BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0105.p (334 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0572 [M] COG2222 Predicted phosphosugar isomerases 275 8e-74 STM3601 [M] COG2222 Predicted phosphosugar isomerases 255 5e-68 STM4540 [M] COG2222 Predicted phosphosugar isomerases 155 1e-37 SMc03139 [M] COG2222 Predicted phosphosugar isomerases 147 2e-35 ZyhfN [M] COG2222 Predicted phosphosugar isomerases 126 6e-29 >STM0572 [M] COG2222 Predicted phosphosugar isomerases Length = 328 Score = 275 bits (703), Expect = 8e-74, Method: Compositional matrix adjust. Identities = 135/319 (42%), Positives = 199/319 (62%), Gaps = 3/319 (0%) Query: 17 ILEAFKQKTINNVFYVACGGSSALMYPSKYFIDRESSNITAEIYSSNEFIYRNPKSLGAD 76 I + ++ I V++V CGGS +P KYF+D E+S + +SNEF++ PK+LG + Sbjct: 12 ISDILGKQNIERVWFVGCGGSLTGFWPGKYFLDCEASKLAVGYITSNEFVHATPKALGKN 71 Query: 77 SLVILCSHKGKTPETTDAAHFAKSKGALVVSLMYVDTAPLADESDFIVNYSWAP-PGELD 135 S+VIL S +G T ET AA A+ KGA + L+Y PL + SD+I+ Y WA P +D Sbjct: 72 SVVILASQQGNTAETVAAARVAREKGAATIGLVYQPDTPLCEYSDYIIEYQWARYPETVD 131 Query: 136 PLPEIMNYSILYRLTMGLLYVQEGNELYLKLLSSLENMDTVLEKAKVQYLAPAKAFAAEY 195 P + YS+ L + +L EG Y +L+S+ V+ A+ Q A+ FAAE+ Sbjct: 132 PAQQKAAYSLW--LALEILAQTEGYAQYDELVSAFGRFSDVVHGAQRQVQEDAQRFAAEW 189 Query: 196 KDEKIMYTMASGSNYGIAYSFAICILMEMQWIHSHAIHAGEFFHGPFEILDKNVPFILLM 255 KDEK++Y M SG ++G A+ +ICIL+EMQWI+S +IH+GE+FHGPFEI + PFILL Sbjct: 190 KDEKVVYMMGSGPSFGAAHQESICILLEMQWINSASIHSGEYFHGPFEITEPGTPFILLQ 249 Query: 256 GLDETRPMEERALKFLKEHGEKMLVIDAEQFDLTGIEEEVKGYIAPLVMNLVLRVYAVEL 315 TRP+++RA++F++ + K+ +IDA++ + + +V Y L+ N VL VY + L Sbjct: 250 SSGRTRPLDDRAIRFIERYQGKLQLIDADKLGIQDLSTDVGEYFCGLLHNCVLDVYNLAL 309 Query: 316 AKATGHPLEERRYMFKVPY 334 A A HPL RRYM+KV Y Sbjct: 310 ATARNHPLTTRRYMWKVEY 328 >STM3601 [M] COG2222 Predicted phosphosugar isomerases Length = 325 Score = 255 bits (652), Expect = 5e-68, Method: Compositional matrix adjust. Identities = 132/309 (42%), Positives = 188/309 (60%), Gaps = 4/309 (1%) Query: 26 INNVFYVACGGSSALMYPSKYFIDRESSNITAEIYSSNEFIYRNPKSLGADSLVILCSHK 85 + +V+YVACGGS A YP+K F+++E+ +T +Y+S EFI P +LG +++V++ SHK Sbjct: 21 VKHVYYVACGGSYAAFYPAKAFLEKEAKALTVGLYNSGEFINNPPVALGENAVVVVASHK 80 Query: 86 GKTPETTDAAHFAKSKGALVVSLMYVDTAPLADESDFIVNYSWAPPGELDPLPEIMNYSI 145 G TPET AA A+ GA V+ L ++ +PL D++ Y++ D E + Sbjct: 81 GNTPETIKAAEIARQHGAPVIGLTWIMDSPLVAHCDYVETYTFGDGK--DIAGEKTMKGL 138 Query: 146 LYRLTMGLLYVQEGNELYLKLLSSLENMDTVLEKAKVQYLAPAKAFAAEYKDEKIMYTMA 205 L + LL EG Y + ++ ++ +A Q A+AFA EYKD+K++YT+A Sbjct: 139 LSAVE--LLQQTEGYAHYDDFQDGVSKINRIVWRACEQVAERAQAFAQEYKDDKVIYTVA 196 Query: 206 SGSNYGIAYSFAICILMEMQWIHSHAIHAGEFFHGPFEILDKNVPFILLMGLDETRPMEE 265 SG+ YG AY +ICI MEMQWIHS IH+GEFFHGPFEI D N PF TR ++E Sbjct: 197 SGAGYGAAYLQSICIFMEMQWIHSACIHSGEFFHGPFEITDANTPFFFQFSEGNTRAVDE 256 Query: 266 RALKFLKEHGEKMLVIDAEQFDLTGIEEEVKGYIAPLVMNLVLRVYAVELAKATGHPLEE 325 RAL FLK++G ++ V+DA++ L+ I+ V Y + N V VY LA+A HPL Sbjct: 257 RALNFLKKYGRRIEVVDAKELGLSTIKTTVIDYFNHSLFNNVYPVYNRALAEARQHPLTT 316 Query: 326 RRYMFKVPY 334 RRYM+KV Y Sbjct: 317 RRYMWKVEY 325 >STM4540 [M] COG2222 Predicted phosphosugar isomerases Length = 343 Score = 155 bits (391), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 100/335 (29%), Positives = 170/335 (50%), Gaps = 9/335 (2%) Query: 1 MSTVDRLTKAQFQVERILEAFKQKTINNVFYVACGGSSALMYPSKYFIDRESSNITAEIY 60 +++ + A+ + E++ + Q +++F+ + GGS A M F +E + + + Sbjct: 17 LTSAREIIAARQKAEQVADEIYQAGFSSLFFASVGGSLAPMMAINEFA-KELTTLPVYVE 75 Query: 61 SSNEFIYRNPKSLGADSLVILCSHKGKTPETTDAAHFAKSKGALVVSLMYVDTAPLADES 120 + E I++ K L DS+VI S G T E+ A + K++G VV++ +PLA + Sbjct: 76 QAAELIHKGNKRLNKDSVVITLSKSGDTKESVAIAEWCKAQGIRVVAITKNADSPLAQAA 135 Query: 121 DFIVNYSWAPPGELDPLPEIMNYSILYRLTMGLLYVQEGNELYLKLLSSLENMDTVLEKA 180 +W P E Y +LY L +L Y + S LE + L KA Sbjct: 136 ------TWHIPMRHKNGVE-YEYMLLYWLFFRVLSRNNEFASYDRFASQLEILPANLLKA 188 Query: 181 KVQYLAPAKAFAAEYKDEKIMYTMASGSNYGIAYSFAICILMEMQWIHSHAIHAGEFFHG 240 K ++ A A A+ Y + M + +G Y F++CIL EMQW + + + EFFHG Sbjct: 189 KQKFDPQADAIASRYHNSDYMMWVGGAEMWGEVYLFSMCILEEMQWKRTRPVSSAEFFHG 248 Query: 241 PFEILDKNVPFILLMGLDETRPMEERALKFLKEHGEKMLVIDAEQFDLTGIEEEVKGYIA 300 E+L+K+VP IL+ G + R ++ER +F + + ++VID + + L GI++E + +A Sbjct: 249 ALELLEKDVPLILVKGEGKCRALDERVERFASKITDNLVVIDPKAYALDGIDDEFRWIMA 308 Query: 301 PLVMN-LVLRVYAVELAKATGHPLEERRYMFKVPY 334 P V++ L++ A K TGH L+ RRY + Y Sbjct: 309 PCVVSTLLVDRLAAHFEKYTGHSLDIRRYYRQFDY 343 >SMc03139 [M] COG2222 Predicted phosphosugar isomerases Length = 337 Score = 147 bits (372), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 96/313 (30%), Positives = 152/313 (48%), Gaps = 8/313 (2%) Query: 23 QKTINNVFYVACGGSSALMYPSKYFIDRESSNITAEIYSSNEFIYRNPKSLGADSLVILC 82 +K NVF++ GG++ LM P+ + R S A I E +L S+V++ Sbjct: 32 EKGAENVFFLGTGGAAILMQPAAQLLQRRS-RFPAFIDLPAELFITGSVNLTEKSIVVIP 90 Query: 83 SHKGKTPETTDAAHFAKSKGALVVSLMYVDTAPLADESDF-IVNYSWAPPGELDPLPEIM 141 S G T E+ AK GA V+SL+ + PL D VN++ E D E Sbjct: 91 SLSGTTKESVALLAKAKEAGATVLSLVGHEETPLGKGGDHAFVNFA-----EDDTSCESF 