BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0103.p (395 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ytsJ [C] COG0281 Malic enzyme 357 2e-98 BH3168 [C] COG0281 Malic enzyme 351 1e-96 BH0399 [C] COG0281 Malic enzyme 343 4e-94 MA1735 [C] COG0281 Malic enzyme 331 1e-90 CC3549_1 [C] COG0281 Malic enzyme 318 1e-86 >BS_ytsJ [C] COG0281 Malic enzyme Length = 410 Score = 357 bits (917), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 195/376 (51%), Positives = 260/376 (69%), Gaps = 2/376 (0%) Query: 15 GKIHTRPNLPILNHDDLARIYTPGVAQVCTAIHEDPEQVYALTVKRNMVAVVTDGTAVLG 74 GK+ ++ + + N DL+ Y+PGVA+ C IHED +VY T+K NMVAVVTDGTAVLG Sbjct: 16 GKLESKSKVEVRNAKDLSLAYSPGVAEPCKDIHEDINKVYDYTMKGNMVAVVTDGTAVLG 75 Query: 75 LGNIGPYAAAPVMEGKAVLFKQLAGINAFPICLDTTDTEEIIRTVKAIAPIFGGINLEDI 134 LGNIGP AA PVMEGKAVLFK AG++AFPI L+T D ++I+ TVK + P FGG+NLEDI Sbjct: 76 LGNIGPEAALPVMEGKAVLFKSFAGVDAFPIALNTNDVDKIVETVKLLEPTFGGVNLEDI 135 Query: 135 SSPRCFXXXXXXXXXXXXPVFHDDQHGTAIVVLAGLINALKVTGKQIDHVRIVVNGVGAA 194 ++P CF PVFHDDQHGTAIV +AGL+NALK++GK + +++V NG GAA Sbjct: 136 AAPNCFIIEERLKKETNIPVFHDDQHGTAIVTVAGLVNALKLSGKSMSSIKVVANGAGAA 195 Query: 195 GVSICRMLLAAGARHLVPVDQEGALIRGG-NYAHPKWQWLAGQSGVEDQPGTLQEVIAGA 253 G++I ++L G R +V D +GA+ G N + +A + + + G+L++VI A Sbjct: 196 GIAIIKLLHHYGVRDIVMCDSKGAIYEGRPNGMNDVKNEVAKFTNQDRKDGSLKDVIVDA 255 Query: 254 DVFIGVSRGGILQPQDVQRMAPLPIVFAMANPEPEISPELALPY-AGVYATGRSDYPNQI 312 DVFIGVS G L + VQ MA PI+FAMANP PEI PE A A V TGRSD+PNQ+ Sbjct: 256 DVFIGVSVAGALTKEMVQSMAKDPIIFAMANPNPEIMPEDAREAGASVVGTGRSDFPNQV 315 Query: 313 NNVLAFPGIFKGALECRAQRINDQMKLXXXXXXXXVVKDTELNAAYIIPSLFNDQVVIKV 372 NNVLAFPGIF+GAL+ RA IN++MK+ +V + EL+A Y+IP+ F+ +V V Sbjct: 316 NNVLAFPGIFRGALDVRATHINEEMKIAAVEAIASLVSEDELSADYVIPAPFDKRVAPAV 375 Query: 373 RQAVIEAAVSSGIAKI 388 +AV +AA+ +G+A+I Sbjct: 376 AKAVAKAAMETGVARI 391 >BH3168 [C] COG0281 Malic enzyme Length = 410 Score = 351 bits (901), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 189/375 (50%), Positives = 254/375 (67%), Gaps = 2/375 (0%) Query: 15 GKIHTRPNLPILNHDDLARIYTPGVAQVCTAIHEDPEQVYALTVKRNMVAVVTDGTAVLG 