BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0096.p (286 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SSO2112 [R] COG2159 Predicted metal-dependent hydrolase of the T... 117 3e-26 AGc2623 [R] COG2159 Predicted metal-dependent hydrolase of the T... 116 4e-26 Rv2303c [R] COG2159 Predicted metal-dependent hydrolase of the T... 95 1e-19 MT2360 [R] COG2159 Predicted metal-dependent hydrolase of the TI... 95 1e-19 Rv3510c [R] COG2159 Predicted metal-dependent hydrolase of the T... 93 5e-19 >SSO2112 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 269 Score = 117 bits (292), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 78/259 (30%), Positives = 126/259 (48%), Gaps = 24/259 (9%) Query: 32 YKQSPYSIESVKIEMDY---MNVDKSVLLPEDLTTQSGGCIVTNEEIAKLVSLEPDRFIG 88 Y + ++ +K +DY + + V+LP D TT G I ++++L+ DR + Sbjct: 27 YFNAKVEVKGLKETLDYYESFGIKRFVVLPIDSTTFLGRRIPN-----QVINLD-DRIVK 80 Query: 89 FASVDPYRKDAAAVLDYAFKELGLKGLKLHPSKQRFYPYDKLLKPIYEKCLEYDKPIMFH 148 F SVDP + +A L + KE G+KLHP Q F+P D+ IYE + ++FH Sbjct: 81 FISVDPSKPNADEELKRSIKEFEPVGVKLHPQLQGFHPLDERALKIYEIIDNHGLIVVFH 140 Query: 149 AGMTW-----QPDAPAKYSHPLHFEEVAMAYPGLRMCLAHFGWPWIHETVMLLLKYPNVY 203 G + + Y P++F+E+A+ + +++ LAHFGWPW E + + L PNVY Sbjct: 141 TGTSGIGAGVKSSIRLDYGRPIYFDEIAVRFQNMKIILAHFGWPWTEEAIAIALHKPNVY 200 Query: 204 TDTSLLHMDNAKDFYEQVFTRNMGPLWIERNLKYQVMFGTNSPRFRAKRLLPALKGLNFR 263 D S A + QV N + L +++FG++ P R +R L K +N Sbjct: 201 LDLS----GWAPRYIPQVIWSNA------KRLSDKLLFGSDFPLIRPERWLEEFKSVNLS 250 Query: 264 PSTLENILSGNAMRFIGGS 282 + IL NA + + S Sbjct: 251 QDIKDKILKHNAEKLVKKS 269 >AGc2623 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 291 Score = 116 bits (290), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 85/295 (28%), Positives = 139/295 (47%), Gaps = 28/295 (9%) Query: 1 MIIDVHTHPIFYKDICEDPEQLKFRKEQFGIYK--QSPYS----IESVKIEMDYMNVDK- 53 + IDVHTH ++ C P + + Q G+ K ++P + +V+ Y K Sbjct: 7 IAIDVHTHA---EEPCCGPRDDGYDEFQAGMAKYFKNPAGHKGMLPTVQETAAYYRERKI 63 Query: 54 -SVLLPEDLTTQSGGCIVTNEEIAKLVSLEPDRFIGFASVDPYRKDAAAV-LDYAFKELG 111 V+ P D ++G NEE+AK+ + D I FAS+DP + A A +E G Sbjct: 64 GCVIFPVDAERETGFRRYENEEVAKIAAENSDIMIPFASIDPAKGKAGAREARRLVREFG 123 Query: 112 LKGLKLHPSKQRFYPYDKLLKPIYEKCLEYDKPIMFHAGMTWQPDA-------PAKYSHP 164 +KG K HP+ Q FYP D++ P+YE E +FH G T K+S+P Sbjct: 124 VKGFKFHPTMQGFYPNDRMAYPLYEAIAEEGAITLFHTGQTGVGAGLRGGMAMRLKFSNP 183 Query: 165 LHFEEVAMAYPGLRMCLAHFGWPWIHETVMLLLKYPNVYTDTSLLHMDNAKDFYEQVFTR 224 +H ++VA+ +P + + LAH +PW E + + PNVY D S + ++ + + Sbjct: 184 IHLDDVAVDFPDMPIILAHPSFPWQEEALSVATHKPNVYIDMSGW----SPKYFPPILVQ 239 Query: 225 NMGPLWIERNLKYQVMFGTNSPRFRAKRLLPALKGLNFRPSTLENILSGNAMRFI 279 L LK++++FG++ P +R L ++ + IL NA R + Sbjct: 240 YANSL-----LKHKMLFGSDWPAMTPERWLNDFANISIKDEVRPLILKENARRLL 289 >Rv2303c [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 307 Score = 95.1 bits (235), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 53/179 (29%), Positives = 85/179 (47%), Gaps = 2/179 (1%) Query: 