145 Query: 142 NYSILYRLTMGLLYVQEGNELYLKLLSSLENMDTVLEKAKVQYLAPAKAFAAEYKDEKIM 201 L+ + + ++ + E Y ++ LE + +L + K Y A+ FA Sbjct: 146 YLQSLF-IALSIMRHRGEIENYDQIAGELERLPGLLLEVKRGYEDKAETFARVLAGSDYH 204 Query: 202 YTMASGSNYGIAYSFAICILMEMQWIHSHAIHAGEFFHGPFEILDKNVPFILLMGLDETR 261 +G+ + A+ + +CIL EMQWI + +HA +FFHG E+++K V IL G D R Sbjct: 205 IVTGAGNAWPEAFYYGMCILEEMQWIRTRPVHASDFFHGTLELVEKGVSVILFKGEDALR 264 Query: 262 PMEERALKFLKEHGEKMLVIDAEQFDLTGIEEEVKGYIAPLVMNLVLRVYAVELAKATGH 321 P+ +R F + +++ V+D F L GI +EV+G ++P+V+ VL + L H Sbjct: 265 PLADRVQNFAPNYTDRLTVLDTADFALPGISQEVRGLVSPIVLATVLERISAHLEVMRNH 324 Query: 322 PLEERRYMFKVPY 334 PL RRY +V Y Sbjct: 325 PLTTRRYYKRVAY 337 >ZyhfN [M] COG2222 Predicted phosphosugar isomerases Length = 347 Score = 126 bits (316), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 87/318 (27%), Positives = 145/318 (45%), Gaps = 19/318 (5%) Query: 14 VERILEAFKQKTINNVFYVACGGSSALMYPSKYFIDRESSNITAEIYSSNEFIYRNPKSL 73 V I+E ++ I+ +++VACG +K+ DR S++ S EF P L Sbjct: 39 VHAIVEEMVKRDIDRIYFVACGSPLNAAQTAKHLADR-FSDLQVYAISGWEFCDNTPYRL 97 Query: 74 GADSLVILCSHKGKTPETTDAAHFAKSKGALVVSLMYVDTAPLADESDFIVNYSWAPPGE 133 VI S GKT E A ++ GAL + +P+ ++F ++Y + Sbjct: 98 DDRCAVIGVSDYGKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDY------Q 151 Query: 134 LDPLPEI---MNYSILYRLTMGLLYVQEGNELYLKLLSSLENMDTVLEKAKVQYLAPAKA 190 D + EI + YS++ + L E K+ + L+ + L + + Sbjct: 152 ADCIWEIHLLLCYSVVLEMITRLAPNAEIG----KIKNDLKQLPNALGHLVRTWEEKGRQ 207 Query: 191 FAAEYKDEKIMYTMASGSNYGIAYSFAICILMEMQWIHSHAIHAGEFFHGPFEILDKNVP 250 ++YT+A+G + Y I LME W H I +GEF HGP EI++ VP Sbjct: 208 LGELASQWPMIYTVAAGPLRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVP 267 Query: 251 FILLMGLDETRPMEERALKFLKEHGEKMLVIDAEQFDLTGIEEEVKGYIAPLVMNLVLRV 310 F+ L+G DE+R ERA+ F+K+ + ++VI D I + + ++AP +M + + Sbjct: 268 FLFLLGNDESRHTTERAINFVKQRTDNVIVI-----DYAEISQGLHPWLAPFLMFVPMEW 322 Query: 311 YAVELAKATGHPLEERRY 328 L+ H +ERRY Sbjct: 323 LCYYLSIYKDHNPDERRY 340 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,991,807 Number of extensions: 985224 Number of successful extensions: 2568 Number of sequences better than 1.0e-05: 23 Number of HSP's gapped: 2543 Number of HSP's successfully gapped: 23 Length of query: 334 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 227 Effective length of database: 26,037,693 Effective search space: 5910556311 Effective search space used: 5910556311 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)