BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0075.p (448 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM3718 [M] COG1442 Lipopolysaccharide biosynthesis proteins, LP... 67 6e-11 ZwaaJ [M] COG1442 Lipopolysaccharide biosynthesis proteins, LPS:... 62 2e-09 ECs4502 [M] COG1442 Lipopolysaccharide biosynthesis proteins, LP... 62 2e-09 SP1770 [M] COG1442 Lipopolysaccharide biosynthesis proteins, LPS... 62 2e-09 SP1771_2 [M] COG1442 Lipopolysaccharide biosynthesis proteins, L... 60 7e-09 >STM3718 [M] COG1442 Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases Length = 337 Score = 67.0 bits (162), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 71/271 (26%), Positives = 117/271 (43%), Gaps = 37/271 (13%) Query: 12 DGSYTEHAAVVLASIF-SKTEQTINVHIVHDDSLSEENKVKLTQMVDVFHHNIFFYHVTI 70 D ++ V +AS+ + E+ + H V D E++ + + + I Y + Sbjct: 35 DKNFLFGCGVSIASVLLANPEKALAFH-VFTDFFDSEDQQRFEALAKQYATQIVVYLIDC 93 Query: 71 PGDMREVAAGVHKIDYWTMASMYRLLLPIII--QTDRVIYLDCDILVNMDINELWSIDLG 128 E + WT A+ +R ++ +TDRV+YLD DI I EL ++ Sbjct: 94 -----ERLKSLPSTKNWTYATYFRFIIADYFSDKTDRVLYLDADIACKGSIQELIDLNFA 148 Query: 129 DRYLGAVLDQGENLLEYFTSMGLSAEL------YFNSGVILFHL-----DNIRKKDNWYG 177 + + AV+ +GE LE++T +S YFN+G IL ++ +NI KK Sbjct: 149 ENEIAAVVAEGE--LEWWTKRSVSLATPGLVSGYFNAGFILINIPLWTAENISKKA---I 203 Query: 178 EMLNFLRNFPKMTMPDQDVLNAVYSCNYLQLDQCFNT-FAQ---------NELDLEHKII 227 EML ++T DQDVLN +D+ FNT F+ N +D E + Sbjct: 204 EMLKDPEVVQRITHLDQDVLNIFLVNKARFVDKKFNTQFSLNYELKDSVINPVDAETVFV 263 Query: 228 HFAGENKWWDA--DSPHVTLYNNFRAMTPWG 256 H+ G K W + P + ++ +PW Sbjct: 264 HYIGPTKPWHSWGAYPVSQYFLQAKSNSPWS 294 >ZwaaJ [M] COG1442 Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases Length = 337 Score = 62.0 bits (149), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 68/269 (25%), Positives = 118/269 (43%), Gaps = 33/269 (12%) Query: 12 DGSYTEHAAVVLASIFSKTEQT-INVHIVHDDSLSEENKVKLTQMVDVFHHNIFFYHVTI 70 D +Y AA+ +ASI +Q HI+ D L +E L+Q+ + I YH+ Sbjct: 35 DENYQVGAAISIASILENNKQNKFTFHIIAD-YLDKEYIELLSQLATKYQTVIKLYHID- 92 Query: 71 PGDMREVAAGVHKIDYWTMASMYRLLL--PIIIQTDRVIYLDCDILVNMDINELWSIDLG 128 E + + + W ++ YRLL + D ++YLD DI+ +NEL +++ Sbjct: 93 ----SEPLKALPQSNIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFK 148 Query: 129 DRYLGAVLDQGENLLEYFTSMGLSAE----LYFNSGVILFHLDNIRKK--DNWYGEMLNF 182 D Y V+D + ++ ++ L E YFNSGV+ +L K+ + ++L+ Sbjct: 149 DEYGAVVIDV--DAMQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSD 206 Query: 183 LRNFPKMTMPDQDVLNAVYSCNYLQLDQCFNTFAQNELDLEHK--------------IIH 228 K+ PDQD+LN ++ + L + +N + + E K IH Sbjct: 207 ESIIKKLKYPDQDILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIH 266 Query: 229 FAGENKWWD--ADSPHVTLYNNFRAMTPW 255 + G K W A+ + N ++PW Sbjct: 267 YTGITKPWHDWANYASADYFRNIYNISPW 295 >ECs4502 [M] COG1442 Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases Length = 337 Score = 62.0 bits (149), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 68/269 (25%), Positives = 118/269 (43%), Gaps = 33/269 (12%) Query: 12 DGSYTEHAAVVLASIFSKTEQT-INVHIVHDDSLSEENKVKLTQMVDVFHHNIFFYHVTI 70 D +Y AA+ +ASI +Q HI+ D L +E L+Q+ + I YH+ Sbjct: 35 DENYQVGAAISIASILENNKQNKFTFHIIAD-YLDKEYIELLSQLATKYQTVIKLYHID- 