLYIDLYKDHIKKVLD 234 >BS_yetF [S] COG2323 Predicted membrane protein Length = 231 Score = 137 bits (346), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 71/211 (33%), Positives = 124/211 (58%), Gaps = 1/211 (0%) Query: 2 NYAEILMRTILSVGLLLLIPRILGKQTLSNMTSNDFVISITLGSLAANLAFNVNLKFTYL 61 NY + + + +G+L +I ++LGK S +T DF+ ++ LG L N ++ +K + Sbjct: 3 NYLSVAVELVCGLGILFIILKLLGKTQFSQITPFDFISALILGELVGNAVYDHEIKIKEI 62 Query: 62 VLSIVVITATSFLLSFIALKSRRLRSWISGSPTVLIEEGKIMEANMRKIRYTMDSLDQAL 121 + + ++ +++ FI K + R ++ G P ++I +G++ M+K + ++ L L Sbjct: 63 IFASLLWGVLIYIIEFITQKMKSSRKFLEGEPNIVIRKGELQYKVMKKNKIDINQLQSLL 122 Query: 122 RKKEIFNIEEVDYAVLEDNGQVSVLKKEAYQSVTKQDLGLALKPQVFPVELIMDGKLVED 181 R+ F+I+EV+YA+LE NG VSVL K + T +DL + K P+ LI+DG++V D Sbjct: 123 RQAGSFSIQEVEYAILETNGMVSVLPKSDFDKPTNKDLQIPSKSVSLPITLIIDGEIVRD 182 Query: 182 NLKQHGLTREWLEKELDKKQKGKTLADVFYA 212 NLK+ G+ +WL++EL KK KT DV +A Sbjct: 183 NLKEAGVDEQWLKQELKKKNIDKT-EDVLFA 212 >CAP0147 [S] COG2323 Predicted membrane protein Length = 225 Score = 137 bits (344), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 81/221 (36%), Positives = 131/221 (59%), Gaps = 2/221 (0%) Query: 2 NYAEILMRTILSVGLLLLIPRILGKQTLSNMTSNDFVISITLGSLAANLAFNVNLKFTYL 61 +Y + +R+ +S +LLL R +GK+ LS +T D+++ T+GS+AA + + ++ Sbjct: 3 HYMIVFLRSAISYIILLLFTRFMGKKQLSQLTYFDYIVGTTIGSIAAAASVDRHIDVFES 62 Query: 62 VLSIVVITATSFLLSFIALKSRRLRSWISGSPTVLIEEGKIMEANMRKIRYTMDSLDQAL 121 SIVV + + L+S I LKS RLR WI P V+I +G+++ NM K +Y M L L Sbjct: 63 CFSIVVWSVFTILISIITLKSIRLRLWIDSEPLVIINKGRVIYKNMEKAKYNMGDLLMQL 122 Query: 122 RKKEIFNIEEVDYAVLEDNGQVSVLKKEAYQSVTKQDLGLALKPQV-FPVELIMDGKLVE 180 R K+IF I +V+ A+LE +G++SVLKK SVT +DLG+ KP+ VE+I+DG ++ Sbjct: 123 RNKDIFYITDVEIALLEPDGKLSVLKKAEKSSVTVEDLGIQ-KPKTGVMVEIILDGTILY 181 Query: 181 DNLKQHGLTREWLEKELDKKQKGKTLADVFYAVKGTQQQLV 221 ++L+Q W++++L K VF V+ + V Sbjct: 182 NHLQQIKKDEAWVKEQLKIKNIQDIKNVVFLGVQADNEVYV 222 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,640,838 Number of extensions: 605050 Number of successful extensions: 1387 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 1369 Number of HSP's successfully gapped: 16 Length of query: 236 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 133 Effective length of database: 26,499,029 Effective search space: 3524370857 Effective search space used: 3524370857 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)