BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0080.p (496 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1856_2 [G] COG1263 Phosphotransferase system IIC components, g... 256 6e-68 BS_sacX_2 [G] COG1263 Phosphotransferase system IIC components, ... 251 1e-66 BS_sacP_2 [G] COG1263 Phosphotransferase system IIC components, ... 207 3e-53 CAC0423_2 [G] COG1263 Phosphotransferase system IIC components, ... 206 6e-53 VCA0653_2 [G] COG1263 Phosphotransferase system IIC components, ... 173 7e-43 >BH1856_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 362 Score = 256 bits (654), Expect = 6e-68, Method: Compositional matrix adjust. Identities = 148/362 (40%), Positives = 202/362 (55%), Gaps = 10/362 (2%) Query: 127 LKQIADAVSFFSDIVVPMIPXXXXXXXXXXXXSMMEVFGWAPRDSAAFRTLSLLTGSAFQ 186 + IA S+I VP+IP MM F W D A L + + +AF Sbjct: 3 MNPIARFAKTLSNIFVPIIPAIVASGLLMGLLGMMRTFEWVAPDHALIVLLDMFSSAAFI 62 Query: 187 MLAVMFGYHTAKRFGGTPPLGAVIGLVMTHPGLLHVTGFGRAPVSSADVMIAPQF----- 241 +L ++ G AK FGG P LGAV+G +MTHP LL+ G G A D + F Sbjct: 63 ILPILIGVSAAKEFGGNPYLGAVMGGIMTHPALLNPWGLGNA---EPDYLTFLGFDIALI 119 Query: 242 GYQGAVIPTILAVLVMTLIEKGLRRMLPASASPMLIPFXXXXXXXXXXXXXXXPLTSELS 301 GYQG VIP +LAV M+ IEKGLR+++P + ++ PF P+ + + Sbjct: 120 GYQGTVIPVLLAVYAMSKIEKGLRKVVPNAIDLLVTPFVTIMLTGFISLMVIGPIGNFIG 179 Query: 302 ASLGSMLELVFRSGGTVFGLLLGGIYSSLVVSGLHHGIQAIEIGLITNPDISVNFLLPIW 361 + ++L V+ G V GLL GGIYS++V++G+HH AIE GLI + I +N+LLPIW Sbjct: 180 DGITTILNFVYDHVGFVAGLLFGGIYSAIVITGVHHSFHAIEAGLIAS--IGINYLLPIW 237 Query: 362 SMANIAQGAAGLAVYARTRDPALRKIALPASITAFLGITEPVTFGVNLKLGRPFLXXXXX 421 SMAN+AQG AGLAV+ +T+ +++ALP++ +AFLGITEPV FGVNLK RPF+ Sbjct: 238 SMANVAQGGAGLAVFFKTKRAKTKEVALPSAFSAFLGITEPVIFGVNLKYRRPFVGAMIG 297 Query: 422 XXXXXXYVAFHQVAADSFGLTGIPMFAFTVQLGQLNVIHYXXXXXXXXXXXXXVTWVLGV 481 YV F V+A ++GLTGIPM A LG +N++HY TW+LG Sbjct: 298 GALGGAYVVFMNVSAVAYGLTGIPMLAIVSPLGTINLLHYVIGFFIALVTAFVATWLLGF 357 Query: 482 DE 483 E Sbjct: 358 KE 359 >BS_sacX_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 365 Score = 251 bits (642), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 145/348 (41%), Positives = 198/348 (56%), Gaps = 2/348 (0%) Query: 138 SDIVVPMIPXXXXXXXXXXXXSMMEVFGWAPRDSAAFRTLSLLTGSAFQMLAVMFGYHTA 197 SDI VP+IP M++VF W S + L L++ +AF +L ++ G+ A Sbjct: 15 SDIFVPIIPAITASGLLMGLIGMIKVFHWFAAGSPWIKMLDLVSSTAFILLPILVGFSAA 74 Query: 198 KRFGGTPPLGAVIGLVMTHPGLLHVTGFGRAPVSSADV--MIAPQFGYQGAVIPTILAVL 255 ++FG P LGAVI ++THP LL + G SS D+ + P GYQG++IP +L+V Sbjct: 75 RQFGSNPYLGAVIAGLLTHPDLLDPSMLGSKTPSSLDIWGLHIPMMGYQGSMIPILLSVF 134 Query: 256 