92 Query: 71 PGDMREVAAGVHKIDYWTMASMYRLLL--PIIIQTDRVIYLDCDILVNMDINELWSIDLG 128 E + + + W ++ YRLL + D ++YLD DI+ +NEL +++ Sbjct: 93 ----SEPLKALPQSNIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFK 148 Query: 129 DRYLGAVLDQGENLLEYFTSMGLSAE----LYFNSGVILFHLDNIRKK--DNWYGEMLNF 182 D Y V+D + ++ ++ L E YFNSGV+ +L K+ + ++L+ Sbjct: 149 DEYGAVVIDV--DAMQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSD 206 Query: 183 LRNFPKMTMPDQDVLNAVYSCNYLQLDQCFNTFAQNELDLEHK--------------IIH 228 K+ PDQD+LN ++ + L + +N + + E K IH Sbjct: 207 ESIIKKLKYPDQDILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIH 266 Query: 229 FAGENKWWD--ADSPHVTLYNNFRAMTPW 255 + G K W A+ + N ++PW Sbjct: 267 YTGITKPWHDWANYASADYFRNIYNISPW 295 >SP1770 [M] COG1442 Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases Length = 404 Score = 62.0 bits (149), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 46/174 (26%), Positives = 79/174 (45%), Gaps = 25/174 (14%) Query: 88 TMASMYRLLLPIIIQTDRVIYLDCDILVNMDINELWSIDLGDRYLGAVLDQGENLLEYFT 147 + A+ +R +P ++ R +YLD DI+V ++ L+ I+L L AV D Sbjct: 81 SYATFFRYFIPNFVKESRALYLDSDIIVTGSLDYLFDIELDGYALAAVED---------- 130 Query: 148 SMGLSAELYFNSGVILFHLDNIRKKDNWYGEMLNFLRNFPKMTMPDQDVLNAVYSCNYLQ 207 S G FNSG++L ++D R +D ++L + + DQ +LN ++ + + Sbjct: 131 SFGDVPSTNFNSGMLLVNVDTWRDEDAC-SKLLELTNQYHETAYGDQGILNMLFHDRWKR 189 Query: 208 LDQCFNTFAQNELDLEHKIIHFAGENKWWD------ADSPHVTLYNNFRAMTPW 255 LD+ FN + + H G +KW++ D P V Y + PW Sbjct: 190 LDRNFNFMVGMD-----SVAHIEGNHKWYEISELKNGDLPSVIHYT---GVKPW 235 >SP1771_2 [M] COG1442 Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases Length = 437 Score = 60.1 bits (144), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 64/256 (25%), Positives = 113/256 (44%), Gaps = 45/256 (17%) Query: 16 TEHAAVVLASIFSKTEQ-------------TINVHIVHDDSLSEENKVKLTQMVDVFHHN 62 +E A+VLA+ + +Q +I +++H D +E K +L + ++ F Sbjct: 41 SEKKAIVLAANYGYVDQVLTTIKSICYHNRSIRFYLIHSDFPNEWIK-QLNKRLEKFDSE 99 Query: 63 IFFYHVTIPGDMREVAAGVHKIDYWTMASMYRLLLPIIIQTDRVIYLDCDILVNMDINEL 122 I VT +++ I Y R + +Q D+ +YLDCD++V ++++L Sbjct: 100 IINCRVT----SEQISCYKSDISY---TVFLRYFIADFVQEDKALYLDCDLVVTKNLDDL 152 Query: 123 WSIDLGDRYLGAVLDQGENLLEYFTSMGLSAELYFNSGVILFHLDNIRKKDNWYGEMLNF 182 ++ DL D L AV D F + FN+GV+L + + KK+N ++++ Sbjct: 153 FATDLQDYPLAAVRD--------FGGRAYFGQEIFNAGVLLVN-NAFWKKENMTQKLIDV 203 Query: 183 LRNF-PKMTMPDQDVLNAVYSCNYLQLDQCFNTFAQNELDLEHKIIH--FAGENKWWDAD 239 + K+ DQ +LN ++ +L+LD D H +IH FA D Sbjct: 204 TNEWHDKVDQADQSILNMLFEHKWLELD----------FDYNHIVIHKQFADYQLPEGQD 253 Query: 240 SPHVTLYNNFRAMTPW 255 P + Y + R PW Sbjct: 254 YPAIIHYLSHRK--PW 267 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.138 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,972,255 Number of extensions: 973697 Number of successful extensions: 1459 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 1450 Number of HSP's successfully gapped: 12 Length of query: 448 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 338 Effective length of database: 25,691,691 Effective search space: 8683791558 Effective search space used: 8683791558 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 117 (49.7 bits)