BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0102.p (400 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value HI0308 [GEPR] COG0477 Permeases of the major facilitator superfa... 84 3e-16 TM1032 [GEPR] COG0477 Permeases of the major facilitator superfa... 79 2e-14 STM1032 [R] COG5511 Bacteriophage capsid protein 79 2e-14 VC0302 [GEPR] COG0477 Permeases of the major facilitator superfa... 78 2e-14 ECs0396 [GEPR] COG0477 Permeases of the major facilitator superf... 59 1e-08 >HI0308 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 388 Score = 84.3 bits (207), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 93/352 (26%), Positives = 152/352 (43%), Gaps = 30/352 (8%) Query: 17 FYIAYASYLPFISYWFAEDGLSTQQIGLIFSIGPLVGFLVQPLWGMLIDYYGMAKWLLLI 76 +Y AY +LPF W + IGL+ + F + LI L + Sbjct: 16 YYCAYGVFLPFFPAWLKSQQYGEEMIGLVIGSAYIFRFAGGLFFSSLIKKANHIIIALRL 75 Query: 77 STGITPWVVIAYRFAGHHFWLYIVISVVLAVFSSATLPVIDAVTVRHAKHNSLSYGAIRV 136 + ++ A H+FWL + + A +SA +P+ D++ + L YG +R+ Sbjct: 76 LALASAIIMAAMSLVAHNFWLLFIAIGLYASVNSAGMPIGDSLASTWQRQIGLDYGKVRL 135 Query: 137 VGSISFGLAVAVFGQMYEHFGISTVFAAYILTM-----TLVCLLTFSIPGDRKGRPKPEM 191 +GS +F L V VFG M G + +ILT T++ LL +IP PK E+ Sbjct: 136 IGSAAFILGVVVFGGMIGWVGEQNI--VWILTALLSFYTIIQLLKPTIP------PKDEI 187 Query: 192 AGKGAPRSGMTAGMITLLKEPKFVWFLIPVFLAAIGPQMNNAFYSVYISHFG---GEASA 248 G I LLK P + +I V L Q ++A Y VY + + G + + Sbjct: 188 PEDSVQND---VGFIALLKNPMTLRVMIAVGLI----QGSHAAYYVYSTIYWTSIGISVS 240 Query: 249 KIGLLYTAASLTEIPVFLFSGYIIRKFGYVKILTAVSLAGALRWYVLSLEPSFEILMANQ 308 + LL+ L EI +F FS + + +L +LA RW V+ F ++ Q Sbjct: 241 QTSLLWGIGVLAEIVLFFFSRRLFQNISISVLLYISALACVGRWAVIGYIEDFWLIFLLQ 300 Query: 309 MLSGLTYALFLSAGVNY-AYDNSSDRAKTT------AHSLFVVVYTNVAGIV 353 ++ LTYA+ A V Y S AK ++ + + ++T +AG++ Sbjct: 301 LMHSLTYAVCHYAIVRYITTQPQSHIAKLQGLYNGLSNGVLIAIFTAIAGMI 352 >TM1032 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 381 Score = 78.6 bits (192), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 92/368 (25%), Positives = 151/368 (41%), Gaps = 32/368 (8%) Query: 4 SYRKYSLHVYNYFFYIAYASYLPFISYWFAEDGLSTQQIGLIFSIGPLVGFLVQPLWGML 63 S+ +Y L +F Y A Y +S + A + LS QIG++ +I P+ L + Sbjct: 11 SFVRYRLMSIEFFVYGTMAVY-SLLSQFLANEELSKSQIGILMAILPITSLYANHLNFEI 69 Query: 64 IDYYGMAKWL--LLISTGITPWVVIAYRFAGHHFWLYIVISVVLAVFSSATLPVIDAVTV 121 G WL +++ GI W + F F+L ++ V A F SA P+ ++V V Sbjct: 70 ASTIGRVNWLKRVILFAGILFWGL----FLFDEFYLKLLFMAVFAFFFSAVTPLSESVIV 125 Query: 122 RHAKHNSLSYGAIRVVGSISFGLAVAVFGQMYEHFGISTVFAAYILTMTLVCLLTFSIPG 181 ++YG IR+ G+ F + + G T+F T T + LTF I Sbjct: 126 DITYQMKMNYGRIRLFGTFGFSFTALLMSGLI-RLGFVTIFC----TFTTLMFLTFLILN 180 Query: 182 DRKGRPKPEMAGKGAPRSGMTAGMITLLKEPKFVWFLIPVFLAAIGPQMNNAFYSVYISH 