BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0095.p (259 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yhfQ [P] COG4594 ABC-type Fe3+-citrate transport system, peri... 190 2e-48 Z4734m [G] COG0524 Sugar kinases, ribokinase family 183 2e-46 BS_yurL [G] COG0524 Sugar kinases, ribokinase family 125 5e-29 STM3600 [G] COG0524 Sugar kinases, ribokinase family 101 9e-22 CAC3498 [G] COG0524 Sugar kinases, ribokinase family 95 1e-19 >BS_yhfQ [P] COG4594 ABC-type Fe3+-citrate transport system, periplasmic component Length = 261 Score = 190 bits (482), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 96/252 (38%), Positives = 158/252 (62%), Gaps = 1/252 (0%) Query: 3 VAGVGFNCMDIYDNLQRYYPTGNSVDFIIHMSRFGVQSSMVSAVGADKHGELMIEVLRRE 62 +A +G NC+DIY L + + GN+V+ ++ +R+G+Q ++ VG D +G + + L R Sbjct: 4 LATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCITWVGDDDYGTKLKQDLARM 63 Query: 63 NVDVSHLHVEEGDTAIFKMDLNGNDRVHKEKLAGVMENFALTEDDIAFVLEHGMIHTNLS 122 VD+SH+H + G TA +++L+ NDRV + GVM +FAL+E+D A++ ++ ++H + Sbjct: 64 GVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADFALSEEDYAWLAQYDIVHAAIW 123 Query: 123 GNIIEWLPRFRASGVLVVFDFTTKVNS-IPEPILPDVDYAFFSYDQDDTWILEYMKWAQA 181 G+ + P+ A+G L FDF+ K +S + + ++P +D+AF S Q+D + MK A Sbjct: 124 GHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHLDFAFASAPQEDETLRLKMKAIVA 183 Query: 182 LGPRVVVVTLGEKGSIAYDGQSFFREGILKVPVVNTVGAGDSFCAGFMYGVIQGRSIPDS 241 G V+VTLGE GSIA+DG F+R+ V V++T+GAGDSF AGF+ G G ++P + Sbjct: 184 RGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVIDTMGAGDSFIAGFLCGWSAGMTLPQA 243 Query: 242 LRIGAETAAAVV 253 + G AA + Sbjct: 244 IAQGTACAAKTI 255 >Z4734m [G] COG0524 Sugar kinases, ribokinase family Length = 262 Score = 183 bits (465), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 94/252 (37%), Positives = 157/252 (62%), Gaps = 1/252 (0%) Query: 3 VAGVGFNCMDIYDNLQRYYPTGNSVDFIIHMSRFGVQSSMVSAVGADKHGELMIEVLRRE 62 +A +G NC+DIY L + + GN+V+ ++ +R+G++ ++ VG D +G + + L Sbjct: 5 LATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIKPGCITWVGDDDYGTKLKQDLASM 64 Query: 63 NVDVSHLHVEEGDTAIFKMDLNGNDRVHKEKLAGVMENFALTEDDIAFVLEHGMIHTNLS 122 VD+SH+H + G TA +++L+ NDRV + GVM +FAL+E+D A + ++ ++H + Sbjct: 65 GVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADFALSEEDYAXLAQYDIVHAAIW 124 Query: 123 GNIIEWLPRFRASGVLVVFDFTTKVNS-IPEPILPDVDYAFFSYDQDDTWILEYMKWAQA 181 G+ + P+ A+G L FD + K +S + + ++P +D+AF S Q+D + MK A Sbjct: 125 GHAEDAFPQLHAAGKLTAFDVSDKWDSPLWQTLVPHLDFAFASAPQEDEALRLKMKAIVA 184 Query: 182 LGPRVVVVTLGEKGSIAYDGQSFFREGILKVPVVNTVGAGDSFCAGFMYGVIQGRSIPDS 241 G VV+VTLGE GSIA+DG F+R+ +V V++T+GAGDSF AGF+ G G ++P + Sbjct: 185 RGAGVVIVTLGENGSIAWDGVQFWRQAPEQVTVIDTMGAGDSFIAGFLCGWSAGMTLPQA 244 Query: 242 LRIGAETAAAVV 253 + G AA + Sbjct: 245 MAQGTACAAQTI 256 >BS_yurL [G] COG0524 Sugar kinases, ribokinase family Length = 284 Score = 125 bits (315), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 81/263 (30%), Positives = 138/263 (52%), Gaps = 9/263 (3%) Query: 1 MRVAGVGFNCMDIYDNLQRYYPTGNSVDFIIHMSRFGVQSSMVSAVGADKHGELMIEVLR 60 M++ VG N +D Y + + +YP GN+++ + R G +SS + VG D+ ++ VL+ Sbjct: 1 MKLIAVGDNVVDYYQDQETFYPGGNALNVAVLAKRLGHESSYIGIVGNDEAAAHLLNVLK 60 Query: 61 RENVDVSHLHVEEGDT--AIFKMDLNGNDRVHKEKLAGVMEN---FALTEDDIAFVLEHG 115 E V+ ++ G+ AI +D G DR+ G +++ A E D++F+ H Sbjct: 61 