VMTLIEKGLRRMLPASASPMLIPFXXXXXXXXXXXXXXXPLTSELSASLGSMLELVFRSG 315 VM+ IEK L+ ++P S ++IPF P S + + + ++ Sbjct: 135 VMSKIEKLLKSIVPKSLDVVIIPFITVMVTGCLALIVMNPAASIIGQIMTQSIVYIYDHA 194 Query: 316 GTVFGLLLGGIYSSLVVSGLHHGIQAIEIGLITNPDISVNFLLPIWSMANIAQGAAGLAV 375 G G L GGIYS++V+SGLHH AIE L+ NP + VNFL+PIWSMAN+AQG AGLAV Sbjct: 195 GIAAGALFGGIYSTIVLSGLHHSFYAIEATLLANPHVGVNFLVPIWSMANVAQGGAGLAV 254 Query: 376 YARTRDPALRKIALPASITAFLGITEPVTFGVNLKLGRPFLXXXXXXXXXXXYVAFHQVA 435 + +T+ +L+KIALPAS+TAFLGI EP+ FGVNLKL RPF+ YV QV Sbjct: 255 FLKTKQSSLKKIALPASLTAFLGIVEPIVFGVNLKLIRPFIGAAIGGAIGGAYVVAVQVV 314 Query: 436 ADSFGLTGIPMFAFTVQLGQLNVIHYXXXXXXXXXXXXXVTWVLGVDE 483 A+S+GLTGIPM + + G N +HY T LG E Sbjct: 315 ANSYGLTGIPMISIVLPFGAANFVHYMIGFLIAAVSAFIATLFLGFKE 362 >BS_sacP_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 367 Score = 207 bits (527), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 128/353 (36%), Positives = 177/353 (50%), Gaps = 15/353 (4%) Query: 138 SDIVVPMIPXXXXXXXXXXXXSMMEVFGWAPRDSAAFRTLSLLTGSAFQMLAVMFGYHTA 197 S+I VP+IP M+ F W +DSA + L + + +AF L ++ G + Sbjct: 17 SNIFVPIIPAIVASGLLMGLLGMINAFHWMSKDSALLQLLDMFSSAAFIFLPILIGVSAS 76 Query: 198 KRFGGTPPLGAVIGLVMTHPGLLHVTGFGRAPVSSA---DVMIAPQFGYQGAVIPTILAV 254 K FG P LGAVIG +M HP LL+ G + + D++ F G P Sbjct: 77 KEFGSNPYLGAVIGGIMIHPNLLNPWGLAEEQLITCIFSDLI--SLFSATGNCYPC---- 130 Query: 255 LVMTLIEKGLRRMLPASASP----MLIPFXXXXXXXXXXXXXXXPLTSELSASLGSMLEL 310 + ++ R M S S ++ PF PL L + + L Sbjct: 131 PACGVCDEQGREMDEKSGSTCGDLLVTPFVTVIVTGFVAFIAIGPLGRALGSGITVALTY 190 Query: 311 VFRSGGTVFGLLLGGIYSSLVVSGLHHGIQAIEIGLITNPDISVNFLLPIWSMANIAQGA 370 V+ G V GL+ GG YS +V++G+HH AIE GLI DI N+LLPIWSMAN+AQG Sbjct: 191 VYDHAGFVAGLIFGGTYSLIVLTGVHHSFHAIEAGLIA--DIGKNYLLPIWSMANVAQGG 248 Query: 371 AGLAVYARTRDPALRKIALPASITAFLGITEPVTFGVNLKLGRPFLXXXXXXXXXXXYVA 430 AGLAV+ + ++IALPA+ +AFLGITEPV FGVNL+ +PF+ YV Sbjct: 249 AGLAVFFMAKKAKTKEIALPAAFSAFLGITEPVIFGVNLRYRKPFIAAMIGGALGGAYVV 308 Query: 431 FHQVAADSFGLTGIPMFAFTVQLGQLNVIHYXXXXXXXXXXXXXVTWVLGVDE 483 F VAA+++GLTGIPM A G N+IHY +V+ ++E Sbjct: 309 FTHVAANAYGLTGIPMIAIAAPFGFSNLIHYLIGMAIAAVSAFIAAFVMKINE 361 >CAC0423_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 361 Score = 206 bits (524), Expect = 6e-53, Method: Compositional matrix adjust. Identities = 123/348 (35%), Positives = 180/348 (51%), Gaps = 5/348 (1%) Query: 138 SDIVVPMIPXXXXXXXXXXXXSMMEVFGWAPRDSAAFRTLSLLTGSAFQMLAVMFGYHTA 197 S+I VP+IP M++ F S + L++ + +AFQ L ++ + A Sbjct: 14 SNIFVPIIPAIVASGLLMGLLGMLDAFHLVNSKSGLYVILNMFSNAAFQFLPMIIAFSAA 73 Query: 198 KRFGGTPPLGAVIGLVMTHPGLLHVTGFGRAPVSSADV--MIAPQFGYQGAVIPTILAVL 