27 EQFGIYKQSPYSIESVKIE-MDYMNVDKSVLLPEDLTTQSGGCIVTNEEIAKLVSLEPDR 85 EQFG Y +S + ++ +D + +S++ D + G V N +A + + PDR Sbjct: 50 EQFGAYAESLRRTDDQILDSLDDAGITRSLITGFDERSTCGVTFVHNASVAAVAARYPDR 109 Query: 86 FIGFASVDPYRKDAAA-VLDYAFKELGLKGLKLHPSKQRFYPYDKLLKPIYEKCLEYDKP 144 F+ FA D D+A + E G +GL L P D P Y KC+E P Sbjct: 110 FLPFAGADILAGDSAVDEFERWVVEHGFRGLSLRPFMIGRPASDPAYFPCYAKCVELGVP 169 Query: 145 IMFHAGMTWQPDAPAKYSHPLHFEEVAMAYPGLRMCLAHFGWPWIHETVMLLLKYPNVY 203 + H W + HP H ++VA +P L + ++H G+PW+ + ++ K+PNVY Sbjct: 170 VSIHTSADWTRTRLSDLGHPRHIDDVACRFPELTILMSHGGYPWVLQACLIAWKHPNVY 228 >MT2360 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 307 Score = 95.1 bits (235), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 53/179 (29%), Positives = 85/179 (47%), Gaps = 2/179 (1%) Query: 27 EQFGIYKQSPYSIESVKIE-MDYMNVDKSVLLPEDLTTQSGGCIVTNEEIAKLVSLEPDR 85 EQFG Y +S + ++ +D + +S++ D + G V N +A + + PDR Sbjct: 50 EQFGAYAESLRRTDDQILDSLDDAGITRSLITGFDERSTCGVTFVHNASVAAVAARYPDR 109 Query: 86 FIGFASVDPYRKDAAA-VLDYAFKELGLKGLKLHPSKQRFYPYDKLLKPIYEKCLEYDKP 144 F+ FA D D+A + E G +GL L P D P Y KC+E P Sbjct: 110 FLPFAGADILAGDSAVDEFERWVVEHGFRGLSLRPFMIGRPASDPAYFPCYAKCVELGVP 169 Query: 145 IMFHAGMTWQPDAPAKYSHPLHFEEVAMAYPGLRMCLAHFGWPWIHETVMLLLKYPNVY 203 + H W + HP H ++VA +P L + ++H G+PW+ + ++ K+PNVY Sbjct: 170 VSIHTSADWTRTRLSDLGHPRHIDDVACRFPELTILMSHGGYPWVLQACLIAWKHPNVY 228 >Rv3510c [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 278 Score = 93.2 bits (230), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 69/256 (26%), Positives = 111/256 (43%), Gaps = 12/256 (4%) Query: 23 KFRKEQFGIYKQSPYSIESVKIEMDYMNVDKSVLLPEDLTTQSGGCIVTNEEIAKLVSLE 82 R+ G ++ IE+ MD V +L +G +++N+ +A+ V L Sbjct: 22 SLRRWTGGSIPETDIPIEATVSSMDAGGVTLGLL--SAWRGPNGQDLISNDAVAEWVRLY 79 Query: 83 PDRFIGFASVDPYRKDAAAVLDYAFKELGLKGLKLHPSKQRFYPYDKLLKPIYEKCLEYD 142 P+RF G A+VD R AA G GL++ P P D+ P++ +C++ Sbjct: 80 PNRFAGLAAVDLDRPMAAVRELRRRVGEGFVGLRVVPWLWGAPPTDRRYYPLFAECVQSA 139 Query: 143 KPIMFHAGMTWQPDAPAKYSHPL-HFEEVAMAYPGLRMCLAHFGWPWIHETVMLLLKYPN 201 P G T P P++ P+ + ++VA+ +P L + H G+PW E V + K+ N Sbjct: 140 VPFCTQVGHT-GPLRPSETGRPIPYIDQVALDFPELVIVCGHVGYPWTEEMVAVARKHEN 198 Query: 202 VYTDTSLLHMDNAKDFYEQVFTRNMGPLWIERNLKYQVMFGTNSPRFRAKRLLPALKGLN 261 VY DTS + + + G + +V+FGTN P L L L Sbjct: 199 VYIDTSAYTIKRLPGKLVRFMKTDTG--------QRKVLFGTNYPMIAHTHALTGLDELG 250 Query: 262 FRPSTLENILSGNAMR 277 + L GNA+R Sbjct: 251 LSDEARRDFLHGNAVR 266 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.140 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,231,623 Number of extensions: 955397 Number of successful extensions: 1326 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 1307 Number of HSP's successfully gapped: 10 Length of query: 286 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 181 Effective length of database: 26,268,361 Effective search space: 4754573341 Effective search space used: 4754573341 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)