LEQVNADYIRQAHGENGMAIVTLDEQG-DRIFVRSNKGGIQSRLRLAFQEKDVSFISGHD 119 Query: 116 MIHTNLSGNIIEWLPRFRASGVLVVFDFTT-KVNSIPEPILPDVDYAFFS-YDQDDTWIL 173 ++HT++ + LP+ V V FDF+T + + + P V YAFFS D ++ Sbjct: 120 LLHTSVYSRLENDLPQL-CGLVPVSFDFSTNREDDYLRRVCPYVTYAFFSGSDLSESECG 178 Query: 174 EYMKWAQALGPRVVVVTLGEKGSIAYDGQSFFREGILKVPVVNTVGAGDSFCAGFMYGVI 233 E K A G ++V +T G +G+I G + + I++ +++T+GAGDSF AGF+ Sbjct: 179 ELAKTAHGYGAKMVCMTRGGQGAILSAGDRVYHQPIVEADIIDTLGAGDSFIAGFLTAFC 238 Query: 234 QGRSIPDSLRIGAETAAAVVAKF 256 + I +LR AETAA + Sbjct: 239 VKQDITYALRQAAETAAKTCGVY 261 >STM3600 [G] COG0524 Sugar kinases, ribokinase family Length = 281 Score = 101 bits (252), Expect = 9e-22, Method: Compositional matrix adjust. Identities = 71/269 (26%), Positives = 139/269 (51%), Gaps = 14/269 (5%) Query: 1 MRVAGVGFNCMDIYDNLQRYYPTGNSVDFIIHMSRFGVQSSMVSAVGADKHGELMIEVLR 60 + V G+G N +D Y + YP GN+++F ++ + S+ + A G D + + +VL Sbjct: 3 ISVLGIGDNVVDKYLHSGIMYPGGNALNFAVYAKLADIPSAFMGAFGNDDAAQHVQDVLH 62 Query: 61 RENVDVSHLHVEEGDTAIFKMDLNGNDR--VHKEKLAGVMEN-FALTEDDIAFVLEHGMI 117 + +D+SH G+ + L+ DR V K + E+ F+L++DD+ ++ + ++ Sbjct: 63 QLQIDISHSRHYTGENGYACIRLSHGDRQFVASNKNGVLREHPFSLSDDDLRYISQFTLV 122 Query: 118 HTNLSGNIIEWLPRFRASGVLVVFDFTTK-VNSIPEPILPDVDYAFFSYD--QDDTWILE 174 H++++G++ L + + VL+ FDF+ + + E + P VDY F S D ++ Sbjct: 123 HSSINGHLESELEKIKQQTVLLSFDFSGRGTDDYFEKVCPWVDYGFISCSGLSPDEIKVK 182 Query: 175 YMKWAQALGPRVVVVTLGEKGSIAYDGQSFFREGILKVPVVNTVGAGDSFCAGFMYGVIQ 234 K + G R ++ T G + + G + + V+T+GAGD+F GF+ ++Q Sbjct: 183 LNKLYR-YGCRHIIATCGHEKVYYFSGADYLEWQPAYIEPVDTLGAGDAFLTGFLLSILQ 241 Query: 235 -GRSIPD------SLRIGAETAAAVVAKF 256 G + PD ++R G ++AA V++ + Sbjct: 242 SGMAEPDKESVLRAMRQGGKSAAQVLSHY 270 >CAC3498 [G] COG0524 Sugar kinases, ribokinase family Length = 295 Score = 94.7 bits (234), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 68/268 (25%), Positives = 129/268 (48%), Gaps = 13/268 (4%) Query: 2 RVAGVGFNCMDIYDNLQRYYPTGNSVDFIIHMSRFGVQSSMVSAVGADKHGELMIEVLRR 61 ++ +G N D Y + + YP G ++ ++ G +++ + G D+ E + L+ Sbjct: 5 KIVAIGDNVCDKYLSRGKMYPGGQCINTCVYGKMNGAETAYIGKYGNDEVAECVQNTLKE 64 Query: 62 ENVDVSHLHVEEGDTAIFKMDLNGNDRVHKEKLAGVM---ENFALTEDDIAFVLEHGMIH 118 +D SH EG+ + L DRV G + ++ E+D ++ + +I+ Sbjct: 65 IGIDDSHCRHYEGENGFALVTLKNADRVFLGSNKGGIAKEHSYNFDENDFEYIKKFDLIY 124 Query: 119 TNLSGNIIEWLPRFRASGVLVVFDFTTK-VNSIPEPILPDVDYAFFSYDQDDTWILEY-M 176 TNL+ I + L A+GV + +DF+ + + + P +D A S E M Sbjct: 125 TNLNSYIEDNLKELHATGVPIAYDFSMRWTDEYLLKVCPYIDVAILSCAHLTAEEREKEM 184 Query: 177 KWAQALGPRVVVVTLGEKGS-IAYDGQSFFREGILKVPVVNTVGAGDSFCAGFMYGVIQ- 234 K Q G ++V+ T+GE GS + Y+G + I+ +++T+GAGDS+ + F+ ++Q Sbjct: 185 KKVQNYGVKIVLGTIGEDGSYVLYNGDFLYTSAIMAANIIDTMGAGDSYFSTFLCSLLQT 244 Query: 235 ---GRSI---PDSLRIGAETAAAVVAKF 256 G+ + +S++I E+A A F Sbjct: 245 SETGKLVEGTKESMKIRLESAMKSGAIF 272 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.139 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,243,866 Number of extensions: 839026 Number of successful extensions: 2111 Number of sequences better than 1.0e-05: 43 Number of HSP's gapped: 2114 Number of HSP's successfully gapped: 43 Length of query: 259 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 155 Effective length of database: 26,383,695 Effective search space: 4089472725 Effective search space used: 4089472725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)