74 GK+ ++ +P+ N DL+ Y+PGVA+ C I ED E VY T+K NMVAVV++GTAVLG Sbjct: 17 GKLESKSKVPVRNARDLSLAYSPGVAEPCKEIFEDVETVYEYTMKGNMVAVVSNGTAVLG 76 Query: 75 LGNIGPYAAAPVMEGKAVLFKQLAGINAFPICLDTTDTEEIIRTVKAIAPIFGGINLEDI 134 LGNIGP A+ PVMEGKAVLFK AG++AFPICL TTD ++I+ TVK + P FGG+NLEDI Sbjct: 77 LGNIGPEASLPVMEGKAVLFKSFAGVDAFPICLRTTDVDQIVETVKLLEPTFGGVNLEDI 136 Query: 135 SSPRCFXXXXXXXXXXXXPVFHDDQHGTAIVVLAGLINALKVTGKQIDHVRIVVNGVGAA 194 ++P CF PVFHDDQHGTAIV LAGLINALK+TGK++ +++V NG GAA Sbjct: 137 AAPSCFEIEERLKKETNIPVFHDDQHGTAIVTLAGLINALKLTGKRMSEIKVVANGAGAA 196 Query: 195 GVSICRMLLAAGARHLVPVDQEGALIRGGNYAHPKWQWLAGQSGVEDQ-PGTLQEVIAGA 253 G++I +++ G R ++ D +GA+ G Y + + D+ G L +V+ GA Sbjct: 197 GIAIIKLMQRMGVRDIIMCDSKGAIYDGRPYGMNDMKAEVAKWTNRDKIDGGLGDVLKGA 256 Query: 254 DVFIGVSRGGILQPQDVQRMAPLPIVFAMANPEPEISPELALPY-AGVYATGRSDYPNQI 312 DVFIGVS G L + ++ M PI+FAMANP PEI PE A A V TGRSD+PNQ+ Sbjct: 257 DVFIGVSVAGALTKEMIENMNDDPIIFAMANPVPEIMPEEAKAAGAKVIGTGRSDFPNQV 316 Query: 313 NNVLAFPGIFKGALECRAQRINDQMKLXXXXXXXXVVKDTELNAAYIIPSLFNDQVVIKV 372 NNVLAFPGIF+GAL+ RA IN++MK+ +V + +L+ Y+IP+ F+ +V V Sbjct: 317 NNVLAFPGIFRGALDARATHINEEMKVAAVHAIADLVAEEDLDPDYVIPAPFDPRVAPAV 376 Query: 373 RQAVIEAAVSSGIAK 387 AV + A+ +G+A+ Sbjct: 377 AAAVAKTAMETGVAR 391 >BH0399 [C] COG0281 Malic enzyme Length = 397 Score = 343 bits (879), Expect = 4e-94, Method: Compositional matrix adjust. Identities = 188/373 (50%), Positives = 248/373 (66%), Gaps = 3/373 (0%) Query: 12 HQ--GGKIHTRPNLPILNHDDLARIYTPGVAQVCTAIHEDPEQVYALTVKRNMVAVVTDG 69 HQ GK+ + I + DL+ +YTPGVA VC I E P++V +LT + NMVA+VTDG Sbjct: 14 HQKHSGKLEIVGKMEITDEKDLSLVYTPGVADVCKQIAEQPDKVNSLTSRGNMVAIVTDG 73 Query: 70 TAVLGLGNIGPYAAAPVMEGKAVLFKQLAGINAFPICLDTTDTEEIIRTVKAIAPIFGGI 129 TAVLGLGNIGP AA PVMEGKA+LFK+ A I+ FP+CL+T DT+EI++ +KA++P F GI Sbjct: 74 TAVLGLGNIGPKAAMPVMEGKALLFKKFANIDGFPVCLNTQDTDEIVQIIKALSPTFAGI 133 Query: 130 NLEDISSPRCFXXXXXXXXXXXXPVFHDDQHGTAIVVLAGLINALKVTGKQIDHVRIVVN 189 NLEDI++PRCF PVFHDDQHGTAIVVLA L NALKV K +++V+N Sbjct: 134 NLEDIAAPRCFEIEERLKEELDIPVFHDDQHGTAIVVLAALTNALKVVNKLNQPIKMVIN 193 