255 + F P L A +G +M HP L + G + ++ + GYQG V+P +++V Sbjct: 74 REFKTNPYLAAALGAIMIHPDLQNAWTLGEGIKHTINIFGLNIGMVGYQGTVLPILISVW 133 Query: 256 VMTLIEKGLRRMLPASASPMLIPFXXXXXXXXXXXXXXXPLTSELSASLGSMLELVFRSG 315 VM+ IEKGLR+++P + +L PF P + + L+ ++ + Sbjct: 134 VMSYIEKGLRKIVPEALDILLTPFLTLMITGFFAMVVIGPGGRFVGDEISLGLQTLYNTT 193 Query: 316 GTVFGLLLGGIYSSLVVSGLHHGIQAIEIGLITNPDISVNFLLPIWSMANIAQGAAGLAV 375 G G+L GG+YS +V++G+HH AIE GL+ NP I NFLLPIWSMAN+AQG A LAV Sbjct: 194 GFFSGVLFGGLYSLIVITGIHHSFHAIEAGLLANPAIHKNFLLPIWSMANVAQGGAALAV 253 Query: 376 YARTRDPALRKIALPASITAFLGITEPVTFGVNLKLGRPFLXXXXXXXXXXXYVAFHQVA 435 Y +TRD ++ IA PAS + LGITEP FGVNL+ +PF+ Y+ F +VA Sbjct: 254 YFKTRDKKMKSIAAPASFSCLLGITEPAIFGVNLRYTKPFIAGALGGAIGGGYIVFTKVA 313 Query: 436 ADSFGLTGIPMFAFTVQLGQLNVIHYXXXXXXXXXXXXXVTWVLGVDE 483 + G+TGIP A Q LN Y + VLG+ E Sbjct: 314 MTAVGVTGIPGIAIVKQGSFLN---YIIAMILAFGGAFIIAMVLGIKE 358 >VCA0653_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 386 Score = 173 bits (438), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 100/270 (37%), Positives = 150/270 (55%), Gaps = 11/270 (4%) Query: 185 FQMLAVMFGYHTAKRFGGTPPLGAVIGLVMTHPGLLHVTGFGRAPVS----SADVM--IA 238 F L V+ + +++FGG P LGA +G++M HP LL+ GFG A VS + +++ Sbjct: 74 FVYLPVLLAFSASRKFGGNPFLGAALGMLMVHPDLLNGWGFGSASVSGTVPTWNILGFEI 133 Query: 239 PQFGYQGAVIPTILAVLVMTLIEKGLRRMLPASASPMLIPFXXXXXXXXXXXXXXXPLTS 298 + GYQG+V+P +++ ++ IE GLR+++P+ +L P PLT Sbjct: 134 EKVGYQGSVLPVLVSAYILAKIENGLRKIVPSVVDNLLTPMLAIFITGFLTFTVVGPLTR 193 Query: 299 ELSASLGSMLELVFRSGGTVFGLLLGGIYSSLVVSGLHHGIQAIEIGLITNPDISVN--- 355 ++ LG L ++ S G V G L G IY+ V++G+HH AIE L+ DI Sbjct: 194 DVGFMLGDALNWLYDSAGFVGGALFGFIYAPFVITGMHHSFIAIETQLLA--DIVTTGGT 251 Query: 356 FLLPIWSMANIAQGAAGLAVYARTRDPALRKIALPASITAFLGITEPVTFGVNLKLGRPF 415 F+ PI +M+NIAQGAA LAV T++ L+ +A+P+ +TA LGITEP FGVNLKL PF Sbjct: 252 FIFPIAAMSNIAQGAAALAVGVMTKETKLKGVAIPSGVTALLGITEPAMFGVNLKLRYPF 311 Query: 416 LXXXXXXXXXXXYVAFHQVAADSFGLTGIP 445 + ++ V A + G G+P Sbjct: 312 IAAICGAALASAFITLFNVKAQALGAAGLP 341 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.139 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,984,568 Number of extensions: 1162868 Number of successful extensions: 2348 Number of sequences better than 1.0e-05: 52 Number of HSP's gapped: 2237 Number of HSP's successfully gapped: 52 Length of query: 496 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 385 Effective length of database: 25,576,357 Effective search space: 9846897445 Effective search space used: 9846897445 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 118 (50.1 bits)