BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0104.p (469 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0274 [E] COG1027 Aspartate ammonia-lyase 535 e-152 CAC1652 [E] COG1027 Aspartate ammonia-lyase 419 e-117 PA0854 [C] COG0114 Fumarase 364 e-100 NMA1001 [G] COG1109 Phosphomannomutase 355 1e-97 STM0444 [GEPR] COG0477 Permeases of the major facilitator superf... 345 6e-95 >CAC0274 [E] COG1027 Aspartate ammonia-lyase Length = 465 Score = 535 bits (1377), Expect = e-152, Method: Compositional matrix adjust. Identities = 252/461 (54%), Positives = 337/461 (73%) Query: 6 RVEHDFLGEMEVPADAYYGIQTLRAIENFPITGYSIHERLITSMAMVKKAAALANMDIHR 65 R+E D +G+ +VP + YYG+QTLR +NF ITG ++E I S+ +KKAAA+ N++I Sbjct: 4 RIESDSIGDKKVPKEVYYGVQTLRGADNFKITGLRLNEEFIKSVVQIKKAAAITNLEIGE 63 Query: 66 LPSRIGHVIVQAADEIIAGGWSEQFKVDPIQGGAGTSINMNANEVIANRAIELLGGVKGD 125 L IG I++A DE+IAG ++E F +DPIQGGAGTSINMNANEVIANRAIE+LG KG+ Sbjct: 64 LEVNIGKAIIKACDEVIAGKFNEDFIIDPIQGGAGTSINMNANEVIANRAIEILGYEKGN 123 Query: 126 YTMISPNSHVNMSQSTNDSFPTATHIAVLYLIDELLVTMAGLHGAFVRKAEEFDGIIKMG 185 Y ++ PN HVNM QSTND PTA I L L+ + L + L AF KAE+F +IKMG Sbjct: 124 YRIVHPNDHVNMGQSTNDVIPTAGKITALRLVIKNLDKLKELSNAFSEKAEQFKDVIKMG 183 Query: 186 RTHLQDAVPIRLGQEFAAYARMLERDLVRIRQTKFHLYEINMGATAVGTGLNADPRYISK 245 RT LQDAVPI LGQEF+AY+ +++RD+ RI++ + L +N+G TAVGTG+NAD RY+ + Sbjct: 184 RTQLQDAVPITLGQEFSAYSAVVKRDIARIQKAQDELKVVNIGGTAVGTGINADSRYVDR 243 Query: 246 VVELLADISGYPLENAAHLPDATQNTDVYTEVSAALKIAMINMSKAANDLRLMASGPRAG 305 +V L+ +SG L+ A L D TQN D + +SA +K +N+SK ANDLRLM+SGPR G Sbjct: 244 IVPRLSFVSGLNLKKADDLIDGTQNLDSFASLSATIKTCALNLSKIANDLRLMSSGPRTG 303 Query: 306 LGEIKLPARQPGSSIMPGKVNPVMCEVVNQVAFQVMGNDHTICMASEAGQLELNVMEPVL 365 LGEI LPA+Q GSSIMPGKVNPV+ EV+NQ+AF + GND I MA EAGQLELN EPV+ Sbjct: 304 LGEINLPAKQNGSSIMPGKVNPVIPEVINQIAFNIAGNDVAISMAVEAGQLELNAFEPVI 363 Query: 366 VFNLLQSLSIMNRALGAFQQHCIDGITVDKERCEGYVKQSVGVITALNPYLGYEEAARIA 425 +N+ +S+ + + F ++CI IT ++E C YV SVG++TAL PY+GY E+A+IA Sbjct: 364 FYNIFESIQTLTNGVDTFIKNCILDITANEEICSEYVNNSVGIVTALCPYIGYAESAKIA 423 Query: 426 HEAIQTGESVRSLCLRYNLLKEEELDRILNPYRMTEPGIAG 466 AI++GES++ + LR +LKE+EL+ I+NP +MT+PGI G Sbjct: 424 KMAIKSGESIKKVVLREGVLKEKELNSIINPLKMTKPGIVG 464 >CAC1652 [E] COG1027 Aspartate ammonia-lyase Length = 470 Score = 419 bits (1077), Expect = e-117, Method: Compositional matrix adjust. Identities = 214/461 (46%), Positives = 307/461 (66%) Query: 6 RVEHDFLGEMEVPADAYYGIQTLRAIENFPITGYSIHERLITSMAMVKKAAALANMDIHR 65 R+E D LGE +P ++YYGI +LRA ENF I+ ++ LI ++ +VKKAAALA+ + Sbjct: 9 RIEKDLLGEKNLPKESYYGINSLRAFENFNISNKKVNLNLIYAVVIVKKAAALAHKKLKE 68 Query: 66 LPSRIGHVIVQAADEIIAGGWSEQFKVDPIQGGAGTSINMNANEVIANRAIELLGGVKGD 125 L I+ A D+I++G + ++F + +QGGAGTS NMN NEVIANRAIELL G KGD Sbjct: 69 LKDEKADAIISACDDILSGKFDDEFITNSLQGGAGTSTNMNVNEVIANRAIELLNGSKGD 128 Query: 126 YTMISPNSHVNMSQSTNDSFPTATHIAVLYLIDELLVTMAGLHGAFVRKAEEFDGIIKMG 185 Y++I P VNMSQSTND +PTA IA + L+ L ++ L +F K +F I+ +G Sbjct: 129 YSIIHPIEDVNMSQSTNDVYPTALRIAAIKLLRPLSDALSKLQESFQIKENDFSDILMLG 188 Query: 186 RTHLQDAVPIRLGQEFAAYARMLERDLVRIRQTKFHLYEINMGATAVGTGLNADPRYISK 245 RT L DA+P+ +GQ F AYA+ RD RI + + L EIN+G A+GTG+NA YI Sbjct: 189 RTELMDALPMMVGQGFGAYAKAFARDRWRIYKVEERLREINIGGNAIGTGINASYDYIFT 248 Query: 246 VVELLADISGYPLENAAHLPDATQNTDVYTEVSAALKIAMINMSKAANDLRLMASGPRAG 305 V ++L D++G L + + D TQN DV+ EVS LK +N+ K +ND+RL+ SGP G Sbjct: 249 VTDILQDLTGLGLARSDYPMDVTQNMDVFVEVSGLLKSCAVNLIKISNDIRLLNSGPVGG 308 Query: 306 LGEIKLPARQPGSSIMPGKVNPVMCEVVNQVAFQVMGNDHTICMASEAGQLELNVMEPVL 365 +GE + Q GSSIMPGKVNPV+CE+ Q + +VM ND I +AS GQL+LN P++ Sbjct: 309 IGEFIVEEVQAGSSIMPGKVNPVICEMTAQTSMKVMANDTAISLASSFGQLQLNAFSPLI 368 Query: 366 VFNLLQSLSIMNRALGAFQQHCIDGITVDKERCEGYVKQSVGVITALNPYLGYEEAARIA 425 +LL+SL I+ A+ F++ CID I +++E+C ++ S ++TAL Y+GY++A+ IA Sbjct: 369 AESLLESLEILTNAVNVFREKCIDSIKINEEKCLENLEASTALVTALVHYIGYDKASIIA 428 Query: 426 HEAIQTGESVRSLCLRYNLLKEEELDRILNPYRMTEPGIAG 466 +A++ +S+R L L +L +EE+D+ILNPY +T+PGI G Sbjct: 429 KKALKEKKSIRELLLLEKILTKEEIDKILNPYEITKPGIPG 469 >PA0854 [C] COG0114 Fumarase Length = 464 Score = 364 bits (934), Expect = e-100, Method: Compositional matrix adjust. Identities = 202/464 (43%), Positives = 282/464 (60%), Gaps = 1/464 (0%) Query: 4 LVRVEHDFLGEMEVPADAYYGIQTLRAIENFPITGYSIHERLITSMAMVKKAAALANMDI 63 + R E D LG +EVP DAY+G QT R++ENF I G + +I ++A++KKAAA N + Sbjct: 1 MSRTETDSLGTIEVPDDAYWGAQTQRSLENFAIGGQRMPLAVIHALALIKKAAARVNDHL 60 Query: 64 HRLPSRIGHVIVQAADEIIAGGWSEQFKVDPIQGGAGTSINMNANEVIANRAIELLGGVK 123 LP + +I QAADE++AG E F + Q G+GT NMN NEVIA RA EL G + Sbjct: 61 GELPPEVARLIEQAADEVLAGRHDEHFPLVVWQTGSGTQTNMNVNEVIAGRANELAGNPR 