Query: 190 GVGAAGVSICRMLLAAGARHLVPVDQEGALIRGGNYAHPKWQWLAGQSGVEDQPGTLQEV 249 G GAAG +I +LL AG + + V EG + +G + +P +A + + GTL++ Sbjct: 194 GAGAAGTAIANLLLHAGFKDITLVSLEGVVAKGEAWLNPMQAKMAEVTNLAGVKGTLKDA 253 Query: 250 IAGADVFIGVSRGGILQPQDVQRMAPLPIVFAMANPEPEISPELALPY-AGVYATGRSDY 308 I ADVFIGVS G ++ + +Q MA PIV A+ANP PEI PE A+ A V TGRSDY Sbjct: 254 ITDADVFIGVSGPGAMKKEHIQAMAKDPIVLALANPIPEIYPEEAIEAGAAVVGTGRSDY 313 Query: 309 PNQINNVLAFPGIFKGALECRAQRINDQMKLXXXXXXXXVVKDTELNAAYIIPSLFNDQV 368 PNQ+NN+LAFPGIF+GAL+ A+ I +MK+ V+ D E+NA IIP + +V Sbjct: 314 PNQVNNLLAFPGIFRGALDAGAKTITMEMKIAAAYAIADVLSDKEVNANMIIPDALDARV 373 Query: 369 VIKVRQAVIEAAV 381 +V +AV A+ Sbjct: 374 AQRVAEAVATTAL 386 >MA1735 [C] COG0281 Malic enzyme Length = 390 Score = 331 bits (848), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 177/353 (50%), Positives = 230/353 (65%), Gaps = 4/353 (1%) Query: 14 GGKIHTRPNLPILNHDDLARIYTPGVAQVCTAIHEDPEQVYALTVKRNMVAVVTDGTAVL 73 GG + + + DL+ YTPGVA+ C I E+P VY T+K+N VAVVTDG+AVL Sbjct: 6 GGVLEVESKVRLRTIHDLSVAYTPGVAEPCRKIKENPSLVYLYTIKKNTVAVVTDGSAVL 65 Query: 74 GLGNIGPYAAAPVMEGKAVLFKQLAGINAFPICLDTTDTEEIIRTVKAIAPIFGGINLED 133 GLGNIGP AA PVMEGKA++FK+ AGI+AFPICL+T DTEE+I+ VK +AP FGGINLED Sbjct: 66 GLGNIGPCAALPVMEGKAIIFKEFAGIDAFPICLNTQDTEEVIKAVKYLAPAFGGINLED 125 Query: 134 ISSPRCFXXXXXXXXXXXXPVFHDDQHGTAIVVLAGLINALKVTGKQIDHVRIVVNGVGA 193 IS+PRCF PV HDDQHG AIVV AGL+NALK+ K++ ++I++ G+GA Sbjct: 126 ISAPRCFEIESRLREELDIPVIHDDQHGAAIVVFAGLLNALKIVKKELGELKILITGLGA 185 Query: 194 AGVSICRMLLAAGAR--HLVPVDQEGALIRGGNY-AHPKWQWLAGQSGVEDQPGTLQEVI 250 AGV+I R L+ AGA ++ D +G + G +P +A + G L+E Sbjct: 186 AGVAIFRFLVRAGADPAKILTCDSQGIVYEGREKDMNPIKAEIATLTNPGKLKGGLKEAF 245 Query: 251 AGADVFIGVSRGGILQPQDVQRMAPLPIVFAMANPEPEISPELALPY-AGVYATGRSDYP 309 G D+FIGVS GGI+ + + MA IV AMANP PEI P+ A A + ATGRSD+P Sbjct: 246 PGTDLFIGVSVGGIVTEEMISSMANDAIVMAMANPTPEIMPDAAKRAGARIVATGRSDFP 305 Query: 310 NQINNVLAFPGIFKGALECRAQRINDQMKLXXXXXXXXVVKDTELNAAYIIPS 362 NQ+NN L+FPG+FKGAL A++I +M++ VV EL+ YIIP Sbjct: 306 NQLNNCLSFPGVFKGALGTCARKITPEMEMAAAHALANVVSVGELSEDYIIPE 358 >CC3549_1 [C] COG0281 Malic enzyme Length = 453 Score = 318 bits (814), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 173/391 (44%), Positives = 239/391 (61%), Gaps = 21/391 (5%) Query: 15 GKIHTRPNLPILNHDDLARIYTPGVAQVCTAIHEDPEQVYALTVKRNMVAVVTDGTAVLG 74 GKI P P+ DL+ Y+PGVA AI DP+ Y T K N+VAV+++GTA+LG Sbjct: 29 GKIAIVPTKPMATQRDLSLAYSPGVAVPVHAIAADPDMAYEYTSKGNLVAVISNGTAILG 88 Query: 75 LGNIGPYAAAPVMEGKAVLFKQLAGINAFPICLDTTDTEEIIRTVKAIAPIFGGINLEDI 134 LG++G A+ PVMEGK+VLFK+ +++ I + T D +EII VK I FGGINLEDI Sbjct: 89 LGDLGALASKPVMEGKSVLFKRFGDVDSIDIEVTTKDPDEIITVVKNIGVTFGGINLEDI 148 Query: 135 SSPRCFXXXXXXXXXXXXPVFHDDQHGTAIVVLAGLINALKVTGKQIDHVRIVVNGVGAA 194 SP CF PVFHDDQHGTAI+ AGLINA +TGK+I+ V++V+NG GAA Sbjct: 149 KSPECFRIETELQELLDIPVFHDDQHGTAIICAAGLINACHITGKKIEDVKVVLNGPGAA 208 Query: 195 GVSICRMLLAAGAR--HLVPVDQEGALIRGGNYAHPKWQWLAGQSGVEDQPGTLQEVIAG 252 G++ ++ A G R + + VD +G L RG +W+ VE TL E + G Sbjct: 209 GIASLELIKAMGVRAENCIAVDSKGVLYRGRTEGMNQWK---SAHAVETDKRTLAEAVVG 265 Query: 253 ADVFIGVSRGGILQPQDVQRMAPLPIVFAMANPEPEISPELA--LPYAGVYATGRSDYPN 310 ADV +G+S G P+ + MAP P++FAMANP+PEI+PE + + TGRSDYPN Sbjct: 266 ADVLLGLSAKGAFTPEMIASMAPNPVIFAMANPDPEITPEEVKRVRKDAIMGTGRSDYPN 325 Query: 311 QINNVLAFPGIFKGALECRAQRINDQMKLXXXXXXXXVVKD---TELNAA---------- 357 Q+NNVL FP IF+GAL+ RA+R+N +MK+ + ++ E+ AA Sbjct: 326 QVNNVLGFPYIFRGALDVRARRVNHEMKIACAQALAMLAREDVPDEVAAAYHGRQLKFGP 385 Query: 358 -YIIPSLFNDQVVIKVRQAVIEAAVSSGIAK 387 YIIPS F+ +++ V V +AA+ +G+A+ Sbjct: 386 DYIIPSAFDPRLIWYVPPFVAQAAMDTGVAR 416 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.140 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,239,560 Number of extensions: 1188906 Number of successful extensions: 2426 Number of sequences better than 1.0e-05: 36 Number of HSP's gapped: 2342 Number of HSP's successfully gapped: 36 Length of query: 395 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 287 Effective length of database: 25,922,359 Effective search space: 7439717033 Effective search space used: 7439717033 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 117 (49.7 bits)