241 K E + + + P F W L+PV + I N F V++ Sbjct: 181 PMKEVELGEEEKRKSKKP-----------LPTFFWLLLPVVIFGIAA---NNFNFVFLPV 226 Query: 242 FGGEASAKIGLLYTAASL---TEIPVFLFSGYIIRKFGYVKILTAVSLAGALRWYVLSLE 298 E + + L A SL TE P L++ I++K G +L + LR ++++ Sbjct: 227 LMKERNYDVSLASIALSLMAITETPFLLWADEIVKKLGVGFMLASGVFVVGLRNLLVTMT 286 Query: 299 PSFEILMANQMLSGLTYALFLSAGVNYAYDNSSDRAKTTAHSLFVVVYTNVAGIVASNVG 358 S L+ Q+L G TY + + + S R + + V + + S+VG Sbjct: 287 GSPAALLMVQLLQGWTYIVIYYSMMFLIRTFGSARIRAQKY---FWVAMGIGPFIGSSVG 343 Query: 359 GWVVERGG 366 G + E G Sbjct: 344 GVLAENLG 351 >STM1032 [R] COG5511 Bacteriophage capsid protein Length = 437 Score = 78.6 bits (192), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 18/368 (4%), Positives = 50/368 (13%), Gaps = 32/368 (8%) Query: 4 SYRKYSLHVYNYFFYIAYASYLPFISYWFAEDGLSTQQIGLIFSIGPLVGFLVQPLWGML 63 + A A + + L+ + Sbjct: 11 IIVEPQPLTVAGTLNNALAEQ-IRARWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVF 69 Query: 64 IDYYGMAKWL--LLISTGITPWVVIAYRFAGHHFWLYIVISVVLAVFSSATLPVIDAVTV 121 + + + + L + Sbjct: 70 SQMVAGKMPGLEPVAGVPFWLEAM----EPDYVPMEQTDSTNNLIQGIYFNDWQRPKSYI 125 Query: 122 RHAKHNSLSYGAIRVVGSISFGLAVAVFGQMYEHFGISTVFAAYILTMTLVCLLTFSIPG 181 + + + + F + + ++ LL Sbjct: 126 VCKSWPGFATAMVATKLIDAENMLHLKFTRRL-NQARGVTLL----APVIIRLLDLKEYE 180 Query: 182 DRKGRPKPEMAGKGAPRSGMTAGMITLLKEPKFVWFLIPVFLAAIGPQMNNAFYSVYISH 241 D + A + A + Sbjct: 181 DSERLAARISAAFAMFIR-----------RSDAMVQDGDAPDYADKD---RDLDIEPGTI 226 Query: 242 FGGEASAKIGLLYTAASL---TEIPVFLFSGYIIRKFGYVKILTAVSLAGALRWYVLSLE 298 + + E + A + G L Sbjct: 227 LKDLLPGEDIGTIKSDRPNANLESFRMGQLRAVAAGVRGSFSSIARNYDGTYSAQRQELV 286 Query: 299 PSFEILMANQMLSGLTYALFLSAGVNYAYDNSSDRAKTTAHSLFVVVYTNVAGIVASNVG 358 + E Q + + + T + A + Sbjct: 287 EAQEGYAILQDNFIAAVSRPVYRRWLATAITAGVIDVPTDT---DMATLFNAVYSGPVMP 343 Query: 359 GWVVERGG 366 + Sbjct: 344 WIDPLKEA 351 >VC0302 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 396 Score = 78.2 bits (191), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 90/377 (23%), Positives = 160/377 (42%), Gaps = 32/377 (8%) Query: 12 VYNYF--FYIAYASYLPFISYWFAEDGLSTQQIGLIFSIG----PLVGFLVQPLWGMLID 65 + YF F+ AY YLPF S WF + G+S IG++ IG + ++ P + Sbjct: 10 ISQYFIGFFFAYGVYLPFWSLWFEDQGVSATDIGVLVGIGFATRCVANLVLTPRLHRVEQ 69 Query: 66 YYGMAKWLLLISTGITPWVVIAYRF-AGHHFWLYIVISVVLAVFSSATLPVIDAVTVRHA 124 +WL + + + + F G FWL + +V+ + +P+ DA+ +A Sbjct: 70 LMPALRWLSFAAM-----LFVGFHFFTGGSFWLMALATVLFNLCCGPVVPISDAMANYYA 124 Query: 125 KHNSLSYGAIRVVGSISFGLAVAVFGQMYEHFGISTVFAAYILTMTLVCLLTFSIPGDRK 184 K L YG R+ GSI+F V G + +G + ++ + + LL+ R+ Sbjct: 125 KLKMLDYGRTRLWGSIAFIAGSTVVGFLVAKWGSLMILYTALVGVAVSWLLSL-----RQ 179 Query: 