120 Query: 124 GDYTMISPNSHVNMSQSTNDSFPTATHIAVLYLIDE-LLVTMAGLHGAFVRKAEEFDGII 182 G + + PN HVN +QS+NDSFPTA HIA + E LL +A L G ++ ++ Sbjct: 121 GGKSPVHPNDHVNRAQSSNDSFPTAMHIAAAKAVHEQLLPAIAELSGGLAEQSARHASLV 180 Query: 183 KMGRTHLQDAVPIRLGQEFAAYARMLERDLVRIRQTKFHLYEINMGATAVGTGLNADPRY 242 K GRTHL DA PI GQE +A+ L+ IR +Y++ G TAVGTGLNA + Sbjct: 181 KTGRTHLMDATPITFGQELSAFVAQLDYAERAIRAALPAVYQLAQGGTAVGTGLNAPKGF 240 Query: 243 ISKVVELLADISGYPLENAAHLPDATQNTDVYTEVSAALKIAMINMSKAANDLRLMASGP 302 + +A SG P A + A + +S ALK + + K ANDLRL+ SGP Sbjct: 241 ADAIAAEIAAESGLPFVAAPNKFAALAGHEPLVILSGALKSLAVALMKIANDLRLLGSGP 300 Query: 303 RAGLGEIKLPARQPGSSIMPGKVNPVMCEVVNQVAFQVMGNDHTICMASEAGQLELNVME 362 RAG E+KLPA +PGSSIMPGKVNP CE ++ +A QVMGND TI A+ G L+LNV + Sbjct: 301 RAGFAEVKLPANEPGSSIMPGKVNPTQCEALSMLACQVMGNDSTISFAASQGHLQLNVFK 360 Query: 363 PVLVFNLLQSLSIMNRALGAFQQHCIDGITVDKERCEGYVKQSVGVITALNPYLGYEEAA 422 PV+V+NLL+S+ ++ F +HC+ G+ D +R +++ + ++TALNP++GY++AA Sbjct: 361 PVIVYNLLESIRLLADGCRNFNKHCVAGLEPDAQRMADLLERGLMLVTALNPHIGYDKAA 420 Query: 423 RIAHEAIQTGESVRSLCLRYNLLKEEELDRILNPYRMTEPGIAG 466 IA +A G ++R+ L+ L E + D + P +M E G G Sbjct: 421 EIAKKAYAEGTTLRAAALQLGYLDEAQFDEWVRPEQMLEAGHHG 464 >NMA1001 [G] COG1109 Phosphomannomutase Length = 346 Score = 355 bits (910), Expect = 1e-97, Method: Compositional matrix adjust. Identities = 0/455 (0%), Positives = 0/455 (0%), Gaps = 1/455 (0%) Query: 6 RVEHDFLGEMEVPADAYYGIQTLRAIENFPITGYSIHERLITSMAMVKKAAALANMDIHR 65 + + + G + + + + Sbjct: 31 DAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSG 90 Query: 66 LPSRIGHVIVQAADEIIAGGWSEQFKVDPIQGGAGTSINMNANEVIANRAIELLGGVKGD 125 I + LG D Sbjct: 91 PSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTD 150 Query: 126 YTMISPNSHVNMSQSTNDSFPTATHIA-VLYLIDELLVTMAGLHGAFVRKAEEFDGIIKM 184 + + +I ++ + + + + Sbjct: 151 SAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSL 210 Query: 185 GRTHLQDAVPIRLGQEFAAYARMLERDLVRIRQTKFHLYEINMGATAVGTGLNADPRYIS 244 + + E + + + + + + Sbjct: 211 LNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQT 270 Query: 245 KVVELLADISGYPLENAAHLPDATQNTDVYTEVSAALKIAMINMSKAANDLRLMASGPRA 304 + S L ++ K + + Sbjct: 271 QKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDV 330 Query: 305 GLGEIKLPARQPGSSIMPGKVNPVMCEVVNQVAFQVMGNDHTICMASEAGQLELNVMEPV 364 Sbjct: 331 390 Query: 365 LVFNLLQSLSIMNRALGAFQQHCIDGITVDKERCEGYVKQSVGVITALNPYLGYEEAARI 424 Sbjct: 391 450 Query: 425 AHEAIQTGESVRSLCLRYNLLKEEELDRILNPYRM 459 Sbjct: 451 485 >STM0444 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 491 Score = 345 bits (886), Expect = 6e-95, Method: Compositional matrix adjust. Identities = 25/465 (5%), Positives = 74/465 (15%), Gaps = 16/465 (3%) Query: 5 VRVEHDFLGEMEVPADAYYGIQTLRAIENFPITGYSIHERLITSMAMVKKAAALANMDIH 64 + ++ + + A + +L Sbjct: 1 MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENIDLKTIGFFSLVGQAYV 60 Query: 65 RLPSRIGHVIVQAADEIIAGGWSEQFK---VDPIQGGAGTSINMNANEVIANRAIELLGG 121 + + + A + R + L Sbjct: 61 FKFLWSPLMDRYTPPF-----LGRRRGWLLATQLLLLAAIAAMGFLEPGTQLRWMAALAV 115 Query: 122 VKGDYTMISPNSHVNMSQSTNDSFPTATHIAVLYLIDELLVTMAGLHGAFVRKAEEFDGI 181 V S + + ++ A + ++ L + A Sbjct: 116 VIAFC-SASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSGGLALWLADRWLGWQ 174 Query: 182 IKMGRTHLQDAVPIRLGQEFAAYARMLERDLVRIRQTKFHLYEINMGATAVGTGLNADPR 241 I + + L + A L Sbjct: 175 GMYWLMAALLIPCIIATLLAPEPTDTIPSPKSLEQAVVAPLRDFFGRNNAWLILLLIVLY 234 Query: 242 YISKVVELLADISGYPLENAAHLPDATQNTDVYTEVSAALKIAMINMSKAANDLRLMASG 301 + + + + ++ + + L Sbjct: 235 KLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGALYGGILMQRLSLFRALLI 294 Query: 302 PRAGLGEIKLPARQPGSSIMPGKVNPVMCEVVNQVAFQVMGNDHTICMASEAGQLELNVM 361 G + N + M Sbjct: 295 FGILQGASNAGYWLLSITDKNMFSMGAAVFFENLCGGMGTAAFVALLMTLCNKSFSATQF 354 Query: 362 EPVLVFNLLQSLSIMNRALGAFQQHCIDGITVDKERCEGYVKQSVGVITALNPYLGYE-- 419 + + + + + A + H + ++ ++ Sbjct: 355 ALLSALSAVGRVYVGPVAGWFVEAH-GWPTFYLFSVVAAVPGLLLLLVCRQTLEYSWQSE 413 Query: 420 -EAARIAHEAIQTGESVRSLCLRYNLLKEEELDRILNPYRMTEPG 463 R + S+ L L + +L + Sbjct: 414 RFIPRTQYRGAY--NFALSILLAGVALLAVWV-LLLTMNAVDYTN 455 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,384,266 Number of extensions: 1363028 Number of successful extensions: 3339 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 3328 Number of HSP's successfully gapped: 20 Length of query: 469 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 359 Effective length of database: 25,691,691 Effective search space: 9223317069 Effective search space used: 9223317069 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)