185 GRPKPEMAGKGAPRSGMTAGMITLLKEPKFVWFLIPVFLAAIGPQMNNAFYSVYISHFGG 244 P P + + + LL E V FL+ V L +F S+Y G Sbjct: 180 ITPMPVTEHEA---HAVRPKLKELLTEWPVVKFLLLVALIQGSHAAYYSFGSIYWKQ-AG 235 Query: 245 EASAKIGLLYTAASLTEIPVFLFS-----GYIIRKFGYVKILTAVSLAGALRWYVLSLEP 299 + IG L++ + E+ VF FS G+ +R +V L ++ RW + + Sbjct: 236 HSEDIIGYLWSLGVVAEVLVFAFSKRWFAGWSLRTLFFVASLGVIA-----RWGLTASTT 290 Query: 300 SFEILMANQMLSGLTYALFLSAGVNYAYDNSSDRAKTTAHSLFVVVYTNVAGIVASNVGG 359 + L+A Q+L G+T+A+ A + Y ++ +L+ + + + G Sbjct: 291 TILALVAIQLLHGVTFAIAHIAAIQY-IQHAPQNKMVALQALYNAIPLGAVIAAVTTLSG 349 Query: 360 WVVERGGYSLLFQGAAV 376 W E G ++ + AA+ Sbjct: 350 WGYEHWGAAVFWAMAAM 366 >ECs0396 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 417 Score = 59.3 bits (142), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 89/382 (23%), Positives = 153/382 (40%), Gaps = 33/382 (8%) Query: 23 SYLPFISYWFAE-DGLSTQQIGLIFSIGPLVGFLVQPLWGMLIDYYGMAKWLLLISTGI- 80 +Y PF W + + +S G+IF+ L L QPL+G+L D G+ K+LL I TG+ Sbjct: 25 AYFPFFPIWLHDINHISKSDTGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGML 84 Query: 81 ---TPWVVIAYRFAGHHFWLY--IVISVVLAVFSSATLPVIDAVTVRHAKHNSLSYGAIR 135 P+ + + + L IV + L +A P ++A + ++ ++ +G R Sbjct: 85 VMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRAR 144 Query: 136 VVGSISFGLAVAVFGQMYEHFGISTVFAAYI---LTMTLVCLLTFSIPGDRKGRPKPEMA 192 + G + + L ++ G M F I+ F ++ + L LL F+ + P Sbjct: 145 MFGCVGWALCASIVGIM---FTINNQFVFWLGSGCALILAILLFFA----KTDAPSSATV 197 Query: 193 GK--GAPRSGMTAGM-ITLLKEPKFVWFL------IPVFLAAIGPQMNNAFYSVYISHFG 243 GA S + + + L ++PK +WFL + Q N F S + + G Sbjct: 198 ANAVGANHSAFSLKLALELFRQPK-LWFLSLYVIGVSCTYDVFDQQFANFFTSFFAT--G 254 Query: 244 GEASAKIGLLYTAASLTEIPVFLFSGYIIRKFGYVKILTAVSLAGALRWYVLSLEPSFEI 303 + + G + T L + F+ II + G L ++R S S Sbjct: 255 EQGTRVFGYVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALE 314 Query: 304 LMANQMLSGLTYALFLSAGVNYAYDNSSDRAKTTAHSLFVVVYTNVAGIVASNVGGWVVE 363 ++ + L L Y R T + + + +A I S + G + E Sbjct: 315 VVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYE 374 Query: 364 RGGYSLLFQGAAVMSLLGAAGF 385 G FQGA ++ L A GF Sbjct: 375 SIG----FQGAYLVLGLVALGF 392 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.141 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,926,066 Number of extensions: 1203966 Number of successful extensions: 4185 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 4178 Number of HSP's successfully gapped: 14 Length of query: 400 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 291 Effective length of database: 25,807,025 Effective search space: 7509844275 Effective search space used: 7509844275 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)